Hasil Pencarian  ::  Simpan CSV :: Kembali

"ABSTRAKMiR-21 diketahui berperan dalam proliferasi dan diferensiasi osteoklas, namun peran ekspresi miR-21 serum pada osteoporosis masih belum jelas. Penelitian sebelumnya mendapatkan bahwa ekspresi miR-21 serum berkorelasi positif dengan densitas mineral tulang pada penderita osteoporosis pascamenopause, tetapi penelitian tersebut tidak menganalisis faktor-faktor lainnya yang terlibat dalam osteoporosis pascamenopause.Penelitian ini bertujuan untuk mengetahui peran ekspresi miR-21 serum, konsentrasi RANKL, OPG, TGF- ? 1, sklerostin, rasio RANKL/OPG, kalsium serum dan aktivitas fisis terhadap densitas mineral tulang belakang pada perempuan pascamenopause hipoestrogenik dengan osteoporosis dibandingkan dengan tanpa osteoporosis, dengan point of interest pada ekspresi miR-21 serum.Penelitian ini dilakukan dengan disain uji potong lintang komparatif, di RSUD Ulin Banjarmasin, pada bulan Agustus 2015 sampai Juli 2016. Subjek dibagi menjadi 2 kelompok yaitu perempuan pascamenopause hipoestrogenik dengan osteoporosis dan tanpa osteoporosis. Pengambilan sampel dilakukan dengan metode consecutive. Pemeriksaan ekspresi miR-21 serum menggunakan metode absolute quantification real-time PCR. Analisis statistik menggunakan uji korelasi Spearman, Mann-Whitney U test dan regresi linear berganda.Subjek dibagi menjadi 2 kelompok yaitu perempuan pascamenopause hipoestrogenik dengan osteoporosis PMOP dan tanpa osteoporosis PMNOP masing-masing sebanyak 60 subjek. Median ekspresi miR-21 serum pada PMOP lebih tinggi secara bermakna dibandingkan dan PMNOP p = 0,001 . Ekspresi miR-21 serum, RANKL, rasio RANKL/OPG dan aktivitas fisis berkorelasi bermakna dengan nilai BMD pada PMOP. Aktivitas fisis sedang berkorelasi negatif bermakna dengan ekspresi miR-21 serum pada PMOP dan PMNOP. Analisis regresi linear berganda menggunakan metode backward stepwise mendapatkan persamaan regresi linear: BMD = 1,373 - 0,085 Ln.miR-21 - 0,176 Log 10.RANKL R2 = 52,5 .Simpulan. Ekspresi miR-21 serum pada perempuan pascamenopsuse hipoestrogenik dengan osteoporosis terbukti lebih tinggi dibandingkan dengan tanpa osteoporosis. Ekspresi miR-21 serum terbukti memberikan pengaruh negatif terhadap nilai BMD tulang belakang pada perempuan pascamenopause hipoestrogenik dengan osteoporosis sebesar 8,5 , dengan persamaan regresi linear BMD = 1,373 - 0,085 Ln.miR-21 - 0,176 Log10.RANKL. Persamaan ini dapat menjelaskan nilai BMD tulang belakang sebesar 52,5 . Kata kunci: BMD, miR-21 serum, Osteoporosis, Pascamenopause

---

ABSTRACTMiR-21 is known to play a role in osteoclast proliferation and differentiation, but the role of serum miR-21 expression in osteoporosis remains unclear. Previous research found that serum miR-21 expression was positively correlated with bone mineral density in postmenopausal osteoporosis patients, but the study did not analyze other factors involved in postmenopausal osteoporosis.This study aimed to determine the role of serum miR-21 expression, concentration of RANKL, OPG, TGF- ? 1, sclerostin and serum calcium, RANKL/OPG ratio, and physical activity on bone mineral density of spine in hypoestrogenic postmenopausal women with osteoporosis compared to no osteoporosis, with point of interest on the expression of serum miR-21.This study was conducted by comparative cross sectional design, conducted at Ulin General Hospital Banjarmasin, from August 2015 until July 2016. The subjects were divided into 2 groups of hypoestrogenic postmenopausal women with osteoporosis and without osteoporosis. Sampling was done by consecutive method. Examination of serum miR-21 expression using absolute quantification real-time PCR method. Statistical analysis using Spearman correlation test, Mann-Whitney U test and multiple linear regression.The subjects were divided into 2 groups of hypoestrogenic postmenopausal women with osteoporosis PMOP and without osteoporosis PMNOP each as many as 60 people. Median of serum miR-21 expression at PMOP group was significantly higher compared to PMNOP group p = 0.001 . Serum miR-21 expression, RANKL, RANKL/OPG ratio and physical activity were significantly correlated with BMD values ? ? ? ?in PMOP group. Moderate physical activity was significantly negative correlated with serum miR-21 expression. Multiple linear regression multivariate analysis using backward stepwise method obtained linear regression equation BMD = 1,373 - 0,085 Ln.miR-21 - 0,176 Log10.RANKL R2 = 52,5 .Conclusion. Serum miR-21 expression in hypoestrogenic postmenopausal women with osteoporosis has been shown to be higher compared with no osteoporosis. Serum miR-21 expression proved to have a negative effect on spinal BMD values in hypoestrogenic postmenopausal women with osteoporosis of 8.5 , with linear regression equation BMD = 1.373 - 0.085 Ln.miR-21 - 0.176 Log10.RANKL. This equation can explain the value of spinal BMD by 52.5 . Keywords: BMD, Osteoporosis, postmenopausal, serum miR-21 "

"During three months ,August 1991 until November 1991, the randomized clinical trial study was conducted to elucidate the effect of calcium supplementation on calcium metabolism to prevent bone loss ip postmenopausal women. Thirty four postmeno- pausal women age 55 to 65 years old from RN. 01, Panjunan village, Astanaanyar subdistrict, Bandung regency West Java who had undergone menopause at least five years since last menses were recruited to the study. The subjects were randomly assigned to the treatment (1000 mg calcium/day) and the control groups in a double blind designs.Thirty one subjects who remained in the study consist of sixteen treatment subjects and fifteen control subjects were included in the final data analysis. Baseline data of the study showed no a significant difference for all variables, therefore baseline data were homogenous. Based on two x 24 hours dietary recall methods all subjects had low dietary calcium intake (291.5l mg/ day and 289.32 mg/day).After treatment, based on the laboratory tests show that the serum calcium concentration was significantly increased in the treatment group (p<0.001) and was not significant difference (p=O.596) in the control group. Serum calcium was a significant difference (p<0.001) between the treatment and the control group. Parathyroid hormone level was significantly decreased in the treatment group (p<0.05) and was significantly increased in the control group (p<0.01). There was significant difference (p<0.00l) between the treatment and the control group. Bone loss of radius and ulna were determined by z-ray standard measurement were significantly reduced (p<0.001 and p<0.01, respectively) after 1000 mg calcium supplementation. Alkaline phosphatase was significantly decreased (p<0.001) in the treatment group and was significantly increased in the control group. There was significant difference (p<0.001) between the treatment and the control group. Urinary ca}cium creatinine ratio was not significantly increased (pI0.764 and p=0.0BB) and was not significant difference (p=0.067) in all subjects after treatment. This study support the hypothesis that calcium supplementation can maintain calcium balance, even improved bone density in postmenopausal women."

"ABSTRAKLatihan naik turun bangku LNTB dan latihan jalan kaki LJK adalah aktivitas bersifat weight bearing yang dapat meningkatkan massa tulang. Kedua latihan fisik tersebut memiliki karakter biomekanik yang berbeda yang akan memengaruhi proses formasi dan resorbsi tulang. Densitas massa tulang rendah berhubungan dengan polimorfisme gen TNFSF11 dan TNFRSF11B.Penelitian ini bertujuan untuk menganalisis pengaruh perbedaan peningkatan kadar osteokalsin sebagai petanda formasi dan penurunan CTX-1 sebagai petanda resorbsi setelah LNTB dan LJK dan pengaruh polimorfisme gen TNFSF11 SNPs-290C>T, -643C>T, -693G>C dan gen TNFRSF11B SNPs 163A>G, 950T>C, 1181G>C terhadap perbedaan peningkatan kedua petanda. Disain penelitian adalah studi eksperimental. Subjek penelitian adalah perempuan osteopenia sebanyak 59 orang yang diberi LNTB 30 subjek dan LJK 29 subjek dan ditentukan secara acak. Kadar osteokalsin dan serum diukur pra dan pascalatihan setelah 12 sesi selama 3 bulan latihan. Identifikasi polimorfisme gen TNFSF11 dan gen TNFRSF11B dianalisis dengan metode PCR dilanjutkan RFLP. Perbedaan peningkatan kadar osteokalsin dan CTX-1 diuji dengan uji T-tidak berpasangan. Hubungan polimorfisme dengan perubahan kadar osteokalsin dan CTX-1 dianalisis dengan odds ratio. Analisis haplotype dan uji Kruskall Wallis dilakukan untuk melihat hubungan pasangan haplotype dengan kadar osteokalsin dan CTX-1. Kadar osteokalsin dan CTX-1 setelah latihan pada kedua kelompok latihan meningkat p < 0,05 . Peningkatan CTX-1 setelah LNTB lebih kecil dibanding LJK p < 0,05 . Tidak ditemukan hubungan antara polimorfisme gen TNFSF11 dan gen TNFRSF11B dengan perubahan kadar osteokalsin dan CTX-1 p>0,05 Meskipun tidak bermakna, terdapat kecenderungan alel heterozigot/resesif berhubungan dengan peningkatan kadar osteokalsin, alel homozigot dominan berhubungan penurunan kadar CTX-1, serta haplotype gen TNFRSF11B dan haplotype gen TNFSF11 dengan perubahan osteokalsin dan CTX-1. Kedua latihan fisik terbukti dapat meningkatkan formasi tulang. LNTB mencegah resorbsi tulang lebih baik dibanding LJK. Polimorfisme mungkin memengaruhi formasi dan resorbsi tulang akibat latihan fisik. LNTB dan LJK meningkatkan osteokalsin. Peningkatan CTX-1 setelah LNTB lebih rendah dari LJK. Perubahan osteokalsin tidak berhubungan dengan polimorfisme gen TNFSF11 dan TNFRSF11B sedangkan perubahan CTX-1 tidak dipengaruhi polimorfisme gen TNFSF11 tetapi terdapat hubungan antara haplotype ATG dengan ACG dan GCC gen TNFRSF11B dengan perubahan CTX-1. Kata Kunci: gen TNFRSF11B, gen TNFSF11, karakter biomekanik, latihan naik turun bangku, polimorfisme, remodeling tulang

---

Bench step exercise BSE and walking exercise WE have a different biomechanical characteristics that can affect bone formation and resorbtion. Low bone mass density was associated with TNFSF11 SNPs 290C T, 643C T, 693G C and TNFRSF11B 163A G, 950T C, 1181G C genes polymorphism.The purpose of the studi is to analyze the difference effect of BSE and WE on changes in bone formation osteocalcin and bone resorbtion CTX 1 markers and the association of TNFSF11 and TNFRSF11B genes polymorphism. This is an experimetal study involving 59 postmenopausal women with osteopenia. Participants were grouped randomly according to the type of exercise bench step exercise 30 subjects and walking exercise 29 subjects . Subjects performed exercise for 12 sessions in 3 months. Osteocalcin and CTX 1 serum was measured pre and post exercise. The TNFSF11 gene and TNFRSF11B gene polymorphism was identified by PCR and RLFP methods. The difference in changes on osteocalcin and CTX 1 between groups were analysed by independent T test. Association between changes in osteocalcin and CTX 1 polymorphism and haplotype were analysed using odss ratio and Kruskall Wallis, respectively. Osteocalcin and CTX 1 increased significantly after BSE and WE P 0.05 , with no difference between group. BSE increased CTX 1 lower than WE P 0.05 . There is no association between polymorphism and the changes on osteocalcin and CTX 1 levels after BSE and WE P 0.05 . However, some tendencies were observed. Heterozygous recessive alleles had association with increased osteocalcin, homozygous dominant alleles had association with decreased CTX 1, haplotype TNFRSF11B gene had associaton with changes in osteocalcin and CTX 1 levels, whereas haplotype TNFSF11 gene with changes in CTX 1 level only. This results indicate that both BSE and WE increased bone formation. TNFSF11 gene polymorphism did not affect changes in formation and resorbtion after BSE and WE. Both exercises increased osteocalcin but was not different even though level of CTX 1 in BSE is lesser compared to WE. TNFSF11 gene polymorphism did not associate to the changes in osteocalcin and TNFRSF11B gene polymorphism did not associate to the changes in osteocalcin despite haplotype ATG had association with ACG and GCC in TNFSF11B gene for the changes in CTX 1 level after BSE and WE. Keywords Bench step exercise, biomechanical character, bone remodelling, gene TNFRSF11B, gene TNFSF11, polymorphism"

"Today jawbone radiographic studies have shifted to 'bone quality'. The jawbone quality indicator which is often used is trabeculation density. To obtain accurate interpretation, analysis of jawbone quality needs modern radiographic imaging modalities that still very limited in Indonesia. In prosthetic dentistry analysis of tarabecular bone density in postmenopause women is very important to anticipate the possibility of osteoporotic cases. Because of inadequacy of human eye sight, information obtained from plain conventional radiographic is very limited. With digitized conventional radiogram, dentist could obtain more accurate information to analyze radiographic trabeculation density in Indonesia. The aim of this study is to get more quantitative radiographic data from digitized conventional radiogram comparing those obtained from conventional radiogram. 112 conventional radiograms were evaluated using grading of trabeculation density. The same radiograms then digitized to obtain the quantitative numeric data of trabeculation density. The result showed that each grade from conventional radiogram has a range of numeric value in the digitized radiogram. This result could provide higher accuracy of trabeculation density analysis than the value obtained by using human eyes that will be beneficial for the dentist, especially prosthodontist in Indonesia."

"Ekstrak kulit buah delima yang mengandung asam elagat saat ini sedang dikembangkan menjadi terapi alternatif sebagai pengganti hormon untuk wanita pascamenopause. Asam elagat memiliki aktifitas estrogen pada tikus yang di ovariektomi. Namun belum diketahui apakah kandungan ekstrak kulit buah delima dapat memberikan efek estrogenik pada wanita pascamenopause. Oleh karena itu dilakukan penelitian untuk menilai pengaruh efek estrogenik pada kulit buah delima terhadap wanita pascamenopause.Hipotesis penelitian ini adalah ekstrak kulit buah delima dapat memperbaiki keluhan klimakterik, kadar hormon estradiol dan FSH pada wanita pascamenopause. Penelitian ini merupakan uji klinik acak terkontrol,plasebo, buta ganda, paralel, pada 30 subyek wanita pascamenopause. Subyek menerima kapsul placebo atau ekstrak kulit buah delima 1100mg/hari selama 8 minggu. Sebelum dan sesudah perlakuan dilakukan pemeriksaan terhadap subyek penelitian wawancara keluhan klimkaterik menggunakan questioner Menopause Rating Scale/MRS serta pemeriksaan hormon estradiol dan FSH.Penelitian ini menunjukkan bahwa pemberian ekstrak kulit buah delima dapat memperbaiki keluhan klimakterik terutama keluhan psikologikal, serta terdapat kecenderungan perbaikan kadar hormon estradiol dan FSH pada wanita pascamenopause, tidak ditemukan kejadian tidak diharapkan dan perubahan parameter laboratorium pada seluruh subyek penelitian. Dari penelitian ini dapat diambil kesimpulan bahwa ekstrak kulit buah delima dapat bersifat sebagai fitoestrogen pada wanita pascamenopause dan bersifat aman.

---

Pomegranate (Punica granatum L.) peel extract contain elagat acid currently being developed as an alternative as hormone replacement therapy for postmenopausal women. Elagat acid has been proved estrogen activity on ovariectomized rats. However, it has not been discovered yet whether extract pomegranate (Punica granatum L.) peel could provide estrogenic effects on postmenopausal women. Therefore this study to assess the effect of estrogenic effects on the pomegranate (Punica granatum L.) peel for postmenopausal women.The hypothesis of this study is the pomegranate peel extract could improve climacteric complaints, estradiol and FSH hormone levels in postmenopausal women. This study is a randomized, controlled clinical trial, placebo, double blind, parallel, on 30 subjects postmenopausal women. Subjects receiving placebo or 1100mg / day capsules of pomegranate (Punica granatum L.) peel extract for 8 weeks. An interview using a Menopause Rating Scale / MRS questionnaire and examinations of hormones estradiol and FSH were conducted before and after the treatment carried out.This study showed that administration of pomegranate (Punica granatum L.) peel extract can improve climacteric complaints, especially psychological complaints, and there was a tendency of estradiol and FSH hormone levels improvement to postmenopausal women, there were no such unexpected events and changes in laboratory parameters in all study subjects. From this study, we could stated that pomegranate (Punica granatum L.) peel extract could safely act as phytoestrogens in postmenopausal women."

"Pendahuluan: Salah satu faktor risiko penyakit osteoporosis pada wanita menopause adalah faktor genetik polimorfsme Tumor Necrosis Factor Alpha TNF-α. Tujuan: Penelitian ini bertujuan untuk melihat ada atau tidaknya polimorfisme dan perbedaan polimorfisme gen TNF-α-308G/A pada wanita pascamenopause dengan osteoporosis. Metode: 100 bahan biologis tersimpan 50 sampel wanita pascamenopause dengan osteoporosis dan 50 sampel individu sehat dianalisa menggunakan teknik PCR-RFLP dengan enzim retriksi NcoI, selanjutnya data diuji secara statistik menggunakan uji Chi-square. Hasil: Ditemukan banyak genotip AG baik pada kelompok osteoporosis dan kontrol. Pada kelompok osteoporosis tidak ditemukan genotip GG dan terdapat 76 genotip AG serta 24 genotip AA. Pada kelompok kontrol, terdapat 8 genotip GG, 82 genotip AG, dan 5 genotip AA. Kesimpulan: Terdapat polimorfisme genetik TNF-α-308G/A pada wanita menopause dengan osteoporosis, namun tidak terdapat perbedaan bermakna pada polimorfisme antara wanita pascamenopause dengan osteoporosis dan individu sehat p = 0.117 di populasi Indonesia.

---

Introduction: One of the risk factor for osteoporosis in postmenopausal woman is genetic polymorphism factor which is Tumor Necrosis Factor Alpha TNF.

Objectives: This research aims to look for genetic polymorphism and differentiate the distribution TNF 308G A gene polymorphism in postmenopausal woman with osteoporosis.

Methods: 100 stored biological samples 50 samples of postmenopausal woman with osteoporosis and 50 healthy control samples were analyzed with PCR RFLP technique using NcoI restriction enzyme, and subsequently assessed with statistical analysis using Chi square test.

Result: AG genotype was found with the highest amount in both samples. The postmenopausal group has 76 of AG genotype, 24 of AA genotype, and no GG genotype was found. The healthy control group has 8 of GG genotype, 82 of AG genotype, and 5 of AA genotype. Based on Fisher Extract test, there is no significant association between TNF 308G A and postmenopausal osteoporosis p value 0.117.

Conclusion: The genetic polymorphism of TNF 308G A in postmenopausal woman was found, but the polymorphism didn rsquo t have any association with osteoporosis in Indonesia populations. "

"One of the major health problems in elderly women is osteoporosis post menopause. Dentists must be aware of the disease since its involvement on the jaw. Nowadays, there are a lot of researches on correlation of osteoporosis and mandible but only few concentrate on hormonal substitution therapy and/or Calcium-vitamin D3 (Ca-vit D3). This research is to evaluate the effect of hormonal substitution therapy and/or Ca-vit D3 on mandibular height. Forty five rats are used in this research and divided into nine groups: one control group, two ovariectomized (OVX) groups, two OVX groups treated with estrogen, two OVX groups treated with Ca-vit D3, two OVX groups treated with estrogen and Ca-vit D3. All of the rats except the control groups were ovariectomized as model for pstmenopausal estrogen deficiency state. The treatment was done in two or four weeks. The animals were killed with cervical discoloration, the mandible were excised and soaked on Hydrogen Peroxide 10%. Then the mandible's heights on right buccal side were measured from the mandibular base to the alveolar crest. It is concluded that hormonal substitution therapy and combination of the hormonal substitution therapy and Ca-vit D3 can maintain the normal mandibular height. Mandibular height of groups with therapy using Ca-vit D3 have slightly lower means compared to control group but without significant difference statistically. The best therapy is combination of hormonal substitution therapy and Ca-vit D3."

"Osteoporosis is a silent disease, i.e. the disease will progress without symptoms or pain until bones start to break. Densitometry examinations to diagnose osteoporosis is relatively expensive for most people in Indonesia. Kusdhany et al have produced a mandibular density index specific for Deutero-Malay postmenopausal women (POSTUR-P). This index can be used to predict osteoporosis risk in mandibular and other bones. The objective of this research was to try out POSTUR-P before using it in the community. The study was a diagnostic test, on 31 Teratai clinic postmenopausal patients of Ciptomangunkusumo Hospital between 50-75 years of age from June to October 2014 and fulfilling inclusion criteria. Densitometry on lumbal and femur bones was used as a gold standard. The result of this study showed that POSTUR-P has high sensitivity and moderate specificity, and appears to be a good screening tool to indicate osteoporosis cases."

"The need for women to use a dental prosthesis increases with age. Low estrogen level is characteristic in post menopausal women. In this case, it often related with reduction of density and mandibular alveolar bone height. This study aimed to investigate the differences in density and mandibular alveolar bone height in post-menopausal women wearing complete dentures with tissue conditioner. The clinical observations were conducted on seven persons who had received a new set of complete denture in the Prosthodontic Department of the Dental Hospital of the Dental Faculty in Jember University. Balanced occlusion was used in all dentures, fabricated according to the principles used in the department. Panoramic radiographs had been made for all subjects as the baseline, and two, four, and six months later using dentures with tissue conditioner. The data was analyzed by using one-way analysis of variance at the 0.05 level. The conclusion was that there is no significant difference in density and mandibular alveolar bone height of complete denture wearers with tissue conditioner in post-menopausal women."

"Indonesian Journal of Dentistry 2006: Edisi Khusus KPPIKG XIV: 148-154Removable Partial Denture Management on Post-Menopause Osteoporotic FemaleOsteoporosis a condition of generalized skeletal fragility caused by diminishing bone amount and disturbance in bone microarchitecture with implication that the bone ability to withstand forces is decreased.This condition leads to more bone resorption. Some researches show that this condition also affects jaw bone. Therefore we must take this condition into consideration during prosthodontic treatment. Dental prostheses are made to restore stomatognatic function as well to preserve what was left including residual alveolar bone. The ability ofdental prostheses to preserve residual alveolar bone differs according to type of prostheses. The case presented here proposing the treatment of an osteoporotic old female in Prosthodontic Clinic FKG Ul with removable partial denture. Considerations inselecting the type and design of dental prostheses and attempts to modif, factors that might play a role in bone resorption in connection with patient's physical and psychological status will be discussed. Thorough examination, careful planning and good communication with patient can provide optimum result."