Results: From a total of 1706 neonates, the incidence of proven LONS was 15,4%. It was more prevalent (58.4%) in preterm neonates aged less than 37 weeks than in term (41.6%) neonates. Majority (67.6%) of proven LONS subjects were neonates with low birth weight (<2500 grams) and the largest percentage between them (35.1%) were in ‘low birth weight (≥1500- <2500 grams)’ group. Gram negative bacteria have emerged as predominant pathogens of LONS patients in our hospital, with most common were Klebsiella pneumonia, Acinetobacter spp., Escherichia coli, Enterobacter spp., and Pseudomonas aeruginosa. Conclusions: The incidence of LONS in 2020 is 15,4%. The proportion of LONS among LBW and preterm neonates is significantly higher compared to normal birth weight and term neonates. Both LBW and NBW neonates, preterm and term neonates suffering LONS in CMH’s neonatal unit are mostly caused by gram-negative bacteria and this finding is statistically significant. Different pathogens causing LONS in this study can be utilized further to analyse the susceptibility of these pathogens to the current empirical antibiotic guideline used in CMH."