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"Two flavonoid compounds, 5,7,3?,4?-tetrahydroxy-6-geranylflavonol (1) and kaempferol 7-O-β-glucose (2) have beenisolated from the leaves of Macaranga hispida (Blume), Mull.Arg. Isolation and purification were conducted bychromatography methods and chemical structure characterization was carried out by spectroscopic methods. The5,7,3?,4?-tetrahydrxyi-6-geranyl flavonol (1) and kaempferol 7-O-glucose (2) had moderate cytotoxic activity againstmurine leukemia P-388 cell lines with IC50 value of 0.22 and 101.5 μg/mL, respectively. The IC50 for antioxidantactivities of (1) and (2) were 2.83 and 13.95 μg/mL, respectively. The LC50 of (1) and (2) from BSLT were 350 and>1000 μg/mL, respectively.Identifikasi dan Studi Bioaktifitas Senyawa Flavonoid dari Macaranga hispida (Blume) Mull.Arg. Dua senyawaflavonoid, 5,7,3?,4?-tetrahidroksi-6-geranilflavonol (1) dan kaemferol 7-O-β-glukosa (2) telah diisolasi dari daunMacaranga hispida (Blume) Mull. Arg. Isolasi dan pemurnian dilakukan dengan metode kromatografi dan karakterisasistruktur kimia dilakukan dengan metode spektroskopi. 5,7,3', 4'-tetrahidroksi-6-geranil flavonol (1) kaemferol 7-Oglukosa(2) memiliki aktivitas sitotoksik sel murine leukemia P-388 dengan nilai IC50 masing-masing 0,22 dan 101,5μg/mL. Aktivitas antioksidan (1) dan (2) dinyatakan dengan nilai IC50 masing-masing sebesar 2,83 dan 13,95 μg/mL.Nilai LC50 dengan uji BSLT (1) dan (2) masing-masing 350 dan >1000 μg/mL."

"Lovastatin is an anti-cholesterol agent that was produced by Aspergillus terreus using solid state fermentation (SSF). During this fermentation process, sulochrin is also produced as an unwanted co-metabolite. However, our previous result showed that sulochrin had potential as antidiabetes because it is an inhibitor agent of α-glucosidase. In this paper, we reported our observation on lovastatin and sulochrin production pattern in relation with inhibitor α-glucosidase activity during eleven days fermentation of A. terreus koji (SSF) ethyl acetate extract. Koji obtained from solid state fermentation with rice as the substrate and incubated at room temperature, sample is taken daily for eleven day (D-1 toD-11). Lovastatin and sulochrin production was measured by Liquid Chromatography- Mass Spectrometer based on their molecular weight m/z 404.5 and 332.3 respectively. The in hibitory activity is measured by inhibition model of koji extract against α-glucosidase (EC 3.2.1.20) from Saccharomyces cereviceae. The results show that lovastatin production was started on the day 2 (0.04 mg/g) and achieving the optimal production on day 7 (11.46 mg/g), while sulochrin production was started on day 4 (0.60 mg/g) and keep produced until the end of fermentation period at Day 11 (3.11 mg/g). Koji extract was started to show inhibitory to α-glucosidase activity on Day 5 (IC50= 23.34 μg/mL) and keep showed activity until Day 11 (IC50=3.33 μg/mL). These results suggest that inhibitory activity of koji extract to α-glucosidase activity have relation with sulochrin biosynthesis production. "