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Hasil Pencarian

Ditemukan 4 dokumen yang sesuai dengan query
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Iin Maemunah
"Bakteri Acinetobacter baumannii, Pseudomonas aeruginosa dan Klebsiella pneumoniae resisten multiobat penghasil biofilm merupakan masalah kesehatan serius di seluruh dunia, terutama di Indonesia sebagai negara dengan prevalensi bakteri Gram negatif resisten multiobat penghasil enzim beta laktamase tertinggi se-Asia Tenggara. Tujuan penelitian ini adalah mendapatkan metode deteksi dan mengetahui proporsi pembentukan biofilm dengan metode tabung eppendorf polypropylene yang mudah dikerjakan, reproducible dan efisien pada isolat simpan A. baumannii, P.aeruginosa dan K. pneumonia yang resisten multiobat penghasil biofilm di Laboratorium Mikrobiologi FKUI-RSCM pada periode Maret 2015-Oktober 2016. Sebanyak 71 isolat simpan yang menjadi sampel penelitian. Setiap isolat dilakuan uji deteksi pembentukan biofilm dengan media luria bertani, diinkubasi selama 30 jam, dibersihkan dari sisa sel planktonik dengan PBS steril dan diwarnai dengan crystal violet 0,1. Proporsi pembentukan biofilm pada Acinetobacter baumannii, Pseudomonas aeruginos dan Klebsiella pneumoniae masing-masing secara berurutan adalah 55,3, 53,3 dan 0. Metode deteksi pembentukan biofilm dengan tabung eppendorf polypropylene merupakan metode deteksi yang mudah dikerjakan, reproducible dan efisien, sehingga dapat dilakukan di laboratorium mikrobiologi klinik sederhana. Proporsi bakteri penghasil biofilm adalah lebih dari 50 non-Enterobactericeae resisten multiobat, tetapi tidak pada isolat K. pneumoniae resisten multiobat.

Biofilm forming multidrug resistant Acinetobacter baumannii, Pseudomonas aeruginosa and Klebsiella pneumoniae are a major cause of health problems in hospitalized facilities in the world, especially in Indonesia as the highest prevalence of the multidrug resistant Gram negative bacteria in Southeast Asia. The aim of this study is to get the method of detection and proportion of biofilm formation using polypropylene eppendorf tube because easy to do, reproducible and efficient from stock culture isolates of multidrug resistant A. baumannii, P.aeruginosa and K. pneumonia at clinical microbiology laboratory of FKUI RSCM during March October 2016. The are 71 stock culture isolates from the total sample, medium for biofilm cultivation was Luria Bertani, incubated for 30 hrs, washed with sterile phosphat buffer saline and stained with crystal violet 0,1. Proportion of biofilm producing of multidrug resistant Acinetobacter baumannii, Pseudomonas aeruginosa and Klebsiella pneumoniae were respectively 55.3, 53.5 and 0. Detection of biofilm formation using polypropylene eppendorf tube could perfom in simple clinical microbiology laboratory because easy to do, reproducible and efficient. The proportion of biofilm producing showed that more than 50 of multirug resistant of non Enterobactericeae, but no one of K. pneumoniae isolate.
"
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2018
T58851
UI - Tesis Membership  Universitas Indonesia Library
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Iin Maemunah
"Latar Belakang: Bakteri Acinetobacter baumannii, Pseudomonas aeruginosa dan Klebsiella pneumoniae resisten multiobat penghasil biofilm merupakan masalah kesehatan serius di fasilitas kesehatan di seluruh dunia, terutama di Indonesia sebagai negara dengan prevalensi bakteri Gram negatif penghasil enzim extended beta-laktamase ESBL tertinggi di atas rerata diantara berbagai negara di Asia Pasifik, belum memiliki data tentang bakteri Gram negatif penghasil biofilm. Diperlukan metode deteksi pembentukan biofilm dengan tabung eppendorf polypropelene yang mudah dikerjakan, reproducible dan efisien sehingga dapat dikerjakan di laboratorium mikrobiologi klinik sederhana.
Tujuan: Melakukan optimasi deteksi biofilm menggunakan metode tabung eppendorf polypropelene dan mengetahui proporsi pembentukan biofilm pada isolat simpan A. baumannii, P.aeruginosa dan K. pneumonia yang resisten multiobat penghasil biofilm di Laboratorium Mikrobiologi Klinis, Dept. Mikrobiologi FKUI-RSCM pada periode Maret 2015-Oktober 2016.
Metode: Penelitian dilakukan dalam 2 tahap. Tahap pertama adalah optimasi metode deteksi pembentukan biofilm dengan metode Cernohorska dkk menggunakan tabung eppendorf polypropylene. Pada tahap pertama ini dilakukan optimasi terhadap jenis media, masa inkubasi statis, bahan pencuci dari sisa-sisa sel planktonik dan konsentrasi crystal violet. Tahap kedua adalah mendapatkan proporsi bakteri penghasil biofilm dari penerapan metode deteksi pembentukan biofilm dengan tabung eppendorf polypropylene yang sudah dioptimasi terhadap 71 isolat simpan bakteri A. baumannii, P. aeruginosa dan K. pneumoniae resisten multiobat.
Hasil: Jenis media terbaik yang digunakan adalah Luria Bertani, masa inkubasi statis 30 jam, bahan pencuci dengan PBS steril dan konsentrasi crystal violet 0,1 untuk optimasi bakteri kontrol penghasil dan bukan penghasil biofilm. Proporsi pembentukan biofilm pada A. baumannii, P. aeruginos dan K. pneumoniae masing-masing secara berurutan adalah 55,3 , 53,3 dan 0.
Kesimpulan : Metode deteksi pembentukan biofilm dengan tabung eppendorf polypropylene merupakan metode deteksi yang mudah dikerjakan, reproducible dan efisien, sehingga dapat dilakukan di laboratorium mikrobiologi klinik sederhana. Proporsi bakteri penghasil biofilm adalah lebih dari 50 non-Enterobactericeae resisten multiobat, tetapi tidak pada isolat K. pneumoniae resisten multiobat.

Background: Biofilm forming multidrug resistant Acinetobacter baumannii, Pseudomonas aeruginosa, and Klebsiella pneumoniae are a major cause of health problems in hospitalized facilities in the world, especially in Indonesia as the highest prevalence of the extended beta lactamase producing multidrug resistant Gram negative bacteria in Southeast Asia. Therefore, it is important to apply biofilm detection using polypropylene eppendorf tube method as easy to do, reproducible and efficient to perform in the simple clincal microbiology laboratory.
Objective: To conduct optimization of biofilm detection using polypropylene eppendorf tube method and proportion of biofilm formation from stock culture isolates of multidrug resistant A. baumannii, P.aeruginosa and K. pneumonia at clinical microbiology laboratory of FKUI RSCM during March October 2016.
Method: This study was conducted in two phases. The first is optimization of biofilm formation method by Cernohorska et al using polypropylene eppendorf tube. The optimization is conducted in some conditions, such as medium for biofilm cultivation, long static incubation, washing solution and crystal violet concentration. The second is to get proportion of biofilm producing bacteria through application of optimized detection of biofilm formation method using polypropylene eppendorf tube to a total of 71 stock culture isolates of multidrug resistant A. baumannii, P.aeruginosa and K. pneumonia.
Results: The best medium for biofilm cultivation was Luria Bertani, long static incubation was 30 hours, washing solution was sterile PBS and crystal violet 0,1 for biofilm and non biofilm producing bacteria. Proportion of biofilm producing of multidrug resistant Acinetobacter baumannii, Pseudomonas aeruginosa and Klebsiella pneumoniae were respectively 55.3 , 53.5 and 0.
Conclusions: Detection of biofilm formation using polypropylene eppendorf tube could perfom in simple clinical microbiology laboratory because easy to do, reproducible and efficient. The proportion of biofilm producing showed that more than 50 of multirug resistant of non Enterobactericeae, but no one of multidrug resistant K. pneumoniae.
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Jakarta: Fakultas Kedokteran Universitas Indonesia, 2018
SP-Pdf
UI - Tugas Akhir  Universitas Indonesia Library
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Rony Satrio Utomo
"[ABSTRAK
Pendahuluan
Hipertensi merupakan masalah kesehatan dunia karena jumlah penderita yang
banyak serta komplikasi yang diakibatkannya. Pengendalian tekanan darah pada
pada pasien hipertensi masih belum adekuat. Penyebab utama kegagalan
pengendalian tekanan darah pada pasien hipertensi adalah ketidak-patuhan berobat,
adanya therapeutic inertia dan penyakit yang resisten.
Tujuan
Mengetahui proporsi therapeutic inertia pada pasien dengan tekanan darah tidak
terkontrol, tingkat medication adherence dan proporsi pengendalian tekanan darah
pada pasien hipertensi yang telah berobat lebih dari enam bulan dengan melakukan
pengisian kuesioner mengenai kepatuhan berobat dan evaluasi dari rekam medis
mengenai tatalaksana hipertensi.
Metode
Telah dilakukan penelitian potong lintang pada bulan April 2015 sampai Mei 2015
terhadap 126 pasien dengan hipertensi dan telah berobat lebih dari enam bulan di
poliklinik Ginjal-Hipertensi RSCM Jakarta-Indonesia. Subjek dilakukan
wawancara terstruktur dan pengukuran tekanan darah dan diminta untuk mengisi
kuesioner 8-item Morisky Medication Adherence Score (MMAS-8) untuk menilai
kepatuhan berobat serta evaluasi rekam medis pasien untuk menilai tatalaksana
hipertensi yang diterima, serta tekanan darah selama berobat.
Hasil
Didapatkan 113 subjek yang sesuai dengan kriteria penelitian. Dari hasil penelitian
didapatkan pengendalian tekanan darah adalah sebesar 69,3% dari seluruh
kunjungan pada pasien dengan tekanan darah tinggi, dari 30,7% pasien dengan
tekanan darah tidak terkontrol, tingkat therapeutic inertia mencapai 84,1%.
Kepatuhan berobat yang baik didapatkan pada 85,8% pasien dengan hipertensi
Simpulan
Tingkat pengendalian tekanan darah pada pasien hipertensi sudah cukup baik.
Kepatuhan berobat pasien dengan hipertensi sudah baik. Tingkat therapeutic
inertia pada pasien hipertensi dengan tekanan darah tidak terkontrol masih tinggi.

ABSTRACT
Background
Hypertension is a worldwide medical problem because of huge amount of
hypertensive patient and complication tha follows. The blood pressure control of
hypertensive patients is inadequate. The main reason failure in controlling blood
pressure of hypertensive patient are medication inadherent, therapeutic inertia and
resistant disease.
Objectives
To determine the proportion of therapeutic inertia in hypertensive patient with
uncontrolled blood pressure, medication adherence level and blood pressure
control rate in hypertensive patient who has been on medication for over than six
month by filling questionnaire on medication adherence and evaluation of medical
record on hypertension therapy.
Method
A cross-sectional study was conducted in April 2015 through May 2015 on 126
hypertensive patient and has been on hypertension medication for over than six
month at Nephrology-Hypertension clinic Cipto Mangunkusumo Hospital, Jakarta-
Indonesia. We conducted structured interview and blood pressure measurement to
the subject, and requested to fill 8-item Morisky Medication Adherence Score
(MMAS-8) to evaluate medication adherence and reveiw of medical record to
evaluate hypertension therapy and blood pressure during ambulatory visit.
Results
There were 113 subject that meet the study criteria. The blood pressure control rate
were 69.3% from all visit of hypertensive patient. From 30.7% visit with
uncontrolled blood pressure, therapeutic inertia were 84,1%. Good medication
adherence were found in 85.8% hypertensive patient.
Conclusion
Blood pressure control rate in hypertensive patient is good. Medication adherence
in hypertensive patient were also found good. We found that the theraputic inertia
level among hypertensive patient with uncontrolled blood pressure is high., Background
Hypertension is a worldwide medical problem because of huge amount of
hypertensive patient and complication tha follows. The blood pressure control of
hypertensive patients is inadequate. The main reason failure in controlling blood
pressure of hypertensive patient are medication inadherent, therapeutic inertia and
resistant disease.
Objectives
To determine the proportion of therapeutic inertia in hypertensive patient with
uncontrolled blood pressure, medication adherence level and blood pressure
control rate in hypertensive patient who has been on medication for over than six
month by filling questionnaire on medication adherence and evaluation of medical
record on hypertension therapy.
Method
A cross-sectional study was conducted in April 2015 through May 2015 on 126
hypertensive patient and has been on hypertension medication for over than six
month at Nephrology-Hypertension clinic Cipto Mangunkusumo Hospital, Jakarta-
Indonesia. We conducted structured interview and blood pressure measurement to
the subject, and requested to fill 8-item Morisky Medication Adherence Score
(MMAS-8) to evaluate medication adherence and reveiw of medical record to
evaluate hypertension therapy and blood pressure during ambulatory visit.
Results
There were 113 subject that meet the study criteria. The blood pressure control rate
were 69.3% from all visit of hypertensive patient. From 30.7% visit with
uncontrolled blood pressure, therapeutic inertia were 84,1%. Good medication
adherence were found in 85.8% hypertensive patient.
Conclusion
Blood pressure control rate in hypertensive patient is good. Medication adherence
in hypertensive patient were also found good. We found that the theraputic inertia
level among hypertensive patient with uncontrolled blood pressure is high.]"
2015
T-Pdf
UI - Tesis Membership  Universitas Indonesia Library
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Ruth Vonky Rebecca
"Identifikasi IL-6, PCT, dan CRP dalam mendiagnosis awal bakteremia pada pasien leukemia akut dengan demam neutropenia banyak dilakukan. Tujuan studi ini mengetahui performa diagnostik delta IL-6, delta PCT, dan delta CRP sebagai penanda awal kejadian bakteremia pada pasien leukemia akut dewasa dengan neutropenia berat selama menjalani kemoterapi standar (agresif). Desain studi ini potong lintang dengan mengambil semua pasien leukemia akut dewasa yang mengalami neutropenia berat selama menjalani kemoterapi agresif di RS Cipto Mangunkusumo dan RS Kanker Dharmais sejak 9 Agt – 9 Nov 2023. Sampel darah IL-6, PCT dan CRP diambil sebelum mulai kemoterapi dan dalam 24 jam saat mulai mengalami neutropenia berat serta kultur darah aerob volume 40cc dalam 24 jam sejak neutropenia berat. Performa diagnostik delta IL-6, delta PCT, dan delta CRP lemah dan tidak berbeda bermakna sebagai penanda bakteremia dengan AUC 0,6703 (IK95% 0,507-0,833); 0,6821 (IK 95% 0,521-0,844); dan 0,694 (IK 95% 0,532-0,856) serta nilai p=0,9681. Prevalensi bakteremia pada studi ini 64,44% (29/45) dengan bakteri gram positif 77,42% (24/31) dan yang terbanyak ialah Staphylococcus Epidermidis 41,67% (10/24). Walaupun performa diagnostik ketiganya lemah, delta CRP dapat dipertimbangkan digunakan dengan mempertimbangkan biaya dan ketersediaan reagen.

IL-6, PCT, and CRP test were often performed in acute leukemia patients with febrile neutropenia as early markers of bacteremia. Our objective was to determine the diagnostic performance of delta IL-6, delta PCT, and delta CRP as early markers of bacteremia in adult acute leukemia patients with severe neutropenia during standard/aggressive chemotherapy. This research was conducted using cross-sectional design by taking all of adult acute leukemia patients with severe neutropenia during aggressive chemotherapy at Cipto Mangunkusumo Hospital and Dharmais Hospital from 9 Aug – 9 Nov 2023. IL-6, PCT and CRP blood samples were taken before starting chemotherapy and within 24 hours of starting to experience severe neutropenia as well as blood cultures aerobic volume 40cc. The diagnostic performance of delta IL-6, delta PCT, and delta CRP were weak and didn’t differ significantly as early markers of bacteremia (p = 0.968) with an AUC of 0.6703 (95% CI 0.507-0.833); 0.6821 (95% CI 0.521-0.844); and 0.694 (95% CI 0.532-0.856). Bacteremia was found in 64.44% (29/45), mostly gram-positive bacteria (77.42%) and Staphylococcus epidermidis 41.67%. Although the diagnostic performance of all three markers were weak, delta CRP can be considered as an early marker regarding the cost and availability of reagents."
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2024
T-pdf
UI - Tesis Membership  Universitas Indonesia Library