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"The purification of recombinant proteins is an important stage in biopharmaceutical research. A commonly used technique is immobilized metal affinity chromatography (IMAC). One of the main advantages of this type of chromatography is that the column can easily be regenerated for subsequent purification work. The mechanism of IMAC is based on bonding between metal ions immobilized on a matrix with a specific amino acid. Because of the strong interactions of the electron donor group on the imidazole ring, histidine is often used in the IMAC purification system. Two types of commercial IMAC resin use a nitrilotriacetic acid (NTA) matrix: a nickel-based (Ni-NTA) and cobalt-based (Co-NTA), better known as TALON. This study was aim to investigate the effect of the metal ions Ni2+ and Co2+ to purify recombinant human erythropoietin (rhEPO) expressed in yeast system Pichia pastoris. The results indicated that both Ni-NTA and Co-TALON gave almost the same level of protein purity; however, Ni-NTA has a higher binding affinity than Co-TALON might be due to the higher stability complex of Ni+. The average amount of protein bound by Ni-NTA and Co-TALON was 183.5 and 38.7 μg/mL, respectively."

"Biosorpsi Ion Timah Hitam (II) Menggunakan Sansevieria trifasciata Prain. Sansevieria trifasciata (yang dikenal sebagai tumbuhan ular atau lidah mertua) diduga dapat menjadi biosorben logam berat. Konsentrasi ion logam Pb (II) sebelum dan sesudah biosorpsi diukur dengan spektrofotometer serapan atom (SSA). Optimasi kemampuan S. trifasciata sebagai biosorben dilakukan menggunakan metode respon surface dengan variasi pada massa tumbuhan, pH larutan, waktu kontak, dan suhu. Kondisi biosorpsi optimal diperoleh pada pH 7, waktu 240 menit dan berat S. trifasciata 1.5 g dengan kapasitas biosorpsi Pb (II) sebesar 0.725 mg/g. Analisis isoterm adsorpsi menunjukkan biosorpsi mengikuti model isotherm Freundlich. Spektrum FTIR daun lidah mertua setelah diperlakukan larutan timah hitam (II) memperlihatkan intensitas puncak pada daerah 2130/cm. Mikrograf daun lidah mertua memperlihatkan struktur berpori dengan ukuran yang tidak seragam. Penyerapan Pb (II) pada serbuk daun S. trifasciata diketahui melalui analisis SEM EDX. Berdasarkan hasil penelitian, dapat disimpulkan bahwa S. trifasciata dapat digunakan sebagai biosorben untuk menghilangkan kontaminasi ion Pb.

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Sansevieria trifasciata (also called snake plant or mother in law?s tongue) is predicted to act as a heavy metal biosorbent. S. trifasciata was optimized as a biosorbent by using the response surface method with varying weights of S. trifasciata, pH of Pb (II) solutions, contact times, and temperatures. The ion concentration before and after biosorption was measured with an atomic absorption spectrophotometer (AAS). The optimum biosorption conditions were pH 7, 240 min contact time, and 1.5 g biosorbent with biosorption capacity of Pb (II) ions 0.725 mg/g. The biosorption isotherm analysis showed that the biosorption is consistent with the Freundlich isotherm model. The peak intensity of the FTIR spectrum of S. trifasciata after treatment with Pb (II) was around 2130/cm. The S. trifasciata micrograph showed a porous structure with non-uniform pore sizes. The biosorption of Pb (II) ions on powdered S. trifasciata leaves was found with the SEM EDX analysis. It is concluded from this research that S. trifasciata can be used as a biosorbent to remove Pb ion contamination."

"Biosorpsi Ion Timah Hitam (II) Menggunakan Sansevieria trifasciata Prain. Sansevieria trifasciata (yang dikenal sebagai tumbuhan ular atau lidah mertua) diduga dapat menjadi biosorben logam berat. Konsentrasi ion logam Pb (II) sebelum dan sesudah biosorpsi diukur dengan spektrofotometer serapan atom (SSA). Optimasi kemampuan S. trifasciata sebagai biosorben dilakukan menggunakan metode respon surface dengan variasi pada massa tumbuhan, pH larutan, waktu kontak, dan suhu. Kondisi biosorpsi optimal diperoleh pada pH 7, waktu 240 menit dan berat S. trifasciata 1.5 g dengan kapasitas biosorpsi Pb (II) sebesar 0.725 mg/g. Analisis isoterm adsorpsi menunjukkan biosorpsi mengikuti model isotherm Freundlich. Spektrum FTIR daun lidah mertua setelah diperlakukan larutan timah hitam (II) memperlihatkan intensitas puncak pada daerah 2130/cm. Mikrograf daun lidah mertua memperlihatkan struktur berpori dengan ukuran yang tidak seragam. Penyerapan Pb (II) pada serbuk daun S. trifasciata diketahui melalui analisis SEM EDX. Berdasarkan hasil penelitian, dapat disimpulkan bahwa S. trifasciata dapat digunakan sebagai biosorben untuk menghilangkan kontaminasi ion Pb.

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Sansevieria trifasciata (also called snake plant or mother in law?s tongue) is predicted to act as a heavy metal biosorbent. S. trifasciata was optimized as a biosorbent by using the response surface method with varying weights of S. trifasciata, pH of Pb (II) solutions, contact times, and temperatures. The ion concentration before and after biosorption was measured with an atomic absorption spectrophotometer (AAS). The optimum biosorption conditions were pH 7, 240 min contact time, and 1.5 g biosorbent with biosorption capacity of Pb (II) ions 0.725 mg/g. The biosorption isotherm analysis showed that the biosorption is consistent with the Freundlich isotherm model. The peak intensity of the FTIR spectrum of S. trifasciata after treatment with Pb (II) was around 2130/cm. The S. trifasciata micrograph showed a porous structure with non-uniform pore sizes. The biosorption of Pb (II) ions on powdered S. trifasciata leaves was found with the SEM EDX analysis. It is concluded from this research that S. trifasciata can be used as a biosorbent to remove Pb ion contamination."