Hasil Pencarian  ::  Simpan CSV :: Kembali

"Bacterial lipopolysaccharide (LPS) is impacted in the etiology of inflammatory periodontal disease. Aside from immunopathologic reactions which may be involved in the pathogenesis of the disease, the possibility exist that direct cytotoxic effect on cultured human gingival fibroblasts may be equally destructive. The expression of P53 protein can be one of markers to examine the state of impaired DNA. The purpose of this study was to investigate the effect of LPS toward expression of P53 protein on cultured human gingival fibroblasts. Cultured human gingival fibroblasts were exposed to LPS in concentrations of 50 and 200 ug/ml and untreated medium for a period of 24 and 48 hours. Cells were harvested and prepared for immunohistochemical evaluation. After exposure for 24 and 48 hours, the fraction of P53-positive cells was 81.7% in case of 50 ug/ml LPS, and 88.8% in case of 200 ug/ml LPS. After exposure for 48 hours, the fraction of P53-positive cells was 32.2% in case of 50 ug/ml LPS, and 21.1% in case of 200 ug/ml LPS. None of untreated group showed p53-positive cells. Up-regulation of p53 protein during the initial logarithmic phase of growth may be a consequence of on-going DNA damage."

"Gingivitis is an inflammatory condition of gingival tissue and caused by the accumulation of dental plaque. Green tea (Camellia sinensis) is a plant matter that has been shown to inhibit the growth of acidogenic bacteria and the formation of dental plaque. This study aimed to evaluate the effects of green tea extract on pH and volume of saliva of gingivitis patients. The subjects were 30 male gingivitis patients, 18-25 years old. The subjects were divided into 3 groups according to mouthrinse used (0.25% and 0.5% green tea extract, and 0.1% hexetidine as positive control). The subjects were instructed to gargle during five consecutive days in the morning and at night with 5 ml of the mouthrinse for 30 seconds. The data were taken in the beginning and at the sixth day. Data for salivary volume and pH before and after gargle were compared using MANOVA (p<0.05). The results showed significant differences in salivary pH, suggesting that green tea extract can increase the salivary pH of gingivitis patients, and that the 0.5% concentration was the most influential."