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Ditemukan 2 dokumen yang sesuai dengan query
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Andri Wardiana
Purification and carbohydrate analysis of recombinant human erythropoietin expressed in yeast system pichia pastoris
" ABSTRACT
For clinical purposes, pure protein and identification of carbohydrate structure from recombinant erythropoietin are needed. Purification was done by Immobilized Metal Affinity Chromatography (IMAC) column charged with Ni2+(His-Trap affinity chromatography) and continued with gel filtration chromatogram phy column to get purer protein. The carbohydrate group which is o ligosaccharide from the resulting pure protein then can be recognized by using N- and O-glycosidase. Pure oligosaccharide was hydrolyzed to produce various monosaccharide through incubation with 4 ... "
[Direktorat Riset dan Pengabdian Masyarakat UI;Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi;Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi], 2011
J-Pdf
Artikel Jurnal  Universitas Indonesia Library
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Yana Rubiyana
Comparison of Immobilized Metal Affinity Chromatography Ni-NTA and Co-TALON for the Purification of Recombinant Human Erythropoietin
" The purification of recombinant proteins is an important stage in biopharmaceutical research. A commonly used technique is immobilized metal affinity chromatography (IMAC). One of the main advantages of this type of chromatography is that the column can easily be regenerated for subsequent purification work. The mechanism of IMAC is based on bonding between metal ions immobilized on a matrix with a specific amino acid. Because of the strong interactions of the electron donor group on ... "
Jakarta: Indonesian Institute of Science (LIPI). Research Center for Biotechnology, 2015
AJ-Pdf
Artikel Jurnal  Universitas Indonesia Library