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"Indonesian Journal of Dentistry 2006; Edisi Khusus KPPIKG XIV; 397-399The purpose of this study was to determine the difference between intercellular adhesion molecule-1 (ICAM-1) cytokine content in gingival crevicular fluid (GCF) on healthy periodontal and chronic periodontitis. It was also determine the treatment effects of hydrogen peroxide as subgingival irrigation in chronic periodontitis. Twenty participants were examined in this study. Gingival fluid collection method was carry out with strip paper technique and analyzed with enzyme-linked immunosorbent assay (ELISA). The result of the study showed that there was a significant difference of ICAM-1 on healthy site and periodontitis site, and there was a significant change of ICAM-1 content after treatment (p<0.05). We concluded that ICAM-1 in GCF is a sensitive indicator to differentiate healthy sites from those with periodontitis."

"Latar belakang: Periodontitis merupakan interaksi kompleks antara sitokin pro-inflamasi dan sitokin anti-inflamasi. Sitokin anti-inflamasi seperti IL-4 pada cairan krevikular gingiva (CKG) dapat menjadi biomarker penyakit periodontal.Tujuan Penelitian: Menganalisis perbedaan kadar IL-4 dalam CKG penderita periodontitis kronis dan periodontitis agresif.Metode dan Bahan: Sampel CKG dikumpulkan dari pasien normal (n=21), periodontitis kronis (n=38) dan periodontitis agresif (n=35) dan dilakukan analisis kadar IL-4 dengan menggunakan ELISA.Hasil: Terdapat perbedaan kadar IL-4 antara periodontitis kronis dengan normal dan antara periodontitis agresif dengan normal (p=0,000). Tidak ada perbedaan kadar IL-4 (p=0,729) antara periodontitis kronis (14,73±0,32 pg/ml) periodontitis agresif (20,58±23,00 pg/ml).Kesimpulan: Kadar IL-4 menurun pada kondisi inflamasi, baik pada periodontitis kronis ataupun periodontitis agresif.

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Background: Periodontitis is a complex interaction between pro-inflammatory and anti-inflammatory cytokine. IL-4 is an anti-inflammatory cytokine in GCF that can be used as a periodontal biomarker.

Objectives: To compare level of IL-4 in GCF of chronic periodontitis and aggressive periodontitis.

Material and Methods: GCF sample were collected from normal (n=21), periodontitis chronic (n=38) and aggressive periodontitis (n=35) group and analysis of level IL-4 was performed with ELISA.

Results: There was a difference level of IL-4 between chronic periodontitis with normal and between aggressive periodontitis with normal (p=0,000). There was no difference level of IL-4 (p=0,729) between chronic periodontitis (14,73±0,32 pg/ml) and aggressive periodontitits (20,58±23,00 pg/ml).

Kesimpulan: Level of IL-4 was decreased in inflammation condition, either chronic periodontitis or aggressive periodontitis."

"Latar Belakang: Periodontitis merupakan faktor risiko terjadinya Penyakit Jantung Koroner (PJK). Interleukin-1β merupakan sitokin pro-inflamasi utama yang dapat ditemukan pada kedua penyakit ini. Tujuan: Menganalisis hubungan kadar interleukin-1β dalam cairan sulkus gingiva (CSG) penderita PJK dan non PJK dengan status periodontal. Metode: Pemeriksaan klinis 40 subjek PJK dan 40 subjek non PJK, pemeriksaan laboratorium kadar Interleukin-1β dengan ELISA. Hasil : Tidak terdapat perbedaan bermakna Interleukin-1β antara penderita PJK dan non PJK (p>0,05); tidak terdapat perbedaan bermakna antara kadar Interleukin-1β dengan status periodontal penderita PJK dan non- PJK (p>0,05). Kesimpulan: Kadar Interleukin-1β penderita PJK tidak memiliki hubungan dengan status periodontal.

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Background: Periodontitis is a risk factor for coronary heart disease. Interleukin-1β as a pro-inflammatory main cytokine, can be found in this both diseases.

Objective: To analyze the relationship of interleukin-1β levels in CSG CHD and non-CHD patients with periodontal status.

Methods: Clinical Examination for 40 Subject CHD and 40 controls was checked, laboratory test for measured the levels of Interleukin-1β with ELISA.

Results: There were no significant differences between patients Interleukin-1β CHD and non-CHD (p>0.05); there is no significant difference between the levels of Interleukin-1β with periodontal status CHD and control patients (p>0.05).

Conclusions: Levels of Interleukin-1β of CHD patients do not have a relationships with periodontal status."

"Dinamika periostin, protein matriks ekstraseluler jaringan ikat yang berperan sebagai regulator homeostasis struktural dan fungsional, ditemukan dalam cairan krevikuler gingiva (CKG) saat kondisi inflamasi maupun sehat. Pada kasus borderline, pemeriksaan secara biomolekuler dapat membantu meminimalisasi keparahan penyakit, risiko dan kerugian pasien dalam perawatan. Penelitian ini bertujuan mendapatkan perbedaan tingkat periostin sebelum dan sesudah terapi skeling dan penghalusan akar gigi (SPA) pada pasien periodontitis stage II grade A, serta hubungan antara tingkat periostin dengan kedalaman poket periodontal - PPD dan perdarahan saat probing - BOP. Sampel CKG dari enam subjek sehat [BOP (-)] dan 25 pasien periodontitis stage II grade A [PPD 4 - 5 mm, BOP (+)] usia 26 - 55 tahun di RSKGM FKG UI, Jakarta, diambil saat baseline (D0), dan dilanjutkan untuk kelompok periodontitis saat satu minggu (D1), dua minggu (D2), dan empat minggu (D3) pascaterapi SPA menggunakan paper point. Dilakukan juga pengukuran PPD dan BOP saat D0 dan D3. Tingkat periostin diamati menggunakan metode RT-PCR kuantitatif dalam 45 siklus. Nilai p<0,05 dinyatakan signifikan. Saat inflamasi, tingkat periostin ditemukan menurun, namun setelah terapi SPA, periostin meningkat dalam satu minggu (p<0,05), dua minggu (p<0,05), hingga empat minggu (p<0,05), sejalan dengan temuan klinis perbaikan PPD dan BOP. Perubahan tingkat periostin mengkonfirmasi penyembuhan jaringan pascaterapi SPA pada kasus periodontitis stage II grade A.

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The dynamics of periostin, a connective tissue extracellular matrix protein that acts as a regulator of structural and functional homeostasis, is detected in gingival crevicular fluid (GCF) during both inflammatory and healthy conditions. In borderline cases, biomolecular examinations can help minimize the severity of the disease, the risk, and the loss of patients in treatment. The aim of this study was to obtain differences in the levels of periostin before and after treatment of scaling and root planing (SRP) in patients with stage II grade A periodontitis, as well as its relationship with the depth of the periodontal pocket - PPD and bleeding on probing - BOP. Samples from GCF of six healthy subjects [BOP (-)] and 25 stage II grade A periodontitis patients [PPD 4-5 mm, BOP (+)] aged 26 - 55 years at RSKGM FKG UI, Jakarta, were taken at baseline (D0), and continued for the periodontitis group at one week (D1), two weeks (D2), and four weeks (D3) after SRP treatment by using paper points. PPD and BOP measurements were also taken at D0 and D3. Periostin levels were observed using qRT-PCR methods in 45 cycles. A value of p <0.05 was stated as significant. During inflammation, the level of periostin was found to decrease, but after SRP therapy, periostin increased in one week (p <0.05), two weeks (p <0.05), up to four weeks (p <0.05), in line with the clinical finding improvement of PPD and BOP. Periostin level changes confirmed healing of periodontal tissue after SRP therapy in stage II grade A periodontitis cases."