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Titaporn Chaisin
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ABSTRACT
Cyclodextrin glycosyltransferase (CGTase) catalyzes an intermolecular transglycosylation reaction to produce functional oligosaccharides or glycosides, which can be used in several industries. In this study, the p19bBC recombinant cells containing CGTase gene from Bacillus circulans A11 were used for synthesizing CGTase. The maximum expression was obtained when the p19bBC cells were cultured at 37oC for 24 h with 0.2 mM IPTG. The recombinant CGTase was purified up to 6-fold by 5% (w/v) starch adsorption and the ...
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Pathum Thani: Thammasat University, 2018
670 STA 23:2 (2018)
Artikel Jurnal Universitas Indonesia Library