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Yosi Kusuma Eriwati

Studi karakteristik mikrostruktur dan sifat dentin manusia terhadap panas sebagai modifikasi permukaan dentin

"ABSTRAK

Telah dilakukan penelitian untuk mengetahui efek perlakuan panas melalui

pemanasan fumace dan radiasi Nd:YAG Iaser terhadap perubahan mikrostruktur,

kekerasan mikro dan morfologi permukaan dentin manusia sebagai modifikasi

permukaan dentin. Dengan menggunakan sampel dentin dari gigi impaksi molar

3, perubahan energi panas yang berhubungan dengan transformasi fisik dan

khemis sebagai fungsi suhu menunjukkan kurva endotermik pada 111,62 °C

yang mengindikasikan kristalinitas yang Iebih rendah pada dentin dengan kristal

yang tidak homogen. Hasil DSC dentin menunjukkan kurva endotermik pada 40

°C - 284 "C, dan puncak eksotermik teriihat pada 500 °C. Total kehilangan berat

dentin dengan analisa TG adalah 21 ,26% setelah pemanasan 900 °C. Hasil XRD

pada suhu di atas 750 °C memperlihatkan puncak difraksi dari hidroksiapatit yang

meningkat dengan intensitas yang Iebih tinggi dengan munculnya puncak

Whitlockite. Perhitungan ukuran kristalit dentin yang bertambah besar

menunjukan adanya pertumbuhan kristalit akibat perlakuan panas sehingga

kristalinitas dentin berubah mendekati kristalinitas hidroksiapatit email. Nilai

kekerasan mikro Vickers jug meningkat akibat energi radiasi laser sesuai

dengan meningkatnya intensitas puncak difraksinya. Setelah radiasi Nd:YAG

laser terbentuk kawah dengan permukaan dentin yang meleleh dan mengalami

rekristalisasi. Jadi perilaku dentin manusia memperlihatkan perubahan fisik dan

khemis terhadap kenaikan suhu pemanasan dan energi radiasi laser yang tinggi.

Efek panas menyebabkan perubahan mikrostruktur dan sifat kekerasan yang

meningkat mendekati struktur dan kekerasan email.

Abstract

The purpose of this study was to investigate the effects of heat treatment

and Nd:YAG laser irradiation on the microstructure, microhardness and

morphological changes of human dentin surfaces for alternative dentin surface

modification. The results on the DSC of dentin show a large endothermic curve at

40°C to 284°C, likewise the DTA endothermic peak at 111,62 °C which

represent a material of less crystallinity and inhomogenous crystals. An

exothermic peak was also shown at 500ºC by DSC. The total loss of dentin

weight by TG analysis was 21.26% after heated to 900°C. XRD investigation

revealed that at higher temperature (above 750 °C) the amount of diffraction

peaks of hydroxyapatite were higher and more intense with the development of

Whitlockite. The crystallite size were also higher showing crystal growth upon

heat-treatment. After Nd:YAG laser-treated, dentin with relatively low

crystallinities obtains a structure which comes to resemble the crystalline

structure of enamel hydroxyapatite. VIckers micro-hardness property on lased

dentin surfaces showed increasing values of all laser exposures that were

associated with the increase intensity of peak diffraction. SEM observations on

Nd:YAG laser irradiation on dentin surface resulted in creater formation at higher

energy output, as well as surface melting, recrystallized and glazed surfaces. The

thermal behavior of human dentin shows physical and chemical changes with

higher temperature and higher energy output of Nd:YAG laser treatment.

Thermal effects caused microstructure and morphological changes on dentin

surfaces with increased microhardness surface properties which resemble the

microstructure and hardness of enamel."

2003

D1224

UI - Disertasi Membership Universitas Indonesia Library

Aryo Megantoro

Efek aplikasi biodentinetm terhadap remineralisasi affected dentin setelah partial caries excavation in vivo = The Effect of biodentinetm application in affected dentin remineralization after partial caries excavation in vivo

"Latar belakang: Perawatan lesi karies dalam masih banyak dilakukan dengan pembuangan seluruh jaringan karies karena kekhawatiran proses karies berlanjut apabila jaringan karies ditinggalkan. Perawatan tersebut berisiko tinggi kematian pulpa. Dengan kemajuan ilmu teknologi kedokteran gigi, dapat dilakukan perawatan secara minimal invasif dengan meninggalkan jaringan karies.

Tujuan: Mengevaluasi perbandingan remineralisasi affected dentin dengan meninggalkan sebagian atau mengangkat seluruh infected dentin pada lesi karies dalam.

Metode: Dua kelompok, dengan meninggalkan atau mengangkat infected dentin. Pada kedua kelompok diaplikasikan BiodentineTM dan dievaluasi setelah 4 minggu.

Hasil: Kelompok I menunjukkan terjadi remineralisasi, tetapi belum tampak di radiograf. Kelompok II menunjukkan adanya remineralisasi. Perbandingan remineralisasi antara kedua kelompok tidak signifikan

Kesimpulan: Remineralisasi affected dentin dapat terjadi dengan meninggalkan infected dentin ataupun mengangkat seluruhnya.

Background: The treatment of deep carious lession still done with complete carious removal, risking the pulp to necrosis. With the upgrade in dental science and technology, nowadays dentist can provide minimal invasive carious removal by leaving partly carious lession.
Objective: To evaluate the remineralization in affected dentin by leaving partly infected dentin or remove all infected dentin.
Methods: Two groups, leaving partly or remove all infected dentin. Both group applied with BiodentineTM and evaluated after four weeks.
Result: First gruop showed remineralization, but radiographically insignificant. Second group showed remineralization. The comparation between two groups insignificant.
Conclusion: Affected dentin remineralization can happened, either leaving the infected dentin or remove all of them."

Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2017

SP-Pdf

UI - Tugas Akhir Universitas Indonesia Library

Napitupulu, Elizhabet

Pengaruh Penambahan Fluor dalam Sistem PILP terhadap Perubahan Topografi Dinding Tubulus Dentin dan Persentase Fluoroapatit = Effect of Fluorine Addition in the PILP System on Topographical Changes of the Dentine Tubular Walls and the Percentage of Fluoroapatite

"Latar Belakang: Remineralisasi dentin dapat dicapai melalui beberapa metode, di

antaranya secara Guided Tissue Remineralization (GTR) dalam sistem Polymer-Induced Liquid Precursor (PILP). Remineralisasi secara GTR terbukti dapat meremineralisasi affected dentin dengan membentuk mineral intrafibrillar dan ekstrafibrillar. Melalui sistem PILP, kristal terbentuk dengan ukuran kecil sehingga remineralisasi lebih banyak terjadi secara intrafibrillar. Penambahan fluor dalam sistem PILP diharapkan dapat membentuk kristal fluoroapatit yang berukuran lebih besar dan mampu menyempurnakan remineralisasi hingga ke ekstrafibrillar. Penelitian yang ada selama ini hanya berfokus pada permukaan dentin, sedengkan belum terdapat penelitian untuk membuktikan remineralisasi dengan penambahan fluor yang terjadi pada dinding tubulus dentin. Tujuan: Mengetahui pengaruh penambahan fluor 5ppm dan 25ppm dalam sistem PILP terhadap perubahan topografi dinding tubulus dentin dan persentase fluoroapatit. Metode: Sampel blok dentin terdemineralisasi direndam pada larutan remineralisasi dengan penambahan 5ppm dan 25ppm fluor. Sampel blok dentin kemudian akan dipotong lintang menggunakan metode fraktur lalu diamati dan dianalisis menggunakan uji FE-SEM dan XRD. Hasil: Terjadi perubahan topografi pada dinding tubulus dentin setelah dilakukan remineralisasi melalui proses PILP

dengan penambahan 5ppm dan 25ppm fluor selama 14 hari yang dievaluasi secara deskriptif menggunakan FE-SEM. Hasil analisis XRD menunjukkan tidak ada perbedaan bermakna secara statistik penambahan fluor pada larutan remineralisasi PILP terhadap persentase fasa mineral fluoroapatit, namun secara substansi terjadi

peningkatan persentase fluorapatit. Kesimpulan: Penambahan fluor dalam sistem PILP berpengaruh terhadap perubahan topografi dinding tubulus dentin dan persentase

fluoroapatit.

Background: Dentine remineralization can be achieved through several methods,
including Guided Tissue Remineralization (GTR) in the Polymer-Induced Liquid
Precursor (PILP) system. GTR remineralization has been shown to remineralize
affected dentin by forming intrafibrillar and extrafibrillar minerals. Through the PILP
system, crystals are formed with small sizes so that more remineralization occurs
intrafibrillarly. The addition of fluorine in the PILP system is expected to form larger
fluoroapatite crystals and be able to complete the remineralization to extrafibrillar. Existing research so far has only focused on the dentin surface, although there has been no research to prove remineralization with the addition of fluorine that occurs in the dentine tubule walls. Objective: To determine the effect of adding 5ppm and 25ppm fluorine in the PILP system on changes in the topography of the dentinal tubule walls and the percentage of fluoroapatite. Methods: Demineralized dentine block samples were immersed in remineralization solution with the addition of 5 ppm and 25 ppm fluorine. The dentine block samples will then be cross-sectioned using the fracture method and then observed and analyzed using the FE-SEM and XRD tests. Results: Topographical changes occurred in the dentinal tubule walls after remineralization through the PILP process with the addition of 5ppm and 25ppm fluorine for 14 days which were evaluated descriptively using FE-SEM. The results of the XRD analysis showed that there was no statistically significant difference in the addition of fluorine to the percentage of fluoroapatite mineral phase in the addition of fluorine to the percentage of fluoroapatite, but in substance there was an increase in the percentage of fluorapatite. Conclusion: The addition of fluorine in the PILP system affected the topography of the dentinal tubule walls and the percentage of fluoroapatite."

Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2023

T-pdf

UI - Tesis Membership Universitas Indonesia Library

Wees Kaolinni

Potensi semen hidraulik dikalsium silikat kalsium dari cangkang telur dan silika sekam padi terhadap remineralisasi dentin: analisis in vitro = Dentin remineralization potential of eggshell calcium and rice husk silica dicalcium silicate hydraulic cement: an in vitro analysis

"Latar Belakang: Semen hidraulik C2SCS merupakan salah satu bahan bioaktif yang disintesis dari bahan alami, yaitu cangkang telur dan sekam padi. Tujuan: Mengetahui efek semen C2SCS dari cangkang telur dan sekam padi terhadap peningkatan derajat kristalinitas hidroksiapatit dentin. Metode: 24 sampel gigi dibuat kavitas buatan dan dibagi menjadi 4 kelompok. Kelompok 1 (dentin normal) sebagai kontrol, kelompok 2 dentin demineralisasi, kelompok 3 dentin demineralisasi diaplikasi semen C2SCS dengan rasio pencampuran 1:1, kelompok 4 dentin demineralisasi diaplikasi semen C2SCS rasio 1:2. Seluruh sampel disimpan dalam shaking incubator pada suhu 37° C selama 14 hari. Kemudian sampel gigi tersebut diperiksa dengan XRD dan SEM untuk melihat peningkatan jumlah kristal hidroksiapatit dentin. Hasil: Terdapat peningkatan derajat kristalinitas hidroksiapatit dentin setelah aplikasi semen C2SCS rasio 1:1 yang nilainya berbeda bermakna dengan dentin demineralisasi, dan tidak berbeda bermakna dengan dentin sehat (kontrol positif). Semen C2SCS rasio 1:1 lebih berpotensi untuk meningkatkan pembentukan kristal hidroksiapatit pada dentin demineralisasi dibanding rasio 1:2 Kesimpulan: Semen C2SCS dari cangkang telur dan sekam padi memiliki potensi untuk meremineralisasi dentin. Semen C2SCS rasio 1:1 memiliki potensi remineralisasi dentin yang lebih tinggi dibanding kelompok lainnya.

Background: C2SCS cement is one of bioactive material synthesized from natural material, such as eggshell calcium and rice husk silica. Objective: To determine the effect of C2SCS cement made from eggshell and rice husk silica to the increase of degree of cristallinity of dentin hydroxyapatite. Methods: Artificial cavity was made in 24 tooth sample, which then divided into 4 groups. Group 1 is normal dentin as the positive control group. Group 2 is demineralized dentin. Group 3 is demineralized dentin applied with C2SCS cement in mixing ratio of 1:1. Group 4 is demineralized dentin applied with C2SCS cement in mixing ratio of 1:2. All samples are stored inside a shaking incubator at a temperature of 37 C. An examination using XRD was done to all samples to see the degree of cristallinity of dentin hydroxyapatite, and a SEM image is taken to see morphology and microstructure of hydroxyapatite. Result: There is a significant increase of degree of cristallinity of dentin hydroxyapatite after C2SCS cement application with 1:1 ratio compared to demineralized dentin, and insignificant difference with normal dentin. C2SCS cement in 1:1 ratio has more potential to increase the formation of hydroxyapatite crystal compared to 1:2 ratio. Conclusion: C2SCS cement made from eggshell and rice husk silica has the potential to remineralize dentin. C2SCS cement in 1:1 ratio has more remineralization potential compared to other groups."

Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2022

SP-pdf

UI - Tugas Akhir Universitas Indonesia Library

Nevada Permata Anvini

Efektivitas aplikasi silver diamine fluoride dan laser CO2 dalam menurunkan skor hipersensitivitas dentin = Effectiveness of silver diamine fluoride and CO2 laser in reducing dentin hypersensitivity score

"Latar belakang: Silver Diamine Fluoride dan Laser CO2 merupakan alternatif perawatan yang dapat dipilih untuk menangani hipersensivitas denton.

Tujuan: Mengetahui efektivitas penggunaan Silver Diamine Fluoride dan Laser CO2 dalam menurunkan skor hipersensitivitas dentin.

Metode: Penelitian ini menggunakan desain single blind, split-mouth randomized controlled trial yang melibatkan 16 subjek dengan keluhan gigi sensitif dan memiliki minimal dua gigi yang positif terhadap uji evaporatif atau uji thermal. Subjek akan diaplikasikan Silver Diamine Fluoride dan Laser CO2. Pengukuran hipersensitivitas dentin dilakukan dengan stimulus evaporatif dan termal dan DIAGNOdent pasca aplikasi, 7 hari dan 14 hari setelah aplikasi.

Hasil: Terjadi penurunan skor Hipersensitivitas dan DIAGNOdent yang berbeda bermakna terhadap data baseline segera pasca aplikasi, 7 hari dan 14 hari pasca aplikasi p=0,000 untuk stimulus evaporatif, termal dan DIAGNOdent . Tetapi tidak terdapat perbedaan bermakna secara statistik antara aplikasi Silver Diamine Fluoride dan aplikasi Silver Diamine Fluoride dan Laser CO2 pada setiap pengukuran.

Kesimpulan: Penggunaan Silver Diamine Fluoride dan Laser CO2 efektif dalam menurunkan skor hipersensitivitas dentin.

Background: Silver Diamine fluoride and CO2 Laser are a new alternative treatment for dentin hypersensitivity.
Objective: To evaluate the efficacy of Silver Diamine Fluoride and CO2 Laser in reducing dentin hypersensitivity score.
Methods: The study used a single blind, split mouth design randomized controlled trial involving 16 subjects with symptoms of sensitive teeth and have at least two hypersensitive teeth which are positive to evaporative or thermal test. The subject teeth surface will be applied Silver diamine fluoride and CO2 Laser will be applied to the subject teeth surface.
Results: This clinical study demonstrated that the application of Silver Diamine Fluoride and CO2 Laser reduce dentin hypersensitivity significantly in each measurement p 0,000 for evaporative and thermal stimulus. But there is no statistically significant differences between the two application.
Conclusion: The usage of Silver Diamine Fluoride and CO2 Laser are effective to reduce the degree of dentin hypersensitivity. "

Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2016

S-Pdf

UI - Skripsi Membership Universitas Indonesia Library

Itja Risanti

Efek Klorheksidin terhadap pengurangan degradasi kekuatan ikat geser resin komposit-Dentin (eksperimen laboratorik) = Chlorhexidine effect against degradation of composite resin-dentin shear bond strength (laboratory experiment)

"Ketahanan ikatan resin komposit-dentin merupakan salah satu penentu keberhasilan restorasi resin komposit. Tujuan penelitian ini adalah menganalisis efek klorheksidin terhadap degradasi kekuatan ikat resin kompositdentin.

Metode: Dua puluh empat sampel dentin yang diambil dari mahkota gigi premolar, dibagi menjadi tiga kelompok yang diberikan perlakuan berbeda. Kelompok I diberi perlakuan bahan bonding tanpa klorheksidin, kelompok II diberi perlakuan klorheksidin dan bonding, kelompok III diberi perlakuan bonding mengandung klorheksidin, pada tiap kelompok dibagi menjadi 2 sub-kelompok yaitu kelompok tanpa direndam dan kelompok yang direndam NaOCl 10% selama satu jam, sehingga didapat enam sub-kelompok. Kemudian seluruh kelompok di ukur kekuatan ikat gesernya menggunakan Universal Testing Machine. Satu sampel dari setiap sub-kelompok dilakukan Scanning Electron Microscope (SEM). Data dianalisa statistik dengan uji hipotesis Kruskal Wallis yang dilanjutkan dengan uji Mann-Whitney.

Hasil: rerata kekuatan ikat geser sebelum perendaman NaOCl 10% tertinggi kelompok I sedangkan rerata kekuatan ikat geser setelah perendaman NaOCl 10% tertinggi pada kelompok III. Terdapat perbedaan bermakna antara kelompok I terhadap kelompok II dan antara kelompok II terhadap kelompok III.

Kesimpulan: Klorheksidin mempunyai efek terhadap pengurangan degradasi kekuatan ikat geser resin komposit-dentin.

Resilience of composite resin-dentin bonding known as one of success composite resin restoration determinants. The purpose of this study was to analyze the effect of chlorhexidine on reducing the degradation of composite resin-dentin shear bond strength.
Methods: Twenty-four premolar crowns were divided into three groups then given different treatments. Group I was treated material bonding without chlorhexidine, group II was treated with chlorhexidine and bonding, group III was treated with chlorhexidine-contained bonding. Each group was divided into two sub-groups: the group without immersion of NaOCl 10% and the group with immersion of NaOCl 10% for one hour, then it were obtained six sub-groups. After twenty-four hours, shear bond strengths measured using Universal Testing Machine. A sample of each group was photographed with Scanning Electron Microscope (SEM). Statistical analysis was done by Kruskal Wallis test, then followed by Mann Whitney test to determine significance between groups.
Results: The mean value of shear bond strength before immersion of NaOCl 10% was highest on Group I, while the mean value of shear bond strength after immersion of NaOCl 10% was highest on Group III. There are significant differences between Group I with Group II and between Group II with Group III.
Conclusion: Chlorhexidine have an effect on reducing the degradation of shear bond strength of resin-dentin bonding."

Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2012

T31955

UI - Tesis Open Universitas Indonesia Library

Albert

Pengaruh serotipe c streptococcus mutans terhadap tingkat keasaman plak dan saliva pada penderita resesi gingiva dengan hipersensitif dentin (analisis menggunakan real time PCR) = Effect of serotype c streptococcus mutans for plaque and saliva acidity levels in gingival recession with dentin hypersensitivity (analysis using real time PCR)

"[ABSTRAK

Latar Belakang: Hipersensitif dentin dipengaruhi oleh akumulasi plak pada

permukaan gigi dan penetrasi bakteri pada tubulus dentin. Tujuan: Menganalisis

proporsi serotipe c Streptococcus mutans dan tingkat keasaman di dalam plak

dan saliva penderita resesi gingiva yang hipersensitif dentin dengan penderita

resesi gingiva yang non hipersensitif. Metode: Tiga puluh enam sampel plak dan

saliva dibagi dalam dua kelompok yaitu kelompok hipersensitif dan non

hipersensitif. Dilakukan ekstrak DNA sampel, pengukuran pH sampel dan

evaluasi amplifikasi serotipe c Streptococcus mutans dengan alat Real Time

PCR. Hasil: Proporsi serotipe c Streptococcus mutans di dalam plak dan

saliva tidak berbeda pada penderita resesi gingiva dengan hipersensitif

dentin maupun non hipersensitif, Kesimpulan: Proporsi serotipe c

Streptococcus mutans plak dan saliva tidak mempengaruhi hipersensitif

dentin.

ABSTRACT

Background: dentin hypersensitivity is affected by the accumulation of plaque

on the tooth surface and penetration of bacteria in the dentinal tubules.

Objective: To analyze the proportion of serotype c Streptococcus mutans and the

level of acidity in plaque and saliva of patients with hypersensitive dentin and

non hypersensitive. Methods: Thirty-six plaque and saliva samples were divided

into two groups: the hypersensitive and non-hypersensitive. Extract the sample

DNA, measure the acidity levels and evaluate serotype c Streptococcus mutans

amplification with Real Time PCR. Results: The proportion of serotype c

Streptococcus mutans in plaque and saliva is not significantly different in the

patients with gingival recession both hypersensitive and non-hypersensitive,

Conclusions: The proportion of serotype c Streptococcus mutans in plaque and

saliva are equally well both in hypersensitive and non hypersensitive cases.;Background: dentin hypersensitivity is affected by the accumulation of plaque