Ditemukan 1 dokumen yang sesuai dengan query
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Microglia were isolated from mixed primary cell cultures of the cerebral cortex from 3 day old male Wistar rats. The mechanically dissociated cells were plated in a flask at a density of 107per 300 cm2 and maintained at 30°C in a 10% CO2/90% air atmosphere. After 10-14 days in culture, floating and weakly attached cells on the mixed primary cultured cell layer were isolated by gentle shaking of the flask for 3-5 min. The resulting ...
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Journal of Dentistry Indonesia, 2005
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Artikel Jurnal Universitas Indonesia Library