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Hasil Pencarian

Ditemukan 6 dokumen yang sesuai dengan query
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Rafasyah Syafira Darsono
"Bawang merah (Allium ascalonicum L.) adalah komoditas sayuran dengan ancaman penyakit hawar daun bakteri yang disebabkan oleh Pantoea sp. dalam produksinya. Pemanfaatan agen biokontrol dari bakteri yang berasosiasi dengan akar tanaman berpotensi tinggi menghasilkan mekanisme penghambatan patogen pada tanaman. Penelitian ini bertujuan untuk memperoleh isolat bakteri agen biokontrol yang mampu menghambat pertumbuhan patogen Pantoea sp., mempelajari karakterisasi dan mekanisme aktivitas agen biokontrol dalam penghambatan patogen Pantoea sp., serta mengidentifikasi isolat bakteri agen biokontrol. Kultur bakteri kandidat agen biokontrol diisolasi dari tanah perakaran tanaman bawang merah di lahan pertanian bawang merah di Cirebon dan Majalengka, Jawa Barat, serta Brebes, Jawa Tengah. Pengujian aktivitas meliputi uji antagonisme dan karakterisasi aktivitas biokontrol (produksi enzim protease, kitinase, selulase, siderofor, hidrogen sianida, ammonia, peroksidase dan aktivitas katalase) secara in vitro. Identifikasi isolat bakteri agen biokontrol dengan dilakukan gen 16S rRNA dan dilanjutkan dengan pembuatan rekonstruksi pohon filogenetik. Berdasarkan hasil penelitian, diperoleh 5 isolat rizobakteri (I-8, L1-9, L1-12, BM-L3(2), dan BM-L3(3)) yang memiliki kemampuan antagonis terhadap patogen Pantoea sp. penyebab penyakit hawar daun bakteri pada bawang merah. Kelima isolat tersebut menunjukkan hasil positif pada uji aktivitas enzim protease, kitinase, selulase, siderofor, hidrogen sianida, ammonia, peroksidase dan aktivitas katalase. Hanya isolat BM-L3(2) yang tidak memiliki kemampuan dalam produksi enzim kitinase. Isolat BM-L3(2) dan BM-L3(3) teridentifikasi sebagai Brevibacillus schisleri dan Bacillus sp., sedangkan isolat I-8, L1-9, dan L1-12 teridentifikasi sebagai Streptomyces rochei berdasarkan sifat morfologi, analisis sekuens 16S rRNA, dan kekerabatan pada hasil rekonstruksi pohon filogeni.

Shallot (Allium ascalonicum L.) is vegetable commodity that has the threat of bacterial leaf blight caused by Pantoea sp. in its production. Utilization of biocontrol agents from bacteria associated with plant roots has high potential to produce pathogen inhibition mechanisms in plants. This research aims to obtain bacterial isolates of biocontrol agents that are able to inhibit the growth of Pantoea sp., study the characterization and mechanism activity of biocontrol agents, and identify bacterial of biocontrol agents. Bacterial of biocontrol agent candidates were isolated from root soil of shallot plants in Cirebon, Majalengka, and Brebes. The activity of the antagonism and biocontrol charaterization (enzyme production of protease, chitinase, cellulase, siderophore, HCN, ammonia, peroxidase and catalase) was tested by in vitro. Identification of biocontrol agent bacterial with the 16S rRNA gene was carried out and continued with phylogenetic tree reconstruction. Based on the results of the study, 5 rhizobacterial isolates (I-8, L1-9, L1-12, BM-L3(2), and BM-L3(3)) were obtained that have antagonism activity as biocontrol agent bacteria against the pathogen Pantoea sp. causing bacterial leaf blight in shallots. The five isolates showed positive results in the enzyme activity tests of protease, chitinase, cellulase, siderophore, hydrogen cyanide, ammonia, peroxidase, and catalase. Only isolate BM-L3 (2) does not have the ability to produce chitinase enzyme. Isolates BM-L3(2) and BM-L3(3) were identified as Brevibacillus schisleri and Bacillus sp., while isolates I-8, L1-9, and L1-12 were identified as Streptomyces rochei based on morphological characters, 16S rRNA sequence analysis, and kinship on phylogeny tree reconstruction results."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2024
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UI - Skripsi Membership  Universitas Indonesia Library
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Sharma, Hari C.
Boca Raton: CRC Press, 2009
632.96 SHA b
Buku Teks  Universitas Indonesia Library
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Wallingford: CABI, 2013
614.432 BIO
Buku Teks SO  Universitas Indonesia Library
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Oxford: Blackwell, 1992
591.53 NAT
Buku Teks  Universitas Indonesia Library
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Guy Smagghe, editor
"The book consists of multiple chapters by leading experts on the different aspects in the unique relationship between arthropods and plants, the underlying mechanisms, realized successes and failures of interactions and application for IPM, and future lines of research and perspectives. Interesting is the availability of the current genomes of different insects, mites and nematodes and different important plants and agricultural crops to bring better insights in the cross talk mechanisms and interacting players. "
Dordrecht: [, Springer], 2012
e20417369
eBooks  Universitas Indonesia Library
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"lants provide insects with a range of specific foods, such as nectar, pollen, and food bodies. In exchange, they may obtain various services from arthropods. The role of food rewards in the plant-pollinator mutualism has been covered broadly. This book addresses another category of food-mediated interactions, focusing on how plants use foods to recruit arthropod "bodyguards" as a protection against herbivores." "Many arthropods with primarily carnivorous lifestyles require plant-provided food as an indispensable part of their diet. Only recently have we started to appreciate the implications of non-prey food for plant-herbivore-carnivore interactions. Insight into this aspect of multitrophic interactions is not only crucial to our understanding of the evolution and functioning of plant-insect interactions in natural ecosystems, it also has direct implications for the use of food plants and food supplements in biological control programs.". "This edited volume provides essential reading for all researchers interested in plant-insect interactions."--BOOK JACKET.
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Cambridge, UK: Cambridge University Press, 2005
632 PLA
Buku Teks  Universitas Indonesia Library