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"Sepsis merupakan suatu penyakit sistemik yang dapat disebabkan oleh translokasi bakteri. B. animalis subsp. lactis merupakan salah satu bakteri yang berpotensi dalam mencegah translokasi bakteri. Gen grpE merupakan salah satu target spesifik dalam mendeteksi B. animalis subsp. lactis. Penelitian bertujuan untuk mengoptimasi primer dengan target gen grpE untuk kurva standar, mengetahui hubungan konsentrasi primer terhadap nilai Ct, serta mengetahui sensitivitas dan spesifisitas primer. Isolat diisolasi dari sampel feses bayi menggunakan metode fenol-kloroform. Pasangan Primer dirancang berdasarkan sekuens gen grpE B. animalis subsp. lactis (NZ_ABOT01000010.1) menggunakan program Primer3. Optimasi primer dilakukan menggunakan lima konsentrasi berbeda,yaitu 50/50 nM, 100/100 nM, 300/300 nM, 500/500 nM, dan 1.000/1.000 nM.Hasil penelitian menunjukkan bahwa pasangan primer F_HNO19_grpE dan R_HNO19_grpE dengan konsentrasi 1.000/1.000 nM menghasilkan kurva standar yang optimal dengan efisiensi dan koefisien korelasi (R2) masing-masing sebesar 99,095% dan 0,971. Berdasarkan uji sensitivitas dan spesifistas, pasangan primer F_HNO19_grpE dan R_HNO19_grpE konsentrasi 1.000/1.000 nM dapat mengamplifikasi DNA target sampai dilusi 10-5 , tetapi spesifisitasnya hanya sampai dilusi 10-2. Konsentrasi primer tidak berkorelasi terhadap nilai Ct. Pasangan primer F_HNO19_grpE dan R_HNO19_grpE dapat digunakan untuk kuantifikasi B. animalis subsp. lactis dengan kisaran nilai Ct sebesar 15,74--33,89.

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Sepsis is a systemic disease that can be caused by bacterial translocation. B. animalis subsp. lactis is one of the bacteria that has the potential to prevent the bacterial translocation. grpE gene is a specific target in the detection of B.animalis subsp. lactis. The research aims to optimize primer pair with target gene grpE for generating standard curve, to know the correlation between primer concentration and Ct value, and to know the primer sensitivity and specificity. Isolates were isolated from infant stool samples using phenol-chloroform method. Primer pair is designed based on B. animalis subsp. lactis grpE gene sequence (NZ_ABOT01000010.1) using the Primer3 program. The primer optimization is done using five different concentrations, which are 50/50 nM, 100/100 nM,300/300 nM, 500/500 nM, and 1.000/1.000 nM. The results showed that the primer pair F_HNO19_grpE and R_HNO19_grpE with 1.000/1.000 nM concentration can be used to generate an optimal standard curve with efficiency and correlation coefficient (R2) each by 99.095% and 0.971. Based on sensitivity and specificity test, primer pair F_HNO19_grpE and R_HNO19_grpE with 1.000/1.000 nM concentration can amplify DNA targets up to 10-5 dilution, but its specificity is only up to 10-2 dilution. Primer concentration and DNA samples with different concentrations were not correlated to the Ct value. F_HNO19_grpE and R_HNO19_grpE primer pair can be used for quantification of B. animalis subsp. lactis with a range of Ct values of 15.74 to 33, 89."

"Demam Berdarah Dengue (DBD) adalah penyakit tular vektor oleh virus dengue dengan vektor Aedes sp. Cempaka Putih Barat tergolong salah satu kelurahan dengan jumlah kasus DBD yang tinggi, sehingga perlu dilakukan pengendalian vektor dengan memberantas tempat berkembangbiak nyamuk Aedes sp., yaitu container luar rumah. Penelitian ini dilakukan dengan tujuan mengetahui sebaran container luar rumah dan keberadaan larva Aedes sp di RW 03 dan RW 07 Kelurahan Cempaka Putih Barat. RW tersebut dipilih karena homogenitas karakteristik pemukiman, dimana RW 03 dihuni oleh rumah besar dengan jarak rumah yang lebar dan RW 07 dihuni oleh rumah kecil, padat dengan jarak rumah yang sempit. Penelitian menggunakan desain cross sectional dan survei larva menggunakan single larva method. Di RW 03 ditemukan enam puluh container luar rumah dengan empat belas jenis container dan terbanyak ember. Larva paling banyak ditemukan di tong. Di RW 07 ditemukan 31 container luar rumah dengan sepuluh jenis container dan terbanyak kolam/akuarium. Larva paling banyak ditemukan di drum dan kaleng bekas. Dengan demikian container luar rumah lebih banyak ditemukan di RW 03 tetapi berdasarkan uji statistik didapatkan container luar rumah positif larva lebih banyak di RW 07 (25,8%) daripada di RW 03 (8,3%) dengan (p=0,024), dapat disimpulkan terdapat perbedaan bermakna keberadaan larva Aedes sp. pada container luar rumah di RW 03 dan RW 07 Kelurahan Cempaka Putih Barat.

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Dengue Hemorrhagic Fever (DHF) is a vector-borne disease by dengue virus and Aedes sp. as vector. West Cempaka Putih as one of district that the number of dengue cases are high. Therefore it is necessary to control the vector by eradicating potential breeding ground for Aedes sp. mosquitoes, container outside the house.The aim of study to identify the distribution of outdoor container and the presence of Aedes sp. larvae in RW 03 and RW 07 Cempaka Putih Barat. These was chose because homogenity of settlement characteristic that RW 03 is populated by people with big house and distance between houses are far. RW 07 is populated by people with small house and distance between houses are quite close. This study used cross sectional design and survey of larvae used single larvae method. In RW 03 was found sixty outdoor containers, fourteen types of container with most frequent container is bucket and larvae most commonly found in cans. In RW 07 was found thirty one outdoor containers, ten types of container with most frequent container is pond/aquarium and larvae most commonly found in the drums and tin cans. Thus the outdoor container is more commonly found in RW 03, but based on statistic test found that the number of containers with larvae in RW 07 (25,8%) is higher than in RW 03 (8.3%) with (p = 0.024). It can be concluded that there are significant differences in the existence of Aedes sp. larvae in the outdoor container between RW 03 and RW 07 West Cempaka Putih."