Hasil Pencarian  ::  Simpan CSV :: Kembali

"Penelitian ini telah berupaya untuk meningkatkan tekstur keju yang dibuat dari susu sapi segar, dengan menggunakan pengganti enzim rennet sebagai koagulan. Susu mengandung dua protein utama yaitu kasein dan whey. Namun bagian yang penting untuk dijadikan keju adalah kasein, di mana kasein ini yang mengalami koagulasi untuk membentuk produk keju. Koagulan yang digunakan adalah kombinasi enzim papain dan transglutaminase. Berdasarkan penelitian-penelitian sebelumnya, enzim papain bisa digunakan untuk mengkoagulasi kasein susu menjadi keju karena kemampuan proteolitiknya. Enzim transglutaminase digunakan untuk meningkatkan tekstur keju, dengan kemampuan ikatan silangnya. Proses pembuatan keju ini terdiri atas tiga variasi konsentrasi enzim papain yaitu 0,5; 1,5; dan 2;5 gram/100 ml susu untuk menentukan yield yang dihasilkan paling banyak, dan variasi kadar enzim transglutaminase sebesar 0,5; 1; 2 UE/gram protein susu atau setara dengan 0,027; 0,054; dan 0,11 gram enzim/100 ml susu. Pada sampel keju terbaik yang dihasilkan dilakukan pengujian seberapa banyak yield, pH, uji FTIR, uji proksimat, mikrostruktur keju, uji potensial zeta, dan uji profil tekstur. Yield yang dihasilkan sebesar 23,33%, dengan pH 6,3, dan nilai puncak potensial zeta 0,97. Mikrostruktur keju yang diamati menggunakan Scanning Electron Microscope dengan perbesaran menunjukkan penambahan TGase menyebabkan koagulum lebih memadat. Nilai proksimat yang didapat menandakan penambahan TGase meningkatkan kadar lemak dan karbohidrat, dan menurunkan kadar air dan protein. Profil tekstur yang didapat menandakan keju dengan TGase lebih keras dan elastis karena adanya peningkatan kandungan lemak dan ikatan silang lebih lanjut pada matriks protein oleh TGase"

"This Brief provides the current state-of-the-art on all key topics involved in GMO testing and is a source of detailed practical information for laboratories. Special focus is given to qualitative and quantitative real-time PCR analysis relevant to all areas where detection and identification rely on nucleic acid-based methods. The following topics, important for testing laboratories, are also discussed, organization of the laboratory, focusing on aspects of the quality system and methods for testing, validation and verification of methods, and measurement uncertainty. The Brief also discusses the new challenges of GMOs and novel modified organisms, using new technologies, and the possible solutions for GMO detection, including bioinformatics tools. Finally, legislation on GMOs and sources of information on GMOs are provided, which are relevant not only to testing laboratories, but to anyone interested in GMOs."

"Telah dilakukan penelitian untuk mengidentifikasi efek krioprotektif madu lengkeng (Dimocarpus longan) terhadap integritas struktur folikel preantral dan profil ekspresi protein apoptosis jalur intrinsik. Sebanyak 24 ekor tikus (Rattus norvegicus L) dikelompokkan menjadi 8 kelompok, terdiri atas KKN, KKV (NaCl 0,9%), KKP1 (EG 7,5%), KKP2 (EG 15%), KP1 (EG 7,5% + ML 7,5%), KP2 (EG 7,5% + ML 15%), KP3 (EG 15% + ML 7,5%), dan KP4 (EG 15% + ML 15%). Pengamatan terhadap densitas,struktur folikel serta ekspresi protein Bax,Bcl2 dan Caspase3 dilakukan terhadap sayatan ovarium yang dibuat dengan metode parafin dengan pewarnaan Hematoksilin-Eosin (HE) dan imunohistokimia. Antibodi primer yang digunakan adalah antibodi poliklonal Rabbit Anti-Bax (A00183 Boster, USA), Rabbit Anti-Bcl-2 (A00040-2 Boster, USA) dan Active Caspase-3 Rabbit Polyclonal Antibody (ab4051 Abcam, UK) dan One Step Neopoly Detection System Kit (BGNK-0025 Biogear, USA). Identifikasi terhadap tiap tipe folikel preantral menggunakan mikroskop cahaya yang terhubung dengan perangkat lunak Image Raster dan IHC profiler. Hasil penelitian menunjukkan, efek krioprotektif madu lengkeng dapat meningkatkan densitas folikel, indeks folikel intak G2 dan G3, menurunkan indeks folikel G1, menekan ekspresi protein Bax dan caspase 3 serta meningkatkan ekspresi protein Bcl2. Dengan demikian, madu lengkeng memiliki potensi untuk dikembangkan sebagai krioprotektan ekstraselular alami dalam aplikasi vitrifikasi ovarium.

---

A study was conducted to identify the cryoprotective effect of longan honey (Dimocarpus longan) on the structural integrity of the preantral follicle and the expression profile of the intrinsic pathway of apoptosis protein. A total of 24 rats (Rattus norvegicus L) were grouped into 8 groups, consisting of KKN, KKV (NaCl 0.9%), KKP1 (EG 7.5%), KKP2 (EG 15%), KP1 (EG 7.5% + LH 7.5%), KP2 (EG 7.5% + LH 15%), KP3 (EG 15% + LH 7.5%), and KP4 (EG 15% + LH 15%). Observations on the density, follicular structure and protein expression of Bax, Bcl2 and Caspase3 were carried out on ovarian sections made by paraffin method with Hematoxylin-Eosin (HE) staining and immunohistochemistry. The primary antibodies used were Rabbit Anti-Bax polyclonal antibody (A00183 Boster, USA), Rabbit Anti-Bcl-2 (A00040-2 Boster, USA) and Active Caspase-3 Rabbit Polyclonal Antibody (ab4051 Abcam, UK) and One Step Neopoly Detection System Kit (BGNK-0025 Biogear, USA). Identification of each type of preantral follicle using a light microscope connected to Image Raster software and an IHC profiler. The results showed that the cryoprotective effect of longan honey could increase follicle density, G2 and G3 intact follicle index, decrease G1 follicle index, suppress Bax protein expression and caspase 3 and increase Bcl2 protein expression. Thus, longan honey has the potential to be developed as a natural extracellular cryoprotectant in ovarian vitrification applications."

"Supervision in the Hospitality Industry: Leading Human Resources, 7th Edition focuses on the different roles of employees from beginning leaders, newly promoted supervisor, or anyone planning a career in the hospitality field. The text is enhanced from previous issues with more industry examples and the addition of key word definitions. It offers several new chapters on topics such as supervisors as leaders; goal setting; supervisory communication; suggestions for improving communications and social media communications; drivers of diversity; multiculturalism; social media recruiting; hiring suggestions; evaluating on-the-job performance and performance improvement; behaviors of leaders; team building; teaching methods for training; causes of conflict in the workplace; critical thinking example and question on discipline; supervising employees; supervisors delegating; and common mistakes in delegating"

"This book summarises the presentations of a NATO Advanced Training Course addressing the issue of food security in Central Asia. The book is divided into two sections. The first provides an overview of the existing aspects of food security in participating Central Asian countries. The emphasis here is on food safety, control and access and includes background information on the relevant food industries. Participating countries include the Kyrgyz Republic, Kazakhstan, Uzbekistan and Tajikistan. The second section explores particular aspects of food security in participating NATO countries. These provide some insight into the value, strengths and weaknesses of common food security systems. Chapters cover HACCP, ISO/IEC 17025 standards and associated pre-requisite systems, allergies and food intolerances, risk perception and communication, training, and ethics. A chapter on food defence in the USA is also included. "

"Functional foods are sometimes called nutraceuticals, a portmanteau of nutrition and pharmaceutical, and can include food that has been genetically modified. This is an emerging field in food science, in which such foods are usually accompanied by health claims for marketing purposes."

"The book will discuss the published experimental techniques, data-processing algorithms, and approaches used in FT-IR spectroscopy to assist in the characterization and identification of microorganisms, to assess the mechanisms of bacterial inactivation by food processing technologies and antimicrobial compounds, to monitor the spore and membrane properties of foodborne pathogens in changing environments, to detect stress-injured microorganisms in food-related environments, to assess dynamic changes in bacterial populations, and to study bacterial tolerance responses."