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Bugi R. Budiarto
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Background: Currently available molecular method to detect HER2I655V polymorphism such as PCR-RFLP is
hampered by costly experimental method and post-PCR treatment requirment that make this technique is not meeting
for high-throughput analysis purpose. In this study we developed a accurate, simple, low cost and rapid test to detect
polymorphism at HER2 gene using SBR Green I based-melting curve method. Methods: Two forward allele-specific
primers and one common reverse primer were used then these primers were tested to discriminate known ...
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Indonesian Institute of Science (LIPI). Research Center for Biotechnology ; Universitas Andalas. Faculty of Medicine, 2016
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