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Ditemukan 1 dokumen yang sesuai dengan query
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Andri Wardiana
Purification and carbohydrate analysis of recombinant human erythropoietin expressed in yeast system pichia pastoris
" ABSTRACT
For clinical purposes, pure protein and identification of carbohydrate structure from recombinant erythropoietin are needed. Purification was done by Immobilized Metal Affinity Chromatography (IMAC) column charged with Ni2+(His-Trap affinity chromatography) and continued with gel filtration chromatogram phy column to get purer protein. The carbohydrate group which is o ligosaccharide from the resulting pure protein then can be recognized by using N- and O-glycosidase. Pure oligosaccharide was hydrolyzed to produce various monosaccharide through incubation with 4 ... "
[Direktorat Riset dan Pengabdian Masyarakat UI;Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi;Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi], 2011
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Artikel Jurnal  Universitas Indonesia Library