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"Muara Sungai Blanakan yang berlokasi di Kabupaten Subang, Jawa Barat merupakan wilayah pesisir yang tinggi aktivitas perikanan tangkap dan tambak. Penelitian ini bertujuan untuk menganalisis kelimpahan mikroplastik di air, sedimen, dan ikan kuro (Eleutheronema tetradactylum) serta mengkaji komposisinya. Sampel air dan sedimen diambil dari 4 titik di sekitar muara bersama 12 individu ikan kuro. Sampel air dan sedimen diekstraksi menggunakan reagen Fenton. Sementara insang, saluran pencernaan dan daging yang dibedah pada tiap individu didestruksi dengan KOH 10%. Seluruh larutan sampel yang telah di destruksi disaring menggunakan vacuum pump dengan kertas saring Whatman cellulose nitrate membrane ukuran pori 0,45 μm. Identifikasi mikroplastik dilakukan dengan pengamatan melalui mikroskop untuk perhitungan kelimpahan serta komposisi mikroplastik yang dikategorikan berdasarkan bentuk, ukuran, dan warna. Sementara jenis polimer dianalisis menggunakan Raman microscopes spectrometer. Penelitian ini menunjukkan mikroplastik ditemukan pada seluruh sampel dan lokasi penelitian dengan kelimpahan yang berbeda. Rata-rata kelimpahan mikroplastik pada air didapatkan 710 ± 183,34 partikel/m3 dan pada sedimen 879,63 ± 205,14 partikel/kg. Sementara pada organ dan jaringan ikan berturut-turut dari kelimpahan terbesar diperoleh 16,64 ± 9,09 partikel/gr pada saluran pencernaan, 11,95 ± 5,33 partikel/gr pada insang, dan 3,55 ± 0,81 partikel/gr pada daging. Secara keseluruhan, komposisi mikroplastik yang ditemukan pada penelitian ini merupakan bentuk fiber, fragmen, film, dan pellet, dengan tidak ditemukannya bentuk foam. Ukuran mikroplastik yang paling banyak ditemukan pada air, sedimen, dan jaringan daging ikan merupakan <300 μm. Sementara pada insang dan saluran pencernaan, ukuran >1000 μm paling banyak ditemukan. Warna biru pada studi ini menjadi warna mikroplastik yang paling mendominasi. Polimer mikroplastik yang ditemukan merupakan polyethylene terephthalate (PET), polypropylene (PP), dan polyvinyl chloride (PVC). Uji korelasi menunjukkan tidak adanya korelasi antara kelimpahan mikroplastik di air maupun sedimen terhadap akumulasi mikroplastik pada ikan kuro.

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Blanakan River estuary in Subang Regency, West Java, Indonesia is a coastal area with a high fishing and ponds activities. This study aims to examined the abundance and composition of microplastic contamination in water, sediment, and fourfinger threadfin (Eleutheronema tetradactylum). Water and sediment samples were obtained at 4 various location around the estuary with 12 individual fourfinger threadfin fish. Water and sediment samples were extracted using Fenton's reagent. The dissected gills, digestive tracts and muscles were destroyed with 10% KOH. All digested sample solution are filtered using a vacuum pump and Whatman cellulose nitrate membrane filter paper with a 0.45 μm pore size. Identification of microplastics was carried out by microscope observation to calculate the abundance and composition of microplastics which were categorized based on shape, size and color. While the type of polymer was analyzed using a Raman microscopes spectrometer. This study demonstated that microplastics were found at all samples and research locations with different abundances. The average abundance of microplastics in water was 710 ± 183.34 particles/m3 and in the sediment sample was 879.63 ± 205.14 particles/kg. While in fish, the highest abundance was found in the digestive tract at 16.64 ± 9.09 particles/gr, followed gills at 11.95 ± 5.33 particles/gr, and muscle at 3.55 ± 0.81 particles/gr. Overall, the composition of microplastics form consisted of fiber, fragments, films, and pellets; foam was absent in this study. The most frequent size of microplastic found in water, sediment, and fish tissue is <300 μm. While sizes greater than 1000 μm are most frequently observed in the gills and digestive tract. In this study, blue is the predominant microplastic color. Microplastic polymers detected were polyethylene terephthalate (PET), polypropylene (PP), and polyvinyl chloride (PVC). The correlation test showed that there was no correlation between the abundance of microplastics in water and sediment with the accumulation of microplastics in fourfinger threadfin fish."

"Plastik merupakan bahan stabilitas tinggi hasil polimerisasi monomer dengan tingkat penggunaan yang tinggi. Sampah plastik berbahaya bagi lingkungan karena partikel penyusunnya memiliki ketahanan dan kestabilan tinggi sehingga proses degradasinya berlangsung lama. Di lingkungan perairan, plastik akan mengalami degradasi atau penguraian menjadi partikel kecil yang disebut mikroplastik (<5 mm). Partikel mikroplastik berpotensi termakan oleh berbagai biota perairan sehingga membahayakan siklus rantai makanan melalui proses biomagnifikasi. Penelitian ini bertujuan untuk menganalisis karakteristik mikroplastik (bentuk, ukuran, dan jenis polimer) pada air, sedimen dan ikan belanak Mugil cephalus (Linnaeus, 1758) pada organ dan jaringan (daging, insang, saluran pencernaan) di Muara Sungai Blanakan, Subang, Jawa Barat. Metode penelitian ini diawali dengan pengambilan sampel dengan  air  diambil sebanyak 50 L lalu disaring menggunakan plankton net hingga tertampung volume air 1000 mL, sampel sedimen diambil menggunakan Vanveen grab hingga tertampung pada jar 500 mL dan sampel ikan belanak diambil 10 ekor menggunakan bubu. Ekstraksi sampel sedimen dilakukan dengan pemberian larutan NaCl jenuh dengan perbandingan 1 (sedimen): 2 (NaCl jenuh), kemudian diberi larutan H2O2 30% + FeSO4 0,05 M  dengan perbandingan 1:1 untuk sampel air dan sedimen. Ekstraksi sampel ikan dilakukan dengan mengambil jaringan dan organ yang digunakan, ditimbang dan dan diberi larutan KOH 10% sebanyak 50 mL. Sampel air, sedimen dan ikan disaring menggunakan kertas saring Whatman dan diidentifikasi mikroplastik menggunakan mikroskop olympus CX22LED. Analisis polimer mikroplastik dilakukan dengan metode Raman Spectroscopy. Uji statistik seperti uji kruskal-walis, one way anova, dan uji regresi spearman dan pearson digunakan untuk menganalisis data yang diperoleh. Hasil penelitian menunjukkan rata-rata total kelimpahan mikroplastik pada air 710 ± 183,34  partikel meter-3, sedimen 879,63 ± 205,13 partikel Kg-1 dan ikan belanak 210,8 ± 108,80 partikel individu-1. Nilai kelimpahan mikroplastik ikan belanak jika diurutkan dari yang tertinggi hingga terkecil adalah daging, saluran pencernaan, dan insang. Secara keseluruhan, bentuk dan ukuran mikroplastik yang paling banyak ditemukan adalah fiber dan <300 µm. Polimer mikroplastik yang dominan adalah PET, PP, dan PVC. Berdasarkan hasil uji beda nyata kelimpahan mikroplastik antar organ dan jaringan ikan belanak menunjukan tidak memiliki perbedaannya yang signifikan. Hasil uji korelasi Spearman menunjukan adanya korelasi antara kelimpahan mikroplastik di air dan sedimen Muara Sungai Blanakan terhadap kelimpahan mikroplastik di ikan belanak.

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Plastik is a high-stability material resulting from the polymerization of monomers, with a high level of usage. In aquatic environments, plastic undergoes photo-oxidative degradation by UV radiation from the sun and chemical processes, leading to the breakdown of plastic waste into small particles known as microplastics (<5 mm). Microplastic particles have the potential to be ingested by various aquatic organisms, posing a risk to the food chain through biomagnification. This study aims to analyze the characteristics of microplastics in water, sediment, and the flathead grey mullet (Mugil cephalus) in different organs and tissues (muscle, gills, digestive tract) in the Blanakan River Estuary, Subang, West Java, based on their shape, size, and polymer types. Water samples were collected in a volume of 50 L, filtered using a plankton net to obtain a final volume of 1000 mL. Sediment samples were collected using a Vanveen grab and stored in 500 mL jars, while flathead grey mullet samples were collected using bubu (10 individuals). Sediment sample extraction was performed using a saturated NaCl solution with a ratio of 1 (sediment) to 2 (saturated NaCl solution), followed by the addition of a 30% H2O2 + 0.05 M FeSO4 solution in a 1:1 ratio for water and sediment samples. Fish sample extraction involved weighing and placing the tissues and organs in a glass beaker, followed by the addition of a 10% KOH solution in a volume of 50 mL. The water, sediment, and fish samples were then filtered using Whatman filter paper with the assistance of a vacuum pump, and placed in Petri dishes for microplastic identification using an Olympus CX22LED microscope. Polymer analysis of microplastics was performed using Raman Spectroscopy. Kruskal-Wallis and one-way ANOVA tests were used to determine significant differences in the abundance and composition of microplastics (size and shape) in water, sediment, flathead grey mullet, and their respective organs. Spearman and Pearson correlation tests were used to investigate the influence of water and sediment, as well as morphometric values, on microplastic accumulation in flathead grey mullet. The research findings showed an average total abundance of microplastics in water to be 710 ± 183,34 particles meter-3, in sediment to be 879,63 ± 205,13 particles kilogram-1, and in flathead grey mullet to be 210,8 ± 108,80 particles individual-1. When ranked in descending order, the abundance of microplastics in the three parts of the flathead grey mullet were as follows: flesh, digestive tract, and gills. Overall, the most commonly found forms and sizes of microplastics were microplastic fibers and those below 300 µm. The polymer analysis revealed that the microplastics found in the Blanakan River Estuary were dominated by PET (40%),  PP (40%), and PVC (20%). Based on the significant difference test for microplastic abundance among different parts of the flathead grey mullet, no significant differences were found. The Spearman correlation test indicated a correlation between the abundance of microplastics in water and sediment in the Blanakan River Estuary and the abundance of microplastics in flathead grey mullet."

"Plastik merupakan salah satu barang yang memiliki tingkat penggunaan yang tinggi hingga mencapai skala produksi sebesar 300 juta ton pertahun. Limbah plastik di perairan membutuhkan waktu hingga ratusan tahun untuk terdegradasi karena karakteristiknya yang kuat, tahan lama, dan tahan terhadap suhu tinggi. Proses degradasi limbah plastik di perairan disebabkan oleh sinar UV yang menyebabkan terjadinya perubahan bentuk dan ukuran menjadi lebih kecil (mikroplastik) atau tercampur di dalam air (< 5mm). Ukuran yang kecil menyebabkan mikroplastik dapat dengan mudah termakan oleh biota perairan, seperti ikan atau melalui mangsanya yang lebih kecil. Penelitian ini bertujuan untuk menganalisis komposisi dan kelimpahan mikroplastik berdasarkan bentuk, ukuran, dan polimer pada air, sedimen, serta organ dan jaringan ikan kiper Scatophagus argus (Linnaeus, 1766) di muara Sungai Blanakan, Subang, Jawa Barat. Metode penelitian dilakukan dengan pengambilan sampel air sebanyak 50 L lalu disaring menggunakan plankton net hingga mendapatkan 1 L sampel air, pengambilan sedimen dilakukan menggunakan Van Veen grab lalu dimasukkan ke dalam jar HDPE 500 ml, dan pengambilan sampel ikan sebanyak 10 individu dilakukan menggunakan alat tangkap bubu. Sampel air ditambahkan larutan H2O2 30% dan FeSO4 0,05 M masing-masing sebanyak 25 ml. Sampel sedimen dicampurkan larutan NaCl dengan perbandingan sebesar 1 : 2 dan didiamkan. H2O2 30% dan FeSO4 0,05 M ditambahkan hingga sampel terendam. Sampel ikan dibedah untuk diambil bagian insang, saluran pencernaan, dan daging. Setiap bagian dihancurkan menggunakan larutan KOH 10% dengan perbandingan 1 : 10 dan didiamkan. Sampel air, sedimen, dan ikan dipipet sebanyak 40 ml dan disaring menggunakan kertas saring cellulose nitrate dengan bantuan vacuum pump lalu kertas saring ditempatkan di cawan petri. Sampel air, sedimen, dan ikan kiper diamati pada kertas saring menggunakan mikroskop Olympus CX22 dan analisis polimer mikroplastik dilakukan dengan metode Raman Spectroscopy. Analisis statistik dilakukan menggunakan uji one way ANOVA, Kruskal wallis, dan Pearson. Hasil penelitian menunjukkan rerata kelimpahan mikroplastik pada sampel air sebesar 710 partikel/m3, sedimen sebesar 879,63 partikel/kg, dan ikan sebesar 74,77 partikel/individu. Kelimpahan mikroplastik dari yang tertinggi hingga terkecil pada ikan kiper terdapat di daging, saluran pencernaan, dan insang. Komposisi mikroplastik paling dominan berupa bentuk fiber dan ukuran < 300 µm. Polimer yang ditemukan bertipe PET, PP, dan PVC. Uji Kruskal wallis menunjukkan terdapat perbedaan secara nyata kelimpahan mikroplastik antar organ dan jaringan ikan. Uji Pearson menunjukkan adanya korelasi kelimpahan mikroplastik pada air dan sedimen terhadap kelimpahan mikroplastik di ikan.

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Plastic is one of the commodities with a high level of usage, reaching a production scale of 300 million tons per year. Plastic waste in water takes hundreds of years to degrade due to its strong, durable, and heat-resistant characteristics. The degradation process of plastic waste in water is caused by UV radiation, which leads to changes in shape and size, resulting in smaller particles (microplastics) or mixing with water (< 5mm). The small size of microplastics makes them easily ingestible by aquatic organisms, such as fish, or through their prey, which are smaller in size. This research aims to analyze the composition and abundance of microplastics based on their form, size, and polymer in water, sediments, as well as the organs and tissues of the Scatophagus argus(Linnaeus, 1766) fish in the estuary of the Blanakan River, Subang, West Java. The research method involved collecting 50 liters of water samples, which were then filtered using a plankton net to obtain a 1-liter water sample. Sediment samples were collected using a Van Veen grab and placed in a 500 ml HDPE jar, while fish samples were collected using a fish trap, with a total of 10 individuals. The water sample was treated with 25 ml of 30% H2O2 and 0.05 M FeSO4 solutions. The sample was mixed with a NaCl solution in a ratio of 1:2 and left to settle. H2O2 (30%) and FeSO4 (0.05 M) were added until the sample was fully submerged. The samples were dissected to obtain gill, digestive tract, and muscle tissue. Each part was crushed using a 10% KOH solution in a 1:10 ratio and left to settle. Water, sediment, and fish samples were pipetted (40 ml) and filtered using cellulose nitrate filter paper with the assistance of a vacuum pump, and the filter papers were placed in Petri dishes. The water, sediment, and fish samples were observed on the filter paper using an Olympus CX22 microscope, and the analysis of microplastic polymers was conducted using Raman Spectroscopy. Statistical analysis was performed using one-way ANOVA, Kruskal-Wallis, and Pearson tests. The results of the study showed that the average abundance of microplastics in water samples was 710 particles/m3, in sediments it was 879.63 particles/kg, and in fish it was 74.77 particles/individual. The highest abundance of microplastics in the kiper fish was found in the muscle tissue, followed by the digestive tract and gills. The dominant composition of microplastics was in the form of fibers and with a size of < 300 µm. The polymers found were PET, PP, and PVC. The Kruskal-Wallis test indicated a significant difference in microplastic abundance among the organs and tissues of fish. The Pearson test showed a correlation between the abundance of microplastics in water and sediments with the abundance of microplastics in fish."

"Penelitian ini dilatarbelakangi oleh meningkatnya polusi plastik yang menyebabkan pencemaran mikroplastik di perairan air tawar. Situ Rawa Besar, Depok merupakan salah satu situ yang tercemar oleh mikroplastik akibat aktivitas manusia di sekitarnya. Mikroplastik dapat berdampak negatif bagi organisme akuatik seperti ikan sapu-sapu Pterygoplichthys pardalis yang merupakan spesies invasif dan bioindikator pencemaran mikroplastik di situ. Penelitian ini bertujuan untuk menganalisis kelimpahan, bentuk dan warna mikroplastik pada air, sedimen, insang dan saluran pencernaan ikan sapu-sapu di Situ Rawa Besar serta menguji perbedaan kelimpahan mikroplastik pada tahun 2022 dengan 2023. Penelitian ini menggunakan metode deskriptif kuantitatif untuk menganalisis kelimpahan, bentuk dan warna mikroplastik pada air, sedimen, insang, dan saluran pencernaan sapu-sapu di Situ Rawa Besar. Pengambilan sampel dilakukan di tiga stasiun. Parameter fisika-kimia air seperti suhu, pH, oksigen terlarut, dan kecerahan air diukur secara in situ. Sampel air 20 L disaring menggunakan plankton net dan sampel sedimen diambil menggunakan Ekman grab dan dikeringkan dengan oven, lalu ditambahkan larutan NaCl jenuh. Sampel ikan sapu-sapu diambil sebanyak 15 ekor dengan menggunakan jaring ikan. Sampel insang dan saluran pencernaan ikan dipisahkan dengan menggunakan dissecting set dan dilarutkan dengan HNO3 kemudian ditambahkan larutan NaCl jenuh. Perhitungan partikel mikroplastik dilakukan di bawah mikroskop dan sampel diletakkan pada Sedgwick Rafter Chamber. Mikroplastik dianalisis kelimpahannya dan diklasifikasikan menurut bentuknya (pellet, fiber, film, dan fragmen) serta dihitung persentase komposisi mikroplastik dalam sampel. Hasil penelitian pada tahun 2022 menunjukkan bahwa kelimpahan mikroplastik berkisar antara 12,67 – 20,33 partikel L-1 pada air, 14.400,00-38.400,00 partikel kg-1 pada sedimen, 250,67-386,67 partikel ind-1 pada insang, dan 313,33-369,33 partikel ind-1 pada saluran pencernaan. Kelimpahan mikroplastik tersebut lebih rendah dari kelimpahannya pada tahun 2023, yaitu berkisar antara 14,67-32,00 partikel L-1 pada air, 16.533,33-41.600,00 partikel kg-1 pada sedimen, 333,33-446,67 partikel ind-1 pada insang, dan 429,33-502,67 partikel ind-1 pada saluran pencernaan. Bentuk mikroplastik yang paling dominan adalah fiber pada air dan ikan sapu-sapu, fragmen pada sedimen. Warna mikroplastik yang paling dominan adalah biru pada air dan sedimen, hitam pada insang dan saluran pencernaan ikan sapu-sapu. Kelimpahan mikroplastik pada tahun 2023 lebih tinggi dibandingkan tahun 2022, dengan peningkatan sebesar 15,24% pada insang, 24,25% pada saluran pencernaan, 27,91% pada air, dan 7,12% pada sedimen. Penelitian ini menunjukkan peningkatan jumlah mikroplastik di Situ Rawa Besar seiring berjalannya waktu yang didukung oleh beberapa faktor seperti peningkatan populasi, peningkatan sampah plastik, aktivitas antropogenik, dan curah hujan.

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This research was motivated by the increasing plastic pollution that causes microplastic contamination in freshwater bodies. Situ Rawa Besar, Depok is one of the lakes that is polluted by microplastics due to human activities around it. Microplastics can have negative impacts on aquatic organisms such as Amazon Sailfin Catfish Pterygoplichthys pardalis which is an invasive species and a bioindicator of microplastic pollution in the lake. This research aims to analyze the abundance, shape and color of microplastics in water, sediment, gills and digestive tract of sailfin catfish in Situ Rawa Besar and to test the difference in microplastic abundance between 2022 and 2023. This research uses descriptive quantitative method to analyze the abundance, shape and color of microplastics in water, sediment, gills, and digestive tract of sailfin catfish in Situ Rawa Besar. Sampling was done at three stations. Physico-chemical parameters of water such as temperature, pH, dissolved oxygen, and water clarity were measured in situ. Water samples of 20 L were filtered using plankton net and sediment samples were taken using Ekman grab and dried with oven, then added with saturated NaCl solution. Sailfin catfish samples were taken as many as 15 individuals using fish net. Gill and digestive tract samples were separated using dissecting set and dissolved with HNO3 then added with saturated NaCl solution. Microplastic particle counting was done under microscope and samples were placed on Sedgwick Rafter Chamber. Microplastics were analyzed for their abundance and classified according to their shape (pellet, fiber, film, and fragment) and the percentage composition of microplastics in the sample was calculated. The results of the research in 2022 showed that the abundance of microplastics ranged from 12.67-20.33 particles L-1 in water, 14,400.00-38,400.00 particles kg-1 in sediment, 250.67-386.67 particles ind-1 in gills, and 313.33-369.33 particles ind-1 in digestive tract. The abundance of microplastics was lower than its abundance in 2023, which ranged from 14.67-32.00 particles L-1 in water, 16,533.33-41,600.00 particles kg-1 in sediment, 333.33-446.67 particles ind-1 in gills, and 429.33-502.67 particles ind-1 in digestive tract. The most dominant shape of microplastics was fiber in water and sailfin catfish, fragment in sediment and digestive tract of sailfin catfish. The most dominant color of microplastics was blue in water and sediment, black in gills and digestive tract of sailfin catfish. The abundance of microplastics increased from 2022 to 2023, with an increase of 15.24% in gills, 24.25% in digestive tract, 27.91% in water, and 7.12% in sediment. This research shows an increase in the number of microplastics in Situ Rawa Besar over time supported by several factors such as population growth, increased plastic waste, anthropogenic activities, and rainfall."

"Mikroplastik didefinisikan sebagai partikel plastik dengan ukuran <5 mm. Mikroplastik dapat masuk ke dalam tubuh organisme air khususnya ikan melalui oral dan insang. Penelitian ini bertujuan untuk mengetahui bentuk dan kelimpahan mikroplastik pada air, insang dan saluran pencernaan ikan mujair Oreochromis mossambicus (Peters, 1852) serta menguji perbedaan kelimpahan mikroplastik pada ikan mujair di Danau Kenanga dan Danau Agathis Universitas Indonesia. Penelitian dilakukan di Departemen Biologi FMIPA UI, Depok. Sampel ikan mujair diperoleh sebanyak total 20 ekor dari kedua danau dengan panjang tubuh relatif antara 16--19 cm. Insang dan saluran pencernaan dari 20 sampel ikan mujair diisolasi, kemudian diekstraksi dan dihancurkan menggunakan larutan asam nitrat (HNO3) 65%. Sampel yang telah diekstraksi kemudian diberi larutan NaCl jenuh agar mikroplastik mengapung ke permukaan. Sampel diteteskan sebanyak 1 ml pada Sedgwick Rafter Chamber kemudian diamati di bawah mikroskop dan dihitung jumlah mikroplastik berdasarkan bentuk partikelnya. Total kelimpahan rata-rata mikroplastik pada air Danau Kenanga sebesar 1.766,6 ± 40,11 partikel/L, sementara total kelimpahan rata-rata mikroplastik air pada Danau Agathis sebesar 1.885,53 ± 106,27 partikel/L. Total kelimpahan rata-rata mikroplastik ikan mujair pada Danau Kenanga di insang sebanyak 6.232 ± 1.898,66 partikel/ind dan di saluran pencernaan sebanyak 9.108 ± 4.027,14 partikel/ind, sementara total kelimpahan rata-rata mikroplastik ikan mujair pada Danau Agathis di insang sebanyak 6.716 ± 2.467,67 partikel/ind dan di saluran pencernaan sebanyak 4.038 ± 2.180,75 partikel/ind. Persentase komposisi bentuk mikroplastik yang ditemukan dominan pada Danau Kenanga terdapat bentuk fragmen sebesar 40% di air; fiber 80% di insang dan fiber 75% di saluran pencernaan, Sementara komposisi bentuk mikroplastik yang ditemukan dominan pada Danau Agathis terdapat bentuk fiber sebesar 43% di air; 75% di insang dan 67% di saluran pencernaan. Hasil analisis statistik Uji Mann-Whittney menunjukkan adanya perbedaan secara signifikan kelimpahan mikroplastik pada ikan mujair di Danau Kenanga dan Danau Agathis, Universitas Indonesia.

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Microplastics are define as plastic particles with a size of <5 mm. Microplastics can enter the body of aquatic organisms, especially fish through the mouth and gills. This study aims to determine the shape and abundance of microplastics in water, gill and digestive tract tilapia fish Oreochromis mossambicus (Peters, 1852) at Kenanga Pond and Agathis Pond, University of Indonesia and to analyze and examine differences in the abundance of microplastics in tilapia fish at Kenanga Pond and Agathis Pond, University of Indonesia. The study was conducted at Department of Biology FMIPA UI, Depok. Total 20 tilapia fish from the two lakes with relative length between 16--19 cm. Gills and digestive tract samples were obtained of tilapia fish were isolated, extracted and then crushed using a saturated solution of nitric acid (HNO3) 65%. The extracted sample is then given NaCl solution so that the microplastic floats to the surface. As much as 1 ml NaCl were dropped in the Sedgwick Rafter Chamber then observed under a microscope. The number of microplastic was calculated based on the type. The average total abundance of microplastics in the water of Kenanga Pond was 1.766,6 ± 40,11 particles/L, meanwhile the average total abundance of microplastics in the water of Agathis Pond was 1.885,53 ± 106,27 particles/L. The average total microplastics in tilapia gill at Kenanga Pond was 6.232 ± 1.898,66 particles/ind and in the digestive tract was 9.108 ± 4,027.14 particles/ind, meanwhile the average total abundance microplastics in tilapia gill at Agathis Pond was 6.716 ± 2.467,67 particles/ind and in the digestive tract was 4.038 ± 2.180,75 particles/ind. The percentage composition of the microplastic form that was found dominant at Kenanga Pond were as much as 40% fragments in the water; 80% fiber in the gills and 75% fiber in the digestive tract. Meanwhile, the percentage composition of the microplastic form that was found dominant at Agathis Pond were as much as 43% fiber in the water; 75% in the gills and 67% in the digestive tract. The results of the Mann-Whittney test statistical analysis showed that there was significant difference between abundance of microplastics in tilapia fish at of Kenanga Pond and Agathis Pond, University of Indonesia."

"Mikroplastik merupakan partikel plastik yang berukuran kurang dari 5 mm dan menjadi salah satu polutan di badan air. Mikroplastik termasuk emerging contaminant dan keberadaannya telah ditemukan di berbagai sungai. Sungai digunakan oleh manusia sebagai sumber air baku untuk air minum sehingga kualitasnya harus terjaga untuk mencegah dampak buruknya bagi manusia. Mikroplastik yang terakumulasi pada tubuh manusia dapat menyebabkan peradangan pada organ, cedera internal dan/atau eksternal, transformasi kandungan kimia plastik ke dalam tubuh, dan penurunan kesuburan. Penelitian perlu dilakukan di salah satu sungai dengan dampak terbesar di Jakarta, yaitu Sungai Ciliwung yang digunakan sebagai sumber air baku bagi masyarakat Jakarta. Penelitian ini bertujuan untuk menganalisis jumlah partikel mikroplastik, mengidentifikasi karakteristik partikel mikroplastik berdasarkan jenis, wana, dan material, serta menganalisis pengaruh kondisi cuaca terhadap kelimpahan partikel mikroplastik. Objek penelitian ini adalah air dan sedimen di Sungai Ciliwung dengan 7 titik lokasi pengambilan sampel dan 2 waktu pengambilan sampel. Pengambilan sampel air menggunakan plankton net untuk menyaring air sebanyak 10 liter. Preparasi sampel air dilakukan dengan cara memisahkan mikroplastik berdasarkan densitas, menghilangkan kandungan organik, menyaring dengan kertas saring, lalu menganalisis mikroplastik dengan mikroskop. Pengambilan sampel sedimen menggunakan Ekman grab sampler sebanyak 400 ml. Pengujian sampel sedimen hampir sama dengan sampel air dengan adanya penambahan pengeringan sampel menggunakan oven pada awal preparasi sampel. Analisis kelimpahan mikroplastik menggunakan metode adaptasi NOAA untuk air dan sedimen sungai. Identifikasi karakteristik material mikroplastik menggunakan uji FTIR untuk menghasilkan gugus ikatan kimia material. Analisis hubungan kelimpahan mikroplastik dengan cuaca dilakukan dengan paired t-test. Penelitian menghasilkan rata-rata kelimpahan pada air sebanyak 1.111 partikel/L dan pada sedimen sebanyak 1.583 partikel/100 gram. Jenis mikroplastik di Sungai Ciliwung ada fragmen, fiber, film, microbeads, dan foam dengan jenis paling mendominasi yaitu fragmen. Warna mikroplastik yang teridentifikasi di Sungai Ciliwung ada hitam, merah, biru, hijau, kuning, dan transparan, serta warna hitam merupakan warna dominan pada sampel. Material yang ditemukan pada sampel adalah Tencel, cellopha, cupra, PTFE, FEP, PVFM, dan silicon. Tencel adalah material yang ditemukan pada semua sampel. Cuaca tidak mempengaruhi kelimpahan mikroplastik pada kondisi curah hujan tinggi dan rendah.

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Microplastic is plastic particle that is less than 5 mm in size and is one of the pollutants in water bodies. Microplastics are emerging contaminants and their presence has been found in various rivers. Rivers are used by humans as a source of raw water for drinking water so that its quality must be maintained to prevent adverse effects on humans. Microplastics that accumulate in human body can cause inflammation of organs, internal and/or external injury, transformation of plastic chemical substances in human body, and decreased fertility. Research needs to be conducted in one of the rivers with the greatest impact in Jakarta, the Ciliwung River, which is used as a source of raw water for the people in Jakarta. This study aims to analyze the number of microplastic particles, identify the characteristics of microplastic particles based on type, color, and material, and analyze the effect of weather conditions on the abundance of microplastic particles. The object of this research is water and sediment in the Ciliwung River with 7 sampling locations and 2 sampling times. Water sampling used a plankton net to filter 10 liters of water. Preparation of water samples is done by separating microplastics based on density, removing organic content, filtering with filter paper, then analyzing microplastics with a microscope. Sediment sampling uses an Ekman grab sampler in the amount of 400 ml. Sediment sample testing is almost the same as water samples with the addition of drying samples using an oven at the beginning of sample preparation. Analysis of microplastic abundance uses the NOAA adaptation method for water and river sediments. Identification of microplastic material characteristics uses the FTIR test to produce material chemical bond. Analysis of the relationship between microplastic abundance and weather is done with paired t-test. The study resulted in an average abundance in water of 1,111 particles/L and in sediment of 1,583 particles/100 grams. The types of microplastics in the Ciliwung River are fragments, fibers, films, microbeads, and foam with the most dominating type is fragments. The color of microplastics identified in the Ciliwung River is black, red, blue, green, yellow, and transparent, and black is the dominant color in the sample. The materials found in the samples are Tencel, cellopha, cupra, PTFE, FEP, PVFM, and silicon. Tencel was the material found in all samples. Weather did not affect the abundance of microplastics in high and low rainfall conditions. "

"Telah dilakukan penelitian yang bertujuan untuk membandingkan kelimpahan, bentuk, dan warna mikroplastik yang terkandung pada air, sedimen, dan keong mas Pomacea canaliculata di Situ Kenanga dan Situ Mahoni, Kampus Universitas Indonesia, Depok. Pengambilan sampel air, sedimen dan keong mas Pomacea canaliculata dilakukan di 3 titik lokasi yaitu inlet, midlet, dan outlet. Sampel air (20 l) disaring dengan plankton net 350 mesh, sampel sedimen dimasukkan ke jar 250 ml menggunakan Ekman grab lalu dioven dan dihaluskan. Sampel keong mas dikoleksi sebanyak 20 sampel setiap Situ, kemudian dianalisis dengan melarutkannya pada HNO3 65%, lalu sampel dijenuhkan dengan NaCl agar mikroplastik dapat mengapung ke permukaan. Sampel dihomogenisasi (20 ml) dan selanjutnya 1 ml diletakkan di Sedgewick Rafter Chamber untuk diamati di bawah mikroskop dan dihitung kelimpahan mikroplastik, bentuk dan warnanya. Hasil dari penelitian, kelimpahan mikroplastik di Situ Kenanga sejumlah 434,33± 23,51 partikel L-1 pada air, 45.837,04 ± 36.305,97 partikel Kg-1 pada sedimen dan 1.320,33 ± 533,91 partikel Ind-1 dan 116,19 ± 37,1 partikel pergram Ind-1 pada keong mas. Kelimpahan mikroplasik di Situ Mahoni pada air sejumlah 437,67 ± 30,21 partikel L-1, pada sedimen sejumlah 36.237,04 ± 16.702,59 partikel Kg-1, dan keong mas sejumlah 1.301,67 ± 200,72 partikel Ind-1 dan 148,38 ± 40,00 partikel pergram Ind-1. Hasil uji t dari sampel air, sedimen dan keong mas perindividu tidak terdapat perbedaan, sedangkan keong mas pergram ind-1 terdapat perbedaan. Bentuk mikroplastik yang ditemukan diantaranya adalah Fiber, fragmen, film, dan granula. Warna mikroplastik didominasi oleh hitam dan tidak berwarna.

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A study was conducted to compare the abundance, shape, and color of microplastics contained in water, sediment, and gold snail Pomacea canaliculata in Situ Kenanga and Situ Mahoni, University of Indonesia Campus, Depok. Sampling of water, sediment and gold snail Pomacea canaliculata was carried out at 3 locations, namely inlet, midlet, and outlet. The water sample (20 l) was filtered with a 350 mesh plankton net, the sediment sample was put into a 250 ml jar using an Ekman grab and then baked and mashed. The gold snail samples were collected as many as 20 samples each Situ, then analyzed by dissolving them in 65% HNO3, then the samples were saturated with NaCl so that the microplastics could float to the surface. The saturated sample was homogenized (20 ml) and then 1 ml was placed in the Sedgewick Rafter Chamber to be observed under a microscope and the microplastic abundance, shape and color were calculated. The results of the study, the abundance of microplastics in Situ Kenanga were 434.33 ± 23.51 particles L-1 in water, 45,837.04 ± 36,305.97 particles Kg-1 in sediments and 1,320.33 ± 533.91 particles Ind-1 and 116.19 ± 37.1 particles per gram Ind-1 in gold snails. The abundance of microplastics in Situ Mahoni in water was 437.67 ± 30.21 particles L-1, in sediments was 36.237.04 ± 16.702.59 particles Kg-1, and golden snails were 1,301.67 ± 200.72 Ind-1 particles and 148.38 ± 40.00 particles per gram Ind-1. The results of the t-test of water, sediment and individual gold snails were not different, while the gold snails per gram were different. The forms of microplastics found included fiber, fragments, films, and granules. The color of microplastics is dominated by black and colorless."

"Analisis Kelimpahan Mikroplastik pada Insang dan Saluran Pencernaan Ikan Sapu-sapu Pterygoplichtys pardalis (Castelnau, 1855), Air, dan Sedimen di Ciliwung Daerah Tanjung Barat dan Daerah MT Haryono, Jakarta Selatan

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Abundance Analysis of Microplastic in Gills dan Digestive Tract of Sailfin Catfish Pterygoplichthys pardalis (Castelnau, 1855), Water, and Sediment in Tanjung Barat Area and MT Haryono Area, Ciliwung, South Jakarta"

"Pencemaran mikroplastik menjadi ancaman bagi kehidupan biota. Mikroplastik yang terakumulasi dalam saluran pencernaan biota dapat menghambat pertumbuhan, mempengaruhi reproduksi, dan kerusakan organ internal. Penelitian ini bertujuan untuk mengetahui kelimpahan dan  bentuk mikroplastik pada air, sedimen, insang, dan saluran pencernaan Ikan Nila Oreochromis niloticus di Situ Rawa Besar, Depok. Sampel air dan sedimen di ambil pada 3 stasiun pengambilan sampel dari situ. Sampel Ikan Nila diambil 5 ekor masing-masing pada 3 stasiun. Sampel insang dan saluran pencernaan dihancurkan menggunakan larutan asam nitrat (HNO3) 65%. Sampel air diambil 20 L dan disaring menggunakan plankton net. Sampel sedimen dikeringkan dalam oven sebanyak 25 gram. Larutan NaCl jenuh digunakan untuk memisahkan mikroplastik dengan pengotor. Sebanyak 1 mL sampel diteteskan pada Sedgewick Rafter Chamber kemudian diamati dan dihitung berdasarkan bentuk mikroplastik di bawah mikroskop. Hasil penelitian pada Juli 2022 menunjukkan bahwa kelimpahan mikroplastik berkisar antara 15,00-23,33 partikel L-1 pada air, 20.330,00-45.330,00 partikel kg-1 pada sedimen, 182,67-593,33 partikel ind-1 pada insang, dan 596,00-701,33 partikel ind-1 pada saluran pencernaan. Kelimpahan mikroplastik tersebut lebih rendah dari kelimpahannya pada September 2023, yaitu berkisar antara 24,33-34,67 partikel L-1 pada air, 31.000,00-65.670,00 partikel kg-1 pada sedimen, 317,33-744,00 partikel ind-1 pada insang, dan 844,00-1.120,00 partikel ind-1 pada saluran pencernaan. Bentuk mikroplastik yang paling dominan adalah fragmen pada air, Ikan Nila, dan sedimen. Penelitian ini menunjukkan peningkatan jumlah mikroplastik di Situ Rawa Besar seiring berjalannya waktu yang didukung oleh beberapa faktor seperti peningkatan populasi, peningkatan sampah plastik, aktivitas antropogenik, dan curah hujan.

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Microplastic pollution poses a threat to the life of aquatic organisms. Accumulated microplastics in the digestive tracts of these organisms can hinder growth, affect reproduction, and cause internal organ damage. This research aims to determine the abundance and forms of microplastics in water, sediment, gills, and digestive tracts of Nile Tilapia (Oreochromis niloticus) in Situ Rawa Besar, Depok. Water and sediment samples were collected from three sampling stations in the lake. Five Nile Tilapia samples were taken from each station. Gill and digestive tract samples were digested using 65% nitric acid (HNO3) solution. Twenty liters of water were collected and filtered using a plankton net. Sediment samples were dried in an oven, with 25 grams used for analysis. A saturated NaCl solution was employed to separate microplastics from impurities. One milliliter of the sample was dropped onto a Sedgewick Rafter Chamber, then observed and counted based on microplastic forms under a microscope. The research results in July 2022 indicated that microplastic abundance ranged from 15.00 to 23.33 particles L-1 in water, 20,330.00 to 45,330.00 particles kg-1 in sediment, 182.67 to 593.33 particles ind-1 in gills, and 596.00 to 701.33 particles ind-1 in the digestive tract. These abundances were lower than those in September 2023, ranging from 24.33 to 34.67 particles L-1 in water, 31,000.00 to 65,670.00 particles kg-1 in sediment, 317.33 to 744.00 particles ind-1 in gills, and 844.00 to 1,120.00 particles ind-1 in the digestive tract. The most dominant form of microplastics was fragments in water, Nile Tilapia, and sediment. This research indicates an increase in the quantity of microplastics in Situ Rawa Besar over time, supported by factors such as population growth, increased plastic waste, anthropogenic activities, and rainfall."

"Keberadaan mikroplastik telah mencemari dan mengganggu perairan di wilayah Indonesia. Penelitian ini dilakukan untuk mengetahui perbandingan kelimpahan mikroplastik pada lamun Cymodocea rotundata, sedimen, dan air Pulau Rambut, Teluk Jakarta tahun 2022 dan 2023. Pengambilan sampel dilakukan di tiga stasiun Pulau Rambut (Timur, Selatan, dan Barat).  Sampel diambil sebanyak delapan individu lamun per stasiun, sedimen, dan air dengan metode random sampling.  Sampel lamun dipotong sepanjang 2 cm lalu dikerik, sedimen dikeringkan lalu diberikan larutan jenuh NaCl, dan sempel air diberikan NaCl kemudian seluruh sampel diamati dibawah mikroskop. Rata-rata kelimpahan mikroplastik lamun C. rotundata pada tahun 2022 sebesar 42 partikel/cm dan pada tahun 2023 sebesar 44,46 partikel/cm. Rata-rata kelimpahan mikroplastik pada sedimen tahun 2022 sebesar 73,53 partikel/g dan pada tahun 2023 sebesar  79,56 partikel/g. Kelimpahan mikroplastik pada sampel air tahun 2022 sebesar 51,33 partikel/L dan pada tahun 2023 sebesar 53,78 partikel/L. Uji Korelasi Spearman menjelaskan bahwa kelimpahan mikroplastik sampel lamun dengan sedimen, air dengan sedimen, dan lamun dengan air memiliki korelasi positif yang kuat. Hasil Uji-T menyatakan terdapat perbedaan tidak signifikan kelimpahan mikroplastik pada lamun C. rotundata (sig. (2-tailed) 0,182>0,05) dan terdapat perbedaan signifikan kelimpahan mikroplastik sedimen tahun 2022 dan 2023 (sig. (2-tailed) 0,007<0,05). Hasil uji ATR-FTIR didapatkan kandungan polimer CA, ABS, HDPE, PMMA, PVC, dan PET pada sampel daun lamun C. rotundata.

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The existence of microplastics has polluted and disturbed the waters in Indonesian territory. This research compared the abundance of microplastics i Cymodocea rotundata seagrass, sediment, and water on Rambut Island, Seribu Islands, Jakarta, in 2022 and 2023. Samples were taken as many as eight individuals of seagrass per station, sediment, and water by random sampling method.  The seagrass samples were cut 2 cm long and scraped, the sediment was dried and then given a saturated solution of NaCl, and the water sample was given NaCl. Then all samples were observed under a microscope. The average microplastic abundance of C. rotundata seagrass leaves in 2022 was 42 particles/cm and in 2023, it was 4.46 particles/cm. The average abundance of microplastics in sediments in 2022 was 73.53 particles/g and in 2023, it was 79.56 particles/g. The abundance of microplastics in water samples in 2022 was 51.33 particles/L and in 2023, it was 53.78 particles/L. The Spearman Correlation Test explains that the microplastic abundance of seagrass with sediment, water with sediment, and seagrass with water samples had a strong correlation. The results of the T-test stated that there was no significant difference in the abundance of microplastics in seagrass leaves of C. rotundata (sig. (2-tailed) 0.182>0.05) and there was a significant difference in the abundance of microplastics in sediments in 2022 and 2023 (sig. (2-tailed) 0.007<0.05). The results of the ATR-FTIR test found the polymer content of CA, ABS, HDPE, PMMA, PVC, and PET in C. rotundata seagrass samples."