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"Penelitian ini bertujuan untuk mengetahui kelimpahan mikroplastik pada air, sedimen, serta insang dan saluran pencernaan ikan mujair Oreochromis mossambicus (Peters, 1852) dan Ikan Sepat Rawa Trichopodus trichopterus (Pallas, 1770) di Situ Mahoni, Kampus Universitas Indonesia, Depok. Sampel air diambil sebanyak 20 L menggunakan beaker glass 1000 mL lalu disaring menggunakan plankton net hingga tertampung volume air 300 mL, sampel sedimen diambil menggunakan ekman grab dan ditampung pada jar kaca 250 mL. Sementara itu, sampel ikan mujair dan ikan sepat rawa masing-masing diambil 15 ekor dengan cast net lalu disimpan pada wadah penyimpanan berisi alkohol 70%. Ekstraksi sampel dilakukan di Laboratorium Biologi Kelautan, Departemen Biologi, FMIPA UI dengan metode penghancuran oleh HNO3 65% untuk sampel ikan. Sedangkan, sampel sedimen dikeringkan dengan menggunakan oven selama 2 hari. Selanjutnya, seluruh sampel dicampurkan dengan larutan NaCl jenuh untuk mengapungkan mikroplastik. Kemudian dilakukan analisis kelimpahan, bentuk, dan warna mikroplastik menggunakan mikroskop. Hasil penelitian menunjukkan rata-rata total kelimpahan mikroplastik pada air diperoleh 437,67 ± 30,21 partikel L-1, pada sedimen diperoleh 36.237,04 ± 16.702,60 partikel kg-1, pada insang ikan mujair diperoleh 785,78 ± 292,07 partikel ind-1, pada insang ikan sepat rawa diperoleh 553,33 ± 242,54 partikel ind-1, saluran pencernaan ikan mujair diperoleh 1.058,67 ± 215,44 partikel ind-1, pada saluran pencernaan ikan sepat rawa diperoleh 892,89 ± 156,52 partikel ind-1. Secara keseluruhan, bentuk mikroplastik yang paling banyak ditemukan adalah mikroplastik berbentuk fiber. Berdasarkan hasil uji Mann-Whitney terhadap sampel insang dan saluran pencernaan dari ikan mujair dan ikan sepat rawa didapatkan bahwa terdapat perbedaan jumlah mikroplastik yang signifikan.

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The purpose of this study is to identify the abundance of microplastics in water, sediment, gills, and digestive tract of tilapia Oreochromis mossambicus (Peters,1852) and three spot gourami Trichopodus trichopterus (Pallas, 1770) in Situ Mahoni, University of Indonesia Campus, Depok. As much as 20 Litres of water samples were taken using a 1000 mL beaker glass and then filtered using a plankton net until 300 mL of water is stored, Sediment samples were taken using an ekman grab then stored on a 250 mL glass jar, while 15 samples of the tilapia and three spot gourami were caught using a cast net and then stored with alcohol 70%. Sample extractions were carried out at the Marine Biology Laboratory, Department of Biology, FMIPA UI by pulverizing the fish samples with HNO3 65%. The sediment samples were dried using the oven for 2 days. Then, all of the samples were mixed with a NaCl solution to float the microplastics. Then, an analysis of abundance, shape, and colours of microplastics under the microscope were done. The results showed that the average total abundance of microplastics was 437,67 ± 30,21 L-1 particles in water samples, in sediment samples was 36.237,04 ± 16.702,60 kg-1 particles in sediment samples, 785,78 ± 292,07 ind-1 particles in the gills of tilapia, 553,33 ± 242,54 ind-1 particles in the gills of the three spot gourami, 1.058,67 ± 215,44 ind-1 particles in the digestive tract of the tilapia, 892,89 ± 156,52 ind-1 particles in the digestive tract of the three spot gourami. Overall, the predominant form of microplastic in the water, sediment, gills, and digestive tract is in the form of fiber. There is a significant difference between the abundance of microplastics based on the results of the Mann-Whitney Test on gill and digestive tract samples of tilapia and three spot gourami."

"Keberadaan mikroplastik di perairan dapat mengancam biota di dalamnya. Penelitian dilakukan dengan tujuan menganalisis kelimpahan, bentuk, dan warna mikroplastik pada air, sedimen, insang dan saluran pencernaan ikan sapu-sapu Pterygoplichthys pardalis dari Situ Kenanga dan Situ Mahoni, Kampus Universitas Indonesia, Depok. Pengambilan sampel air dan sedimen dilakukan pada inlet, midlet, dan outlet kedua situ. Sampel air disaring menggunakan plankton net, sampel sedimen diambil menggunakan Ekman grab kemudian dikeringkan menggunakan oven, sampel P. pardalis sebanyak 15 individu diambil menggunakan cast net dari masing-masing situ, kemudian insang dan saluran pencernaannya diisolasi dan didestruksi menggunakan HNO3 65%. Tiap sampel yang diperoleh dilarutkan dengan larutan NaCl jenuh agar terjadi flotasi. Pengamatan dilakukan menggunakan mikroskop cahaya dan Sedgwick Rafter Chamber untuk meletakkan sampel, dengan mengamati bentuk, warna, dan jumlah partikel mikroplastik. Hasil penelitian menunjukkan bahwa bentuk fiber, film, fragmen, granula, serta warna transparan, hitam, biru, dan merah muda ditemukan pada semua sampel. Kelimpahan mikroplastik di Situ Kenanga pada air sebanyak 48,26 ± 23,51 partikel L-1, sedimen 45837,04 ± 36305,97 partikel Kg-1, insang 290,48 ± 154,58 partikel g-1 atau 1156,44 ± 378,69 partikel ind-1, saluran pencernaan 134,37 ± 55,72 partikel g-1 atau 1364,89 ± 339,54 partikel ind-1. Kelimpahan mikroplastik di Situ Mahoni pada air sebanyak 48,63 ± 30,21 partikel L-1, sedimen 36237,04 ± 16702,60 partikel Kg-1, insang 287,23 ± 109,40 partikel g-1 atau 1153,78 ± 324,32 partikel ind-1, saluran pencernaan 123,77 ± 34,35 partikel g-1 atau 1304,44 ± 270,90 partikel ind-1. Tidak terdapat perbedaan signifikan antara kelimpahan mikroplastik di Situ Kenanga dan Situ Mahoni pada semua sampel.

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The presence of microplastics in the water could threaten the biota there. This study was conducted to analyze the abundance, shapes, and colors of microplastics in water, sediment, gills and digestive tract of amazon sailfin catfish Pterygoplichthys pardalis from Situ Kenanga and Situ Mahoni, Universitas Indonesia Campus, Depok. Sampling of water and sediment were carried out at the inlet, midlet, and outlet of both situ. Water samples were filtered using plankton net, sediment samples were taken using Ekman grab and dried using an oven, as many as 15 individual P. pardalis samples were taken using cast net from each situ, then their gills and digestive tract were isolated and pulverized using 65% HNO3. Each sample obtained was dissolved with saturated NaCl solution for flotation to occur. Observations were made using a light microscope and Sedgwick Rafter Chamber to place each sample, by observing the shape, color, and number of microplastic particles. The results showed that the shapes of fibers, films, fragments, granules, as well as transparent, black, blue, and pink colors were found in all samples. The abundance of microplastics in Situ Kenanga water was 48.26 ± 23.51 particles L-1, sediment 45837.04 ± 36305.97 particles Kg-1, gills 290.48 ± 154.58 particles g-1 or 1156, 44 ± 378.69 ind-1 particles, digestive tract 134.37 ± 55.72 particles g-1 or 1364.89 ± 339.54 ind-1 particles. The abundance of microplastics in Situ Mahoni water was 48.63 ± 30.21 particles L-1, sediment 36237.04 ± 16702.60 particles Kg-1, gills 287.23 ± 109.40 particles g-1 or 1153, 78 ± 324.32 ind-1 particles, digestive tract 123.77 ± 34.35 particles g-1 or 1304.44 ± 270.90 ind-1 particles. There was no significant difference between the abundance of microplastics in Situ Kenanga and Situ Mahoni in all samples."

"Mikroplastik yang mencemari perairan tawar dapat mengganggu keberlangsungan hidup biota di dalamnya. Penelitian ini bertujuan untuk menganalisis bentuk, warna, dan kelimpahan mikroplastik yang terkandung pada air, sedimen, insang, dan saluran pencernaan ikan red devil Amphilophus labiatus di Situ Kenanga dan Situ Mahoni, Universitas Indonesia, Depok, Jawa Barat. Sampel air dan sedimen di ambil pada 3 stasiun pengambilan sampel dari kedua situ. Sampel ikan red devil diperoleh 15 ekor pada masing – masing situ. Sampel insang dan saluran pencernaan dihancurkan menggunakan larutan asam nitrat (HNO3) 65%. Sampel air diambil 20 L dan disaring menggunakan plankton net. Sampel sedimen dikeringkan dalam oven sebanyak 25 gram. Larutan NaCl jenuh digunakan untuk memisahkan mikroplastik dengan pengotor. Sebanyak 1 mL sample diteteskan pada Sedgwick Rafter Chamber kemudian diamati dan dihitung berdasarkan bentuk mikroplastik di bawah mikroskop. Hasil penelitian menunjukkan kelimpahan mikroplastik bentuk fiber, fragmen, film, granula terdapat pada seluruh sampel dengan warna yang beragam. Hasil penelitian menunjukkan total kelimpahan rata – rata mikroplastik di Situ Kenanga sebanyak 434,33 ± 23,51 partikel L-1 pada air, 45.837,04 ± 36.305,97 partikel kg-1 pada sedimen, 268,33 ± 119,18 partikel gr-1 dan 1266,2 ± 349,72 partikel ind-1 pada insang, 287,79 ± 185,22 partikel gr-1 dan 978,22 ± 336,38 partikel ind-1 pada saluran pencernaan. Pada Situ Mahoni sebesar 437,67 ± 30,21 partikel L-1 pada air, 36.237,04 ± 16.702,59 partikel kg-1 pada sedimen, 429,18 ± 187,50 partikel gr-1 dan 1233,8 ± 253,60 partikel ind-1 pada insang, 318,04 ± 114,94 partikel gr-1 dan 1053,78 ± 328,44 partikel ind-1 pada saluran pencernaan.

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Microplastics that contaminate freshwater can disrupt the survival of the biota in it. This study aims to analyze the shape, color, and abundance of microplastics contained in water, sediment, gills, and digestive tract of red devil fish Amphilophus labiatus in Situ Kenanga and Situ Mahoni, University of Indonesia, Depok, West Java. Water and sediment samples were taken at 3 sampling stations from the two Situ. Samples of red devil fish were obtained 15 tails in each Situ. Samples of gills and digestive tract were destroyed using 65% nitric acid (HNO3) solution. Water samples were taken 20 L and filtered using a plankton net. Sediment samples were dried in an oven as much as 25 grams. Saturated NaCl solution was used to separate microplastics with impurities. A total of 1 mL of the sample was dropped into the Sedgwick Rafter Chamber and then observed and calculated based on the shape of the microplastic under a microscope. The results showed an abundance of microplastics in the form of fibers, fragments, films, and granules in all samples with various colors. The results showed the total average abundance of microplastics in Situ Kenanga was 434,33 ± 23,51 L-1 particles in water, 45.837,04 ± 36.305,97 particles kg-1 in sediments, 268.33 ± 119.18 particles gr-1 and 1266.2 ± 349.72 particles ind-1 in the gills, 287.79 ± 185.22 particles gr-1 and 978.22 ± 336.38 particles ind-1 in the digestive tract. At Situ Mahoni there are 437,67 ± 30,21 particles L-1 in water, 36.237,04 ± 16.702,59 particles kg-1 in sediments, 429.18 ± 187.50 particles gr-1 and 1233.8 ± 253.60 particles ind-1 on the gills, 318.04 ± 114.94 particles gr-1 and 1053.78 ± 328.44 particles ind-1 in the digestive tract.

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"Telah dilakukan penelitian yang bertujuan untuk menganalisis bentuk dan kelimpahan mikroplastik pada air, insang, dan saluran pencernaan ikan mujair Oreochromis mossambicus dan ikan setan merah Amphilophus labiatus yang terdapat di Situ Salam, Kampus Universitas Indonesia, Depok. Sampel air diambil sebanyak 20 L lalu disaring menggunakan plankton net sampai volume air menjadi 200 ml sementara sampel ikan mujair dan ikan setan merah diambil masing-masing 10 ekor dengan jala lalu disimpan di wadah penyimpanan berisi formalin 40%. Ekstraksi sampel dilakukan di Laboratorium Biologi Kelautan, Departemen Biologi, FMIPA UI dengan metode dekstruksi oleh HNO3 65% kemudian dilakukan analisis bentuk dan kelimpahan mikroplastik di bawah mikroskop. Hasil penelitian menunjukkan, rata-rata total kelimpahan mikroplastik pada air diperoleh 153,7 ± 44,2 partikel L-1, pada saluran pencernaan ikan mujair diperoleh 2.868 ± 723,5 partikel ind-1, pada saluran pencernaan ikan setan merah diperoleh 3.548,4 ± 1031,4 partikel ind-1, pada insang ikan mujair diperoleh 3.782,6 ± 1.171,6 partikel ind-1, dan pada insang ikan setan merah diperoleh 3.848 ± 863,1 partikel ind-1. Bentuk mikroplastik yang mendominasi pada air situ, saluran pencernaan, dan insang adalah bentuk fiber. Berdasarkan hasil Uji T Dua Sampel terhadap sampel insang dan pencernaan dari ikan mujair dan ikan setan merah menunjukkan tidak ada perbedaan signifikan.

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Research has been carried out that aims to analyze the shape and microplastics in the water, gills, and digestive tract of tilapia fish Oreochromis mossambicus and red devil fish Amphilophus labiatus that found in Salam Lake, University of Indonesia Campus, Depok. Water samples were taken as much as 20 L and then filtered using a plankton net until the water volume became 200 ml while samples of tilapia fish and red devil fish were taken each with a net and stored in a storage container containing formalin 40%. Sample extraction was carried out at the Marine Biology Laboratory, Department of Biology, FMIPA UI with the destruction method by HNO3 65% and then analyzed the shape and abundance of microplastics under a microscope. The results showed that the average total abundance of microplastics in water was 153,7 ± 44,2 L-1 particles, in the digestive tract of tilapia fish it was obtained 2.868 ± 723,5 ind-1 particles, in the digestive tract of red devil fish obtained 3.548,4 ± 1.031,4 ind-1 particles, in the gills of tilapia fish obtained 3.782,6 ± 1.171,6 ind-1 particles, and in the gills of red devil fish obtained 3.848 ± 863,1 particles ind-1. The predominant form of microplastic in the water, digestive tract, and gills is the form of fiber. Based on the results of the Two-Sample T-Test on gill and digestive samples of tilapia fish and red devil fish, there is no significant difference."

"Mikroplastik diketahui telah tertelan oleh berbagai taksa biota. Penelitian ini dilakukan untuk menganalisis kelimpahan, bentuk, dan warna mikroplastik yang terdapat pada air, sedimen, dan ikan cere Gambusia affinis di Situ Kenanga dan Situ Mahoni, Universitas Indonesia, Depok, Jawa Barat. Seluruh sampel diambil di 3 stasiun yaitu inlet, midlet, dan outlet. Sampel air 20 L disaring menggunakan plankton net 350 mesh, sampel sedimen diambil 250 mL menggunakan Ekman grab, ikan cere diambil secara acak menggunakan dip net sebanyak 20 individu dari tiap situ. Sampel ikan diawetkan dengan alkohol 70% dan diekstraksi menggunakan 10 mL NaOH 1 M. Sedimen dikeringkan pada oven 65°C dan dihaluskan. Seluruh sampel dilarutkan dengan NaCl jenuh. Larutan dihomogenisasi sebanyak 20 mL dan 1 mL diteteskan Sedgewick Rafter Chamber untuk diamati di bawah mikroskop cahaya. Hasil pengamatan menunjukkan total rata-rata kelimpahan mikroplastik di Situ Kenanga pada air sejumlah 434,33 ± 23,51 partikel L-1, pada sedimen sejumlah 45.837,04 ± 36.305,97 partikel kg-1, pada ikan cere sejumlah 287, 67 ± 95,65 partikel ind-1 atau 212,28 ± 61,88 partikel gram-1. Sementara itu, pada air di Situ Mahoni sejumlah 437,67 ± 30,21 partikel L-1, pada sedimen sejumlah 36.237,04 ± 16.702,59 partikel kg-1, dan pada ikan cere sejumlah 275 ± 95,82 partikel ind-1 atau 654,28 ± 279,71 partikel gram-1. Hasil analisis Uji T Dua Sampel menunjukkan tidak terdapat perbedaan nyata pada sampel air, sedimen, dan ikan cere (partikel ind-1), tetapi terdapat perbedaan nyata pada ikan cere (partikel g-1) dari kedua situ. Fiber dan warna hitam merupakan bentuk dan warna mikroplastik yang mendominasi keseluruhan sampel.

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Microplastics have been ingested by various biota taxa. This study was aimed to analyze the abundance, shape, and color of microplastic found in water, sediment, and mosquitofish Gambusia affinis in Situ Kenanga and Situ Mahoni, University of Indonesia, Depok, West Java. All samples were taken at 3 stations, namely inlet, midlet, and outlet. Water samples (20 L) were filtered using a 350 mesh plankton net, sediment samples (250 mL) were taken using an Ekman grab, mosquitofish were taken randomly using a dip net, and 20 individuals from each situ (total of 40 individuals). Fish samples were preserved in 70% alcohol and extracted using 10 mL of 1 M NaOH. Sediments were dried in an oven at 65°C and pulverized. All samples were dissolved with saturated NaCl. The solution (20 mL) was homogenized, then 1 mL of solution was taken to the Sedgewick Rafter Chamber and then observed under a light microscope. The results showed that the average total of microplastics in Situ Kenanga in water was 434,33 ± 23,51 L-1 particles, in sediments were 45.837,04 ± 36.305,97 kg-1 particles, and in fish were 287, 67 ± 95,65 ind-1 particles or 212,28 ± 61,88 gram-1 particles. Meanwhile, the average total of microplastics in Situ Mahoni in water were 437,67 ± 30,21 L-1 particles, in sediments were 36.237,04 ± 16.702,59 kg-1 particles, and in fish were 275 ± 95,82 ind-1 particles or 654,28 ± 279,71 gram-1 particles. The results of the Two Sample T-Test analysis showed that there was no difference in water, sediment, and mosquitofish (ind-1 particles) between the two situ, but there was a significant difference in mosquitofish (g-1 particles) from the two situ. Fiber and black were respectively the predominant shapes and colors of microplastics in all samples."

"Mikroplastik didefinisikan sebagai partikel plastik dengan ukuran <5 mm. Mikroplastik dapat masuk ke dalam tubuh organisme air khususnya ikan melalui oral dan insang. Penelitian ini bertujuan untuk mengetahui bentuk dan kelimpahan mikroplastik pada air, insang dan saluran pencernaan ikan mujair Oreochromis mossambicus (Peters, 1852) serta menguji perbedaan kelimpahan mikroplastik pada ikan mujair di Danau Kenanga dan Danau Agathis Universitas Indonesia. Penelitian dilakukan di Departemen Biologi FMIPA UI, Depok. Sampel ikan mujair diperoleh sebanyak total 20 ekor dari kedua danau dengan panjang tubuh relatif antara 16--19 cm. Insang dan saluran pencernaan dari 20 sampel ikan mujair diisolasi, kemudian diekstraksi dan dihancurkan menggunakan larutan asam nitrat (HNO3) 65%. Sampel yang telah diekstraksi kemudian diberi larutan NaCl jenuh agar mikroplastik mengapung ke permukaan. Sampel diteteskan sebanyak 1 ml pada Sedgwick Rafter Chamber kemudian diamati di bawah mikroskop dan dihitung jumlah mikroplastik berdasarkan bentuk partikelnya. Total kelimpahan rata-rata mikroplastik pada air Danau Kenanga sebesar 1.766,6 ± 40,11 partikel/L, sementara total kelimpahan rata-rata mikroplastik air pada Danau Agathis sebesar 1.885,53 ± 106,27 partikel/L. Total kelimpahan rata-rata mikroplastik ikan mujair pada Danau Kenanga di insang sebanyak 6.232 ± 1.898,66 partikel/ind dan di saluran pencernaan sebanyak 9.108 ± 4.027,14 partikel/ind, sementara total kelimpahan rata-rata mikroplastik ikan mujair pada Danau Agathis di insang sebanyak 6.716 ± 2.467,67 partikel/ind dan di saluran pencernaan sebanyak 4.038 ± 2.180,75 partikel/ind. Persentase komposisi bentuk mikroplastik yang ditemukan dominan pada Danau Kenanga terdapat bentuk fragmen sebesar 40% di air; fiber 80% di insang dan fiber 75% di saluran pencernaan, Sementara komposisi bentuk mikroplastik yang ditemukan dominan pada Danau Agathis terdapat bentuk fiber sebesar 43% di air; 75% di insang dan 67% di saluran pencernaan. Hasil analisis statistik Uji Mann-Whittney menunjukkan adanya perbedaan secara signifikan kelimpahan mikroplastik pada ikan mujair di Danau Kenanga dan Danau Agathis, Universitas Indonesia.

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Microplastics are define as plastic particles with a size of <5 mm. Microplastics can enter the body of aquatic organisms, especially fish through the mouth and gills. This study aims to determine the shape and abundance of microplastics in water, gill and digestive tract tilapia fish Oreochromis mossambicus (Peters, 1852) at Kenanga Pond and Agathis Pond, University of Indonesia and to analyze and examine differences in the abundance of microplastics in tilapia fish at Kenanga Pond and Agathis Pond, University of Indonesia. The study was conducted at Department of Biology FMIPA UI, Depok. Total 20 tilapia fish from the two lakes with relative length between 16--19 cm. Gills and digestive tract samples were obtained of tilapia fish were isolated, extracted and then crushed using a saturated solution of nitric acid (HNO3) 65%. The extracted sample is then given NaCl solution so that the microplastic floats to the surface. As much as 1 ml NaCl were dropped in the Sedgwick Rafter Chamber then observed under a microscope. The number of microplastic was calculated based on the type. The average total abundance of microplastics in the water of Kenanga Pond was 1.766,6 ± 40,11 particles/L, meanwhile the average total abundance of microplastics in the water of Agathis Pond was 1.885,53 ± 106,27 particles/L. The average total microplastics in tilapia gill at Kenanga Pond was 6.232 ± 1.898,66 particles/ind and in the digestive tract was 9.108 ± 4,027.14 particles/ind, meanwhile the average total abundance microplastics in tilapia gill at Agathis Pond was 6.716 ± 2.467,67 particles/ind and in the digestive tract was 4.038 ± 2.180,75 particles/ind. The percentage composition of the microplastic form that was found dominant at Kenanga Pond were as much as 40% fragments in the water; 80% fiber in the gills and 75% fiber in the digestive tract. Meanwhile, the percentage composition of the microplastic form that was found dominant at Agathis Pond were as much as 43% fiber in the water; 75% in the gills and 67% in the digestive tract. The results of the Mann-Whittney test statistical analysis showed that there was significant difference between abundance of microplastics in tilapia fish at of Kenanga Pond and Agathis Pond, University of Indonesia."

"Peningkatan jumlah limbah plastik yang dibuang ke Situ Rawa Besar mengancam habitat ikan cere. Limbah plastik akan terdegradasi menjadi partikel kecil yang disebut dengan mikroplastik. Mikroplastik dapat terkonsumsi oleh ikan cere karena ukurannya yang mirip dengan sumber makanannya. Penelitian ini bertujuan untuk mengetahui adanya keberadaan mikroplastik dan menganalisis berbagai macam bentuk mikroplastik pada air, sedimen dan ikan cere Gambusia affinis (Baird & Girard, 1853) di Situ Rawa Besar pada musim kemarau 2022 dan musim hujan 2023. Seluruh sampel diambil di 3 stasiun yaitu inlet, midlet, dan outlet. Sampel air 20 L disaring menggunakan plankton net 350 mesh, sampel sedimen diambil 250 mL menggunakan Ekman grab, ikan cere diambil secara acak menggunakan dip net sebanyak 15 individu. Sampel ikan diawetkan dengan alkohol 70% dan diekstraksi menggunakan 5mL HNO3 1 M. Sedimen dikeringkan pada oven 65C dan dihaluskan. Seluruh sampel dilarutkan dengan NaCl jenuh. Larutan dihomogenisasi sebanyak 20 mL dan 1 mL diteteskan pada Sedgewick Rafter Chamber untuk diamati di bawah mikroskop cahaya. Hasil pengamatan pada musim kemarau 2022 menunjukkan total rata-rata kelimpahan mikroplastik pada air sejumlah 46,33 ± 4,67 partikel L-1, pada sedimen sejumlah 31.777,78 ± 16.344,67 partikel kg-1, pada ikan cere sejumlah 291,11 ± 73,10 partikel ind-1 atau 93,52 ± 14,33 partikel cm-1. Sementara itu, pada musim hujan 2023 menunjukkan total rata-rata kelimpahan mikroplastik pada air sejumlah 64,67 ± 8,70 partikel L-1 , pada sedimen sejumlah 34.222,22 ± 17.493,91 partikel kg-1, dan pada ikan cere sejumlah 254,00 ± 40,28 partikel ind-1 atau 89,71 ± 12,15 partikel cm-1. Hasil Uji Korelasi Spearman menunjukkan terdapat korelasi positif sangat kuat antara kelimpahan mikroplastik pada air dengan sedimen, serta antara panjang ikan cere dengan kelimpahan mikroplastik pada ikan cere. Hasil analisis Uji T Dua Sampel menunjukkan tidak terdapat perbedaan nyata pada seluruh sampel.

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The increasing amount of plastic waste dumped into Situ Rawa Besar threatens the mosquitofish habitat. Plastic waste degrades into small particles called microplastics. Microplastics can be consumed by mosquitofish because their size is similar to that of their food source. This research aims to determine the presence of microplastics and analyze various forms of microplastics in water, sediment, and the mosquitofish Gambusia affinis (Baird & Girard, 1853) in Situ Rawa Besar during the 2022 dry season and the 2023 wet season. All samples were taken at three stations, namely inlet, midlet, and outlet. A 20 L water sample was filtered using a 350 mesh plankton net, 250 mL sediment samples were taken using an Ekman grab, and 15 mosquitofish were randomly captured using a dip net. Fish samples were preserved with 70% alcohol and extracted using 5 mL of 1 M HNO3. The sediment was dried in an oven at 65°C and ground. All samples were dissolved with saturated NaCl. The solution was homogenized to 20 mL, and 1 mL was dropped into the Sedgewick Rafter Chamber to be observed under a light microscope. The results of observations in the 2022 dry season showed that the total average abundance of microplastics in the water was 46.33 ± 4.67 particles L-1, in sediment was 31,777.78 ± 16,344.67 particles kg-1, and in mosquitofish was 291.11 ± 73.10 particles ind-1 or 93.52 ± 14.33 particles cm-1. Meanwhile, in the 2023 wet season, the total average abundance of microplastics in the water was 64.67 ± 8.70 particles L-1, in the sediment was 34,222.22 ± 17,493.91 particles kg-1, and in mosquitofish was 254.00 ± 40.28 particles ind-1 or 89.71 ± 12.15 particles cm-1. The results of the Spearman Correlation Test showed that there was a very strong positive correlation between the abundance of microplastics in water and sediment, as well as between the length of mosquitofish and the abundance of microplastics in mosquitofish. The results of the Two Sample T Test analysis show that there are no significant differences in all samples."

"Penelitian ini menganalisis bentuk dan kelimpahan mikroplastik pada sumber air laut, sumber air tawar petak A, air kolam 19, pakan buatan (pelet), saluran pencernaan, dan feses udang vannamei Litopenaeus vannamei (Boone, 1931) di Tambak Udang Vannamei Unit Sobo, Banyuwangi, Jawa Timur. Sampel air diambil dan disimpan dalam botol kaca sebanyak 300 ml, sampel pakan menggunakan pakan jenis SGH2 buatan perusahaan sendiri sebanyak 10 gr, sedangkan sampel udang digunakan sebanyak 5 individu dari kolam 19 dan tiap udang diambil bagian saluran pencernaan dan fesesnya. Sampel air, pakan, saluran pencernaan, dan feses diamati menggunakan mikroskop serta dilakukan pengulangan sebanyak 3 kali. Sampel diambil pada tanggal 25 Oktober 2021 dengan DOC (Day of Culture) 53 dan penelitian berlangsung selama 1 bulan dari bulan Oktober hingga November 2021. Penelitian memperlihatkan tiga bentuk mikroplastik, yaitu fragmen, film, dan fiber. Mikroplastik bentuk fragmen mendominasi dalam penelitian ini. Selain itu, berdasarkan data kelimpahan mikroplastik, diketahui bahwa mikroplastik pada pakan banyak yang tidak ditemukan ke dalam proses pencernaan. Kemudian, diketahui juga tidak terdapat perbedaan nilai kelimpahan, serta terdapat hubungan kelimpahan mikroplastik pada saluran pencernaan dan feses udang vannamei.

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This study analyzed the shape and abundance of microplastics in seawater sources, plot A freshwater source, pond water, artificial feed, digestive tract, and feces of Litopenaeus vannamei (Boone, 1931) at Unit Sobo Shrimp Ponds, Banyuwangi, East Java. As much as 300 ml of water were taken and stored in glass bottles; feed samples made by the company itself, were taken as much as 10 grams; while shrimp samples were used as many as 5 individuals from 19 ponds, taken from the digestive tract and feces. Each sample were observed using a microscope and repeated 3 times. Samples were taken on October 25, 2021 with DOC (Day of Culture) 53 and the study lasted for 1 month from October to November 2021. Research shows three forms of microplastics, namely fragments, films, and fibers. Microplastic fragments dominate in this study. In addition, based on data on the abundance of microplastics, it is known that many microplastics in feed are not found in the digestive process. Then, it was also known that there was no difference in the abundance value and that there was a relationship between the abundance of microplastics in the digestive tract and feces."

"Penelitian ini bertujuan untuk mendeskripsikan lokasi, jenis, dan kisaran ukuran mikroplastik yang terdapat pada insang kerang hijau Perna viridis. Penelitian ini melanjutkan hasil penelitian Fathonia (2017) mengenai kelimpahan mikroplastik pada kerang hijau dari kolam kerang hijau Kamal Muara, Jakarta Utara. Sampel kerang hijau sebanyak 10 ekor dengan ukuran sekitar 7cm. Insang kerang hijau kemudian diisolasi, baik bagian luar maupun bagian dalam dan dibuat preparat. Preparat insang tersebut kemudian ditandai pada bagian-bagian insang yang dibagi menjadi bagian posterior, anterior, proksimal, dan distal. Preparat kemudian diamati di bawah mikroskop optik cahaya. Partikel mikroplastik yang diamati dicatat posisi dan jenis mikroplastik yang ada di insang kemudian diukur menggunakan aplikasi LAZ EZ. Hasil penelitian menunjukkan bahwa bagian distal mengandung lebih banyak mikroplastik daripada bagian proksimal. Kelompok mikroplastik yang dominan terdapat pada insang kerang hijau adalah jenis serat sebanyak 44% dari total jumlah partikel yang ditemukan. Kisaran ukuran mikroplastik yang ditemukan adalah 20-4500 m.

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This study aims to describe the location, type, and size range of microplastics found in the gills of the green mussel Perna viridis. This study continues the results of Fathonia's research (2017) regarding the abundance of microplastics in green mussels from the green mussel pond of Kamal Muara, North Jakarta. Samples of green mussels as many as 10 tails with a size of about 7cm. The green mussel gills were then isolated, both externally and internally and made preparations. The gill preparations were then marked on the parts of the gills which were divided into posterior, anterior, proximal, and distal parts. The preparations were then observed under a light optical microscope. The observed microplastic particles were recorded and the position and type of microplastic present in the gills were then measured using the LAZ EZ application. The results showed that the distal part contained more microplastics than the proximal part. The dominant group of microplastics found in the gills of green mussels is the type of fiber as much as 44% of the total number of particles found. The size range of microplastics found is 20-4500 m."

"Penelitian ini bertujuan untuk menganalisis kelimpahan serta jenis mikroplastik yang terakumulasi pada air, sedimen, dan saluran pencernaan bulu babi Diadema setosum, menganalisis korelasi anatara kelimpahan mikroplastik yang terkandung pada saluran pencernaan dan sedimen, membandingkan hasil kelimpahan mikroplastik di Pulau Tidung dan Pulau Untung Jawa, Jakarta Kepulauan Seribu. Pengambilan sampel bulu babi Diadema setosum, air, dan sedimen dilakukan dari 15 lokasi yang berbeda berdasarkan letak koloni biota. Sampel saluran pencernaan bulu babi dianalisis dengan cara melarutkannya pada HNO3 65%, sampel air (20 l) disaring dengan plankton net 300 µm, dan sampel sedimen (200 g) dikeringkan di oven terlebih dahulu, kemudian masing-masing sampel diberi NaCl untuk dijenuhkan agar mikroplastik mengapung ke atas permukaan. Masing-masing sampel (1 ml) diletakan pada Sedgwick Rafter Chamber untuk diamati di bawah mikroskop dan dihitung berdasarkan jenis partikel yang ditemui yaitu fiber, film, fragmen, dan granula. Hasil penelitian menunjukan kelimpahan mikroplastik pada Pulau Untung Jawa lebih tinggi sejumlah 99,88 ± 1,79 partikel L-1 pada air, 110.737,77 ± 4.197,61 partikel Kg-1 pada sedimen, dan 2.175,55 ± 584,26 partikel Ind-1 pada saluran pencernaan bulu babi. Pada Pulau Tidung kelimpahan mikroplastik yang terkandung pada air sejumlah 87,4 ± 9,61 partikel L-1, pada sedimen sebesar 87.626,66 ± 4.957,00 partikel Kg-1, dan pada saluran pencernaan bulu babi sebesar 1.786,66 ± 451,17 partikel Ind-1. Adanya korelasi positif antara jumlah mikroplastik dengan berat sedimen yang terkandung dalam saluran pencernaan bulu babi di Pulau Untung Jawa, dan tidak adanya korelasi positif antara jumlah mirkoplastik dengan berat sedimen yang terkandung dalam saluran pencernaan bulu babi di Pulau Tidung.

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This study aims to analyze the abundance and types of microplastics that accumulate in water, sediments, and digestive tracts of sea urchins Diadema setosum, analyzed the extent of microplastic abundance contained in smelting and sediments, compared the results of microplastic abundance in Untung Jawang Island and Tidung Island, Seribu Islands. Samples of sea urchins Diadema setosum, water, and sediment were taken from 15 different locations based on the location of the biota colony. Samples of the digestive tracts of sea urchins were analyzed by dissolving them at 65% HNO3, water samples (20 l) were filtered with a 300 μm plankton net, and sediment samples (200 g) were dried in the oven first, then each sample was given NaCl to saturate the microplastic to use upward display. Each sample (1 ml) is placed in the Sedgwick Rafter Chamber to be examined under a microscope and calculated based on the type of particles found, namely fibers, film, fragments, and granules. The results showed that the abundance of microplastic in Untung Jawa Island was higher at 99,88 ± 1,79 particles L-1 in water, 110.737,77 ± 4.197,61 Kg-1 particles in sediment, and 2.175,55 ± 584,26 Ind-1 particles in the digestive tract of sea urchins. On Tidung Island microplastic abundance contained in the water amounted to 87,4 ± 9,61 particles L-1, the sediment of 87.626,66 ± 4.957,00 particles Kg-1, and in the digestive tract of sea urchins amounted to 1.786,66 ± 451, 17 particles Ind-1. There is a positive correlation between the amount of microplastics and sediment weight contained in the digestive tract of sea urchins on Untung Jawa Island, and there is no positive correlation between the number of microplastics with the sediment weight contained in the digestive tract of sea urchins on Tidung Island."