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"Telah dilakukan penelitian yang bertujuan untuk membandingkan kelimpahan, bentuk, dan warna mikroplastik yang terkandung pada air, sedimen, dan keong mas Pomacea canaliculata di Situ Kenanga dan Situ Mahoni, Kampus Universitas Indonesia, Depok. Pengambilan sampel air, sedimen dan keong mas Pomacea canaliculata dilakukan di 3 titik lokasi yaitu inlet, midlet, dan outlet. Sampel air (20 l) disaring dengan plankton net 350 mesh, sampel sedimen dimasukkan ke jar 250 ml menggunakan Ekman grab lalu dioven dan dihaluskan. Sampel keong mas dikoleksi sebanyak 20 sampel setiap Situ, kemudian dianalisis dengan melarutkannya pada HNO3 65%, lalu sampel dijenuhkan dengan NaCl agar mikroplastik dapat mengapung ke permukaan. Sampel dihomogenisasi (20 ml) dan selanjutnya 1 ml diletakkan di Sedgewick Rafter Chamber untuk diamati di bawah mikroskop dan dihitung kelimpahan mikroplastik, bentuk dan warnanya. Hasil dari penelitian, kelimpahan mikroplastik di Situ Kenanga sejumlah 434,33± 23,51 partikel L-1 pada air, 45.837,04 ± 36.305,97 partikel Kg-1 pada sedimen dan 1.320,33 ± 533,91 partikel Ind-1 dan 116,19 ± 37,1 partikel pergram Ind-1 pada keong mas. Kelimpahan mikroplasik di Situ Mahoni pada air sejumlah 437,67 ± 30,21 partikel L-1, pada sedimen sejumlah 36.237,04 ± 16.702,59 partikel Kg-1, dan keong mas sejumlah 1.301,67 ± 200,72 partikel Ind-1 dan 148,38 ± 40,00 partikel pergram Ind-1. Hasil uji t dari sampel air, sedimen dan keong mas perindividu tidak terdapat perbedaan, sedangkan keong mas pergram ind-1 terdapat perbedaan. Bentuk mikroplastik yang ditemukan diantaranya adalah Fiber, fragmen, film, dan granula. Warna mikroplastik didominasi oleh hitam dan tidak berwarna.

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A study was conducted to compare the abundance, shape, and color of microplastics contained in water, sediment, and gold snail Pomacea canaliculata in Situ Kenanga and Situ Mahoni, University of Indonesia Campus, Depok. Sampling of water, sediment and gold snail Pomacea canaliculata was carried out at 3 locations, namely inlet, midlet, and outlet. The water sample (20 l) was filtered with a 350 mesh plankton net, the sediment sample was put into a 250 ml jar using an Ekman grab and then baked and mashed. The gold snail samples were collected as many as 20 samples each Situ, then analyzed by dissolving them in 65% HNO3, then the samples were saturated with NaCl so that the microplastics could float to the surface. The saturated sample was homogenized (20 ml) and then 1 ml was placed in the Sedgewick Rafter Chamber to be observed under a microscope and the microplastic abundance, shape and color were calculated. The results of the study, the abundance of microplastics in Situ Kenanga were 434.33 ± 23.51 particles L-1 in water, 45,837.04 ± 36,305.97 particles Kg-1 in sediments and 1,320.33 ± 533.91 particles Ind-1 and 116.19 ± 37.1 particles per gram Ind-1 in gold snails. The abundance of microplastics in Situ Mahoni in water was 437.67 ± 30.21 particles L-1, in sediments was 36.237.04 ± 16.702.59 particles Kg-1, and golden snails were 1,301.67 ± 200.72 Ind-1 particles and 148.38 ± 40.00 particles per gram Ind-1. The results of the t-test of water, sediment and individual gold snails were not different, while the gold snails per gram were different. The forms of microplastics found included fiber, fragments, films, and granules. The color of microplastics is dominated by black and colorless."

"Situ Mahoni dan Situ Kenanga merupakan dua Situ di Universitas Indonesia dengan sumber masukan air yang berbeda. Perbedaan sumber masukan air dapat dapat menjadikan struktur komunitas epizoik di kedua situ berbeda pula tergantung pada parameter fisika-kimia air pada perairan tersebut. Penelitian ini bertujuan untuk mengetahui faktor abiotik yang berpengaruh terhadap struktur komunitas epizoik dan mengetahui struktur komunitas epizoik pada permukaan cangkang Pomacea canaliculata di Situ Mahoni dan Situ Kenanga. Pengambilan sampel dan pengukuran parameter fisika-kimia air dilakukan di Situ Mahoni dan Situ Kenanga pada bulan Januari—Februari 2022. Korelasi antara parameter fisika-kimia air dengan struktur komunitas epizoik diprediksi menggunakan Partial Least Square – Structural Equation Modeling (PLS-SEM). Model yang dihasilkan menunjukkan bahwa terdapat korelasi positif antara parameter fisika-kimia air dengan struktur komunitas epizoik pada kedua situ. Secara keseluruhan, oksigen terlarut, pH, nitrat, dan fosfat berkorelasi lebih kuat dibandingkan dengan korelasi dari suhu, kecerahan, kecepatan arus, dan turbiditas terhadap keanekaragaman dan kemerataan jenis epizoik. Pada Situ Mahoni, ditemukan 23 marga dari 6 kelas epizoik, sedangkan pada Situ Kenanga ditemukan 25 marga dari 7 kelas. Kelimpahan epizoik tertinggi di kedua situ berasal dari kelas Bacillariophyceae.

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Situ Mahoni and Situ Kenanga are two small lakes in University of Indonesia with different sources of water intake. The difference in water sources could make the epizoic community structure in these two sites different depending on the physic-chemical parameters of the water. The aim of this study is to determine the abiotic effects on the epizoic community structure, also to determine the composition and density of epizoic on the shell surface of Pomacea canaliculata in Situ Mahoni and Situ Kenanga. Sampling and measurement of water physic-chemical parameters were carried out in Situ Mahoni and Situ Kenanga in January—February 2022. Correlation between water physico-chemical parameters and epizoic community structure was predicted using Partial Least Square – Structural Equation Modeling (PLS-SEM). The model showed that there was a positive correlation between the physico-chemical parameters of the water and the structure of the epizoic community at both Situ. Overall, dissolved oxygen, pH, nitrate, and phosphate correlated more strongly than the correlations of temperature, transparency, current velocity, and turbidity to the diversity and evenness of epizoic species. There were 23 genera from 6 classes found in Situ Mahoni, and 25 genera from 7 classes in Situ Kenanga. The highest epizoic abundance in both locations was Bacillariophyceae."

"Keberadaan mikroplastik di perairan dapat mengancam biota di dalamnya. Penelitian dilakukan dengan tujuan menganalisis kelimpahan, bentuk, dan warna mikroplastik pada air, sedimen, insang dan saluran pencernaan ikan sapu-sapu Pterygoplichthys pardalis dari Situ Kenanga dan Situ Mahoni, Kampus Universitas Indonesia, Depok. Pengambilan sampel air dan sedimen dilakukan pada inlet, midlet, dan outlet kedua situ. Sampel air disaring menggunakan plankton net, sampel sedimen diambil menggunakan Ekman grab kemudian dikeringkan menggunakan oven, sampel P. pardalis sebanyak 15 individu diambil menggunakan cast net dari masing-masing situ, kemudian insang dan saluran pencernaannya diisolasi dan didestruksi menggunakan HNO3 65%. Tiap sampel yang diperoleh dilarutkan dengan larutan NaCl jenuh agar terjadi flotasi. Pengamatan dilakukan menggunakan mikroskop cahaya dan Sedgwick Rafter Chamber untuk meletakkan sampel, dengan mengamati bentuk, warna, dan jumlah partikel mikroplastik. Hasil penelitian menunjukkan bahwa bentuk fiber, film, fragmen, granula, serta warna transparan, hitam, biru, dan merah muda ditemukan pada semua sampel. Kelimpahan mikroplastik di Situ Kenanga pada air sebanyak 48,26 ± 23,51 partikel L-1, sedimen 45837,04 ± 36305,97 partikel Kg-1, insang 290,48 ± 154,58 partikel g-1 atau 1156,44 ± 378,69 partikel ind-1, saluran pencernaan 134,37 ± 55,72 partikel g-1 atau 1364,89 ± 339,54 partikel ind-1. Kelimpahan mikroplastik di Situ Mahoni pada air sebanyak 48,63 ± 30,21 partikel L-1, sedimen 36237,04 ± 16702,60 partikel Kg-1, insang 287,23 ± 109,40 partikel g-1 atau 1153,78 ± 324,32 partikel ind-1, saluran pencernaan 123,77 ± 34,35 partikel g-1 atau 1304,44 ± 270,90 partikel ind-1. Tidak terdapat perbedaan signifikan antara kelimpahan mikroplastik di Situ Kenanga dan Situ Mahoni pada semua sampel.

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The presence of microplastics in the water could threaten the biota there. This study was conducted to analyze the abundance, shapes, and colors of microplastics in water, sediment, gills and digestive tract of amazon sailfin catfish Pterygoplichthys pardalis from Situ Kenanga and Situ Mahoni, Universitas Indonesia Campus, Depok. Sampling of water and sediment were carried out at the inlet, midlet, and outlet of both situ. Water samples were filtered using plankton net, sediment samples were taken using Ekman grab and dried using an oven, as many as 15 individual P. pardalis samples were taken using cast net from each situ, then their gills and digestive tract were isolated and pulverized using 65% HNO3. Each sample obtained was dissolved with saturated NaCl solution for flotation to occur. Observations were made using a light microscope and Sedgwick Rafter Chamber to place each sample, by observing the shape, color, and number of microplastic particles. The results showed that the shapes of fibers, films, fragments, granules, as well as transparent, black, blue, and pink colors were found in all samples. The abundance of microplastics in Situ Kenanga water was 48.26 ± 23.51 particles L-1, sediment 45837.04 ± 36305.97 particles Kg-1, gills 290.48 ± 154.58 particles g-1 or 1156, 44 ± 378.69 ind-1 particles, digestive tract 134.37 ± 55.72 particles g-1 or 1364.89 ± 339.54 ind-1 particles. The abundance of microplastics in Situ Mahoni water was 48.63 ± 30.21 particles L-1, sediment 36237.04 ± 16702.60 particles Kg-1, gills 287.23 ± 109.40 particles g-1 or 1153, 78 ± 324.32 ind-1 particles, digestive tract 123.77 ± 34.35 particles g-1 or 1304.44 ± 270.90 ind-1 particles. There was no significant difference between the abundance of microplastics in Situ Kenanga and Situ Mahoni in all samples."

"Penelitian ini dilatarbelakangi oleh meningkatnya polusi plastik yang menyebabkan pencemaran mikroplastik di perairan air tawar. Situ Rawa Besar, Depok merupakan salah satu situ yang tercemar oleh mikroplastik akibat aktivitas manusia di sekitarnya. Mikroplastik dapat berdampak negatif bagi organisme akuatik seperti ikan sapu-sapu Pterygoplichthys pardalis yang merupakan spesies invasif dan bioindikator pencemaran mikroplastik di situ. Penelitian ini bertujuan untuk menganalisis kelimpahan, bentuk dan warna mikroplastik pada air, sedimen, insang dan saluran pencernaan ikan sapu-sapu di Situ Rawa Besar serta menguji perbedaan kelimpahan mikroplastik pada tahun 2022 dengan 2023. Penelitian ini menggunakan metode deskriptif kuantitatif untuk menganalisis kelimpahan, bentuk dan warna mikroplastik pada air, sedimen, insang, dan saluran pencernaan sapu-sapu di Situ Rawa Besar. Pengambilan sampel dilakukan di tiga stasiun. Parameter fisika-kimia air seperti suhu, pH, oksigen terlarut, dan kecerahan air diukur secara in situ. Sampel air 20 L disaring menggunakan plankton net dan sampel sedimen diambil menggunakan Ekman grab dan dikeringkan dengan oven, lalu ditambahkan larutan NaCl jenuh. Sampel ikan sapu-sapu diambil sebanyak 15 ekor dengan menggunakan jaring ikan. Sampel insang dan saluran pencernaan ikan dipisahkan dengan menggunakan dissecting set dan dilarutkan dengan HNO3 kemudian ditambahkan larutan NaCl jenuh. Perhitungan partikel mikroplastik dilakukan di bawah mikroskop dan sampel diletakkan pada Sedgwick Rafter Chamber. Mikroplastik dianalisis kelimpahannya dan diklasifikasikan menurut bentuknya (pellet, fiber, film, dan fragmen) serta dihitung persentase komposisi mikroplastik dalam sampel. Hasil penelitian pada tahun 2022 menunjukkan bahwa kelimpahan mikroplastik berkisar antara 12,67 – 20,33 partikel L-1 pada air, 14.400,00-38.400,00 partikel kg-1 pada sedimen, 250,67-386,67 partikel ind-1 pada insang, dan 313,33-369,33 partikel ind-1 pada saluran pencernaan. Kelimpahan mikroplastik tersebut lebih rendah dari kelimpahannya pada tahun 2023, yaitu berkisar antara 14,67-32,00 partikel L-1 pada air, 16.533,33-41.600,00 partikel kg-1 pada sedimen, 333,33-446,67 partikel ind-1 pada insang, dan 429,33-502,67 partikel ind-1 pada saluran pencernaan. Bentuk mikroplastik yang paling dominan adalah fiber pada air dan ikan sapu-sapu, fragmen pada sedimen. Warna mikroplastik yang paling dominan adalah biru pada air dan sedimen, hitam pada insang dan saluran pencernaan ikan sapu-sapu. Kelimpahan mikroplastik pada tahun 2023 lebih tinggi dibandingkan tahun 2022, dengan peningkatan sebesar 15,24% pada insang, 24,25% pada saluran pencernaan, 27,91% pada air, dan 7,12% pada sedimen. Penelitian ini menunjukkan peningkatan jumlah mikroplastik di Situ Rawa Besar seiring berjalannya waktu yang didukung oleh beberapa faktor seperti peningkatan populasi, peningkatan sampah plastik, aktivitas antropogenik, dan curah hujan.

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This research was motivated by the increasing plastic pollution that causes microplastic contamination in freshwater bodies. Situ Rawa Besar, Depok is one of the lakes that is polluted by microplastics due to human activities around it. Microplastics can have negative impacts on aquatic organisms such as Amazon Sailfin Catfish Pterygoplichthys pardalis which is an invasive species and a bioindicator of microplastic pollution in the lake. This research aims to analyze the abundance, shape and color of microplastics in water, sediment, gills and digestive tract of sailfin catfish in Situ Rawa Besar and to test the difference in microplastic abundance between 2022 and 2023. This research uses descriptive quantitative method to analyze the abundance, shape and color of microplastics in water, sediment, gills, and digestive tract of sailfin catfish in Situ Rawa Besar. Sampling was done at three stations. Physico-chemical parameters of water such as temperature, pH, dissolved oxygen, and water clarity were measured in situ. Water samples of 20 L were filtered using plankton net and sediment samples were taken using Ekman grab and dried with oven, then added with saturated NaCl solution. Sailfin catfish samples were taken as many as 15 individuals using fish net. Gill and digestive tract samples were separated using dissecting set and dissolved with HNO3 then added with saturated NaCl solution. Microplastic particle counting was done under microscope and samples were placed on Sedgwick Rafter Chamber. Microplastics were analyzed for their abundance and classified according to their shape (pellet, fiber, film, and fragment) and the percentage composition of microplastics in the sample was calculated. The results of the research in 2022 showed that the abundance of microplastics ranged from 12.67-20.33 particles L-1 in water, 14,400.00-38,400.00 particles kg-1 in sediment, 250.67-386.67 particles ind-1 in gills, and 313.33-369.33 particles ind-1 in digestive tract. The abundance of microplastics was lower than its abundance in 2023, which ranged from 14.67-32.00 particles L-1 in water, 16,533.33-41,600.00 particles kg-1 in sediment, 333.33-446.67 particles ind-1 in gills, and 429.33-502.67 particles ind-1 in digestive tract. The most dominant shape of microplastics was fiber in water and sailfin catfish, fragment in sediment and digestive tract of sailfin catfish. The most dominant color of microplastics was blue in water and sediment, black in gills and digestive tract of sailfin catfish. The abundance of microplastics increased from 2022 to 2023, with an increase of 15.24% in gills, 24.25% in digestive tract, 27.91% in water, and 7.12% in sediment. This research shows an increase in the number of microplastics in Situ Rawa Besar over time supported by several factors such as population growth, increased plastic waste, anthropogenic activities, and rainfall."

"Mikroplastik diketahui telah tertelan oleh berbagai taksa biota. Penelitian ini dilakukan untuk menganalisis kelimpahan, bentuk, dan warna mikroplastik yang terdapat pada air, sedimen, dan ikan cere Gambusia affinis di Situ Kenanga dan Situ Mahoni, Universitas Indonesia, Depok, Jawa Barat. Seluruh sampel diambil di 3 stasiun yaitu inlet, midlet, dan outlet. Sampel air 20 L disaring menggunakan plankton net 350 mesh, sampel sedimen diambil 250 mL menggunakan Ekman grab, ikan cere diambil secara acak menggunakan dip net sebanyak 20 individu dari tiap situ. Sampel ikan diawetkan dengan alkohol 70% dan diekstraksi menggunakan 10 mL NaOH 1 M. Sedimen dikeringkan pada oven 65°C dan dihaluskan. Seluruh sampel dilarutkan dengan NaCl jenuh. Larutan dihomogenisasi sebanyak 20 mL dan 1 mL diteteskan Sedgewick Rafter Chamber untuk diamati di bawah mikroskop cahaya. Hasil pengamatan menunjukkan total rata-rata kelimpahan mikroplastik di Situ Kenanga pada air sejumlah 434,33 ± 23,51 partikel L-1, pada sedimen sejumlah 45.837,04 ± 36.305,97 partikel kg-1, pada ikan cere sejumlah 287, 67 ± 95,65 partikel ind-1 atau 212,28 ± 61,88 partikel gram-1. Sementara itu, pada air di Situ Mahoni sejumlah 437,67 ± 30,21 partikel L-1, pada sedimen sejumlah 36.237,04 ± 16.702,59 partikel kg-1, dan pada ikan cere sejumlah 275 ± 95,82 partikel ind-1 atau 654,28 ± 279,71 partikel gram-1. Hasil analisis Uji T Dua Sampel menunjukkan tidak terdapat perbedaan nyata pada sampel air, sedimen, dan ikan cere (partikel ind-1), tetapi terdapat perbedaan nyata pada ikan cere (partikel g-1) dari kedua situ. Fiber dan warna hitam merupakan bentuk dan warna mikroplastik yang mendominasi keseluruhan sampel.

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Microplastics have been ingested by various biota taxa. This study was aimed to analyze the abundance, shape, and color of microplastic found in water, sediment, and mosquitofish Gambusia affinis in Situ Kenanga and Situ Mahoni, University of Indonesia, Depok, West Java. All samples were taken at 3 stations, namely inlet, midlet, and outlet. Water samples (20 L) were filtered using a 350 mesh plankton net, sediment samples (250 mL) were taken using an Ekman grab, mosquitofish were taken randomly using a dip net, and 20 individuals from each situ (total of 40 individuals). Fish samples were preserved in 70% alcohol and extracted using 10 mL of 1 M NaOH. Sediments were dried in an oven at 65°C and pulverized. All samples were dissolved with saturated NaCl. The solution (20 mL) was homogenized, then 1 mL of solution was taken to the Sedgewick Rafter Chamber and then observed under a light microscope. The results showed that the average total of microplastics in Situ Kenanga in water was 434,33 ± 23,51 L-1 particles, in sediments were 45.837,04 ± 36.305,97 kg-1 particles, and in fish were 287, 67 ± 95,65 ind-1 particles or 212,28 ± 61,88 gram-1 particles. Meanwhile, the average total of microplastics in Situ Mahoni in water were 437,67 ± 30,21 L-1 particles, in sediments were 36.237,04 ± 16.702,59 kg-1 particles, and in fish were 275 ± 95,82 ind-1 particles or 654,28 ± 279,71 gram-1 particles. The results of the Two Sample T-Test analysis showed that there was no difference in water, sediment, and mosquitofish (ind-1 particles) between the two situ, but there was a significant difference in mosquitofish (g-1 particles) from the two situ. Fiber and black were respectively the predominant shapes and colors of microplastics in all samples."

"Peristiwa Pandemi Covid-19 membuat adanya penerapan kebijakan study from home work from home pada Kawasan Universitas Indonesia. Di tahun yang sama, pencemar mikroplastik juga diketahui meningkat di sejumlah badan air. Dari sini, Danau UI yang merupakan rencana sumber air baku bagi Sistem Penyediaan Air Minum di Universitas Indonesia perlu ditinjau kualitas airnya. Oleh karena itu, tujuan dari penelitian ini adalah menganalisis kualitas air Danau UI dengan membandingkan kondisi historis parameter kualitas air Danau UI dari sebelum pandemi hingga saat masa pandemi selama 10 tahun kebelakang. Parameter kualitas air yang ditinjau berupa Suhu, TSS, TDS, pH, DO, BOD, Nitrat, Fosfat, Total Coliform, dan Fecal Coliform. Parameter-parameter tersebut nantinya dapat digunakan untuk membantu menggambarkan status mutu air Danau UI dengan metode STORET. Kemudian, identifikasi pencemar mikroplastik Danau UI disini dilakukan dengan menggunakan larutan jenuh NaCl, Mikroskop Cahaya, serta Sedwigck Rafter Chamber. Hasil dari penelitian menunjukkan adanya sejumlah parameter yang mengalami peningkatan pada masa pandemi, yakni 5 dari 6 Danau di UI mengalami peningkatan nilai DO dengan 50% diantaranya melewati standar baku mutu kelas 1 yakni 6 mg/l, 100% parameter pH pada kondisi netral yakni 7, serta parameter Suhu dan TDS pada Danau UI seluruhnya memenuhi standar baku mutu kelas 1. Kemudian hasil dari penilaian dengan metode STORET pada tahun yang memiliki sejumlah parameter yang lengkap terukur dan dapat dibandingkan menunjukkan kondisi air Danau UI yang buruk. Terlihat pada tahun 2016, 2017, 2018, dan 2019 penilaian STORET Danau UI memiliki nilai yakni -130, -124, -128, dan -128. Hasil tersebut menggambarkan kondisi air Danau UI yang sama dari tahun ke tahunnya pada masa sebelum pandemi hingga saat masa pandemi tahun 2019. Selain itu, mikroplastik yang teridentifikasi disini memiliki rata-rata sebanyak 391,76 partikel/L dengan jenis yang paling dominan berupa fiber sebanyak 64,94% dan microbeads sebanyak 30,79%. Hasil tersebut menunjukkan bahwa Danau UI masih perlu menerapkan sejumlah strategi pengelolaan kualitas air danau.

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The Covid-19 Pandemic event led to the implementation of study-from-home and work-from-home policies in the University of Indonesia Area. In the same year, microplastic contaminants are also known to increase in several water bodies. From here, UI Lakes, which are planned to be the raw water source for Drinking Water Supply System at University of Indonesia, needs to be reviewed for its water quality. Therefore, the purpose of this study is to analyze the water quality of UI Lakes by comparing the historical conditions of UI Lake's water quality parameters from before the pandemic to the time of the pandemic for the past ten years. The water quality parameters reviewed were Temperature, TSS, TDS, pH, DO, BOD, Nitrate, Phosphate, Total Coliform, and Fecal Coliform. These parameters can later be used to help describe the water quality status of UI Lake with the STORET method. Then, the identification of Lake UI microplastic contaminants here was carried out using saturated solution of NaCl, Light Microscope, and Sedwigck Rafter Chamber. The results of the study show that several parameters have increased during the pandemic, namely 5 of 6 lakes in UI experienced an increase in DO values ​​with 50% of them passing the class 1 quality standard, which is 6 mg/l, 100% of the pH parameters in neutral conditions, namely 7, as well as the Temperature and TDS parameters in UI Lakes all meet the class 1 quality standard. Then the results from the STORET method which have parameters that are completely measurable and can be compared show the poor condition of UI Lake's water. It can be seen in 2016, 2017, 2018, and 2019 that the UI Lakes STORET assessment has a value of -130, -124, -128, and -128. These results illustrate the same water conditions at UI Lakes from year to year from the pre-pandemic to the 2019 pandemic. In addition, the microplastics identified here have an average of 391.76 particles/L with the most dominant type being fiber as much as 64.94% and microbeads as much as 30.79%. These results indicate that UI Lakes still needs to implement some lake water quality management strategies."

"Mikroplastik yang mencemari perairan tawar dapat mengganggu keberlangsungan hidup biota di dalamnya. Penelitian ini bertujuan untuk menganalisis bentuk, warna, dan kelimpahan mikroplastik yang terkandung pada air, sedimen, insang, dan saluran pencernaan ikan red devil Amphilophus labiatus di Situ Kenanga dan Situ Mahoni, Universitas Indonesia, Depok, Jawa Barat. Sampel air dan sedimen di ambil pada 3 stasiun pengambilan sampel dari kedua situ. Sampel ikan red devil diperoleh 15 ekor pada masing – masing situ. Sampel insang dan saluran pencernaan dihancurkan menggunakan larutan asam nitrat (HNO3) 65%. Sampel air diambil 20 L dan disaring menggunakan plankton net. Sampel sedimen dikeringkan dalam oven sebanyak 25 gram. Larutan NaCl jenuh digunakan untuk memisahkan mikroplastik dengan pengotor. Sebanyak 1 mL sample diteteskan pada Sedgwick Rafter Chamber kemudian diamati dan dihitung berdasarkan bentuk mikroplastik di bawah mikroskop. Hasil penelitian menunjukkan kelimpahan mikroplastik bentuk fiber, fragmen, film, granula terdapat pada seluruh sampel dengan warna yang beragam. Hasil penelitian menunjukkan total kelimpahan rata – rata mikroplastik di Situ Kenanga sebanyak 434,33 ± 23,51 partikel L-1 pada air, 45.837,04 ± 36.305,97 partikel kg-1 pada sedimen, 268,33 ± 119,18 partikel gr-1 dan 1266,2 ± 349,72 partikel ind-1 pada insang, 287,79 ± 185,22 partikel gr-1 dan 978,22 ± 336,38 partikel ind-1 pada saluran pencernaan. Pada Situ Mahoni sebesar 437,67 ± 30,21 partikel L-1 pada air, 36.237,04 ± 16.702,59 partikel kg-1 pada sedimen, 429,18 ± 187,50 partikel gr-1 dan 1233,8 ± 253,60 partikel ind-1 pada insang, 318,04 ± 114,94 partikel gr-1 dan 1053,78 ± 328,44 partikel ind-1 pada saluran pencernaan.

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Microplastics that contaminate freshwater can disrupt the survival of the biota in it. This study aims to analyze the shape, color, and abundance of microplastics contained in water, sediment, gills, and digestive tract of red devil fish Amphilophus labiatus in Situ Kenanga and Situ Mahoni, University of Indonesia, Depok, West Java. Water and sediment samples were taken at 3 sampling stations from the two Situ. Samples of red devil fish were obtained 15 tails in each Situ. Samples of gills and digestive tract were destroyed using 65% nitric acid (HNO3) solution. Water samples were taken 20 L and filtered using a plankton net. Sediment samples were dried in an oven as much as 25 grams. Saturated NaCl solution was used to separate microplastics with impurities. A total of 1 mL of the sample was dropped into the Sedgwick Rafter Chamber and then observed and calculated based on the shape of the microplastic under a microscope. The results showed an abundance of microplastics in the form of fibers, fragments, films, and granules in all samples with various colors. The results showed the total average abundance of microplastics in Situ Kenanga was 434,33 ± 23,51 L-1 particles in water, 45.837,04 ± 36.305,97 particles kg-1 in sediments, 268.33 ± 119.18 particles gr-1 and 1266.2 ± 349.72 particles ind-1 in the gills, 287.79 ± 185.22 particles gr-1 and 978.22 ± 336.38 particles ind-1 in the digestive tract. At Situ Mahoni there are 437,67 ± 30,21 particles L-1 in water, 36.237,04 ± 16.702,59 particles kg-1 in sediments, 429.18 ± 187.50 particles gr-1 and 1233.8 ± 253.60 particles ind-1 on the gills, 318.04 ± 114.94 particles gr-1 and 1053.78 ± 328.44 particles ind-1 in the digestive tract.

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"Mikroplastik didefinisikan sebagai partikel plastik dengan ukuran <5 mm. Mikroplastik dapat masuk ke dalam tubuh organisme air khususnya ikan melalui oral dan insang. Penelitian ini bertujuan untuk mengetahui bentuk dan kelimpahan mikroplastik pada air, insang dan saluran pencernaan ikan mujair Oreochromis mossambicus (Peters, 1852) serta menguji perbedaan kelimpahan mikroplastik pada ikan mujair di Danau Kenanga dan Danau Agathis Universitas Indonesia. Penelitian dilakukan di Departemen Biologi FMIPA UI, Depok. Sampel ikan mujair diperoleh sebanyak total 20 ekor dari kedua danau dengan panjang tubuh relatif antara 16--19 cm. Insang dan saluran pencernaan dari 20 sampel ikan mujair diisolasi, kemudian diekstraksi dan dihancurkan menggunakan larutan asam nitrat (HNO3) 65%. Sampel yang telah diekstraksi kemudian diberi larutan NaCl jenuh agar mikroplastik mengapung ke permukaan. Sampel diteteskan sebanyak 1 ml pada Sedgwick Rafter Chamber kemudian diamati di bawah mikroskop dan dihitung jumlah mikroplastik berdasarkan bentuk partikelnya. Total kelimpahan rata-rata mikroplastik pada air Danau Kenanga sebesar 1.766,6 ± 40,11 partikel/L, sementara total kelimpahan rata-rata mikroplastik air pada Danau Agathis sebesar 1.885,53 ± 106,27 partikel/L. Total kelimpahan rata-rata mikroplastik ikan mujair pada Danau Kenanga di insang sebanyak 6.232 ± 1.898,66 partikel/ind dan di saluran pencernaan sebanyak 9.108 ± 4.027,14 partikel/ind, sementara total kelimpahan rata-rata mikroplastik ikan mujair pada Danau Agathis di insang sebanyak 6.716 ± 2.467,67 partikel/ind dan di saluran pencernaan sebanyak 4.038 ± 2.180,75 partikel/ind. Persentase komposisi bentuk mikroplastik yang ditemukan dominan pada Danau Kenanga terdapat bentuk fragmen sebesar 40% di air; fiber 80% di insang dan fiber 75% di saluran pencernaan, Sementara komposisi bentuk mikroplastik yang ditemukan dominan pada Danau Agathis terdapat bentuk fiber sebesar 43% di air; 75% di insang dan 67% di saluran pencernaan. Hasil analisis statistik Uji Mann-Whittney menunjukkan adanya perbedaan secara signifikan kelimpahan mikroplastik pada ikan mujair di Danau Kenanga dan Danau Agathis, Universitas Indonesia.

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Microplastics are define as plastic particles with a size of <5 mm. Microplastics can enter the body of aquatic organisms, especially fish through the mouth and gills. This study aims to determine the shape and abundance of microplastics in water, gill and digestive tract tilapia fish Oreochromis mossambicus (Peters, 1852) at Kenanga Pond and Agathis Pond, University of Indonesia and to analyze and examine differences in the abundance of microplastics in tilapia fish at Kenanga Pond and Agathis Pond, University of Indonesia. The study was conducted at Department of Biology FMIPA UI, Depok. Total 20 tilapia fish from the two lakes with relative length between 16--19 cm. Gills and digestive tract samples were obtained of tilapia fish were isolated, extracted and then crushed using a saturated solution of nitric acid (HNO3) 65%. The extracted sample is then given NaCl solution so that the microplastic floats to the surface. As much as 1 ml NaCl were dropped in the Sedgwick Rafter Chamber then observed under a microscope. The number of microplastic was calculated based on the type. The average total abundance of microplastics in the water of Kenanga Pond was 1.766,6 ± 40,11 particles/L, meanwhile the average total abundance of microplastics in the water of Agathis Pond was 1.885,53 ± 106,27 particles/L. The average total microplastics in tilapia gill at Kenanga Pond was 6.232 ± 1.898,66 particles/ind and in the digestive tract was 9.108 ± 4,027.14 particles/ind, meanwhile the average total abundance microplastics in tilapia gill at Agathis Pond was 6.716 ± 2.467,67 particles/ind and in the digestive tract was 4.038 ± 2.180,75 particles/ind. The percentage composition of the microplastic form that was found dominant at Kenanga Pond were as much as 40% fragments in the water; 80% fiber in the gills and 75% fiber in the digestive tract. Meanwhile, the percentage composition of the microplastic form that was found dominant at Agathis Pond were as much as 43% fiber in the water; 75% in the gills and 67% in the digestive tract. The results of the Mann-Whittney test statistical analysis showed that there was significant difference between abundance of microplastics in tilapia fish at of Kenanga Pond and Agathis Pond, University of Indonesia."

"Pencemaran mikroplastik menjadi ancaman bagi kehidupan biota. Mikroplastik yang terakumulasi dalam saluran pencernaan biota dapat menghambat pertumbuhan, mempengaruhi reproduksi, dan kerusakan organ internal. Penelitian ini bertujuan untuk mengetahui kelimpahan dan  bentuk mikroplastik pada air, sedimen, insang, dan saluran pencernaan Ikan Nila Oreochromis niloticus di Situ Rawa Besar, Depok. Sampel air dan sedimen di ambil pada 3 stasiun pengambilan sampel dari situ. Sampel Ikan Nila diambil 5 ekor masing-masing pada 3 stasiun. Sampel insang dan saluran pencernaan dihancurkan menggunakan larutan asam nitrat (HNO3) 65%. Sampel air diambil 20 L dan disaring menggunakan plankton net. Sampel sedimen dikeringkan dalam oven sebanyak 25 gram. Larutan NaCl jenuh digunakan untuk memisahkan mikroplastik dengan pengotor. Sebanyak 1 mL sampel diteteskan pada Sedgewick Rafter Chamber kemudian diamati dan dihitung berdasarkan bentuk mikroplastik di bawah mikroskop. Hasil penelitian pada Juli 2022 menunjukkan bahwa kelimpahan mikroplastik berkisar antara 15,00-23,33 partikel L-1 pada air, 20.330,00-45.330,00 partikel kg-1 pada sedimen, 182,67-593,33 partikel ind-1 pada insang, dan 596,00-701,33 partikel ind-1 pada saluran pencernaan. Kelimpahan mikroplastik tersebut lebih rendah dari kelimpahannya pada September 2023, yaitu berkisar antara 24,33-34,67 partikel L-1 pada air, 31.000,00-65.670,00 partikel kg-1 pada sedimen, 317,33-744,00 partikel ind-1 pada insang, dan 844,00-1.120,00 partikel ind-1 pada saluran pencernaan. Bentuk mikroplastik yang paling dominan adalah fragmen pada air, Ikan Nila, dan sedimen. Penelitian ini menunjukkan peningkatan jumlah mikroplastik di Situ Rawa Besar seiring berjalannya waktu yang didukung oleh beberapa faktor seperti peningkatan populasi, peningkatan sampah plastik, aktivitas antropogenik, dan curah hujan.

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Microplastic pollution poses a threat to the life of aquatic organisms. Accumulated microplastics in the digestive tracts of these organisms can hinder growth, affect reproduction, and cause internal organ damage. This research aims to determine the abundance and forms of microplastics in water, sediment, gills, and digestive tracts of Nile Tilapia (Oreochromis niloticus) in Situ Rawa Besar, Depok. Water and sediment samples were collected from three sampling stations in the lake. Five Nile Tilapia samples were taken from each station. Gill and digestive tract samples were digested using 65% nitric acid (HNO3) solution. Twenty liters of water were collected and filtered using a plankton net. Sediment samples were dried in an oven, with 25 grams used for analysis. A saturated NaCl solution was employed to separate microplastics from impurities. One milliliter of the sample was dropped onto a Sedgewick Rafter Chamber, then observed and counted based on microplastic forms under a microscope. The research results in July 2022 indicated that microplastic abundance ranged from 15.00 to 23.33 particles L-1 in water, 20,330.00 to 45,330.00 particles kg-1 in sediment, 182.67 to 593.33 particles ind-1 in gills, and 596.00 to 701.33 particles ind-1 in the digestive tract. These abundances were lower than those in September 2023, ranging from 24.33 to 34.67 particles L-1 in water, 31,000.00 to 65,670.00 particles kg-1 in sediment, 317.33 to 744.00 particles ind-1 in gills, and 844.00 to 1,120.00 particles ind-1 in the digestive tract. The most dominant form of microplastics was fragments in water, Nile Tilapia, and sediment. This research indicates an increase in the quantity of microplastics in Situ Rawa Besar over time, supported by factors such as population growth, increased plastic waste, anthropogenic activities, and rainfall."

"Telah dilakukan penelitian yang bertujuan untuk menganalisis bentuk dan kelimpahan mikroplastik pada air, insang, dan saluran pencernaan ikan mujair Oreochromis mossambicus dan ikan setan merah Amphilophus labiatus yang terdapat di Situ Salam, Kampus Universitas Indonesia, Depok. Sampel air diambil sebanyak 20 L lalu disaring menggunakan plankton net sampai volume air menjadi 200 ml sementara sampel ikan mujair dan ikan setan merah diambil masing-masing 10 ekor dengan jala lalu disimpan di wadah penyimpanan berisi formalin 40%. Ekstraksi sampel dilakukan di Laboratorium Biologi Kelautan, Departemen Biologi, FMIPA UI dengan metode dekstruksi oleh HNO3 65% kemudian dilakukan analisis bentuk dan kelimpahan mikroplastik di bawah mikroskop. Hasil penelitian menunjukkan, rata-rata total kelimpahan mikroplastik pada air diperoleh 153,7 ± 44,2 partikel L-1, pada saluran pencernaan ikan mujair diperoleh 2.868 ± 723,5 partikel ind-1, pada saluran pencernaan ikan setan merah diperoleh 3.548,4 ± 1031,4 partikel ind-1, pada insang ikan mujair diperoleh 3.782,6 ± 1.171,6 partikel ind-1, dan pada insang ikan setan merah diperoleh 3.848 ± 863,1 partikel ind-1. Bentuk mikroplastik yang mendominasi pada air situ, saluran pencernaan, dan insang adalah bentuk fiber. Berdasarkan hasil Uji T Dua Sampel terhadap sampel insang dan pencernaan dari ikan mujair dan ikan setan merah menunjukkan tidak ada perbedaan signifikan.

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Research has been carried out that aims to analyze the shape and microplastics in the water, gills, and digestive tract of tilapia fish Oreochromis mossambicus and red devil fish Amphilophus labiatus that found in Salam Lake, University of Indonesia Campus, Depok. Water samples were taken as much as 20 L and then filtered using a plankton net until the water volume became 200 ml while samples of tilapia fish and red devil fish were taken each with a net and stored in a storage container containing formalin 40%. Sample extraction was carried out at the Marine Biology Laboratory, Department of Biology, FMIPA UI with the destruction method by HNO3 65% and then analyzed the shape and abundance of microplastics under a microscope. The results showed that the average total abundance of microplastics in water was 153,7 ± 44,2 L-1 particles, in the digestive tract of tilapia fish it was obtained 2.868 ± 723,5 ind-1 particles, in the digestive tract of red devil fish obtained 3.548,4 ± 1.031,4 ind-1 particles, in the gills of tilapia fish obtained 3.782,6 ± 1.171,6 ind-1 particles, and in the gills of red devil fish obtained 3.848 ± 863,1 particles ind-1. The predominant form of microplastic in the water, digestive tract, and gills is the form of fiber. Based on the results of the Two-Sample T-Test on gill and digestive samples of tilapia fish and red devil fish, there is no significant difference."