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"Telah dilakukan penelitian yang bertujuan untuk menganalisis bentuk dan kelimpahan mikroplastik pada air, insang, dan saluran pencernaan ikan mujair Oreochromis mossambicus dan ikan setan merah Amphilophus labiatus yang terdapat di Situ Salam, Kampus Universitas Indonesia, Depok. Sampel air diambil sebanyak 20 L lalu disaring menggunakan plankton net sampai volume air menjadi 200 ml sementara sampel ikan mujair dan ikan setan merah diambil masing-masing 10 ekor dengan jala lalu disimpan di wadah penyimpanan berisi formalin 40%. Ekstraksi sampel dilakukan di Laboratorium Biologi Kelautan, Departemen Biologi, FMIPA UI dengan metode dekstruksi oleh HNO3 65% kemudian dilakukan analisis bentuk dan kelimpahan mikroplastik di bawah mikroskop. Hasil penelitian menunjukkan, rata-rata total kelimpahan mikroplastik pada air diperoleh 153,7 ± 44,2 partikel L-1, pada saluran pencernaan ikan mujair diperoleh 2.868 ± 723,5 partikel ind-1, pada saluran pencernaan ikan setan merah diperoleh 3.548,4 ± 1031,4 partikel ind-1, pada insang ikan mujair diperoleh 3.782,6 ± 1.171,6 partikel ind-1, dan pada insang ikan setan merah diperoleh 3.848 ± 863,1 partikel ind-1. Bentuk mikroplastik yang mendominasi pada air situ, saluran pencernaan, dan insang adalah bentuk fiber. Berdasarkan hasil Uji T Dua Sampel terhadap sampel insang dan pencernaan dari ikan mujair dan ikan setan merah menunjukkan tidak ada perbedaan signifikan.

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Research has been carried out that aims to analyze the shape and microplastics in the water, gills, and digestive tract of tilapia fish Oreochromis mossambicus and red devil fish Amphilophus labiatus that found in Salam Lake, University of Indonesia Campus, Depok. Water samples were taken as much as 20 L and then filtered using a plankton net until the water volume became 200 ml while samples of tilapia fish and red devil fish were taken each with a net and stored in a storage container containing formalin 40%. Sample extraction was carried out at the Marine Biology Laboratory, Department of Biology, FMIPA UI with the destruction method by HNO3 65% and then analyzed the shape and abundance of microplastics under a microscope. The results showed that the average total abundance of microplastics in water was 153,7 ± 44,2 L-1 particles, in the digestive tract of tilapia fish it was obtained 2.868 ± 723,5 ind-1 particles, in the digestive tract of red devil fish obtained 3.548,4 ± 1.031,4 ind-1 particles, in the gills of tilapia fish obtained 3.782,6 ± 1.171,6 ind-1 particles, and in the gills of red devil fish obtained 3.848 ± 863,1 particles ind-1. The predominant form of microplastic in the water, digestive tract, and gills is the form of fiber. Based on the results of the Two-Sample T-Test on gill and digestive samples of tilapia fish and red devil fish, there is no significant difference."

"Mikroplastik didefinisikan sebagai partikel plastik dengan ukuran <5 mm. Mikroplastik dapat masuk ke dalam tubuh organisme air khususnya ikan melalui oral dan insang. Penelitian ini bertujuan untuk mengetahui bentuk dan kelimpahan mikroplastik pada air, insang dan saluran pencernaan ikan mujair Oreochromis mossambicus (Peters, 1852) serta menguji perbedaan kelimpahan mikroplastik pada ikan mujair di Danau Kenanga dan Danau Agathis Universitas Indonesia. Penelitian dilakukan di Departemen Biologi FMIPA UI, Depok. Sampel ikan mujair diperoleh sebanyak total 20 ekor dari kedua danau dengan panjang tubuh relatif antara 16--19 cm. Insang dan saluran pencernaan dari 20 sampel ikan mujair diisolasi, kemudian diekstraksi dan dihancurkan menggunakan larutan asam nitrat (HNO3) 65%. Sampel yang telah diekstraksi kemudian diberi larutan NaCl jenuh agar mikroplastik mengapung ke permukaan. Sampel diteteskan sebanyak 1 ml pada Sedgwick Rafter Chamber kemudian diamati di bawah mikroskop dan dihitung jumlah mikroplastik berdasarkan bentuk partikelnya. Total kelimpahan rata-rata mikroplastik pada air Danau Kenanga sebesar 1.766,6 ± 40,11 partikel/L, sementara total kelimpahan rata-rata mikroplastik air pada Danau Agathis sebesar 1.885,53 ± 106,27 partikel/L. Total kelimpahan rata-rata mikroplastik ikan mujair pada Danau Kenanga di insang sebanyak 6.232 ± 1.898,66 partikel/ind dan di saluran pencernaan sebanyak 9.108 ± 4.027,14 partikel/ind, sementara total kelimpahan rata-rata mikroplastik ikan mujair pada Danau Agathis di insang sebanyak 6.716 ± 2.467,67 partikel/ind dan di saluran pencernaan sebanyak 4.038 ± 2.180,75 partikel/ind. Persentase komposisi bentuk mikroplastik yang ditemukan dominan pada Danau Kenanga terdapat bentuk fragmen sebesar 40% di air; fiber 80% di insang dan fiber 75% di saluran pencernaan, Sementara komposisi bentuk mikroplastik yang ditemukan dominan pada Danau Agathis terdapat bentuk fiber sebesar 43% di air; 75% di insang dan 67% di saluran pencernaan. Hasil analisis statistik Uji Mann-Whittney menunjukkan adanya perbedaan secara signifikan kelimpahan mikroplastik pada ikan mujair di Danau Kenanga dan Danau Agathis, Universitas Indonesia.

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Microplastics are define as plastic particles with a size of <5 mm. Microplastics can enter the body of aquatic organisms, especially fish through the mouth and gills. This study aims to determine the shape and abundance of microplastics in water, gill and digestive tract tilapia fish Oreochromis mossambicus (Peters, 1852) at Kenanga Pond and Agathis Pond, University of Indonesia and to analyze and examine differences in the abundance of microplastics in tilapia fish at Kenanga Pond and Agathis Pond, University of Indonesia. The study was conducted at Department of Biology FMIPA UI, Depok. Total 20 tilapia fish from the two lakes with relative length between 16--19 cm. Gills and digestive tract samples were obtained of tilapia fish were isolated, extracted and then crushed using a saturated solution of nitric acid (HNO3) 65%. The extracted sample is then given NaCl solution so that the microplastic floats to the surface. As much as 1 ml NaCl were dropped in the Sedgwick Rafter Chamber then observed under a microscope. The number of microplastic was calculated based on the type. The average total abundance of microplastics in the water of Kenanga Pond was 1.766,6 ± 40,11 particles/L, meanwhile the average total abundance of microplastics in the water of Agathis Pond was 1.885,53 ± 106,27 particles/L. The average total microplastics in tilapia gill at Kenanga Pond was 6.232 ± 1.898,66 particles/ind and in the digestive tract was 9.108 ± 4,027.14 particles/ind, meanwhile the average total abundance microplastics in tilapia gill at Agathis Pond was 6.716 ± 2.467,67 particles/ind and in the digestive tract was 4.038 ± 2.180,75 particles/ind. The percentage composition of the microplastic form that was found dominant at Kenanga Pond were as much as 40% fragments in the water; 80% fiber in the gills and 75% fiber in the digestive tract. Meanwhile, the percentage composition of the microplastic form that was found dominant at Agathis Pond were as much as 43% fiber in the water; 75% in the gills and 67% in the digestive tract. The results of the Mann-Whittney test statistical analysis showed that there was significant difference between abundance of microplastics in tilapia fish at of Kenanga Pond and Agathis Pond, University of Indonesia."

"Curah hujan di musim hujan berperan dalam mentransportasikan sampah, termasuk mikroplastik dari sungai ke laut. Peningkatan sampah, arus, dan peningkatan penduduk juga dapat memengaruhi kelimpahan mikroplastik. Mikroplastik di pesisir dapat menempel pada daun lamun. Daun lamun akan mengalami pertumbuhan, sehingga memungkinkan adanya perbedaan kelimpahan mikroplastik di sepanjang daun lamun. Penelitian ini bertujuan untuk menganalisis perbandingan kelimpahan mikroplastik pada daun Enhalus acoroides, air, dan sedimen di Pulau Lima, Teluk Banten pada musim hujan tahun 2020 dan 2021, serta menganalisis apakah ada perbedaan kelimpahan mikroplastik di sepanjang daun E. acoroides. Sampel daun diambil seluas 1 cm2 dari helaian daun, kemudian dikerik menggunakan cutter. Sampel air sebanyak 20 L diambil menggunakan plankton net. Sampel sedimen sebanyak 200 g dikeringkan menggunakan oven. Rata-rata kelimpahan mikroplastik pada sampel daun lamun tahun 2020 sebesar 46,96±3,13 partikel/cm2, dan tahun 2021 sebesar 61,5±6,63 partikel/cm2. Sampel air tahun 2020 sebesar 130,66±14,19 partikel/L, sedangkan 2021 sebesar 162,22±7,82 partikel/L. Sampel sedimen tahun 2020 sebesar 12.066±4.017,6 partikel/Kg, sedangkan 2021 sebesar 17.354,67±2.341,95 partikel/Kg. Terdapat peningkatan kelimpahan mikroplastik pada semua sampel di Pulau Lima, Teluk Banten saat musim hujan tahun 2020 hingga 2021. Rata-rata kelimpahan mikroplastik pada jarak 20 cm dari pangkal daun sebanyak 36,7±7,8 partikel/cm2 lebih rendah daripada jarak 50 cm sebanyak 144,4±23,74 partikel/cm2. Terdapat perbedaan kelimpahan mikroplastik di sepanjang daun lamun.

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Rainfall in the rainy season plays a role in transporting waste, including microplastics from rivers to the sea. Increased waste, currents, and population growth can also affect the abundance of microplastics. Microplastics on the coast are attached to seagrass leaves. Seagrass leaves experienced growth, thus allowing for differences in the abundance of microplastics along with the leaves. This study aims to analyze the comparison of the abundance of microplastics in Enhalus acoroides leaves, water, and sediment on Lima Island, Banten Bay in the rainy seasons of 2020 and 2021, and analyze whether there are differences in the abundance of microplastics along with E. acoroides leaves. The leaves were taken 1 cm2 from the leaf blade, then scraped using a cutter. The water (20 L) was taken using a plankton net. The sediment (200 g) was dried using an oven. The average abundance of microplastics in seagrass leaf samples in 2020 was 46.96±3.13 particles/cm2, while in 2021 was 61.5±6.63 particles/cm2. The water sample in 2020 was 130.66±14.19 particles/L, while in 2021 was 162.22±7.82 particles/L. The sediment sample in 2020 was 12,066±4,017.6 particles/Kg, while in 2021 was 17,354.67±2,341.95 particles/Kg. There was an increase in the abundance of microplastics in all samples on Lima Island, Banten Bay during the rainy season from 2020 to 2021. The average abundance of microplastics at a distance of 20 cm from the base of the leaf was 36.7±7.8 particles/cm2, lower than a distance of 50 cm at 144.4±23.74 particles/cm2. There were differences in the abundance of microplastics along with the seagrass leaves."

"Laut merupakan sumber daya dengan peran penting dalam mempertahankan produksi pangan. Namun, diperkirakan 51 triliun partikel mikroplastik tersebar di seluruh laut dunia. Polusi mikroplastik memungkinkan paparan ke manusia melalui jaring-jaring makanan karena dapat menempel pada biota laut yang edible seperti makroalga anggur laut. Penelitian pada anggur laut (Caulerpa racemosa) di Pulau Semak Daun bertujuan untuk mengetahui bentuk dan total kelimpahan mikroplastik melalui kontrol positif 10 gram sampel dalam 100 ml akuades, metode pengadukkan dengan magnetic strirrer dan metode penghancuran menggunakan NaOH. Hasil penelitian menemukan mikroplastik fiber, fragmen, film, dan foam sebanyak 10,34 partikel/gram. Penelitian ini memberi bukti bahwa mikroplastik dapat menempel ke anggur laut dan unsur abiotik di sekitarnya sehingga beresiko masuk ke rantai makanan karena dikonsumsi oleh manusia secara langsung maupun tidak langsung melalui biota laut lain.

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The ocean is a resource with an important role in maintaining global food production. However, an estimated 51 trillion microplastic particles are scattered throughout the world's oceans. Microplastic pollution allows exposure to humans through food webs because it can attach to edible marine biota such as sea grape macroalgae. Research on sea grapes (Caulerpa racemosa) in Pulau Semak Daun with objectives to determine the shape and total abundance of microplastics through positive control of 10 grams of sample in 100 ml of distilled water, stirring method with magnetic stirrer and destruction method using NaOH. The results of this research found microplastic fibers, fragments, films, and foams with the total abundance is 10.34 particles/gram. This research provides evidence that microplastics can stick to sea grapes and the surrounding abiotic elements and risk of entering the food chain because they are consumed by humans directly or indirectly through other marine biota."

"Makroalga merupakan tumbuhan yang banyak dimanfaatkan sebagai sumber makanan bagi organisme laut dan manusia. Makroalga Sargassum cinereum pada bagian permukaan thalli dapat terakumulasi oleh miktoplastik yang memiliki daya akumulasi tinggi di perairan Pulau Semak Daun. Kontaminasi mikroplastik pada bagian makroalga terjadi karena adanya akumulasi mikroplastik pada tempat tumbuh makroalga. Mikroplastik dapat menempel, melilit, maupun terbungkus oleh makroalga. Penelitian ini bertujuan untuk mengetahui bentuk, warna, dan ukuran mikroplastik dan pengaruh variasi waktu pengocokkan terhadap pengurangan kelimpahan mikroplastik pada makroalga Sargassum cinereum J. Agardh 1848. Sampel pada permukaan makroalga diambil sebanyak 10 g, kemudian diberi perlakuan pengocokkan dengan perbedaan waktu 5 menit, 10 menit, dan 15 menit dan disaring menggunakan kertas saring Whatman filter. Sampel yang menempel kuat pada makroalga dilarutkan dengan larutan NaOH, kemudian disaring menggunkan kertas Whatman filter. Sampel kontrol negatif di diamkan selama 15 menit, sedangkan kontrol positif langsung dilakukan penyaringan. Bentuk mikroplastik didominasi oleh fiber sebesar 58% dengan jumlah 36,5 partikel/g. Warna mikroplastik didominasi oleh biru sebesar 75%. Persentase pengurangan kelimpahan mikroplastik pada kelompok perlakuan tertinggi terdapat pada perlakuan pengocokkan selama 15 menit (K15) sebesar 70% dan terendah pada perlakuan kontrol negatif (KN) sebesar 45%. Uji One Way ANOVA membuktikan bahwa adanya perbedaan rata-rata kelimpahan mikroplastik pada kelompok perlakuan pengocokkan dengan nilai signifikan sebesar 0,002 (< 0,05). Waktu pengocokkan mempengaruhi pengurangan kelimpahan mikroplastik pada makroalga Sargassum cinereum.

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Macroalgae are plants that are widely used as a food source for marine organisms and humans. Sargassum cinereum macroalgae on the surface of the thalli can be accumulated by mytoplastics which have high accumulation power in the waters of Semak Daun Island. Microplastic contamination in the macroalgae section occurs due to the accumulation of microplastics in the macroalgae growing sites. Microplastics can be attached, wrapped around, or covered by macroalgae. This study aims to determine the shape, color, and size of microplastics and the effect of variations in shaking time on the reduction of microplastics in macroalgae Sargassum cinereum J. Agardh 1848. Samples on the surface of macroalgae were taken as much as 10 g, then given shaking treatment with a time difference of 5 minutes, 10 minutes, and 15 minutes and filtered using a Whatman filter paper filter. Samples that adhered strongly to macroalgae were dissolved with NaOH solution, then filtered using Whatman filter paper. The negative control sample was allowed to stand for 15 minutes, while the positive control sample was immediately assessed. The form of microplastic is dominated by fiber by 58% with a total of 36.5 particles/g. The color of microplastic is dominated by blue by 75%. The percentage of microplastic reduction in the treatment group was highest in the shaking treatment for 15 minutes (K15) by 70% and the lowest in the negative treatment (KN) 45%. The One-Way ANOVA test proved that the difference between the mean and microplastics in the shaking treatment group had a significant value of 0.002 (< 0.05). Shaking time affects the reduction of microplastic in macroalga Sargassum cinereum."

"Ikan Nila Oreochromis niloticus (Linnaeus, 1758) pada Setu Babakan Jagakarsa, Jakarta Selatan banyak dikonsumsi oleh masyarakat sekitar. Penelitian ini bertujuan untuk menganalisis presentase kelimpahan bentuk mikroplastik pada insang, lambung, dan usus ikan Nila Oreochromis niloticus (Linnaeus, 1758) yang diperoleh dari Setu Babakan, Jagakarsa, Jakarta Selatan. Penelitian dilakukan di Setu Babakan dan Laboratorium Biologi Laut, Departemen Biologi FMIPA Universitas Indonesia. Sampel ikan Nila yang diteliti sebanyak 18 ekor dengan panjang berkisar 13-20 cm. Sampel diisolasi untuk kemudian dilakukan metode destruksi dengan menggunakan larutan HNO3 65% sehingga dapat dilakukan proses analisis kelimpahan dan bentuk mikroplastik di bawah mikroskop. Hasil penelitian menunjukkan terdapat total kelimpahan rata-rata pada sampel air permukaan sebesar 308,00 ± 128,29 partikel/liter; insang sebesar 7.820 ± 1.847 partikel/individu; pada sampel lambung sebesar 2.588,11 ± 751,33 partikel/individu; dan pada sampel usus sebesar 5.178,16 ± 2.098,55 partikel/individu. Total kelimpahan mikroplastik terbesar terdapat pada sampel insang dan terendah pada sampel lambung. Hasil uji statistik Kruskal-Wallis menunjukkan tidak ada perbedaan presentase bentuk mikroplastik (fiber, film, fragmen dan granula) yang signifikan pada sampel insang, lambung dan usus ikan Nila Oreochromis niloticus (Linnaeus,1758) di Setu Babakan Jagakarsa, Jakarta Selatan

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Nile Tilapia Oreochromis niloticus (Linnaeus, 1758) in Setu Babakan Jagakarsa, South Jakarta is widely consumed by the local communities. This study analyzed percentage abudance of microplastics form in the gills, stomach, and intestinal of Nile Tilapia Oreochromis niloticus (Linnaeus, 1758) from Setu Babakan Jagakarsa, South Jakarta. This study was conducted at Setu Babakan and Marine Biology Laboratory, Departement of Biology FMIPA UI, Depok. The samples of Nile Tilapia studied were 18 individuals with the same relative length between 13-20 cm. The sample were isolated in order to do the destruction methods with HNO3 65%, then analysis of abundance and shape of microplastics can be done. The results show that the average total abudance in the gills sample was 7.820 ± 1.847 particles/individual; in the stomach was 2.588,11 ± 751,33 particles/individual; and in the intenstine was 5.178,16 ± 2.098,55 particles/individuals. The highest total abudance of microplastics was found in the gills and the lowest in the stomach samples. The results from Kruskal-Wallis statistical test shows there was no significant difference in the percentage of microplastics form (fiber, film, fragment, and granules) in the gills, stomach, and intestinal samples of Nile Tilapia Oreochromis niloticus (Linnaeus,1758) in Setu Babakan, Jagakarsa, South Jakarta."

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Mikroplastik merupakan partikel plastik yang terdegradasi di lingkungan dengan ukuran < 5 mm. Kandungan mikroplastik di perairan tawar berpotensi membahayakan kelangsungan hidup biota di dalamnya. Penelitian ini bertujuan menganalisis kelimpahan dan persentase komposisi bentuk mikroplastik pada insang dan saluran pencernaan ikan nila Oreochromis niloticus (Linnaeus,1758) di Ciliwung daerah Srengseng Sawah, Jakarta Selatan. Insang dan saluran pencernaan (lambung dan usus) dari 10 sampel ikan nila diekstraksi dan dihancurkan menggunakan larutan asam nitrat (HNO₃) 65%. Sampel yang telah diekstraksi kemudian diberi larutan NaCl jenuh agar mikroplastik mengapung ke permukaan. Sampel diteteskan sebanyak 1 ml pada Sedgwick Rafter Chamber kemudian diamati di bawah mikroskop dan dihitung jumlah mikroplastik berdasarkan bentuk partikelnya. Hasil penelitian terdapat kelimpahan mikroplastik pada sampel insang, lambung, dan usus ikan nila diantaranya bentuk fiber, film, fragmen, dan granula. Total kelimpahan rata-rata mikroplastik di insang sebanyak 4.135 ± 3.297,20 partikel/ind, di lambung sebanyak 2.772 ± 1.030,43 partikel/ind, dan di usus sebanyak 3.598 ± 1.582,18 partikel/ind ditemukan pada ikan nila. Persentase komposisi bentuk mikroplastik yang ditemukan dominan pada ikan nila terdapat bentuk fiber sebesar 57,85% di insang; 53,21% di lambung dan 53,06% di usus.

 

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Microplastics are plastic particles that degrade in the environment with a size of <5 mm. The content of microplastics in freshwater has the potential to endanger the survival of the biota in it. This study aims to analyze the abundance and percentage composition of microplastic forms in the gills and digestive tract of Nile Tilapia fish Oreochromis niloticus (Linnaeus, 1758) in Ciliwung, Srengseng Sawah, South Jakarta. The gills and digestive tract (stomach and intestines) of 10 samples of nile tilapia fish were extracted and crushed using a 65% nitric acid (HNO3) solution. The extracted sample is then given a saturated NaCl solution so that the microplastic floats to the surface. The sample was dropped as much as 1 ml into the Sedgwick Rafter Chamber and then observed under a microscope and the number of microplastics was calculated based on the shape of the particles. The results showed an abundance of microplastics in the samples of gill, stomach, and intestines of tilapia including the form of fiber, film, fragments, and granules. The total abundance of microplastics in the gills was 4.135 ± 3.297,20 particles / ind, 2.772 ± 1.030,43 particles / ind in the stomach, and 3.598 ± 1.582,18 particles / ind in the intestine, which was found in tilapia. The percentage composition of the microplastic form that was found predominantly in tilapia contained a fiber form of 57,85% in the gills; 53,21% in the stomach and 53,06% in the intestine.

 

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"Pencemaran mikroplastik menjadi ancaman bagi kehidupan biota. Mikroplastik yang terakumulasi dalam saluran pencernaan biota dapat menghambat pertumbuhan, mempengaruhi reproduksi, dan kerusakan organ internal. Penelitian ini bertujuan untuk mengetahui kelimpahan dan  bentuk mikroplastik pada air, sedimen, insang, dan saluran pencernaan Ikan Nila Oreochromis niloticus di Situ Rawa Besar, Depok. Sampel air dan sedimen di ambil pada 3 stasiun pengambilan sampel dari situ. Sampel Ikan Nila diambil 5 ekor masing-masing pada 3 stasiun. Sampel insang dan saluran pencernaan dihancurkan menggunakan larutan asam nitrat (HNO3) 65%. Sampel air diambil 20 L dan disaring menggunakan plankton net. Sampel sedimen dikeringkan dalam oven sebanyak 25 gram. Larutan NaCl jenuh digunakan untuk memisahkan mikroplastik dengan pengotor. Sebanyak 1 mL sampel diteteskan pada Sedgewick Rafter Chamber kemudian diamati dan dihitung berdasarkan bentuk mikroplastik di bawah mikroskop. Hasil penelitian pada Juli 2022 menunjukkan bahwa kelimpahan mikroplastik berkisar antara 15,00-23,33 partikel L-1 pada air, 20.330,00-45.330,00 partikel kg-1 pada sedimen, 182,67-593,33 partikel ind-1 pada insang, dan 596,00-701,33 partikel ind-1 pada saluran pencernaan. Kelimpahan mikroplastik tersebut lebih rendah dari kelimpahannya pada September 2023, yaitu berkisar antara 24,33-34,67 partikel L-1 pada air, 31.000,00-65.670,00 partikel kg-1 pada sedimen, 317,33-744,00 partikel ind-1 pada insang, dan 844,00-1.120,00 partikel ind-1 pada saluran pencernaan. Bentuk mikroplastik yang paling dominan adalah fragmen pada air, Ikan Nila, dan sedimen. Penelitian ini menunjukkan peningkatan jumlah mikroplastik di Situ Rawa Besar seiring berjalannya waktu yang didukung oleh beberapa faktor seperti peningkatan populasi, peningkatan sampah plastik, aktivitas antropogenik, dan curah hujan.

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Microplastic pollution poses a threat to the life of aquatic organisms. Accumulated microplastics in the digestive tracts of these organisms can hinder growth, affect reproduction, and cause internal organ damage. This research aims to determine the abundance and forms of microplastics in water, sediment, gills, and digestive tracts of Nile Tilapia (Oreochromis niloticus) in Situ Rawa Besar, Depok. Water and sediment samples were collected from three sampling stations in the lake. Five Nile Tilapia samples were taken from each station. Gill and digestive tract samples were digested using 65% nitric acid (HNO3) solution. Twenty liters of water were collected and filtered using a plankton net. Sediment samples were dried in an oven, with 25 grams used for analysis. A saturated NaCl solution was employed to separate microplastics from impurities. One milliliter of the sample was dropped onto a Sedgewick Rafter Chamber, then observed and counted based on microplastic forms under a microscope. The research results in July 2022 indicated that microplastic abundance ranged from 15.00 to 23.33 particles L-1 in water, 20,330.00 to 45,330.00 particles kg-1 in sediment, 182.67 to 593.33 particles ind-1 in gills, and 596.00 to 701.33 particles ind-1 in the digestive tract. These abundances were lower than those in September 2023, ranging from 24.33 to 34.67 particles L-1 in water, 31,000.00 to 65,670.00 particles kg-1 in sediment, 317.33 to 744.00 particles ind-1 in gills, and 844.00 to 1,120.00 particles ind-1 in the digestive tract. The most dominant form of microplastics was fragments in water, Nile Tilapia, and sediment. This research indicates an increase in the quantity of microplastics in Situ Rawa Besar over time, supported by factors such as population growth, increased plastic waste, anthropogenic activities, and rainfall."

"Mikroplastik yang mencemari perairan tawar dapat mengganggu keberlangsungan hidup biota di dalamnya. Penelitian ini bertujuan untuk menganalisis bentuk, warna, dan kelimpahan mikroplastik yang terkandung pada air, sedimen, insang, dan saluran pencernaan ikan red devil Amphilophus labiatus di Situ Kenanga dan Situ Mahoni, Universitas Indonesia, Depok, Jawa Barat. Sampel air dan sedimen di ambil pada 3 stasiun pengambilan sampel dari kedua situ. Sampel ikan red devil diperoleh 15 ekor pada masing – masing situ. Sampel insang dan saluran pencernaan dihancurkan menggunakan larutan asam nitrat (HNO3) 65%. Sampel air diambil 20 L dan disaring menggunakan plankton net. Sampel sedimen dikeringkan dalam oven sebanyak 25 gram. Larutan NaCl jenuh digunakan untuk memisahkan mikroplastik dengan pengotor. Sebanyak 1 mL sample diteteskan pada Sedgwick Rafter Chamber kemudian diamati dan dihitung berdasarkan bentuk mikroplastik di bawah mikroskop. Hasil penelitian menunjukkan kelimpahan mikroplastik bentuk fiber, fragmen, film, granula terdapat pada seluruh sampel dengan warna yang beragam. Hasil penelitian menunjukkan total kelimpahan rata – rata mikroplastik di Situ Kenanga sebanyak 434,33 ± 23,51 partikel L-1 pada air, 45.837,04 ± 36.305,97 partikel kg-1 pada sedimen, 268,33 ± 119,18 partikel gr-1 dan 1266,2 ± 349,72 partikel ind-1 pada insang, 287,79 ± 185,22 partikel gr-1 dan 978,22 ± 336,38 partikel ind-1 pada saluran pencernaan. Pada Situ Mahoni sebesar 437,67 ± 30,21 partikel L-1 pada air, 36.237,04 ± 16.702,59 partikel kg-1 pada sedimen, 429,18 ± 187,50 partikel gr-1 dan 1233,8 ± 253,60 partikel ind-1 pada insang, 318,04 ± 114,94 partikel gr-1 dan 1053,78 ± 328,44 partikel ind-1 pada saluran pencernaan.

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Microplastics that contaminate freshwater can disrupt the survival of the biota in it. This study aims to analyze the shape, color, and abundance of microplastics contained in water, sediment, gills, and digestive tract of red devil fish Amphilophus labiatus in Situ Kenanga and Situ Mahoni, University of Indonesia, Depok, West Java. Water and sediment samples were taken at 3 sampling stations from the two Situ. Samples of red devil fish were obtained 15 tails in each Situ. Samples of gills and digestive tract were destroyed using 65% nitric acid (HNO3) solution. Water samples were taken 20 L and filtered using a plankton net. Sediment samples were dried in an oven as much as 25 grams. Saturated NaCl solution was used to separate microplastics with impurities. A total of 1 mL of the sample was dropped into the Sedgwick Rafter Chamber and then observed and calculated based on the shape of the microplastic under a microscope. The results showed an abundance of microplastics in the form of fibers, fragments, films, and granules in all samples with various colors. The results showed the total average abundance of microplastics in Situ Kenanga was 434,33 ± 23,51 L-1 particles in water, 45.837,04 ± 36.305,97 particles kg-1 in sediments, 268.33 ± 119.18 particles gr-1 and 1266.2 ± 349.72 particles ind-1 in the gills, 287.79 ± 185.22 particles gr-1 and 978.22 ± 336.38 particles ind-1 in the digestive tract. At Situ Mahoni there are 437,67 ± 30,21 particles L-1 in water, 36.237,04 ± 16.702,59 particles kg-1 in sediments, 429.18 ± 187.50 particles gr-1 and 1233.8 ± 253.60 particles ind-1 on the gills, 318.04 ± 114.94 particles gr-1 and 1053.78 ± 328.44 particles ind-1 in the digestive tract.

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"Analisis Kelimpahan Mikroplastik pada Insang dan Saluran Pencernaan Ikan Sapu-sapu Pterygoplichtys pardalis (Castelnau, 1855), Air, dan Sedimen di Ciliwung Daerah Tanjung Barat dan Daerah MT Haryono, Jakarta Selatan

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Abundance Analysis of Microplastic in Gills dan Digestive Tract of Sailfin Catfish Pterygoplichthys pardalis (Castelnau, 1855), Water, and Sediment in Tanjung Barat Area and MT Haryono Area, Ciliwung, South Jakarta"