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"Latar Belakang: Celah bibir dan palatum adalah keadaan dimana terdapat gangguan fusi atau celah abnormal bawaan pada daerah bibir atas, alveolar, dan palatum serta dapat menimbulkan masalah pada penderita seperti gangguan estetika dan masalah saat berbicara. Perawatan rekonstruksi tulang dengan autologous bone graft merupakan baku emas pada perawatan pasien celah bibir dan palatum, tetapi perawatan ini memiliki kekurangan sehingga dikembangkan alternatif perawatan seperti teknik rekayasa jaringan. Sumber sel stromal mesenkim yang digunakan dapat berasal dari jaringan pulpa gigi seperti sel stromal pulpa gigi sulung dan sel stromal pulpa gigi permanen. Kemampuan diferensiasi osteogenik sel stromal pulpa gigi sulung dan permanen pasien celah bibir dan palatum merupakan salah satu pertimbangan untuk penggunaan sel autologous dalam perawatan teknik rekayasa jaringan, sedangkan kemampuan diferensiasi osteogenik dari sel stromal pulpa gigi pasien CLP belum diketahui. Tujuan: Membandingkan kemampuan diferensiasi osteogenik sel stromal pulpa gigi sulung dan gigi permanen pasien celah bibir dan palatum melalui ekspresi gen ALP. Metode: Sampel yang diisolasi dari jaringan pulpa gigi sulung dan gigi permanen pasien celah bibir dan palatum dikultur pada medium osteogenik, dilakukan ekstraksi RNA dan diuji dengan Real-Time Polymerase Chain Reaction (RT PCR) menggunakan primers alkaline phosphatase (ALP) dan 18s housekeeping gene. Hasil: Ekspresi relatif gen ALP pada sel stromal pulpa gigi sulung pasien celah bibir dan palatum setelah dilakukan uji statistik tidak memiliki perbedaan bermakna bila dibandingkan dengan sel stromal pulpa gigi permanen pasien celah bibir dan palatum (nilai p = 0.156). Kesimpulan: Sel stromal pulpa gigi sulung dan gigi permanen memiliki kemampuan diferensiasi osteogenik karena dapat mengekspresikan marker osteogenik ALP.

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Background: Cleft and lip palate is a condition where there is fusion disturbance or abnormal congenital cleft in the upper lip, alveolar, and palate area that can cause problems in patients such as aesthetic disorder and problem with talking. Autologous bone graft reconstruction treatment is the gold standard in treating cleft lip and palate patients, but this treatment has associated shortcomings so that alternative treatments such as tissue engineering techniques have been developed. The source of the mesenchymal stromal cells used can be derived from dental pulp tissue namely stem cells from human deciduous teeth and permanent dental pulp stromal cells. The osteogenic differentiation ability from dental pulp stromal cells of primary and permanent teeth in cleft lip and palate patients is one of the considerations for the use of autologous cells in the treatment of tissue engineering techniques, while the osteogenic differentiation ability of dental pulp stromal cells in cleft lip and palate patients has not been fully explored.

Objective: To compare the osteogenic differentiation capacity of primary and permanent dental pulp stromal cells in cleft lip and palate patients.

Methods: Samples isolated from primary and permanent dental pulp stromal cells in cleft lip and palate patients were cultured, RNA were extracted and tested by Real-Time Polymerase Chain Reaction (RT PCR) using alkaline phosphatase primers (ALP), and housekeeping gene in the form of 18s. Results: The relative expression of ALP in primary dental pulp stromal cells in cleft lip and palate patients was comparable to permanent dental pulp stromal cells in cleft lip and palate patients (p value = 0.156).

Conclusion: The primary and permanent dental pulp stromal cells have comparable ability to differentiate into osteogenic lineage and both cells tested can express the osteogenic gene of ALP."

"Penelitian ini dilakukan untuk mengetahui bahwa ekstrak etanol rimpang temu mangga dapat berpengaruh terhadap penurunan kadar ALP serum darah tikus yang diinduksi karbon tetraklorida (CCl4). Hewan uji yang digunakan adalah tikus jantan galur Sprague-Dawley sebanyak 30 ekor yang dibagi dalam 6 kelompok perlakuan yaitu KK1, KK2, KP1, KP2, KP3 dan KP4. Tikus KK1 merupakan kelompok kontrol yang diinduksi akuades sedangkan kelompok KK2, KP1, KP2, KP3 dan KP4 merupakan kelompok yang diinduksi CCl4 dosis 1 ml/kg BB. Kemudian, kelompok KP1, KP2, KP3 dan KP4 diberikan ekstrak etanol rimpang temu mangga dosis 10 mg/kg BB, 20 mg/kg BB, 40 mg/kg BB dan 80 mg/kg BB sebanyak 4 kali dengan selang waktu 12 jam. Berdasarkan hasil penelitian, terjadi penurunan kadar ALP serum pada kelompok tikus KP1, KP2, KP3 dan KP4 secara berturut-turut sebesar 37,60%, 39,18%, 35,7% dan 33,75% jika dibandingkan dengan kadar ALP serum tikus yang diinduksi CCl4 (KK2). Dosis 20 mg/kg BB merupakan dosis yang paling optimal karena berdasarkan hasil uji LSD kelompok tersebut tidak memiliki perbedaan dengan KK1 atau dengan kata lain kadar ALP kelompok tersebut sudah mencapai kadar normal.

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The research aimed to find out that ethanol extract of mango ginger rhizome could affect the decrease of rat serum alkaline phosphatase (ALP) level that was induced by carbon tetrachloride (CCl4). Tested animals were 30 individuals of male Sprague-Dawley rats that were divided into six groups, namely KK1, KK2, KP1, KP2, KP3 and KP4. KK1 was a control group that was induced by aquades while KK2, KP1, KP2, KP3 and KP4 were groups that were induced by CCl4 dose of 1 ml/kg BW. Then, KP1, KP2, KP3 and KP4 were given the ethanol extract of mango ginger rhizome dose of 10 mg/kg BW, 20 mg/kg BW, 40 mg/kg BW and 80 mg/kg BW orally and administrated for 4 times with an interval of 12 hours. Based on the result, the decrease of rat serum Alkaline Phosphatase (ALP) level in KP1, KP2, KP3 and KP4 amounted to 37,48%, 39,17%, 36,79% and 36,09% compared to serum ALP level that was induced by CCl4 (KK2). Dose of 20 mg/kg BW is the most optimal dose since based on LSD test, this group has no difference with KK1 or in other words, ALP level of this group has reached normal level."

"ABSTRAKIdentifikasi fase pacu tumbuh pubertas penting untuk meningkatkan efisiensi dan efektifitas perawatan maloklusi. Tujuan penelitian ini untuk menganalisis potensi kadar Bone-Specific Alkaline Phosphatase BALP saliva dan parameter klinis sebagai prediktor fase pacu tumbuh pubertas dengan memperhatikan berbagai faktor tumbuh kembang skeletal. Penelitian diagnostik dengan sampel 136 orang ini menggunakan metode Cervical Vertebrae Maturation System CVMS Bacceti sebagai baku emas. Melalui analisis regresi multinomial dihasilkan model prediksi dengan nilai sensitifitas 78 untuk fase pra-puncak dan 57,7 untuk fase puncak, sedangkan nilai spesifisitas fase pasca-puncak 81,4 . Fase pacu tumbuh pubertas terutama pra-puncak dan pasca-puncak dapat diprediksi menggunakan kadar BALP saliva dan parameter klinis.

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ABSTRACTThe identification of growth spurt phase of puberty is important as it enhances the efficiency and effectiveness of malocclusion treatment. The objective of this study was to analyse the potential of the level of salivary Bone Specific Alkaline Phosphatase BALP and clinical parameters as a predictor of growth spurt phase of puberty by taking into account various factors affecting skeletal growth. The diagnostic test with the sample of 136 people was conducted by using the method of Cervical Vertebrae Maturation System CVMS from Bacceti as the gold standard. Multinomial regression analysis produced predictive models with 78 sensitivity at the pre peak phase and 57.7 at the peak phase, whereas the specificity of post peak phase was 81.4 . The growth spurt phase of puberty especially at the phases of pre peak and post peak can be predicted by using salivary BALP level and clinical parameters."

"ABSTRAK

Telah dilakukan penelitian yang bertujuan mengetahui potensi hepatoprotektif madu PS terhadap kadar alkali fosfatase (ALP) mencit (Mus musculus L.) jantan galur DDY. Dua puluh empat ekor mencit jantan dibagi ke dalam 4 kelompok hewan uji, yaitu kelompok kontrol normal (KK1) yang diberikan akuades dan minyak kelapa; kelompok kontrol perlakuan (KK2) yang diberikan akuades dan CCl4; serta 2 kelompok perlakuan (KP1 dan KP2) yang diberikan madu PS 10% dan 20% selama 14 hari berturut-turut, kemudian CCl4 2 jam setelah pemberian madu terakhir. Darah diambil 24 jam setelah injeksi CCl4. Kadar ALP diukur dengan metode kolorimetri. Hasil uji anova satu arah (P<0,05) menunjukkan adanya pengaruh nyata pemberian madu PS terhadap kadar ALP semua hewan uji. Dibandingkan kadar ALP KK2, kadar ALP KP1 lebih rendah 30,5% dan KP2 lebih rendah 52,9%. Namun, uji LSD (P<0,05) menunjukkan hanya kadar ALP KP2 yang tidak berbeda nyata dengan KK1. Berdasarkan hasil tersebut, disimpulkan bahwa potensi hepatoprotektif madu PS 20% lebih besar dibandingkan madu PS 10%.

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ABSTRACT

The study has been conducted to know the hepatoprotective potency of PS honey administration on male-DDY mice’s alkaline phosphatase level of blood plasma. Twenty four male mice were divided into four groups, namely normal control group (KK1) which was administered with aquadest and coconut oil; treatment control group (KK2) which was administered with aquadest and CCl4; and two treatment groups which was administered with PS honey 10% (KP1) and 20% (KP2) within 14 consecutive days and three groups (KK2, KP1,and KP2) were injected with CCl4 on the 14th day. Alkaline phosphatase was measured based on colorimetry method. One-way anova test (P<0,05) showed that alkaline phosphatase levels were significantly different. Compared with KK2, the alkaline phosphatase levels of KP1 and KP2 were 30,5% and 52,9% lesser than KK2, consecutively. However, LSD test (P<0,05) showed that only alkaline phosphatase level of KP2 was not significantly different. In conclusion, dose 20% of PS honey is more potential on hepatoprotective than those of 10%.

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"Penyakit hati merupakan penyakit yang dapat disebabkan oleh beberapa hal, salah satunya radikal bebas. Radikal bebas dapat menyerang membran sel hati (hepatosit), menyebabkan terjadinya peroksidasi lipid dan berujung pada kerusakan pada hepatosit. Kerusakan hati dapat dilihat dari meningkatnya kadar enzim alkali fosfatase pada serum. Pemberian infusa daun bertujuan untuk mengobati kerusakan hati, karena daun sukun memiliki kandungan flavonoid yang diduga berperan sebagai antioksidan. Penelitian yang dilakukan bertujuan untuk melihat potensi kuratif infusa daun sukun untuk mengobati kerusakan hati. Hewan uji yang digunakan adalah tikus jantan galur Sprague Dawley yang dibagi dalam 5 kelompok perlakuan yakni KK1, KK2, KP1, KP2, dan KP3. Tikus diinduksi dengan karbon tetraklorida (CCl4) dosis 280 mg/kg BB, kemudian diberikan infusa daun sukun untuk KP1, KP2, dan KP3 secara berturut-turut dengan dosis 2,7; 5,4; dan 10,8 g/kg BB sebanyak 4 kali dengan selang waktu 12 jam. Berdasarkan hasil penelitian, terjadi penurunan kadar ALP serum pada tikus KP1, KP2, dan KP3 secara berturut-turut sebesar 20,66%, 26,45%, dan 33,89% jika dibandingkan dengan kadar ALP serum tikus yang diinduksi CCl4 (KK2). Dosis 10,8 g/kg BB merupakan dosis yang memberikan penurunan kadar ALP yang paling mendekati kadar normal.

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Liver disease is one disease that can be caused by several things, one of which is free radicals. Free radicals can attack the cell membrane of the hepatocytes, causing lipid peroxidation and result in damage to the hepatocytes. Liver damage can be seen from the elevated alkaline phosphatase levels in serum. Administration of breadfruit leaves infusion aims to treat liver damage, as breadfruit leaf contains flavonoids which allegedly acted as an antioxidant. Research carried out is to look at the ability of breadfruit leaves infusion to treat liver damage. Tested animals were Sprague Dawley strain male rats divided into five groups namely KK1, KK2, KP1, KP2 and KP3. Rats induced by carbon tetrachloride (CCl4) dose of 280 mg/kg, then given the breadfruit leaves infusion for KP1, KP2 and KP3 respectively at a dose of 2.7; 5.4; and 10.8 g kg 4 times with an interval of 12 hours. Based on the results of the study, decreased of serum ALP levels in KP1, KP2 and KP3 rats amounted to 20.66%, 26.45%, and 33.89% when compared to CCl4 induced rats (KK2). Dose of 10.8 g/kg is the dose that gives the most reduction in ALP levels approaching normal levels."

"Latar belakang: Celah bibir dan palatum adalah kelainan bawaan yang mempengaruhi regio orofacial. Perawatan yang menjadi baku emas untuk pasien celah bibir dan palatum adalah autologous bone graft. Namun, perawatan ini masih invasif dan ada beberapa kekurangannya sehingga perlu teknik rekayasa jaringan dengan sel stromal. Sel stromal mesenkim yang terdapat dalam rongga mulut adalah sel stromal pulpa gigi sulung (SHED) dan sel stromal pulpa gigi permanen (DPSC). Kemampuan diferensiasi osteogenik SHED dan DPSC pada subjek normal sudah diketahui. Namun, kemampuan diferensiasi osteogenik dengan ekspresi gen RUNX-2 pada DPSC dan SHED pasien celah bibir dan palatum belum diketahui secara pasti. Tujuan: Membandingkan kemampuan diferensiasi osteogenik sel stromal pulpa gigi permanen pasien celah bibir dan palatum dengan sel stromal pulpa gigi sulung pasien celah bibir dan palatum melalui ekspresi gen RUNX-2. Metode: DPSC celah bibir dan palatum dan SHED celah bibir dan palatum dikultur dengan medium osteogenik dan tanpa medium osteogenik selama 21 hari. Sampel RNA diperoleh kultur sel stromal pulpa gigi permanen (DPSC) dan sel stromal pulpa gigi sulung (SHED) pasien celah bibir dan palatum. Selanjutnya diuji ekspresi gen RUNX-2, dan housekeeping gene 18S dengan Real-Time Polymerase Chain Reaction (RT-PCR). Hasil: Tidak ada perbedaan kemampuan diferensiasi sel stromal pulpa gigi permanen pasien celah bibir dan palatum dengan sel stromal pulpa gigi sulung pasien celah bibir dan palatum melalui ekspresi gen RUNX-2. Kesimpulan: Kemampuan diferensiasi osteogenik sel stromal pulpa gigi sulung pasien celah bibir dan palatum ekuivalen dengan sel stromal pulpa gigi permanen pasien celah bibir dan palatum.

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Background: Cleft lip and palate are congenital anomalies that affect the orofacial region including lips, alveolar ridge, hard palate, and soft palate. Patients with cleft lip and palate have impaired esthetic and stomatognathic functions. The gold standard treatment for cleft lip and palate patients is an autologous bone graft. However, this treatment is still invasive and has some limitations therefore requires tissue engineering techniques by using stromal cells. Mesenchymal stromal cells that are found in the mouth are stromal cells from human exfoliated deciduous teeth (SHED) and dental pulp stromal cells (DPSC). The osteogenic differentiation of SHED and DPSC normal subjects are well known. Nevertheless, the osteogenic differentiation capacity by RUNX-2 mRNA expression in DPSC and SHED cleft lip and palate patients is still need to be elucidated. Objective: To compare the osteogenic differentiation capacity of stromal cells from human exfoliated deciduous teeth and dental pulp stromal cells in cleft lip and palate patients through RUNX-2 gene expression. Methods: DPSC and SHED cleft lip and palate patients were cultured with and without osteogenic medium for 21 days. RNA sample were collected from cell culture followed by the examination of RUNX-2 and 18S gene expression were tested by Real-Time Polymerase Chain Reaction (RT-PCR). Result: There was no difference in osteogenic differentiation capacity between DPSC and SHED cleft lip and palate patients through RUNX-2 gene expression. Conclusion: The osteogenic differentiation capacity of SHED was equivalent to DPSC of cleft lip and palate patients."

"Latar belakang: Celah bibir dan palatum (CLP) adalah kegagalan fusi prosesus frontonasal dan maksilaris yang menghasilkan celah yang meluas ke bibir, alveolus, dasar hidung, dan palatum keras maupun lunak. Diperlukan perawatan melalui teknik rekayasa jaringan dengan menggunakan sel stromal mesenkim yang dapat ditemukan pada dental pulp stromal cells (DPSC). Kemampuan osteogenik DPSC dapat dilihat melalui deteksi marker osteogenik seperti osteopontin (OPN). Osteopontin merupakan salah satu marker utama diferensiasi osteogenik yang diproduksi oleh osteoblas dalam proses pembentukan dan mineralisasi tulang. Pada pasien CLP diketahui perbedaan ekspresi gen yang dapat memengaruhi sintesis matriks ekstraseluler. Penelitian ini dilakukan untuk mengetahui kemampuan diferensiasi osteogenik melalui ekspresi marker osteopontin saat diferensiasi osteoblas pada pasien celah bibir dan palatum. Tujuan: Membandingkan kemampuan diferensiasi osteogenik sel stromal pulpa gigi subjek normal dengan sel stromal pulpa gigi pasien celah bibir dan palatum melalui ekspresi gen osteopontin. Metode: Sampel RNA yang diperoleh dari kultur sel pulpa gigi subjek normal dan pasien celah bibir dan palatum diuji dengan Real-Time Polymerase Chain Reaction (RT-PCR) dengan primer osteopontin (OPN) dan 18S sebagai housekeeping gene. Hasil: Tidak terdapat perbedaan antara ekspresi relatif gen OPN sel stromal pulpa gigi subjek normal dan pasien celah bibir dan palatum. Kesimpulan: Kemampuan diferensiasi osteogenik sel stromal pulpa gigi pada pasien celah bibir dan palatum ekuivalen dengan sel stromal pulpa gigi pada subjek normal.

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Background: Cleft lip and palate (CLP) occurs due to the failure of fusion of the frontal and maxillary process that results in a cleft that extends to the lip, alveoli, nose floor, and hard and soft palate. One of the potentially alternative treatment for CLP cases is tissue engineering technique using Mesenchymal Stromal Cell (MSC). MSC can be found in dental pulp stromal cells (DPSC). Osteogenic ability can be seen through the detection of osteogenic markers such as osteopontin (OPN). Osteopontin is one of the main markers of osteogenic differentiation produced by osteoblast in the process of bone formation and mineralization. Osteopontin is expressed by preosteoblasts in early bone formation and mature osteoblasts at bone remodelling sites. Osteopontin expression as one of osteogenic markers in cleft lip and palate patients is unknown. This study was conducted to determine the ability of osteogenic differentiation through the expression of osteopontin in cleft lip and palate patients. Objective: To compare osteogenic differentiation ability of mesenchymal stromal cells in cleft lip and palate patients and normal subject through the expression of osteogenic marker osteopontin. Methods: RNA sample that was obtained through RNA extraction from dental pulp stromal cells of cleft and lip palate patients and normal subjects were tested with Real-Time Polymerase Chain Reaction (RT-PCR) using osteopontin and 18S as housekeeping gene. Results: There was no difference between the relative expression of OPN gene in DPSC from normal subject and cleft lip and palate patients. Conclusion: Osteogenic differentiation ability of dental pulp stromal cells from cleft lip and palate patients is equivalent with dental pulp stromal cells from normal subjects."

"Latar Belakang: Subjek celah bibir dan palatum membutuhkan perawatan rekonstruksi tulang berbasis rekayasa jaringan dengan menggunakan sel stromal mesenkim. Sel stromal mesenkim merupakan sel yang banyak digunakan untuk regenerasi tulang karena mempunyai kemampuan proliferasi tinggi. Sel tersebut dapat berasal dari pulpa gigi sulung (SHED) danpulpa gigi permanen (DPSCs) yang dapat berdiferensiasi menjadi osteoblas. Pada penelitiansebelumnya telah ditemukan beberapa karakteristik DPSCs dan SHED pada subjek celah bibir dan palatum, namun kemampuan diferensiasi dari sel stromal pulpa subjek celah bibir dan palatum belum diketahui. Tujuan: Mengevaluasi kemampuan diferensiasi osteogenik dari sel stromal pulpa gigi permanen dan sulung pada subjek celah bibir dan palatum melaluiekspresi gen Collagen Type I Alpha I (COL1A1). Metode : Sampel RNA yang diperoleh dari kultur RNA DPSCs dan SHED subjek celah bibir dan palatum, dengan Real-Time Polymerase Chain Reaction (RT-PCR) menggunakan primers Collagen Type I Alpha I (COL1A1), serta 18S sebagai housekeeping gene. Hasil : Tidak terdapat perbedaan ekspresi relatif gen COL1A1 antara sel stromal pulpa gigi permanen dan sel stromal pulpa gigi sulung pada subjek celah bibir dan palatum. Kesimpulan : SHED memiliki kemampuan diferensiasi osteogenik yang sama dengan DPSCs karena keduanya dapat mengekspresikan gen marker osteogenik COL1A1.

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Background: Cleft lip and palate subject need bone reconstruction based tissue engineering treatment with mesenchymal stromal cells (MSC). One of the most mesenchymal stromal cells that can be used is derived from dental pulp tissues, such as primary tooth pulp or stem cells from human deciduous teeth (SHED) and dental pulp stem cells (DPSCs) which can differentiate into osteoblasts. In previous studies, several characteristics of DPSCs and SHED of the cleft lip and palate subjects have been found. However, osteogenic differentiation ability of dental pulp stromal cells from cleft lip and palate subject is unknown.

Objective: To determine the osteogenic differentiation ability of DPSCs and SHED of cleft lip and palate subjects through the expression of the Collagen Type I Alpha I (COL1A1) gene.

Methods: RNA samples obtained from the culture of DPSCs and SHED of lip and palate cleft subjects, with Real-Time Polymerase Chain Reaction (RT-PCR) using primers Collagen Type I Alpha I (COL1A1) and 18S as a housekeeping gene.

Results: There was no difference in the relative expression of COL1A1 gene between DPSCs and SHED of CLP subjects.

Conclusion: SHED has the same osteogenic differentiation ability as DPSCs because they can express osteogenic marker genes COL1A1."

"Latar Belakang: Rekayasa jaringan merupakan alternatif untuk perawatan rekonstruksi tulang alveolar pasien celah bibir dan palatum. Alternatif tersebut menghubungkan penggunaan sel punca, biomaterial/scaffolds, dan molekul sinyal. Sumber sel yang ideal untuk rekayasa jaringan adalah sel autologous karena tidak bersifat immunogenik. Sel stromal pulpa gigi permanen (DPSC) menarik untuk terapi klinis karena akses perolehannya yang mudah, morbiditas yang sangat rendah, menunjukkan kapasitas imunoregulasi yang menguntungkan, dan dapat berdiferensiasi menjadi banyak tipe sel, termasuk osteoblas. Pada penelitian sebelumnya, DPSCs pasien celah bibir dan palatum ditemukan memiliki potensi kemampuan osteogenik. Namun, kemampuan diferensiasi osteogeniknya belum diketahui. Kemampuan diferensiasi osteogenik tersebut dapat diamati dari ekspresi marker osteogenik, salah satunya sclerostin yang diekspresikan pada tahap akhir diferensiasi osteoblas. Tujuan: Membandingkan kemampuan diferensiasi osteogenik DPSCs pasien celah bibir dan palatum dengan DPSCs subjek normal melalui pengamatan ekspresi gen sclerostin. Metode: DPSCs dikultur hingga mencapai 70%-80% confluent. Sampel RNA dari sel diperoleh dengan melakukan prosedur ekstraksi RNA. Ekspresi gen sclerostin diamati menggunakan Real-Time PCR menggunakan primer sclerostin dan 18s sebagai housekeeping gene. Hasil: DPSCs pasien celah bibir dan palatum memiliki nilai rata-rata ekspresi relatif gen sclerostin yang lebih tinggi 1,9 kali lipat dibandingkan dengan DPSCs subjek normal dan secara statistik berbeda bermakna dengan p = 0,013. Kesimpulan: DPSCs pada pasien celah bibir dan palatum mengekspresikan gen sclerostin sebagai marker diferensiasi osteogenik yang lebih tinggi dibandingkan DPSCs pada subjek normal secara in vitro.

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Background: Tissue engineering is an alternative for alveolar bone reconstruction treatment in cleft lip and palate (CLP) patients. The alternative links the use of stem cells, biomaterials/scaffolds, and signaling molecules. The ideal cell source for tissue engineering is autologous cells because they are not immunogenic. Dental pulp stromal cells (DPSC) are interesting for clinical therapy because of their easy accesses, very low morbidity, exhibit favorable immunoregulatory capacities, and can differentiate into many cell types, including osteoblasts. In a previous study, DPSCs in CLP patients were found to have a potential osteogenic ability. However, its osteogenic differentiation ability is not yet known. The ability of osteogenic differentiation can be observed from the expression of osteogenic markers, one of which is sclerostin, a marker that is expressed in the final stage of osteoblast differentiation. Objective: To compare osteogenic differentiation ability of DPSCs in CLP patients with DPSCs in normal subjects through the expression of sclerostin gene. Methods: DPSCs were cultured to reach 70%-80% confluent. RNA samples from cells were obtained by carrying out RNA extraction procedure. Sclerostin gene expression was assessed using Real-Time PCR using sclerostin primer and 18s as a housekeeping gene. Results: DPSCs from CLP patients have mean relative expression of sclerostin gene 1.9 times higher compared to DPSCs in normal subjects and it is statistically different with p = 0.013. Conclusions: DPSCs in CLP patients express the sclerostin gene as marker of osteogenic differentiation higher than DPSCs in normal subjects in vitro."