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"ABSTRAKIkan kancra merupakan salah satu spesies air tawar di Indonesia yang populasinya terus terancam. Upaya yang dapat dilakukan untuk melestarikan material genetik hewan yang terancam punah yaitu kriopreservasi spermatozoa. Salah satu faktor yang menentukan keberhasilan kriopreservasi adalah krioprotektan. Salah satu krioprotektan alami potensial yaitu kuning telur puyuh. Tujuan dari penelitian ini yaitu mengevaluasi konsentrasi optimal dari berbagai konsentrasi kuning telur puyuh (0%, 5%, 10%, 15%, 20%, dan 25%) terhadap motilitas, viabilitas, abnormalitas dan kemampuan fertilisasi spermatozoa ikan kancra 48 jam pascakriopreservasi. Rasio antara semen dan larutan pengencer yang digunakan yaitu 1:10. Analisis data menggunakan uji ANAVA satu arah dan dilanjutkan dengan uji Tukey. Berdasarkan hasil uji ANAVA, pemberian berbagai konsentrasi kuning telur puyuh yang dikombinasikan dengan metanol 10% menghasilkan pengaruh nyata (P<0,05) terhadap persentase motilitas, viabilitas, abnormalitas, dan fertilitas spermatozoa pascakriopreservasi. Kuning telur 10% merupakan konsentrasi optimal yang dapat mempertahankan persentase motilitas, viabilitas, dan fertilitas tertinggi yaitu masing-masing 85,10 ± 1,51%, 84,75 ± 1,71% dan 95,00 ± 1,83% serta menghasilkan nilai persentase abnormalitas terendah yaitu 20,25 ± 1,71%.

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ABSTRACTKancra fish is one of the freshwater species in Indonesia, whose population is continues to be threatened. The efforts that can be done to preserve the endangered animal's genetic material are cryopreservation of spermatozoa. One of the factors that determines the success of cryopreservation is cryoprotectant. One of the potential natural cryoprotectants is quail egg yolks. The purpose of this research is to evaluate the optimal concentration of various concentrations of quail egg yolks (0%, 5%, 10%, 15%, 20%, and 25%) on motility, viability, abnormality and fertilization ability of kancra fish spermatozoa 48 hours post-cryopreservation. The ratio between semen and diluent solution used was 1:10. Data was analyzed using one-way ANOVA test and continued with the Tukey test. Based on the results of the one-way ANOVA test, it was found that cryoprotectant of quail egg yolk combined with 10% methanol had significant effect (P <0.05) on motility, viability, abnormality, and fertility of spermatozoa post-cryopreservation. The 10% of quail egg yolk concentration was the optimal concentration that could maintain the highest percentage of motility, viability and fertility, respectively 85.10 ± 1.51%, 84.75 ± 1.71%, 95.00 ± 1.83% and produced the lowest percentage value of abnormality 20.25 ± 1.71%."

"ABSTRAKKriopreservasi merupakan metode penyimpanan materi genetik pada suhu yang rendah dalam jangka waktu yang lama. Manfaat kriopreservasi salah satunya untuk mengatasi masalah penurunan populasi. Ikan kancra merupakan salah satu spesies dari famili Cyprinidae yang populasinya semakin menurun sehigga diperlukan upaya pelestarian. Faktor yang mempengaruhi keberhasilan kriopreservasi salah satunya adalah krioprotektan. Krioprotektan alami digunakan karena memiliki toksisitas yang lebih rendah dibanding krioprotektan dari bahan kimia. Kuning telur merupakan krioprotektan alami yang dapat menjaga sel selama proses kriopreservasi. Tujuan dari penelitian yaitu mengevaluasi efek kuning telur ayam kampung sebagai krioprotektan alami terhadap motilitas, viabilitas, abnormalitas dan fertilisasi spermatozoa 48 jam pascakriopreservasi. Konsentrasi kuning telur ayam kampung yang digunakan yaitu: 0%, 5%, 10%, 15%, 20% dan 25%. Rasio yang digunakan untuk pengenceran sperma yaitu 1:10. Analisis data menggunakan uji ANAVA kemudian dilanjutkan dengan uji Tukey. Berdasarkan hasil uji ANAVA satu arah diketahui bahwa krioprotektan kuning telur ayam kampung yang dikombinasikan dengan metanol 10% memberikan pengaruh (P<0,05) terhadap motilitas, viabilitas, abnormalitas dan fertilitas spermatozoa pascakriopreservasi. Konsentrasi kuning telur 5% merupakan konsentrasi optimum yang dapat mempertahankan persentase motilitas, viabilitas dan fertilitas tertinggi masing-masing 84,06 ± 1,67%, 82,13 ± 1,75% dan  92,96 ± 1,94% serta nilai persentase abnormalitas terendah 25,25 ± 2,22%.

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ABSTRACTCryopreservation is a method of storing genetic material at low temperatures for long periods of time. One of the benefits of cryopreservation is to overcome the problem of population decline. The kancra fish is one of the species of the Cyprinidae family whose population is declining so that conservation efforts are needed. One of the factors that influence the success of cryopreservation is cryoprotectant. Natural cryoprotectants are used because they have lower toxicity than cryoprotectants from chemicals. Egg yolk is a natural cryoprotectant that can maintain cells during the cryopreservation process. The purpose of this study is to evaluate the effect of egg yolk as natural cryoprotectants on motility, viability, abnormalities, and fertilization of 48-hour spermatozoa after cryopreservation. Concentrations of free-range chicken egg yolk used are: 0%, 5%, 10%, 15%, 20% and 25%. The ratio used for sperm dilution is 1:10. Data analysis using the ANAVA test and then followed by the Tukey test. Based on the results of the one-way ANAVA test, it was found that cryoprotectant of free-range chicken egg yolk combined with 10% methanol had an effect (P <0.05) on motility, viability, abnormality, and fertility of post-cryoprotected spermatozoa. Egg yolk concentration of 5% was the optimum concentration that can maintain the highest percentage of motility, viability and fertility, respectively 84.06 ± 1.67%, 82.13 ± 1.75% and 92.96 ± 1.94% and the percentage value the lowest abnormality was 25.25 ± 2.22%."

"ABSTRAKKriopreservasi merupakan metode penyimpanan material genetik pada suhu rendah dalam jangka waktu tertentu. Kriopreservasi spermatozoa dapat digunakan sebagai usaha konservasi ex situ ikan kancra (Tor soro) yang populasinya semakin menurun. Pemilihan krioprotektan yang tepat dapat meminimalkan kerusakan akibat terbentuknya kristal es dan mempertahankan kualitas spermatozoa selama kriopreservasi. Gula merah sebagai krioprotektan alami dan metanol 10% memiliki potensi untuk kriopreservasi spermatozoa ikan kancra. Tujuan penelitian yaitu mendapatkan konsentrasi optimal dari berbagai konsentrasi gula merah dan metanol 10% terhadap motilitas, viabilitas, dan abnormalitas spermatozoa pascakriopreservasi ikan kancra, serta mengevaluasi persentase fertilitas spermatozoa pascakriopreservasi ikan kancra. Konsentrasi gula merah yang digunakan yaitu 0%, 5%, 10%, 15%, 20%, dan 25%. Rasio antara semen dan larutan pengencer yang digunakan yaitu 1:10. Ekuilibrasi dilakukan pada suhu 5 °C selama 10 menit. Pembekuan dilakukan pada suhu -10 °C selama 48 jam. Pencairan dilakukan pada suhu 40 °C selama 60 detik. Analisis data dilakukan dengan menggunakan uji ANAVA yang dilanjutkan dengan uji Tukey. Hasil penelitian menunjukkan bahwa krioprotektan gula merah memberikan pengaruh (P<0,05) pada kualitas spermatozoa pascakriopreservasi. Krioprotektan gula merah 15% dan metanol 10% menghasilkan persentase motilitas (81,85 ± 1,11%), viabilitas (83,75 ± 1,71%), fertilitas (89,75 ± 1,71%) tertinggi, serta abnormalitas terendah (14,50 ± 1,73%) pada spermatozoa pascakriopreservasi.

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ABSTRACTCryopreservation is a method of storing genetic material at low temperatures for a certain period of time. Spermatozoa cryopreservation can be used as an ex situ conservation method for kancra fish (Tor soro) whose population is declining. Selection of the right cryoprotectant can minimize damage caused by the formation of ice crystals and maintain the quality of spermatozoa during cryopreservation. Brown sugar as natural cryoprotectant and 10% methanol have potential for cryopreservation of spermatozoa. The research objective was to obtain the optimal concentration of various concentrations of brown sugar and 10% methanol on the motility, viability, and abnormalities of kancra fish spermatozoa after cryopreservation, and evaluate the fertility of kancra fish spermatozoa after cryopreservation. The concentration of brown sugar used were 0%, 5%, 10%, 15%, 20%, and 25%. The ratio between semen and diluent solution used was 1:10. Equilibration was carried out at 5 °C for 10 minutes. Freezing was carried out at -10 °C for 48 hours. Thawing was done at 40 °C for 60 seconds. Data was analyzed using ANOVA test followed by Tukey test. The results showed that the cryoprotectant brown sugar had an significant influence (P<0.05) on the quality of post-cryopreserved spermatozoa. 15% brown sugar together with 10% methanol can produce the highest percentage of motility (81.85 ± 1.11%), viability (83.75 ± 1.71%), fertility (89.75 ± 1.71%), and the lowest abnormality (14.50 ± 1.73%) in spermatozoa post-cryopreservation."

"ABSTRAKIkan kancra (Tor soro) merupakan salah satu spesies dari Genus Tor yang mengalami penurunan populasi karena kegiatan eksploitasi dan kendala pematangan gonad. Kriopreservasi dapat dijadikan sebagai cara untuk mengatasi penurunan populasi dan menjaga kelestarian dari T. soro. Keberhasilan dari kriopreservasi dipengaruhi oleh beberapa faktor, salah satunya krioprotektan. Krioprotektan yang digunakan pada penelitian ini yaitu sari kedelai dan metanol. Sari kedelai memiliki lesitin yang mampu melindungi bagian luar sel, sedangkan metanol memiliki berat molekul yang kecil sehingga mampu menembus membran sel dan melindungi bagian dalam sel. Penelitian ini bertujuan untuk mengevaluasi pengaruh krioprotektan sari kedelai dengan berbagai konsentrasi (0%, 5%, 10%, 15%, 20%, dan 25%) terhadap motilitas, viabilitas, abnormalitas, dan fertilitas spermatozoa T. soro 48 jam pascakriopreservasi pada suhu -10 oC. Analisis data menggunakan uji ANAVA satu arah dan dilanjutkan dengan uji Tukey. Hasil penelitian menunjukkan bahwa penambahan sari kedelai berbagai konsentrasi dan metanol 10% memberikan pengaruh nyata terhadap persentase motilitas dan viabilitas spermatozoa T. soro 48 jam pascakriopreservasi (P<0,05). Sari kedelai 5% dan metanol 10% merupakan kombinasi krioprotektan dengan konsentrasi optimum dalam mempertahankan persentase motilitas (84,37 ± 1,54%), viabilitas (78,00 ± 2,37%), dan fertilitas (97,50 ± 1,91%), serta menghasilkan persentase abnormalitasterendah (23,00 ± 2,58%) pada spermatozoa T. soro 48 jam pascakriopreservasi.

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ABSTRACTKancra fish (Tor soro) is a species of the Genus Tor that has decreased population due to exploitation activities and gonad maturation problems. Cryopreservation is a way to overcome population decline and preserve T. soro resources. The success of cryopreservation is influenced by several factors, one of which is cryoprotectant. Cryoprotectants used in this study were soybean milk and methanol. Soybean milk has lecithin which can protect the outside part of cells, while methanol has a small molecular weight so that it can penetrate the cell membranes and protect the inner part of cells. This study aimed to evaluate the effects of soybean milk cryoprotectants in various concentrations (0%, 5%, 10%, 15%, 20%, and 25%) on motility, viability,abnormality, and fertility of T. soro spermatozoa 48 hours post-cryopreservation at -10oC. Data analysis used a one-way ANOVA test and followed by the Tukey test. The results showed that the addition of soybean milk in various concentrations and 10% methanol had a significant effect on the motility and viability percentage of T. sorospermatozoa 48 hours post-cryopreservation (P<0.05). The 5% soybean milk and 10% methanol were cryoprotectant combination with optimum concentrations in maintaining the motility percentage (84.37 ± 1.54%), viability percentage (78.00 ± 2.37%), fertility percentage (97.50 ± 1.91%), and produced the lowest abnormality percentage (23.00 ±2.58%) of T. soro spermatozoa 48 hours post-cryopreservation."

"Tor soro adalah ikan endemik yang ditemukan di beberapa pulau di Indonesia meliputi Jawa, Sulawesi, dan Kalimantan. Ikan kancra Tor soro) mengalami penurunan jumlah di alam karena adanya overfishing,rusaknya habitat dan waktu pematangan kematangan gonad jantan dan betina yang berbeda. Upaya yang dapat dilakukan untuk menanggulangi hal salah satunya dengan melakukan kriopreservasi materi genetik (spermatozoa). Telah dilakukan penelitian mengenai kriopreservasi ikan kancraTor soromenggunakan berbagai konsentrasi sari buah anggur Vitis vinifer sebagai antioksidan alami yang dikombinasikan dengan DMSO 10%. Tujuan penelitian adalah untuk mengetahui pengaruh berbagai konsentrasi sari buah anggur (KK 0%; KP1 10%; KP2 20%; dan KP3 30%) yang dikombinasikan dengan DMSO 10% terhadap kualitas spermatozoa dan kemampuan fertilisasi spermatozoa ikan kancra 24 jam pascakriopreservasi. Hipotesis penelitian adalah sari buah anggur dengan berbagai konsentrasi (KK 0%; KP1 10%; KP2 20%; dan KP3 30%) memberikan pengaruh terhadap parameter uji meliputi motilitas, viabilitas, abnormalitas dan kemampuan fertilisasi spermatozoa ikan kancra 24 jam pascakriopreservasi. Hasil uji ANAVA satu faktor menunjukkan pemberian berbagai konsentrasi sari buah anggur memiliki rata-rata persentase motilitas, viabilitas, dan kemampuan fertilisasi spermatozoa ikan kancra 24 jam pascakriopreservasi berbeda yang nyata (P<0,05). Sedangkan pada rata-rata abnormalitas tidak ditemukan perbedaan (P>0,05). Hasil uji perbandingan berganda Tukey menunjukkan bahwa terdapat perbedaan yang nyata antarkelompok perlakuan KK (Sari buah anggur 0%) dengan KP1 (Sari buah anggur 10%) terhadap persentase motilitas, viabilitas dan kemampuan fertilisasi spermatozoa ikan kancra 24 jam pascakriopreservasi. Kombinasi terbaik adalah DMSO 10% dan sari buah anggur 10% karena dapat mempertahankan nilai persentase motilitas, viabilitas, kemampuan fertilisasi tertinggi dan nilai peresentase abnormalitas terendah dengan nilai 41,8 ± 11,2 %; 31,7%± 10,1 %; 77,8% ± 1,7 % ; dan 77,8% ± 1,7 %.Tor soro is an endemic fish found on several islands in Indonesia including Java, Sulawesi and Kalimantan. Kancra fishTor sorohas decreased in number due to overfishing, habitat destruction and asynchronous maturation timr of male and female gonad. Efforts that can be made to overcome this problems are cryopreserving of genetic material (spermatozoa). Cryopreservation of kancra fish Tor soro using various concentrations of grape juice (Vitis vinifera) as a natural antioxidant combined with 10% DMSO has been studied. The objective of this study was to determine the effect of grape juice (KK 0%; KP1 10%; KP2 20%; and KP3 30%)  combined with 10% DMSO on spermatozoa quality and fertilitation rate of spermatozoa kancra fish 24 hours postriopreservation. The hypothesis is grape juice concentration (KK 0%; KP1 10%; KP2 20%; and KP3 30%) influences the test parameters including motility, viability, abnormality and fertilization rates of kancra fish spermatozoa 24 hours postcryopreservation. The results of the one factor ANAVA test showed that giving various concentrations of grape juice had an average percentage of motility, viability, and fertilization rates of spermatozoa for 24 hour cryopreservation significantly different (P <0.05). While the abnormalities were found no difference (P> 0.05). The results of Tukey's multiple comparison test showed that there were significant differences between the control groups (0% grape juice) and KP1 (10% grape juice) to the percentage of motility, viability and fertilization ability of spermatozoa of kancra fish 24 hours postriopreservation. The best combination is DMSO 10% and grape juice 10% because it can maintain the highest percentage of motility, viability, fertilization ability and the lowest percentage of abnormalities with a values are 41,8 ± 11,2 %; 31,7%± 10,1 %; 77,8% ± 1,7 % ; and 77,8% ± 1,7 %."

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Upaya budi daya T. soro mengalami kesulitan akibat matang gonad jantan dan gonad betina terjadi tidak secara bersamaan. Kriopreservasi sperma T. soro dapat dimanfaatkan sebagai alternatif menyelesaikan masalah tersebut. Salah satu faktor yang memengaruhi keberhasilan proses kriopreservasi adalah penggunaan krioprotektan. Peneliti menggunakan krioprotektan metanol 10% dan krioprotektan berbahan dasar alami sari kurma dengan konsentrasi 0%, 5%, 10%, 15%, 20%, dan 25%. Tujuan penelitian adalah untuk mengevaluasi efek sari kurma sebagai krioprotektan alami dengan kombinasi metanol 10% terhadap kualitas sperma (motilitas spermatozoa, viabilitas spermatozoa, dan abnormalitas spermatozoa) serta persentase fertilitas T. soro 48 jam pascakriopreservasi. Rasio pengenceran yang digunakan adalah 1:10. Penyimpanan dilakukan pada freezer dengan suhu -10 °C selama 48 jam. Hasil uji ANAVA satu arah menunjukkan bahwa pemberian berbagai konsentrasi sari kurma dengan kombinasi metanol 10% berpengaruh nyata (P<0,05) terhadap viabilitas spermatozoa, abnormalitas spermatozoa, dan kemampuan fertilisasi spermatozoa 48 jam pascakriopreservasi. Metanol 10% dan sari kurma 10% merupakan kombinasi krioprotektan dengan konsentrasi optimum yang mampu mempertahankan persentase motilitas tertinggi 81.29 ± 1.01%, persentase viabilitas tertinggi 80.75 ± 1.19%, persentase abnormalitas terendah 21.5 ± 1.29%, serta persentase fertilitas tertinggi 88,50 ± 1,73% spermatozoa T. soro 48 jam pascakriopreservasi. 

 

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Tor soro cultivation has several obstacles including gonad synchronization. Tor soro cryopreservation of sperm can be used as an alternative to solve the problem. One of the success factors of cryopreservation is cryoprotectant. Researchers used a 10% methanol and date palm juice (0%, 5%, 10%, 15%, 20%, dan 25%) as cryoprotectant. The study aimed to evaluate the effects of date palm juice as a natural cryoprotectant with a combination of 10% methanol on sperm quality (spermatozoa motility, viability, and abnormality) and the percentage of fertility of T. soro 48 hours post-cryopreservation. The dilution ratio used was 1:10. Storage was carried out in the freezer at a temperature of -10 °C for 48 hours. The results of the one-way ANOVA test showed that various concentrations of date palm juice in combination with methanol 10% had a significant effect (P<0.05) on percentage of spermatozoa viability, spermatozoa abnormality, and spermatozoa fertility of 48 hours post-cryopreservation. The combination of 10% methanol and 10% date palm juice was the optimum concentration that was able to maintain the highest motility percentage 81.29 ± 1.01%, the highest viability percentage 80.75%  ± 1.19%, the lowest abnormality percentage 21.5 ± 1.29%, and the highest fertility percentage 88.50 ± 1.73%.

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"Kriopreservasi sperma pada ikan kancra (Tor soro Valenciennes, 1842) merupakan salah satu cara untuk mengatasi permasalahan yang berkaitan dengan waktu pemijahan yang berbeda antara induk jantan dan induk betina. Penelitian ini menggunakan metanol sebagai krioprotektan intraseluler dan susu skim sebagai krioprotektan ekstraseluler. Tujuan penelitian ini yaitu untuk mengevaluasi pengaruh metanol dan susu skim sebagai krioprotektan terhadap kualitas serta kemampuan fertilisasi sperma Tor soro pascakriopreservasi 48 jam. Krioprotektan yang digunakan dalam penelitian ini yaitu metanol 10 % dan susu skim dalam berbagai konsentrasi (5%; 10%; 15%; 20%; dan 25%). Rasio sperma dan larutan pengencer dalam penelitian ini yaitu 1:9. Ekstender yang digunakan yaitu larutan fish Ringer. Sperma disimpan dalam deep freezer pada suhu –34 ^oC selama 48 jam. Sperma segar dievaluasi terlebih dahulu untuk menguji kelayakan sperma untuk kriopreservasi. Sperma segar dievaluasi secara makroskopik (warna, volume sperma dan pH), secara mikroskopik (motilitas, viabilitas, dan abnormalitas), dan kemampuan fertilisasi dengan menghitung jumlah telur yang terfertilisasi. Sperma pascakriopreservasi 48 jam dievaluasi dengan cara mikroskopik dan dilihat kemampuan fertilisasi. Hasil penelitian menunjukkan bahwa krioprotektan metanol 10% dengan berbagai konsentrasi susu skim mempunyai pengaruh nyata pada beberapa konsentrasi (P<0,05) terhadap motilitas, viabilitas, abnormalitas dan kemampuan fertilisasi. Berdasarkan uji statistik one-way ANAVA yang dilanjutkan dengan uji Tukey. Penggunaan metanol 10% dengan susu skim 10% merupakan konsentrasi terbaik yang menghasilkan motilitas 82,90 ± 1,40%; viabilitas 79,00 ± 2,16%; abnormalitas terendah 27,75 ± 1,26% serta kemampuan fertilisasi 91,25 ± 2,21%.

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Cryopreservation sperm of kancra fish (Tor soro Valenciennes, 1842) is one of the solutions to overcome the problems related to different spawning times between male and female broodstock. This study used cryoprotectant methanol as intracellular cryoprotectant and skim milk as extracellular cryoprotectant. The purpose of this study was to evaluate the effect of methanol and skim milk as cryoprotectant on the quality and ability of sperm fertilization of Tor soro 48 hours post-cryopreservation. Cryoprotectants used in this study were methanol 10% and skim milk in various concentrations (5%; 10%; 15%; 20%; and 25%). The ratio of sperm and diluent solution in this study was 1:9. Extenders used are fish Ringer's solution. Sperm is stored in the deep freezer at a temperature of –34 ^oC for 48 hours. Fresh sperm is evaluated first to test the sperm eligibility for cryopreservation. Fresh sperm is evaluated macroscopically (color, sperm volume and pH), microscopically (motility, viability and abnormality), and the ability of fertilization by calculating the ability of fertilization. Post-cryopreservation sperm was evaluated microscopically and fertilization ability. The results showed that 10% methanol cryoprotectant and various concentrations of skim milk had a significant effect on several concentrations (P<0.05) on motility, viability, abnormality and fertilization ability. Based on the ANOVA one-way statistical test followed by the Tukey test. Optimum cryoprotectant of methanol 10% and skim milk 10% are the best concentrations that produce motility of 82.90 ± 1.40%; viability 79.00 ± 2.16%; the lowest abnormality was 27.75 ± 1.26% and the fertilization ability was 91.25 ± 2.21%. "

"Ikan kancra Tor soromerupakan ikan air tawar yang digemari oleh masyarakat untuk dikonsumsi dan digunakan untuk upacara adat. Sehingga permintaan konsumen terhadap ikan kancra meningkat. Hal tersebut menyebabkan terjadinya penangkapan ikan kancra yang berlebih di alam sehingga populasi ikan kancra menurun. Oleh karena itu, perlu dilakukan pelestarian ikan kancra dengan cara kriopreservasi spermatozoa. Penelitian menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan dan enam ulangan. Empat perlakuan terdiri atas sari buah tomat 0% + DMSO 10% (SBt 0%); sari buah tomat 10% + DMSO 10% (SBt 10%); sari buah tomat 20% + DMSO 10% (SBt 20%); dan sari buah tomat 30% + DMSO 10% (SBt 30%). Tujuan dari penelitian ini adalah untuk mengetahui pemberian sari buah tomat Lycopersicon esculentumsebagai antioksidan alami terhadap spermatozoa ikan kancra 24 jam pascakriopreservasi. Parameter uji kualitas spermatozoa meliputi motilitas, viabilitas, abnormalitas, dan kemampuan fertilisasi. Data hasil penelitian yang diperoleh diuji dengan menggunakan uji Analisis Variansi (ANAVA) satu faktor. Hasil penelitian menunjukkan adanya perbedaan nyata (P < 0,05) pada nilai rata-rata persentase viabilitas dan kemampuan fertilisasi serta tidak adanya perbedaan nyata (P > 0,05) pada nilai rata-rata persentase motilitas dan abnormalitas. Hasil penelitian membuktikan bahwa penambahan konsentrasi 10% sari buah tomat dalam ekstender memberikan pengaruh yang cukup positif terhadap kualitas spermatozoa ikan kancra 24 jam pascakriopreservasi, yaitu dengan ditunjukkan nilai rerata persentase motilitas (32,57 ± 5,94%); viabilitas (14,5 ± 4,88%); abnormalitas (74,16 ± 2,13%); dan kemampuan fertilisasi (81,25 ± 6,07%).  Kancra fish Tor soro are freshwater fish that are favored by the community for consumption and use for traditional ceremonies. So that consumer demand for kancra fish increases. This causes the occurrence of over-fishing in the wild which causes the population of fish to decrease. Therefore, it is necessary to preserve kancra fish by cryopreservation of spermatozoa. The study used a completely randomized design with four treatments and six replications. Four treatments consisted of 0% tomato juice + 10% DMSO (0% SBt); 10% tomato juice + 10% DMSO (10% SBt); 20% tomato juice + 10% DMSO (20% SBt); and 30% tomato juice + 10% DMSO (SBt 30%). The purpose of this study was to determine the administration of tomato juice Lycopersicon esculentum as a natural antioxidant on spermatozoa of kancra fish 24 hours post cryopreservation. Spermatozoa quality test parameters include motility, viability, abnormality, and fertilization rates. The research data obtained were tested using the one-factor Variance Analysis (ANOVA) test. The results showed a significant difference (P < 0.05) in the average value of the percentage of viability and fertilization rates, and no significant difference (P > 0.05) in the average value of the percentage of motility and abnormality. The results of the study prove that the addition of 10% concentration of tomato juice in the extender has a quite positive influence on the quality of spermatozoa of kancra fish 24 hours post cryopreservation, that is indicated by the mean value of the percentage of motility (32.57 ± 5.94%); viability (14.5 ± 4.88%); abnormalities (74.16 ± 2.13%); and fertilization ability (81.25 ± 6.07%)."

"Penelitian kriopreservasi spermatozoa ikan kerapu kertang memiliki tujuan mengetahui pengaruh berbagai konsentrasi susu skim (0%, 5%, 10%, 15%, 20%, 25%) yang dikombinasikan dengan gliserol 6% terhadap motilitas, viabilitas, dan abnormalitas serta kemampuan fertilisasi spermatozoa ikan kerapu kertang pascakriopreservasi terhadap sel telur ikan kerapu macan. Larutan pengencer yang digunakan dalam penelitian adalah larutan marine fish Ringer, gliserol 6%, susu skim berbagai konsentrasi. Rasio pengenceran yang digunakan adalah 1:9. Kriopreservasi dilakukan dalam freezer pada suhu -20°C, dengan lama penyimpanan selama 48 jam. Spermatozoa hasil kriopreservasi selama 48 jam digunakan untuk membuahi sel telur ikan kerapu macan. Hasil fertilisasi digunakan untuk mengukur parameter kualitas spermatozoa yang baik. Hasil uji ANAVA satu arah menunjukkan pemberian berbagai konsentrasi susu skim memiliki nilai rata-rata persentase motilitas, viabilitas, dan abnormalitas spermatozoa ikan kerapu kertang 48 jam pascakriopreservasi yang berbeda nyata (P˃0,05). Hasil terbaik ditunjukkan pada konsentrasi susu skim 20% dengan nilai persentase motilitas, viabilitas, dan abnormalitas secara berurutan sebesar 80,51 ± 3,46%; 81,24 ± 2,34%; dan 25,35 ± 2,04 %. Hasil analisis pada fertilisasi spermatozoa pascakriopreservasi menyatakan bahwa nilai rata-rata persentase fertilisasi tidak berbeda nyata antar perlakuan, namun pada konsentrasi susu skim 20% memberikan kemampuan fertilisasi yang baik yaitu 68 ± 1,70%.

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The objective of this study was to discover the effect of various concentration skim milk from (0%, 5%, 10%, 15%, 20%, and 25%) and combined with glycerol 6% which give the best effect towards motility, viability, abnormality and fertilization for Ephinephelus fuscogutattus (Forsskal 1775) capability of Ephinephelus lanceolatus (Bloch 1970) spermatozoa 48 hour after freezing. We used marine fish Ringer, glycerol 6%, and various concentration skim milk. Dilute the spermatozoa at 1 : 9 ratio. Cryopreservation is carried out in a freezer with a temperature of -20°C with a storage time of 48 hours. Cryopreservation spermatozoa for 48 hours is used to fertilize the egg Ephinephelus fuscogutattus (Forsskal 1775). Fertilization results are used to measure the quality parameter of spermatozoa Ephinephelus lanceolatus (Bloch 1970). Based on the ANAVA analysis, the treatment groups showed significant difference in average motility, viability and spermatozoa abnormality percentage with the control (P˃0.05). ANAVA analysis showed best result are obtained from 20% skim milk with average motility (80.51 ± 3.46%), sperm viability (81.24 ± 2.34%), and sperm abnormality (25.35 ± 2.04%). The results of analysis on fertilization with sperm post- cryopreservation stated that the average value of the percentage of fertilization was not significantly different between treatments, but at the concentration of skim milk 20% give a best ability of fertilization which was 68 ± 1.70%."

"ABSTRAKPenelitian kombinasi konsentrasi kuning telur sebagai krioprotektan belum pernahdilakukan pada ikan Botia (Chromobotia macrachanthus). Penelitian menggunakankonsentrasi kuning telur sebagai krioprotektan ekstraseluler dan metanol 10% sebagaikrioprotektan intraseluler. Konsentrasi kuning telur (0%, 5%, 7%, 9%, 11%, 13%, 15%,17%) dan penggunaan Carboxymethyl Cellulose (CMC) 1% terhadap motilitas,viabilitas dan abnormalitas spermatozoa ikan botia 24 jam pascakriopreservasi.Preservasi dilakukan pada tabung nitrogen cair dengan suhu -196°C. Berdasarkan hasiluji ANAVA satu arah menunjukkan pemberian berbagai konsentrasi kuning telurberpengaruh nyata (p<0,05) terhadap persentase motilitas, viabilitas, dan abnormalitasspermatozoa ikan Botia 24 jam pascakriopreservasi. Konsentrasi kuning telur optimumialah 15%, dengan nilai persentase motilitas (96,43 ± 1,49%), nilai persentase viabilitas(84,25 ± 1,26%) serta nilai persentase abnormalitas terendah (11,50 ± 1,29%). UjiTukey persentase nilai fertilitas telur 24 jam pascakriopreservasi tertinggi padakonsentrasi kuning telur 15% (50,64 ± 4,37%).

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ABSTRACTThe combination effect of egg yolk and 10% methanol on Botia fish spermatozoaquality and has not been performed, yet. Accordingly, the objrctive of study was: first,to evaluate the motility rate, viability rate, and abnormality of Botia fish spermatozoa 1day after cryopreservation. Second, to evaluate the fertility rate of Botia fish egg afterfertilized by cryopreserved sperm. The various concentration of egg yolk used were,5%, 7%, 9%, 11%, 13%, 15%, and 17% whereas the negative control (0%) used 10%methanol only without egg yolk. While, the positive of control used 1% of CMC. Botiasperm and egg were collected by hand stripping method. Physical and chemical ofbotia sperm had been observed by visual observation whereas the otility rate, vuiabilityrate, abnormality rate and fertility rate determined by light microscope. Botia fishsperm were mixed with cryoprotectand and extender before freezing at -196℃ (in LN).cryopreservation of botia fish sperm were conducted for one day. Based on one-wayANOVA test, gave the significant different between treatment group and control.Furthermore, according to Tuket test, they were gave the significant different (P<0.05)also among treatment group. Fifteen percent of egg yolk was optimum concentreationthat gave the highest motility rate, (96.43 ± 1.49%), and the highest viability (84.25 ±1.26%) and showed the lowest percentage of abnormality (11.50 ± 1.29%), and also thehighest fertility rate of Botia fish egg that (50,64 ± 4,37%) with protected by 15% ofegg yolk."