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"Latar Belakang : Penambahan fluor ke dalam proses PILP memiliki potensi meningkatkan remineralisasi intrafibril dan ekstrafibril kolagen dentin. Tujuan : Penelitian ini bertujuan untuk mengetahui pengaruh penambahan fluor dalam proses PILP terhadap remineralisasi intrafibril dan ekstrafibril kolagen dentin. Metode : 8 sampel blok dentin dibagi ke dalam 4 kelompok: Kelompok 1 demineralized dentin sebagai kontrol, kelompok 2 demineralized dentin disimpan dalam larutan remineralisasi PILP yang ditambahkan fluor 5 ppm, kelompok 3 demineralized dentin disimpan dalam larutan remineralisasi PILP yang ditambahkan fluor 25 ppm, kelompok 4 demineralized dentin disimpan dalam larutan remineralisasi PILP tanpa fluor (0 ppm) Kemudian seluruh sampel disimpan dalam shaking incubator pada suhu 37°C selama 14 hari. Selanjutnya dilakukan pemeriksaan dengan TEM/SAED untuk melihat remineralisasi intrafibril dan ekstrafibril kolagen dentin dan kristalinitas kristal yang dihasilkan. Hasil : Terjadi remineralisasi intrafibril dan ekstrafibril kolagen dentin pada kelompok perlakuan 2, 3, dan 4. Remineralisasi intrafibril dan ekstrafibril terlihat paling padat pada kelompok 2. Terdapat perbedaan kristalinitas kristal di antara kelompok 1, 2, 3, dan 4. Kesimpulan: Penambahan fluor 5 ppm ke dalam proses PILP menghasilkan remineralisasi intrafibril dan ekstrafibril kolagen dentin. Remineralisasi yang dihasilkan berupa kristal apatit dengan tingkat kristalinitas yang padat.

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Background: Fluoride addition into PILP process has the potential to increase intrafibrillar and extrafibrillar dentin collagen remineralization. Objective: This research aim to evaluate the influence of fluoride addition into PILP process towards intrafibrillar and extrafibrillar dentin collagen remineralization. Methods: 8 dentin blocks were divided into 4 sample groups. Group 1 (demineralized dentin) as control group, group 2 is demineralized dentin that were soaked in PILP process with 5 ppm fluoride addition, group 3 is demineralized dentin that were soaked in PILP process with 25 ppm fluoride addition, and group 4 is demineralized dentin that were soaked in PILP process with no fluor (0 ppm) addition. All of the samples were incubated in shaking incubator at 37o C for 14 days. Result: Intrafibrillar and extrafibrillar dentin collagen remineralization occurred in group 2, 3, and 4. The most dense intrafibrillar and extrafibrillar dentin collagen remineralization was seen in group 2. There are differences of crystal's crystallinity between group 1, 2, 3, and 4. Conclusion: 5 ppm fluoride addition into PILP process produced intrafibrillar and extrafibrillar dentin collagen remineralization. The remineralization were consisted by high-density apatite crystals."

"Latar belakang: Silver Diamine Fluoride dan Laser CO2 merupakan alternatif perawatan yang dapat dipilih untuk menangani hipersensivitas denton.Tujuan: Mengetahui efektivitas penggunaan Silver Diamine Fluoride dan Laser CO2 dalam menurunkan skor hipersensitivitas dentin. Metode: Penelitian ini menggunakan desain single blind, split-mouth randomized controlled trial yang melibatkan 16 subjek dengan keluhan gigi sensitif dan memiliki minimal dua gigi yang positif terhadap uji evaporatif atau uji thermal. Subjek akan diaplikasikan Silver Diamine Fluoride dan Laser CO2. Pengukuran hipersensitivitas dentin dilakukan dengan stimulus evaporatif dan termal dan DIAGNOdent pasca aplikasi, 7 hari dan 14 hari setelah aplikasi. Hasil: Terjadi penurunan skor Hipersensitivitas dan DIAGNOdent yang berbeda bermakna terhadap data baseline segera pasca aplikasi, 7 hari dan 14 hari pasca aplikasi p=0,000 untuk stimulus evaporatif, termal dan DIAGNOdent . Tetapi tidak terdapat perbedaan bermakna secara statistik antara aplikasi Silver Diamine Fluoride dan aplikasi Silver Diamine Fluoride dan Laser CO2 pada setiap pengukuran. Kesimpulan: Penggunaan Silver Diamine Fluoride dan Laser CO2 efektif dalam menurunkan skor hipersensitivitas dentin.

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Background: Silver Diamine fluoride and CO2 Laser are a new alternative treatment for dentin hypersensitivity.

Objective: To evaluate the efficacy of Silver Diamine Fluoride and CO2 Laser in reducing dentin hypersensitivity score.

Methods: The study used a single blind, split mouth design randomized controlled trial involving 16 subjects with symptoms of sensitive teeth and have at least two hypersensitive teeth which are positive to evaporative or thermal test. The subject teeth surface will be applied Silver diamine fluoride and CO2 Laser will be applied to the subject teeth surface.

Results: This clinical study demonstrated that the application of Silver Diamine Fluoride and CO2 Laser reduce dentin hypersensitivity significantly in each measurement p 0,000 for evaporative and thermal stimulus. But there is no statistically significant differences between the two application.

Conclusion: The usage of Silver Diamine Fluoride and CO2 Laser are effective to reduce the degree of dentin hypersensitivity. "

"Latar Belakang: Proses PILP adalah proses remineralisasi yang menggunakan makromolekul bermuatan, yakni polyaspartic acid, untuk menstabilkan ion calcium phosphate dalam larutan tersaturasi, dan mencegah nukleasi spontan serta presipitasi mineral. Penambahan ion fluoride pada proses ini akan membentuk kristal fluorapatite yang memiiki kekerasan lebih tinggi dari hidroksiapatit. Tujuan:Penelitian ini bertujuan untuk mengetahu pengaruh penambahan fluoride dalam larutan remineralisasi melalui proses PILP terhadap kekerasan email. Metode: 25 sampel gigi dibagi menjadi 5 kelompok. Kelompok 1 (email normal) sebagai kontrol positif, kelompok 2 (email terdemineralisasi) sebagai kontrol negatif, kelompok 3 email yang diremineralisasi melalui proses PILP, kelompok 4 email yang diremineralisasi melalui proses PILP dengan penambahan fluoride 5 ppm, kelompok 5 email yang diremineralisasi melalui proses PILP dengan penambahan fluoride 25 ppm. Seluruh sampel disimpan dalam inkubator pada suhu 370C selama 14 hari. Selanjutnya dilakukan uji kekerasan mikro untuk melihat kekerasan email. Data yang diperoleh dianalisis secara statistik dengan one-way ANOVA dan uji Post Hoc Tamhane. Hasil: Terdapat perbedaan bermakna kekerasan mikro email setelah diremineralisasi melalui proses PILP tanpa dan dengan penambahan fluoride 5 ppm dan 25 ppm. Kesimpulan:Remineralisasi melalui proses PILP dengan penambhan fluoride 5 ppm memiliki kekerasan mikro yang lebih tinggi dibandingkan tanpa penambahan fluoride dan dengan penambahan fluoride 25 ppm.

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Background: PILP process is a remimineralization used charged macromolecule, polyaspartic acid, to stabilize calcium and phophate ion in order to prevent mineral spontaneus nucleation and precipitation. Fluoride was addedto form fluorapatite crystals which have a higer microhardness than hydroxyapatite. Objective: To determine the effect of adding fluoride in remineralization solution through the PILP process on enamel microhardness. Methods: 25 teeth were divided into 5 groups. Group 1 (normal enamel) as a positive control, group 2 (demineralized enamel) as a negative control, group 3 demineralized enamel which remineralized through PILPprocess, group 4 demineralized enamel which remineralized through PILP process with 5 ppm fluoride, group 5 demineralized enamel which remineralized through PILP process with 25 ppm fluoride. All samples were stored in an incubator at 370C for 14 days. Vicker's microhardness test was performed to see enamel microhardness. The data were statistically analyzed with one-way ANOVA and Tamhane Post Hoc Test. Result: There is a difference between enamel microhardness after being remineralized through PILP process without and with 5 and 25 ppm fluoride.Conclusion: Remineralization via PILP process with addition of 5 ppm fluoride has a higher microhardness value than without and with 25 ppm fluoride."

"Latar Belakang: Remineralisasi pada dentin affected dapat terjadi secara guided tissue remineralization (GTR). Remineralisasi ini terjadi pada matriks intrafibrilar kolagen dentin karena peran protein non kolagen yaitu Dentin Matriks Protein 1 (DMP1) yang dapat rusak saat proses demineralisasi. Dibutuhkan material analog pengganti DMP1 untuk proses remineralisasi, salah satunya adalah asam poliaspartik dalam proses Polymer-Induced Liquid Precursor (PILP). Tujuan: Menganalisis remineralisasi yang terjadi pada demineralized dentin setelah diinduksi oleh asam poliaspartik dalam proses PILP. Metode: Sampel berupa dentin blok direndam pada larutan demineralisasi lalu dibagi menjadi empat kelompok, yaitu kelompok demineralized dentin tanpa perendaman larutan asam poliaspartik dan kelompok dengan perendaman larutan asam poliaspastik selama 3, 7, dan 14 hari. Sampel di evaluasi dengan uji SEM dan EDX. Hasil: Terdapat perbedaan signifikan antara kelompok demineralized dentin dengan kelompok remineralisasi asam poliaspartik pada hari ke 3, 7, dan 14. Remineralisasi yang terjadi berupa deposit ion kalsium dan fosfat. Kesimpulan: Asam poliaspartik dalam proses PILP memiliki potensi untuk meremineralisasi demineralized dentin.

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Background: Remineralization on affected dentin can be occurred by guided tissue remineralization (GTR) method. The remineralization process took place in intrafibilar matrix dentin collagen which regulated by a non collagenous protein, Dentin Matrix Protein (DMP 1) which can be destroyed during demineralization process. Remineralization process requires non collagenous protein analog material, one in particular is poliaspartic acid in Polymer-Induced Liquid Precursor (PILP) process. Objective: To analyze remineralization process that occured on demineralized dentin after application polyaspartic acid in PILP process. Method: Dentin block sample was soaked in demineralized solution. The sample then divided into four groups which are demineralized dentin without application of poliasparticacid solution, and demineralized dentin soaked in polyaspartic acid solution in the period of 3 ,7 and 14 days. The samples were evaluated by using SEM and EDX. Result: A statistically significant result between demineralized dentin group and remineralization with poliaspartic acid group within 3, 7, 14 days. Remineralization occurred by calcium and phosphate ions deposition. Conclusion: Polyaspartic acid in PILP process has the capability of remineralizing demineralized dentin."

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Latar Belakang: Perawatan karies dengan minimal intervensi, yaitu dengan membuang infected dentin, dan meninggalkan affected dentin, kemudian dilakukan remineralisasi pada affected dentin. Pada affected dentin masih terdapat ikatan silang kolagen. Remineralisasi dentin lebih kompleks karena pada karies dentin sudah tidak ada sisa kristal mineral. Untuk terjadinya remineralisasi ekstrafibrilar dan intrafibrilar pada dentin, maka dibutuhkan dentin matrix protein 1 (DMP 1)  yang merupakan protein non-kolagen. Proses polymer-induced liquid precursor (PILP) merupakan metode Guided Tissue Remineralization yang bertujuan untuk remineralisasi dentin secara intrafibrilar dan ekstrafibrilar dengan penambahan polimer sebagai analog DMP1. Salah satu material analog protein non kolagen adalah asam poliaspartik.

Tujuan: Mengevaluasi remineralisasi dentin pada permukaan demineralized dentin setelah perendaman dengan larutan remineralisasi yang mengandung asam poliaspartik sebagai analog protein non kolagen.

Metode: Empat kelompok dilakukan demineralisasi buatan. Setelah itu, tiga kelompok dilakukan perendaman dengan larutan remineralisasi yang mengandung asam poliaspartik, sedaangkan satu kelompok tidak dilakukan perendaman larutan remineralisasi asam poliaspartik. Evaluasi remineralisasi dengan Micro-CT.

Hasil: Terlihat remineralisasi pada permukaan demineralized dentin yang ditandai dengan penurunan kedalaman lesi demineralized dentin yang ditandai dengan peningkatan grey scale index setelah dilakukan perendaman dengan larutan remineralisasi asam poliaspartik. Perbandingan rerata empat kelompok menunjukkan perbedaan bermakna.

Kesimpulan: Asam poliaspartik memiliki potensi untuk meremineralisasi demineralized dentin.

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Background: Caries treatment with minimal intervention, namely by removing infected dentine, and leaving affected dentine, then remineralization of affected dentine. In the affected dentine there are still have collagen cross bonds. Dentin remineralization is more complex because there is no residual mineral crystals in dentinal caries. For extrafibrillary and intrafibrillary remineralization in dentine, dentin matrix protein 1 (DMP 1) is a non-collagen protein. The polymer-induced liquid precursor (PILP) process is a Guided Tissue Remineralization method which aims to remineralize intrafibrilar and extafibrilar dentine by adding polymers as analogous to DMP1. One of the non collagen protein analog material is polyacpartic acid.

Objective: To evaluate remineralization of dentine on the demineralized dentin surface after immersion with remineralization solutions containing polyacpartic acid as a non-collagen protein analog.

Method: Four groups carried out artificial demineralization. After that, three groups were immersed with remineralization solution containing polypartic acid, while one group was not immersed in polyacpartic acid remineralization solution. Remineralization evaluation with Micro-CT.

Results: It appears remineralization on the demineralized dentin surface which is characterized by a decrease in the depth of demineralized dentin lesions which is characterized by an increase in gray scale index after immersion with polyacpartic acid remineralization solution. The comparison of the mean of the four groups showed significant differences.

Conclusion: Polyacpartic acid has the potential to remineralize for demineralized dentine.

 

 

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"Penelitian ini membuktikan efektifitas teri jengki (Stolephorus insularis) sebagai fluoridasi gigi dengan acuan kedalaman intrusi fluor. Digunakan metode eksperimental laboratorik in vivo. Subjek 14 ekor tikus Sprague dawley dibagi menjadi kelompok baseline, kontrol negatif pakan, kontrol negatif oles, metode pakan teri, dan metode oles larutan teri. Setelah perlakuan 15 hari, gigi dipotong transversal, diproses untuk uji intrusi fluor menggunakan mikroskop fluoresensi. Didapatkan hasil peningkatan intrusi fluor pada kelompok eksperimental dibandingkan kontrol negatif (p<0,05). Intrusi fluor metode oles lebih tinggi dibandingkan metode pakan (p <0,05). Jadi, aplikasi teri jengki, baik lewat pengunyahan maupun pengolesan, meningkatkan intrusi fluor pada email.

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The effectiveness of anchovy (Stolephorus insularis) as a fluoridative agent is measured by depth of fluoride intrusion. This study used experimental laboratory method. 14 Sprague Dawley rats were divided into groups of baseline, experimental (feeding and smearing), and their negative controls. After 15 days, teeth were cut transversely and fluoride intrusions were observed using fluorescence microscope. There were increased fluoride intrusion in enamel of experimental groups compared to their negative controls (p<0.05). Fluoride intrusion of smearing group is higher than feeding group (p <0.05). Thus, application of anchovy substrate, either by chewing or smearing, increases fluoride intrusion in tooth enamel."

"Penelitian bertujuan mengetahui pengaruh besar tekanan dan lama scrubbing terhadap kuat rekat geser self adhering flowable composite pada dentin. Self adhering flowable composite diaplikasikan dengan tekanan scrubbing 1, 2, dan 3 gram masing-masing selama 15, 20, dan 25 detik. Resin komposit diaplikasikan secara inkremental. Polimerisasi dengan sinar selama 20 detik. Kuat rekat geser diuji menggunakan Testing Machine dan dianalisa dengan ANOVA diikuti Post Hoc Test Bonferroni. Daerah patahan diobservasi menggunakan SEM. Kuat rekat geser tertinggi pada tekanan scrubbing 3 gram selama 25 detik. Perbedaan besar tekanan dan waktu saat scrubbing mempengaruhi kuat rekat geser self adhering flowable composite pada dentin.

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Effect of different scrubbing pressure and duration on shear bond strength of self adhering flowable composite to dentin was evaluated. Self adhering flowable composite was applied on dentin with 1, 2, and 3 grams pressure for 15, 20, and 25 seconds. Composite was constructed incrementally. Polymerization for 20 seconds. Shear bond strength was tested with a Testing Machine and analyzed by ANOVA and Bonferroni. Fractured area was observed with a SEM. The highest shear bond strength was obtained in 3 grams scrubbing pressure for 25 seconds. The different scrubbing pressure and duration influenced shear bond strength of self adhering flowable composite to dentin."

"ABSTRAKLatar belakang: Carboximethyl Chitosan / Amorphous Calcium Phosphate (CMC/ACP) sebagai material analog non-protein mempunyai kemampuan meremineralisasi dentin. Gypsum sebagai bahan pencampur yang dapat memudahkan aplikasi. Tujuan penelitian ini adalah melihat pengaruh penambahan gypsum pada material analog non-protein CMC/ACP. Metode: 27 kavitas dibagi menjadi 3 kelompok. Kelompok 1 dentin demineralisasi tidak diaplikasi bahan, kelompok 2 dentin demineralisasi diaplikasi CMC/ACP, kelompok 3 dentin demineralisasi diaplikasi gypsum+CMC/ACP. Diperiksa pada hari ke-14 menggunakan SEM-EDX. Hasil: gypsum tidak memengaruhi kemampuan material analog non-protein CMC/ACP dalam remineralisasi dentin.

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ABSTRACTBackground: Carboximethyl Chitosan / Amorphous Calcium Phosphate (CMC/ACP) is analog material non-protein that have dentine remineralization ability.  While Gypsum is mixing material that can facilitate the application. Objective of this study was to see the effect of gypsum addition on analog material non-protein CMC/ACP. Methods: 27 cavities were divided into 3 groups. Group 1 were dentine demineralization without any material applied. Group 2 were dentine demineralization with CMC/ACP material applied, and group 3 were dentine demineralization with gypsum + CMC/ACP material applied. Checked on day 14 using SEM-EDX. Result:  Gypsum was not affect material ability of analog non-protein CMC/ACP in dentine remineralization.

 

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"Latar Belakang: Saat ini perawatan pulpa vital mengacu pada konsep minimal intervensi, yaitu dengan meninggalkan lapisan affected dentin saat ekskavasi karena masih terdapat ikatan silang kolagen sehingga dapat dilakukan upaya remineralisasi. Remineralisasi melalui aplikasi material bioaktif Mineral Trioxide Aggregate (MTA) belum dapat menghasilkan karakteristik dentin menyerupai dentin normal karena pembentukan mineral hanya terjadi pada ruang ekstrafibrillar. Penggunaan material analog protein non kolagen dibutuhkan untuk menstabilkan nano Amorphous Calcium Phosphate (ACP) agar dapat memasuki ruang intrafibrillar, salah satunya menggunakan Carboxymethyl Chitosan (CMC).Tujuan: mengevaluasi remineralisasi dentin pada permukaan dentin terdemineralisasi setelah aplikasi material MTA dan material modifikasi MTA-CMC dengan melihat morfologi permukaan dan komposisi ion pada dentin.Metode: remineralisasi dilakukan melalui aplikasi material MTA, MTA-CMC 5% dan 10% selama 14 hari pada permukaan sampel dentin yang terdemineralisasi. Pengamatan morfologi dilakukan dengan Scanning Electron Microscope (SEM) dan konfirmasi komposisi ion dengan Energy Dispersive X-ray Spectroscopy (EDX).Hasil: terdapat perbedaan morfologi pada permukaan dentin setelah dilakukan remineralisasi melalui aplikasi MTA, MTA-CMC 5%, dan MTA-CMC 10% selama 14 hari yang dievaluasi menggunakan SEM. Hasil analisis EDX mengonfirmasi adanya peningkatan kandungan kalsium dan fosfor pada permukaan dentin yang diremineralisasi dengan material MTA-CMC.Kesimpulan: remineralisasi melalui aplikasi material modifikasi MTA-CMC dapat memengaruhi morfologi permukaan dan komposisi ion pada permukaan dentin yang terdemineralisasi.

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Background: Vital pulp treatment refers to the concept of minimal intervention by preserving the affected dentin layer in the excavation process because there are still collagen cross-linked to induce remineralization. Remineralization through the application of bioactive material Mineral Trioxide Aggregate (MTA) produce different dentin characteristics to normal dentin because mineral formation only occurs in the extrafibrillar space. The use of non-collagen protein analog materials is needed to stabilize the nano Amorphous Calcium Phosphate (ACP) to get into the intrafibrillar space, one of which is Carboxymethyl Chitosan (CMC).

Objective: To evaluate dentin remineralization on the surface of demineralized dentin after the application of MTA and MTA-CMC modified material by looking at the surface morphology and ionic composition of the dentin.

Methods: The remineralization was carried out through the application of MTA,MTA-CMC 5%, MTA-CMC 10% materials for 14 days on the surface of demineralized dentin samples. Morphological observations were carried out by Scanning Electron Microscope (SEM) and confirmation of ion composition by Energy Dispersive X-ray Spectroscopy (EDX). Results: there were morphological differences on the dentin surface after remineralization through the application of MTA, MTA-CMC 5%, and MTA-CMC 10% for 14 days which were evaluated using SEM. The results of the EDX analysis confirmed an increase in the content of calcium and phosphorus on the dentin surface remineralized with MTA-CMC material.

Conclusion: remineralization through the application of MTA-CMC modified material can affect the surface morphology and ion composition on the demineralized dentin surface."

"Latar belakang: Pada lesi karies dalam sisa ketebalan dentin yang masih tersisa sangat tipis sehingga pembuangan seluruh infected dentin beresiko besar terhadap terbukanya pulpa. Teknik minimal invasif diperlukan, yaitu partial caries excavation dengan metode pembuangan jaringan infected dentin sebagian dan penggunaan material bioaktif MTA yang dapat memicu terjadinya remineralisasi. Tujuan: Untuk membandingkan remineralisasi pada affected dentin lesi karies dalam dengan pembuangan seluruh dan sebagian infected dentin setelah aplikasi MTA. Metode: Subjek dibagi dua kelompok, kelompok I dilakukan pembuangan sebagian infected dentin dan diaplikasikan MTA, kelompok II dilakukan pembuangan seluruh infected dentin dan diaplikasikan MTA. Masing-masing kelompok diukur pixel gray value sebelum dan 4 minggu setelah dilakukan aplikasi MTA, lalu dibandingkan. Selain itu dibandingkan peningkatan gray value pada kedua kelompok tersebut. Hasil: Terjadi remineralisasi affected dentin kelompok I dan II setelah aplikasi MTA. Tidak terdapat perbedaan bermakna tingkat remineralisasi affected dentin pada kelompok 1 dan 2 setelah aplikasi MTA selama 4 minggu. Kesimpulan: Terjadi remineralisasi affected dentin pada kedua kelompok baik dengan pembuangan sebagian maupun seluruh infected dentin lesi karies dalam.

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Background Deep carious lesion that only thin dentin remains in the remaining dentin thickness that caused a high risk to the pulp exposure in the removal of all infected dentin. Minimally invasive techniques are required, which are a partial caries excavation method in infected dentin tissue and the use of bioactive material that can promote MTA remineralization. Aim to compare the remineralization of deep carious lesion affected dentin with the removal in some parts and all of the infected dentin after the application of the MTA. Methods Subjects are divided into two groups, in which group I is getting the removal in only some parts of the infected dentin and the application of the MTA, group II is getting the removal in all of the infected dentin and the MTA application. Each group is measured on the pixel grey value before and four weeks after the application of the MTA, and then compare. Moreover, compare the enhancement of the grey value of those groups. Result Reminalisation is occurred in both of the groups after the application of the MTA. there is no significance difference in the reminalization level of the affected dentin in both group I and group II after the application of the MTA during four weeks. Conclusion Reminalization is occurred in the affected dentin in both of the groups either by only removal in some part or all of the carious lesion infected dentin."