Hasil Pencarian  ::  Simpan CSV :: Kembali

"

---

ABSTRACTOriginally published in 1938, this book presents a detailed examination of synthetic embryology. Intended neither as an introductory guide nor a systematic treatise, the text presents the most significant material regarding the ontogenetic problem as matters stood at the time of publication. Illustrative figures and a bibliographical index are also included. This book will be of value to anyone with an interest in the development of embryology and the history of science. "

"Salah satu jenis ikan hias yang menarik adalah ikan neon tetra Paracheirodon innesi, Myers 1936; yang merupakan ikan asli yang beraal dari Sungai Amazon, Amerika Selatan. Ikan neon tetra sangat sulit dibudidayakan. Oleh karena itu informasi tentang embriogenesis dan perkembangan stadia awal pada ikan neon tetra sangat diperlukan untuk mendukung keberhasilan pengembangbiakan. Penelitian ini bertujuan untuk mengetahui embriogenesis dan perkembangan larva ikan Paracheirodon innesi. Telur yang digunakan berupa hasil pemijahan alami di Balai Budidaya Riset Ikan Hias, Depok. Pengamatan embriologi dimulai pada saat ikan memijah sampai telur ikan menetas, sedangkan perkembangan larva dimulai dari larva menetas sampai menjadi benih atau perkembangan sudah sempurna. Pengamatan dilakukan setiap hari dibawah mikroskop binokuler olympus XZS10 perbesaran 8-25 kali. Hasil penelitian menunjukkan bahwa perkembangan embrio telur terjadi selama satu hari atau 24 jam hingga menetas menjadi larva pada suhu 24-26oC. Proses perkembangan embrio dimulai dari fase morula, blastula, gastrula.

---

One of the interesting ornamental fish species is the neon tetra Paracheirodon innesi, Myers 1936; which is a native fish originating from the Amazon River, South America. Neon tetra fish are very difficult to cultivate. Therefore, the domestication the fish species is the first important step toward developing the aquaqulture technology of the species which requires specific information on embryogenesis and the development of the early stadia P.Innesi. This study aimed to determine embryogenesis and larval depelopment of P.Innesi. The eggs and larvae used from the natural spawning of Paracheirodon innesi wild parents reared in the facility of the Balai Budidaya Riset Ikan Hias, Depok, Indonesia. Observation of embryogenesis started from eggs fertilization until hatching. The depelopment of larvae was observed from post hatching until fully developed as fish juvenile. Embryonic and larval development were monitored daily using an Olympus SZX10 binocular microscope with 8x-25x magnification. The result showed that the embryogenesis of P.Innesi lasted for one days or 24 hours until it hatched. The depelopment stages of the embryogenesis start from morula phase, blastula, gastrula."

"Genomic Control Process explores the biological phenomena around genomic regulatory systems that control and shape animal development processes, and which determine the nature of evolutionary processes that affect body plan. Unifying and simplifying the descriptions of development and evolution by focusing on the causality in these processes, it provides a comprehensive method of considering genomic control across diverse biological processes. This book is essential for graduate researchers in genomics, systems biology and molecular biology seeking to understand deep biological processes w."

"ABSTRAKPerubahan tingkat penanda epigenetik pada sperma memiliki dampak pada fertilisasi dan kontribusi sperma terhadap perkembangan embrio secara normal. Pada beberapa studi, perubahan metilasi pada DNA yang terdapat di dalam gen, memiliki kaitan dengan infertilitas dan keberhasilan teknologi reproduksi. Pemeriksaan Aniline Blue (AB) dan Toluidin Blue (TB) yang menganalisis maturitas dan kepadatan kromatin sperma ditentukan oleh ekspresi dari gen protamin. Namun sejauh ini, belum ada penelitian yang menganalisis tingkat maturitas dan kepadatan kromatin sperma sekaligus menilai status metilasi gen protamin 1 ini pada sperma pasien yang mengikuti program FIV dibandingkan dengan sperma pada laki-laki fertil normozoospermia dengan tingkat perkembangan zigot pada program FIV.Sampel penelitian ini berjumlah 60 orang, yang terdiri dari 30 sampel pasien yang mengikuti program FIV di Klinik Infertilitas Yasmin RSCM Kencana, dan 30 orang laki-laki fertil normozoospermia. Sampel ini diperiksa keadaan kromatin spermanya menggunakan pewarnaan AB dan TB, Data tingkat perkembangan zigot diperoleh dari data sekunder rekam medis Klinik Yasmin, kemudian analisis metilasi gen protamin 1 menggunakan MSP (Methylation Specific PCR) dan diukur pita yang terlihat secara semikuantitatif menggunakan ImageJ.Dari hasil penelitian diperoleh bahwa tingkat maturitas kromatin sperma pada ejakulat pasien FIV lebih rendah secara bermakna dibandingkan pada ejakulat laki-laki fertil normozoospermia. Maturitas kromatin sperma yang baik pada ejakulat pasien FIV memiliki arah korelasi positif yang tidak bermakna dengan tingkat perkembangan zigot, sedangkan pada tingkat kepadatan kromatin sebaliknya, dan memiliki korelasi bermakna pada kategori maturitas kromatin sperma yang tidak baik. Tingkat metilasi DNA gen protamin 1 pada pasien yang mengikuti program FIV lebih rendah bermakna dibandingkan dengan tingkat metilasi DNA gen protamin 1 pada ejakulat laki-laki fertil normozoospermia. Terdapat hubungan korelasi tidak bermakna dengan arah positif antara maturitas kromatin sperma dengan tingkat metilasi DNA gen protamin 1 namun pada kepadatan kromatin memiliki arah sebaliknya. Perubahan tingkat metilasi DNA pada gen Protamin 1 mempengaruhi perkembangan zigot pada program FIV namun tidak bermakna.

---

ABSTRACT

The changes of epigenetic marker level of sperm have an impact on fertilization and contribution sperm to the normally embryo development. In some studies, DNA methylation changes in genes, linked to infertility and the success of assisted reproductive technology. The assay of Aniline Blue (AB) and Toluidine Blue (TB) which analyzed maturity and integrity of sperm chromatin was determined by the expression of protamine gene. Up till now, there has not been any study to analyze the maturity level and integrity of sperm chromatin level, and methylation status of protamine 1 genes on the FIV patients sperm compared to sperm in normozoospermia fertile male with the level of zygote development in the IVF program.

Samples used in this study were ejaculate from patients who take IVF program at Yasmin Infertility Clinic, RSCM Kencana, and 30 normozoospermia fertile male as control. These samples were assayed the sperm chromatin using AB and TB staining. The data of zygote development level were obtained from secondary data of medical records of Yasmin Infertility Clinic, and the methylation level of protamine 1 gene were done MSP (methylation Specific PCR) and its band measured using ImageJ semi quantitatively.

The result showed that the level of sperm chromatin maturity of ejaculate IVF patients was significantly lower compared to normozoospermia fertile male. The maturity and chromatin integrity level of sperm in the ejaculate IVF patients with have weak correlation with the level of zygote development and significant in the bad category of sperm chromatin maturity. The DNA methylation level of protamine 1 gene in patients under when the IVF program was significantly lower compared to normozoospermia fertile male. There was not significantly with positif correlation between maturity chromatin of sperm with the DNA methylation of protamine 1 gene and the chromatin integrity was different. The changes of DNA methylation of Protamine 1 gene affects the level of zygote development in IVF program not significantly.Aniline Blue."