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Hasil Pencarian

Ditemukan 2354 dokumen yang sesuai dengan query
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Serdar Caskun
"the availabilty of assisted reproductive technologies and advances in molecular biology gave rise to preimplantation genetic diagnosis (PGD). PGD is an early form of prental diagnosis and determines the genotype of an embryo before implantation takes place to avoid the implantation of diseased embryos. this requires couples to adhere to a strict family planning and effective contraceptive strategy, and undergo in vitro fertilization (IVF) treatment. during IVF, sampling of the cells can be performed at different developmental stages from polar body biopsy to trophectoderm cells from the blastocysts. it is indicated in couples with a family history of monogenic autosomal or X-linked recessive or dominant disorders and in detecting chromosomal aberrations of the embryos. the genetic diagnosis is performed using appropriate molecular testing which might include polymerase chain reaction, fluorescent in situ hybridization, comparative genomic hybridization or microarrays. PGD is now a wellestablished procedure and offers an alternative reproductive choice for couples who are at risk of an affected child. in this review, the past, present and future aspect of PGD will be covered. "
Androcryogenics, 2010
176 JRSCB 1 (1) 2010
Artikel Jurnal  Universitas Indonesia Library
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Bobby Pambudi
"Latar Belakang : Neuronal ceroid lipofuscinoses (NCL) tipe 2 adalah kelompok penyakit langka yang diturunkan, bersifat autosomal resesif, dan progresif neurodegeneratif. Penyakit NCL tipe 2 disebabkan oleh mutasi pada gen TPP1 dengan prognosis buruk. Penyakit ini dapat diterapi dengan terapi sulih enzim yang mahal. Dengan melakukan carrier testing kita dapat melihat pembawa sifat dalam keluarga. Informasi tersebut berguna dalam mencegah keturunan berikut menjadi sakit dengan preimplantation genetic diagnosis (PGD). Salah satu teknik PGD adalah preimplantation genetic testing for monogenic disorder (PGT-M) dengan validasi analisis linkage dari short tandem repeat (STR). Tujuan penelitian ini untuk mendapatkan profil STR pada keluarga pasien NCL tipe 2 di Indonesia untuk persiapan PGD.
Metode : Penelitian ini merupakan suatu studi observasional analitik pada keluarga pasien dengan NCL tipe 2 yang sudah terkonfirmasi secara enzimatik dan genetik. Carrier testing dilakukan pada kedua orang tua dan saudara kandung subjek, dilanjutkan pencarian STR pada area  ± 1 Mb dari gen TPP1. Pada setiap kandidat STR dilakukan fragment analysis pada subjek, kedua orang tua dan saudara kandung, serta dilakukan linkage analysis untuk menilai penanda STR bersegregasi dalam keluarga dan dapat digunakan sebagai kandidat dalam PGT.
Hasil Penelitian : Terdapat 4 pasien dari 4 kota di Indonesia. Terdiri dari satu lelaki dan tiga perempuan. Keempat subjek menunjukkan gejala NCL tipe 2 klasik dengan awitan gejala usia 31-42 bulan. Ditemukan 3 varian patologis yaitu 1 varian frame-shift (c.583_584insTACA), 1 varian in-frame (c.1222_1224delAGT), dan 1 varian missense (c.679T>C). Hasil carrier testing menunjukkan semua orang tua sebagai carrier varian patogenik. Hasil fragment analysis dari dua buah STR D11S1996 dan D11S1338 menunjukkan segregasi dalam linkage analysis dalam keluarga subjek.
Kesimpulan : Pada keempat pasien NCL tipe 2 di Indonesia ditemukan 3 varian patogenik. Carrier testing menunjukkan semua orang tua adalah pembawa sifat. Kedua STR D11S1996 dan D11S1338 dapat dipakai dalam program PGD pada pasien NCL tipe 2 di Indonesia.

Background : Type 2 Neuronal Ceroid Lipofuscinoses (NCL) is a type of autosomal recessively inherited and progressive neurogenerative rare disease. This disease is due to TPP1 gene mutation, which have poor prognosis. Enzyme replacement therapy is available but with high cost. Carrier trait individual can be detected by carrier testing procedure, such as pre-implantation genetic diagnosis (PGD). These information can be useful to prevent disease occurence on subsequent generation. One PGD technique currently used is pre-implantation genetic testing for monogenic disorder (PGT-M) with linkage validation analysis of short tandem repeat (STR). The aim of this study was to search for STR marker in the type 2 NCL family in Indonesia for the preparation of PGD.
Methods : This is an analytical observational study on patient's family with enzymatic and genetically confirmed case of type 2 NCL. Carrier testing were done on both parents and sibling of case patients. STR sequence testing were done on ± 1 Mb area of TPP1 gene, with fragment analysis on each STR candidate. Linkage analysis were also performed to look for segregated STR pattern on the family, which can be used as a candidate in PGD.
Result : There were four case patients from 4 cities in Indonesia, 1 male and 3 female cases. These four cases manifested classic symptoms of type 2 NCL with onset age ranging from 31-42 months. Three pathological variants were found: 1 frame-shift variant (c.583_584insTACA), 1 in-frame variant (c.1222_1224delAGT), and 1 missense variant (c.679T>C). Carrier testing results showed all parents as pathogenic variant carrier. Fragment analysis from 2 STR (D11S1996 and D11S1338) showed segregation in linkage analysis on subject's family.
Conclusion : From 4 type 2 NCL cases in Indonesia, 3 pathogenic variants were found, with carrier testing showed both of their parents as carriers. Both D11S1996 and D11S1338 STR can be utilized as PGD diagnostic of type 2 NCL patients in Indonesia.
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Jakarta: Fakultas Kedokteran Universitas Indonesia, 2022
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UI - Tugas Akhir  Universitas Indonesia Library
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"the availabilty of assisted reproductive technologies and advances in molecular biology gave rise to preimplantation genetic diagnosis (PGD). PGD is an early form of prental diagnosis and determines the genotype of an embryo before implantation takes place to avoid the implantation of diseased embryos. this requires couples to adhere to a strict family planning and effective contraceptive strategy, and undergo in vitro fertilization (IVF) treatment. during IVF, sampling of the cells can be performed at different developmental stages from polar body biopsy to trophectoderm cells from the blastocysts. it is indicated in couples with a family history of monogenic autosomal or X-linked recessive or dominant disorders and in detecting chromosomal aberrations of the embryos. the genetic diagnosis is performed using appropriate molecular testing which might include polymerase chain reaction, fluorescent in situ hybridization, comparative genomic hybridization or microarrays. PGD is now a wellestablished procedure and offers an alternative reproductive choice for couples who are at risk of an affected child. in this review, the past, present and future aspect of PGD will be covered. "
JRSCB 1:1 (2010)
Artikel Jurnal  Universitas Indonesia Library
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Grariani Nufadianti
"Uveitis adalah inflamasi intraokular yang terjadi pada saluran uvea mata ataupun jaringan yang berada di dekatnya, diantaranya retina atau vitreous. Uveitis merupakan penyebab kebutaan nomor tiga di dunia dengan prevalensi tertinggi pada kelompok umur pekerja aktif 20-50 tahun . Selama ini diagnosis uveitis infeksi dan non-infeksi di Indonesia ditegakkan secara klinis dan didukung pemeriksaan serologi darah. Pemeriksaan antibodi pada cairan akuos dan serum serta marka genetik, khususnya HLA B-27 belum pernah dilakukan. Penelitian ini dilakukan untuk memberikan alternatif pendukung diagnosis klinis yaitu pemeriksaan deteksi molekuler HLA B-27 dan deteksi antibodi dengan perhitungan koefisien Goldmann-Witmer. Subjek penelitian adalah pasien uveitis aktif di Poliklinik Infeksi dan Imunologi RSCM Kirana yang berjumlah 79 orang. Berdasarkan koefisien Goldmann-Witmer, mikroba penyebab uveitis adalah Toxoplasma gondii dan virus Varicella zoster. Gen HLA-B27 ditemukan pada enam subjek penelitian dan terdapat kesesuaian dengan kasus uveitis non-infeksi tetapi belum dapat dibuktikan kemaknaannya secara statistik.
Uveitis is an intraocular inflammation which occurs on the uvea or its surrounding tissue such as retina or vitreous. Uveitis is known as the third major cause of blindness in the world with the highest prevalence is productive age group 20 50 years old . To date, infectious and non infectious uveitis stipulation in Indonesia is based on clinical examination and serology test. The aqueous and serum antibody titer measurement as well as genetic marker examination, especially on HLA B27, has never been done before. In this research we perform HLA B27 detection through molecular analysis and antibody titer measurement to know the Goldmann Whitmer coefficient. Sample size used in this research is 79 patients with active uveitis obtained from ldquo Poliklinik Infeksi dan Imunologi RSCM Kirana rdquo . The analysis of Goldmann Whitmer coefficient showed that the pathogens responsible for uveitis are Toxoplasma gondii and Varicella zoster. HLA B27 were found in six patients and there is similiarity between non infectious uveitis but this value does not have prove statistically. "
2018
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UI - Tesis Membership  Universitas Indonesia Library
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Dhany Nugraha Ramdhany
"Sistem urinari hewan dapat dibagi menjadi 2 bagian yaitu sistem urinari bagian atas dan sistem urinari bagian bawah. Ginjal yang merupakan bagian dari sistem urinari memiliki 2 fungsi penting, yaitu filtrasi dan reabsorpsi. Dalam mendiagnosis penyakit yang diderita hewan pada sistem urinarinya terdapat beberapa kendala. Pada penelitian ini, dikembangkan model untuk mendiagnosis gangguan sistem urinari pada anjing dan kucing dengan menggunakan algoritma VFI 5 berdasarkan gejala klinis (terdapat 37 feature) dan pemeriksaan laboratorium (39 feature). Percobaan dilakukan baik pada feature gejala klinis dan juga pada feature pemeriksaan laboratorium. Hasil pengamatan yang dilakukan menunjukkan bahwa akurasi rata-rata sebesar 77,38% untuk percobaan dengan feature gejala klinis, dan 86,31% untuk percobaan dengan feature pemeriksaan laboratorium. Peningkatan ini mengindikasikan bahwa dalam mendiagnosis penyakit dalam sistem urinari, pemeriksaan laboratorium masih sangat dibutuhkan dalam menentukan hasil diagnosis suatu penyakit."
[Fakultas Ilmu Komputer Universitas Indonesia, IPB. Departemen Ilmu Komputer], 2009
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Artikel Jurnal  Universitas Indonesia Library
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Jackson, Scott M.
"When faced with a challenging dermatologic problem, physicians are often required to perform a time-consuming search through large dermatologic texts in order to find information that will assist in the necessary differential diagnosis. This comprehensive and concise handbook is designed to simplify this process dramatically, permitting rapid identification of the correct diagnosis. Hundreds of dermatologic diagnoses, morphologic features, drug-induced disorders, extracutaneous manifestations, histologic findings, and random other findings are listed in alphabetical order and in a homogeneous, reader-friendly structure. The differential diagnoses are shown under each main diagnosis, sorted according to similarity with that diagnosis. Since the first edition, approaching 50 new diagnoses have been added, and many new images included. "
Berlin : Springer, 2012
e20425889
eBooks  Universitas Indonesia Library
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Scully, Crispian
London: Martin Dunitz, 1999
R 617.522 SCU h
Buku Referensi  Universitas Indonesia Library
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Rizqi Agung Wicaksana
"Penyakit jantung bawaan (PJB) sianotik menyebabkan terjadinya hipoksia kronik yang akan memicu peningkatan eritropoietin dan meningkatkan kadar hemoglobin (Hb). Diagnosis defisiensi besi pada PJB sianotik menjadi sulit karena parameter standar seperti Hb dan indeks eritrosit tidak dapat digunakan. Parameter pemeriksaan lain seperti feritin dan saturasi transferin sering tidak akuirat karena dipengaruhi berbagai faktor seperti inflamasi akut. American Academy of Pediatrics merekomendasikan pemeriksan reticulocyte hemoglobin equivalent (Ret-He) sebagai alat diagnosis defisiensi besi yang tidak dipengaruhi inflamasi akut. Serial kasus ini bertujuan untuk menilai gambaran Ret-He dalam menilai defisiensi besi pada pasien PJB sianotik. Sebanyak 11 subjek berusia 6 bulan - 5 tahun dengan PJB sianotik mengikuti penelitian ini dan dilakukan pemeriksaan antropometri, saturasi oksigen, Hb, indeks eritrosit, serum besi, total iron binding complex (TIBC), feritin, dan Ret-He. Terdapat 7 subjek dengan diagnosis tetralogi Fallot dan 4 subjek dengan double-outlet right ventricle (DORV). Sebanyak 10 subjek mengalami malnutrisi dan gangguan penyimpanan besi, namun status gizi tidak berbanding lurus dengan status besi. Nilai Ret-He yang rendah ditemui pada 9 dari 10 subjek yang mengalami gangguan penyimpanan besi meskipun tidak ditemukan nilai yang linear. Berdasarkan temuan di atas, diperlukan beberapa pemeriksaan laboratorium dalam mendiagnosis defisiensi besi pada PJB sianotik.

Cyanotic congenital heart disease (CHD) would cause chronic hypoxia and trigger erythropoietin which led to increase hemoglobin (Hb) levels. Diagnosis of iron deficiency in cyanotic CHD is difficult because standard parameters such as Hb and erythrocyte index cannot be used. Another parameters like ferritin and transferrin saturation are confounded by various factor such as acute inflammation. The America Academy of Pediatrics recommends reticulocyte hemoglobin equivalent (Ret-He) as a diagnostic tool for iron deficiency which not influenced by acute inflammation. This case series aims to assess the role of Ret-He in diagnosing iron deficiency in cyanotic CHD. A total 11 subjects aged 6 months - 5 years with cyanotic CHD included in this study and were examined to anthropometric, oxygen saturation, Hb, erythrocyte index, serum iron, total iron binding complex (TIBC), ferritin, and Ret-He. There were 7 subjects diagnosed with tetralogy of Fallot’s and 4 subjects with double outlet right ventricle (DORV). Malnutrition found and iron storage disorder was found in 10 subject, but nutritional status was not directly proportional to iron status. A low Ret-He value was found in 9 out of 10 iron-deficient subjects, although no linearity between them. Based on the above findings, several laboratory tests are needed in diagnosing iron deficiency in cyanotic CHD.
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Jakarta: Fakultas Kedokteran Universitas Indonesia, 2020
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UI - Tesis Membership  Universitas Indonesia Library
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Stewart, Michael G
"Abstract:
Designed as a practical resource for rapid and accurate diagnosis in otolaryngology--head and neck surgery and facial plastic surgery, this comprehensive manual uses an innovative format that simulates what physicians experience in daily practice."
New York: Thieme, 2011
617.510 STE d
Buku Teks  Universitas Indonesia Library
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New Delhi: Jaypee Brothers, 2008
616.31 ORA
Buku Teks  Universitas Indonesia Library
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