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"Handajani NS, Dharmawan R. 2009. Effect of VCO to leucocyte differential count, glucose levels and blood creatinine ofhyperglycemic and ovalbumin sensitized Mus musculus Balb/c. Nusantara Bioscience 1: 1-8. Chemical medicines and insulin candecrease blood glucose level in hyperglycemic patients with a macro vascular side effect. Diabetes and allergy incidences are influencedby quality and quantity of leucocytes. Lauric acid within VCO reports decreased blood glucose level of diabetes and some allergyincidents. The purpose of the study is to know the effect of VCO on blood glucose level, differential leucocytes count and bloodcreatinine level on hyperglycemic and normoglycemic ovalbumin-sensitized mice. Forty-five (45) male (mice) of Mus musculus Balb/cwith an average weight of 35 g are divided into nine groups with five repetitions; those are four non-alloxan groups and five alloxaninduced hyperglycemic groups. On 22nd day to 36th day, they are sensitized to ovalbumin as an allergen. A blood sample was obtainedby orbital vena using heparin as anticoagulant in order measuring blood glucose level by GOD method to 6 times, on 1st, 4th, 18th, 22nd,32nd and 37th days, then are tested by ANOVA followed by DMRT 0.05. On 37th day, differential leucocytes are determined, blood levelare counted, and then compared to normal value. The results of this study were that within differential leucocytes count ofhyperglycemic mice, neutrophil percentage were much lower than the normal value (3.22%), and lymphocyte percentage were muchhigher than the normal value (94.54%). Consumed 0.003 mL/35 g VCO more 18 days decreased blood glucose level on hyperglycemicmice, decreased basophil percentage of ovalbumin-sensitized mice, normalized neutrophil percentage no increased creatinine bloodlevel. "

"ABSTRAKLatar belakang. Rinitis Alergi adalah penyakit inflamasi yang disebabkan oleh reaksi alergi pada pasien atopi. Tungau debu rumah TDR merupakan aeroalergen tersering yang mensensitisasi reaksi alergi. Pada tahun 1988 reseptor vitamin D VDR , berhasil di klon. Reseptor vitamin D berlokasi di beberapa jaringan dan sel tubuh manusia, termasuk di peripheral blood mononuclear cells PBMCs dan limfosit T yang telah teraktivasi. Riset yang lebih jauh memperlihatkan bahwa vitamin D mempunyai beberapa efek dari pengaturan sitokin terhadap beberapa sel yang berbeda dari sistem imun. Vitamin D dapat menekan respon Th1 dan Th2. Sel Th1 dan Th2 menghambat perkembangan satu sama lain.Tujuan. Melihat pengaruh pemberian Vitamin D terhadap kadar IL-10, IFN-? dan histamin pada kultur PBMC pasien rinitis alergiMetode. Sampel merupakan darah segar whole blood penderita rinitis alergi yang telah dilekukan prick test, serta diiisolasi dengan metode Ficoll. Bahan biologis tersimpan yang berupa supernatan kultur. Kultur sel limfosit sebelum perlakuan, diberi pendedahan tanpa atau dengan 1,25 OH 2D3 100 nM, waktu inkubasi 7 hari, dengan penambahan PHA dan alergen tungau pada hari ke-4. Kultur sel-sel PBMC dari pasien RA setelah perlakuan, dilakukan harvest pada hari ke-7, kemudian supernatanannya dialikuot untuk diukur kadar sitokin IFN-? dan IL-10, dan diuji secara statistik untuk melihat pola dari tiap parameter.Hasil: Pemberian alergen tungau tanpa vitamin D menyebabkan meningkatnya kadar histamin serta menurunkan kadar IL-10 dan IFN-?. Pemberian vitamin D pada kultur sel PBMC yang telah diberi alergen tungau menyebabkan peningkatan kadar IL-10 dan penurunan kadar IFN-? dan histamin.Simpulan: Menurunnya kadar histamin dan IFN-? terhadap stimulasi alergen tungau pasien rinitis alergi yang diberi vitamin D cenderung berhubungan dengan meningkatnya kiadar IL-10.

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ABSTRACTBackground. Allergic rhinitis is an inflammatory disease caused by an allergic reaction in atopic patients. House dust mites TDR are the most common aeroalergens that sensitize allergic reactions. In 1988 vitamin D receptor VDR , successfully in clones. Vitamin D receptors are located in several tissues and human body cells, including peripheral blood mononuclear cells PBMCs and activated T lymphocytes. Further research has shown that vitamin D has some effects of cytokine regulation on several cells different from the immune system. Vitamin D can suppress Th1 and Th2 responses. Th1 and Th2 cells inhibit the development of each other. Aim.To identify the Effect of Vitamin D On IL 10 IFN and histamine levels on PBMC Cultur of Allergic Rhinitis PatientsMethod. The sample is fresh blood whole blood of allergic rhinitis patients who have been prick tested, and isolated by Ficoll method. Pre treated lymphocyte culture, treated with or without 1,25 OH 2D3 100 nM, and incubated for 7 days, with addition of PHA and allergen mites on day 4. Cultures of PBMC cells from RA patients after treatment were harvested on day 7, then the supernatant was dialyzed for measured levels of IFN and IL 10 cytokines using elisa kits and tested statistically to see patterns of each parameter.Result. Giving allergen mites without vitamin D causes increased levels of histamine and lowers levels of IL 10 and IFN . Provision of vitamin D in PBMC cell culture that has been given allergen mites causes increased levels of IL 10 and decreased levels of IFN and histamine.Conclusion. Reduced levels of histamine and IFN against allergen mite stimulation of allergic rhinitis patients given vitamin D tend to be associated with increased IL 10 levels."

"ABSTRAKLatar belakang. Pelayanan transfusi darah merupakan penunjang pelayanan kesehatan yang sangat penting, karena hingga saat ini masih terdapat beberapa kondisi kesehatan yang hanya dapat diatasi dengan pemberian transfusi darah. Salah satu strategi World Health Organization (WHO) dalam pelayanan darah yang aman adalah transfusi darah atas indikasi medis secara rasional. Febrile non-haemolytic transfusion reaction (FNHTR) telah dilaporkan sebagai reaksi transfusi yang paling umum terjadi dengan insidensi 6,8% setelah transfusi produk komponen packed red cell (PRC). Data di Pusat Thalassemia Rumah Sakit Dr. Cipto Mangunkusumo (RSCM) Jakarta, dari 73% pasien yang mendapat PRC leucoreduction, 15% di antaranya mengalami reaksi transfusi, sedangkan dari 14% pasien yang mendapat PRC biasa, 65% di antaranya mengalami reaksi transfusi. Di Indonesia, PRC yang tersedia umumnya adalah produk PRC leucoreduction dengan metoda buffy-coat depleted. Penelitian ini bertujuan untuk mengetahui efektifitas penurunan jumlah leukosit dan sitokin pada produk PRC dari metoda buffy-coat depleted dibandingkan dengan metoda modifikasi bed-side leucocyte filtration. Metodologi. Penelitian ini menggunakan desain potong lintang pada subjek berupa 30 produk PRC yang dibuat dengan metoda buffy-coat depleted dan 30 produk PRC yang dibuat dengan metoda modifikasi bed-side leucocyte filtration pada <48 jam masa penyimpanan. Pada semua produk dilakukan pemeriksaan hematologi dan pemeriksaan sitokin pirogen IL-6 dan TNF-α. Hasil. Satu (3,33%) subjek kantong komponen PRC yang dibuat dengan metoda buffy-coat depleted memenuhi standar leukoreduction (<5x108 leukosit/unit), dan 29 (96,7%) subjek kantong komponen PRC yang dibuat dengan metoda modifikasi bed-side leucocyte filtration pada waktu < 48 jam penyimpanan memenuhi standar leukodepleted (<5x106 leukosit/unit). Pada penelitian ini tidak didapatkan perbedaan bermakna kadar IL-6 dan TNF-α pada kedua kelompok komponen PRC ( p > 0,05 ). Simpulan. Terdapat penurunan jumlah leukosit komponen PRC yang dibuat dengan metoda modifikasi bed-side leucocyte filtration pada < 48 jam masa penyimpanan PRC sangat signifikan dibandingkan dengan metoda buffy-coat depleted. Hal ini disebabkan oleh peranan filter polyurethane yang selektif menyaring leukosit sedangkan penurunan jumlah leukosit pada metoda buffy-coat depleted dipengaruhi oleh kecepatan dan waktu putaran sentrifus serta pemisahan lapisan buffy coat dari komponen PRC. Tidak bermaknanya perbedaan kadar sitokin pirogenik IL-6 dan TNF α pada kedua kelompok PRC dikarenakan masa penyimpanan PRC < 48 jam tidak menyebabkan akumulasi sitokin pirogenik IL-6 dan dan TNF-α.

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ABSTRACTBackground. Blood transfusion is an essential part of health services, that can safe lifes. One of the World Health Organization (WHO) strategy on safe blood. White blood cells/leukocytes are present in all cellular blood components that are prepared by standard technique. Febrile non-haemolytic transfusion reaction (FNHTR) has been reported as a common transfusion reaction with the incidence of 6,8% after Packed Red Cell (PRC) transfusion. Data in Thalassemia Center DR. Cipto Mangunkusumo Hospital in Jakarta, from 73% of patients who received PRC leucoreduced component, 15% of them had a transfusion reaction, whereas 14% of patients who received PRC component, 65% of them had a transfusion reaction. In Indonesia, the common PRC component available is a leucoreduced PRC developed by buffy-coat depleted method. The study is aim to evaluate the effectiveness of leucocyte reduction and cytokine on the PRC components developed by buffy-coat depleted method compare to the PRC products developed by modified bed-side leucocyte filtration method. Methodology. The study is a cross sectional study on the subject of 30 PRC components developed by buffy-coat depleted method and 30 PRC component developed by modified bed-side leucocyte filtration method in < 48 hour of storage. Haematology testing and pyrogenic cytokine of IL-6 and TNF-α titer was analyzed on all subjects.Result. There was only one (3.33%) subject of PRC developed by buffy-coat depleted method showed to be leucoreduced (<5x108 leucocyte/unit), mean while there was 29 (96,7%) subject of PRC developed by modified bed-side leucocyte filtration method showed to be leukodepleted (<5x106 leucocyte/unit).No significant difference of IL-6 and TNF-α titer on both of PRC components. (p > 0,05 ).Conclusion. Reduction of leucocyte on the PRC components developed by modified bed-side leucocyte filtration is more effective compare to that on the PRC components developed by buffy-coat depleted method. The adhesion principle of leucocyte into polyurethane filter was more effective in reducing the number of leucocyte compare to centrifugation principle. The leucocyte filtration that was run on the PRC components with the storage time of < 48 hour did not caused the accumulation of pyrogenic cytokine such as IL-6 and TNF-α."

"ABSTRAKAsam lipoat adalah senyawa yang terkandung dalam intra dan ekstraselular yang bertindak sebagai koenzim piruvat dehidrogenase, penangkal racun, pengkat logam berat dan antioksidan. Pengukuran asam lipoat diperlukan untuk mengetahui jumlah asam lipoat yang menjalankan fungsinya baik sebagai koenzim atau antioksidan. Tetapi pengukuran ini membutuhkan alat khusus seperti HPLC dan proses yang tidak mudah. Alat yang umum dan mudah seperti spektrofotometer diharapkan bisa menjadi alat penentuan asam lipoat. Pengukuran asam lipoat menggunakan spektrofotometri dengan metanol UV dan PdCl2 terlihat telah diuji dan diperoleh hasil yang valid dan dapat diandalkan dalam sediaan obat atau farmasi tetapi belum diuji untuk plasma. Pengukuran asam lipoat dalam plasma dan leukosit menggunakan PdCl2 menghasilkan pengukuran yang valid, dengan akurasi, presisi tinggi dan tidak berbeda dengan pengukuran asam lipoat menggunakan HPLC, p= 0,99. Sedangkan UV methanol berbeda dibanding HPLC p= 0,0001 atau tidak valid.

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ABSTRACTLipoic acid is a compound contained in intra and extracellular that act as a coenzyme of Pyruvate Dehydrogenase, antidote, chelating agent and antioxidant. Measurement of lipoic acid is needed to determine the amount of lipoic acid that performs its functions either as a coenzyme or an antioxidant. But this measurement requires a special tool such as High Performance Liquid Chromatography HPLC and a process that is not easy. A common and easy tool such as a spectrophotometer is expected to be a tool of lipoic acid determination. Measurement of lipoic acid using spectrophotometry with UV methanol and visible PdCl2 has been tested and obtained valid and reliable results in drug or pharmaceutical preparations but not yet tested for plasma. Determination of lipoic acid in plasma and leukocytes using PdCl2 produced valid result, with high accuracy, precision and was not different from lipoic acid measurement using HPLC, p 0.99. While UV methanol was different compare to HPLC p 0.0001 or was not valid."

"ABSTRAKLatar Belakang : Nusa Tenggara Timur adalah wilayah endemik malaria. Tidak hanya malaria simtomatik, malaria asimtomatik juga menjadi beban daerah ini. Pendeteksiannya harus dilakukan dengan seksama. Perubahan hematologi yang terjadi pada pasien, diantaranya jumlah dan hitung jenis leukosit dapat dijadikan salah satu penanda terjadinya malaria asimtomatik. Tujuan : Tujuan dari penelitian ini adalah mengetahui prevalensi malaria asimtomatik, mengetahui profil jumlah dan hitung jenis leukosit, serta mengetahui hubungan malaria asimtomatik dengan jumlah dan hitung jenis leukosit pada warga di Kecamatan Nangapanda, Kabupaten Ende, NTT. Metode : Metode penelitian ini adalah potong lintang dengan sampel yang berasal dari data sekunder penelitian induk di Nangapanda, Ende, NTT pada tahun 2009. Hasil : Prevalensi malaria asimtomatik di Kecamatan Nangapanda, Ende, NTT sebesar 14,7 . Median nilai minimum-nilai maksimum jumlah leukosit = 8000 3000-21000 sel/mm3, hitung jenis monosit = 5 0-14 , hitung jenis granulosit = 47 10-71 , hitung jenis limfosit = 48 23-84 . Pada penelitian ini tidak ditemukan adanya hubungan malaria asimtomatik dengan jumlah dan hitung jenis leukosit pada keseluruhan data. Namun, jika dikelompokkan berdasarkan jenis kelamin, terdapat hubungan signifikan antara malaria asimtomatik dengan monosit pada jenis kelamin laki-laki p=0,031 . Diskusi : Tidak terdapat hubungan signifikan antara infeksi malaria asimtomatik dengan jumlah dan hitung jenis leukosit pada warga di Kecamatan Nangapanda, Kabupaten Ende, NTT.

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ABSTRACTIntroduction East Nusa Tenggara is a malaria rsquo s endemic region. The region bears not only the burden of symptomatic malaria, but also asymptomatic malaria. The detection of this disease must be done carefully, and one of the methods is to measure the hematological changes that happened in the patients rsquo body, calculating the total and differential leukocyte counts. Objective The objectives of this study were to find out the prevalence of asymptomatic malaria, the total and differential counts of leukocyte, and also the relationship between asymptomatic malaria with the total and differential leukocyte counts on the residents of Nangapanda, Ende, East Nusa Tenggara. Method This study used cross sectional method, with the sample originated from a secondary data of the previous study in Nangapanda, Ende, East Nusa Tenggara back in 2009. Result The prevalence of asymptomatic malaria in Nangapanda, Ende, East Nusa Tenggara was 14.7 . While the median minimum maximum total leukocyte counts 8000 3000 21000 cell mm3, monocyte counts 5 0 14 , granulocyte counts 47 10 71 , lymphocyte counts 48 23 84 . In this study, there was no significant relationship between asymptomatic malaria with total and differential leukocyte counts in the overall data. However, if categorized by sex, there was a significant relationship between asymptomatic malaria and monocyte counts in male p 0.031 . Discussion There was no significant relationship between asymptomatic malaria infection with the total and differential counts of leukocyte on residents of Nangapanda, Ende, East Nusa Tenggara. "

"ABSTRAKSalah satu jenis kanker yang banyak ditemukan di Indonesia adalah kanker serviks. Berdasarkan urutan frekuensi penderita, kanker serviks menduduki tempat teratas dibandingkan kanker ginekologi lainnya pada wanita. Di dalam tubuh, sel kanker akan ditolak oleh reaksi imunitas selular, yang dilakukan oleh limfosit T. Reaksi imunitas selular dapat diuji secara in vitro berdasarkan kemampuan limfosit bertransformasi akibat adanya "phytohemagglutinin". Pada penderita kanker kemampuan limfosit untuk bertransformasi menurun. Dalam penelitian ini dilakukan pemeriksaan reaksi imunitas selular dari 30 orang wanita penderita kanker serviks yang akan berobat di Sub-bagian Radioterapi RSCM dan 30 orang wanita yang tidak menderita kanker serviks, maupun kanker lainnya, dengan cara mengkultur darah kemudian dihitung indeks stimulasinya. Selain itu juga diperiksa jumlah limfosit dan jumlah leukosit. Dengan uji t, diperoleh kesimpulan adanya penurunan reaksi imunitas seluler (indeks stimulasi) dan jumlah limfosit pada wanita penderita kanker serviks (a = 0,01). Sedangkan jumlah leukosit tidak menurun jika dibandingkan dengan wanita yang tidak menderita kanker serviks maupun kanker lainnya (a = 0,01)"

"ABSTRAK Latar Belakang Leukemia limfoblastik akut LLA adalah keganasan paling sering pada anak di sebagian besar dunia insiden bervariasi di berbagai daerah mulai 15 sampai 40 Keberhasilan pengobatan pada LLA dapat dilihat berdasarkan angka kesintasan Rumah Sakit Sanglah telah merawat pasien leukemia anak akan tetapi sampai saat ini belum pernah dilakukan penilaian terhadap kesintasan pada kasus leukemia akut Tujuan Untuk mengetahui angka kesintasan pasien LLA serta faktor faktor yang memengaruhi LLA di bawah umur 12 tahun di RSUP Sanglah dari tahun 2010 2012 Metode Penelitian kohort retrospektif dilakukan di RS Sanglah dengan menggunakan data sekunder dari catatan medis pasien LLA dari Januari 2010 ndash Desember 2012 Sampel adalah pasien berusia 0 12 tahun terdiagnosis LLA kemudian dikelompokkan menjadi risiko biasa dan risiko tinggi berdasarkan nilai leukosit awal usia dan protokol LLA tahun 2006 Hasil Penelitian Terdapat 33 subjek pasien LLA Didapatkan perbedaan bermakna faktor prognostik usia 1 9 tahun dengan usia 9 tahun dan jumlah leukosit 50 000 mL dengan leukosit 50 000 mL memengaruhi angka kesintasan dengan nilai masing masing p 0 023 dan p 0 013 Angka kesintasan hidup secara keseluruhan pasien LLA adalah 30 3 didapatkan perbedaan bermakna angka kesintasan antara RT dan RB dengan nilai masing masing adalah 11 8 43 8 dan p ABSTRACT Background Acute lymphoblastic leukemia ALL is the most common hildhood cancer in the world Incidence rate found various in several countries from 15 to 40 A successful theraphy of ALL be evaluated by the survival rate Sanglah hospital has been treated children with ALL but however a research of survival rate in children with ALL has never been done before Objective To know the survival rate of children with ALL and factors that affect ALL in children under 12 years old that has been treated in Sanglah Hospital from 2010 2012 Method A retrospective cohort study run in Sanglah Hospital using secondary data from medical record of children with ALL between January 2010 December 2012 Sample is ALL patients aged 0 12 years old with diagnosis ALL will be separated into normal risk group and high risk group based on early number of leucosyte age and therapy protokol year 2006 Results There are 33 subjects of children with ALL Significant difference of prognostic factors were found between the age of 1 9 years old and at the age 9 years old as well as between leucocyte count 50 000 mL and those with leucocyte count 50 000 mL affect the survival rate with each p value of p 0 023 and p 0 013 The Overall survival rate of ALL patients was 30 3 There were significant difference of survival rate between RT and RB valued 11 8 43 8 with p;Background Acute lymphoblastic leukemia ALL is the most common hildhood cancer in the world Incidence rate found various in several countries from 15 to 40 A successful theraphy of ALL be evaluated by the survival rate Sanglah hospital has been treated children with ALL but however a research of survival rate in children with ALL has never been done before Objective To know the survival rate of children with ALL and factors that affect ALL in children under 12 years old that has been treated in Sanglah Hospital from 2010 2012 Method A retrospective cohort study run in Sanglah Hospital using secondary data from medical record of children with ALL between January 2010 December 2012 Sample is ALL patients aged 0 12 years old with diagnosis ALL will be separated into normal risk group and high risk group based on early number of leucosyte age and therapy protokol year 2006 Results There are 33 subjects of children with ALL Significant difference of prognostic factors were found between the age of 1 9 years old and at the age 9 years old as well as between leucocyte count 50 000 mL and those with leucocyte count 50 000 mL affect the survival rate with each p value of p 0 023 and p 0 013 The Overall survival rate of ALL patients was 30 3 There were significant difference of survival rate between RT and RB valued 11 8 43 8 with p;Background Acute lymphoblastic leukemia ALL is the most common hildhood cancer in the world Incidence rate found various in several countries from 15 to 40 A successful theraphy of ALL be evaluated by the survival rate Sanglah hospital has been treated children with ALL but however a research of survival rate in children with ALL has never been done before Objective To know the survival rate of children with ALL and factors that affect ALL in children under 12 years old that has been treated in Sanglah Hospital from 2010 2012 Method A retrospective cohort study run in Sanglah Hospital using secondary data from medical record of children with ALL between January 2010 December 2012 Sample is ALL patients aged 0 12 years old with diagnosis ALL will be separated into normal risk group and high risk group based on early number of leucosyte age and therapy protokol year 2006 Results There are 33 subjects of children with ALL Significant difference of prognostic factors were found between the age of 1 9 years old and at the age 9 years old as well as between leucocyte count 50 000 mL and those with leucocyte count 50 000 mL affect the survival rate with each p value of p 0 023 and p 0 013 The Overall survival rate of ALL patients was 30 3 There were significant difference of survival rate between RT and RB valued 11 8 43 8 with p"

"Kanker payudara masih menjadi jenis kanker yang paling umum terjadi di dunia. Kanker payudara merupakan penyakit kompleks yang disebabkan oleh berbagai faktor. Pemeriksaan histopatologi dapat memberikan informasi penting mengenai fenotipe, yaitu karakteristik fisik atau morfologi dari jaringan yang diperiksa. Pemeriksaan genotipe juga dapat dilakukan untuk mengidentifikasi varian gen pada mutasi gen tertentu, yang dapat memberikan informasi tentang faktor risiko genetik seseorang terhadap penyakit tertentu dan respons terhadap terapi target yang ditujukan pada mutasi gen tertentu. Penelitian ini bertujuan untuk mengidentifikasi mutasi gen penetrasi non-BRCA dan hubungan antara genotipe-fenotipe pada pasien kanker payudara. Pada penelitian ini, pemeriksaan genotipe dilakukan menggunakan metode targeted sequencing. Pada penelitian ini ditemukan sebanyak 18 gen kerentanan dengan varian pathogenic dan likely-pathogenic (P/LP). Kelompok varian gen dibandingkan dengan fenotipe pasien yaitu diantaranya adalah usia, riwayat kanker payudara pada keluarga, status metastasis, subtipe molekuler, dan grade. Kesimpulannya, ditemukan mutasi gen penetrasi non-BRCA diantaranya SMAD4 H427Lfs*9, ATM H1951Pfs*39, PTEN Q219Rfs*2, dan MET K842Sfs*7, mutasi gen penetrasi SMAD4 H427Lfs*9 berhubungan dengan subtipe molekuler luminal B, mutasi gen penetrasi ATM H1951Pfs*39, PTEN Q219Rfs*2, dan MET K842Sfs*7 berhubungan dengan subtipe molekuler TNBC. Pada penelitian ini juga dilakukan homology modelling protein PTEN mutan terhadap protein PTEN wild type dan kaitannya dengan respons terapi target GSK2636771 dan AZD8186.

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Breast cancer is still the most common type of cancer in the world. Breast cancer is a complex disease caused by various factors. Histopathological examination can provide important information regarding the phenotype, namely the physical or morphological characteristics of the tissue being examined. Genotyping tests can also be performed to identify gene variants in certain gene mutations, which can provide information about a person's risk of genetic factors for certain diseases and response to targeted therapy aimed at certain gene mutations. This study aims to identify non-BRCA penetrance gene mutations and the relationship between genotypes in breast cancer patients. In this study, genotype examination was carried out using the targeted sequencing method. In this study, 18 susceptibility genes with pathogenic and likely-pathogenic (P/LP) variants were found. Gene variant groups were compared with the patient's phenotype, including age, family history of breast cancer, metastatic status, molecular subtype, and grade. In conclusion, non-BRCA penetrance gene mutations were found, including SMAD4 H427Lfs*9, ATM H1951Pfs*39, PTEN Q219Rfs*2, and MET K842Sfs*7. SMAD4 H427Lfs*9 penetrance gene mutation is associated with luminal molecular subtype B, ATM H1951Pfs penetrance gene mutation *39, PTEN Q219Rfs*2, and MET K842Sfs*7 are associated with TNBC molecular subtypes. In this study, homology modeling was also performed on wild type PTEN protein with mutant PTEN protein and its relation to the response to targeted therapy of GSK2636771 and AZD8186."

"ABSTRAKTungau debu rumah merupakan sumber alergen yang dapat menyebabkan alergi. Menurut biofisika, terjadinya alergi dalam tubuh manusia adalah hasil munculnya pencetakan alergi allergy imprinting yang berasal dari kontak tubuh manusia dengan zat dan berkembang dari pencetakan biofisik dengan substansi informasi. Penelitian dirancang dengan desain eksperimental untuk menilai dampak bioresonansi gelombang elektromagnetik terhadap perubahan profil mediator pro inflamasi type 2 IL4 dan IL13 dan mediator anti inflamasi IL10 yang dihasilkan oleh kultur sel darah lengkap yang diambil dari subjek alergi rhinitis karena tungau debu rumah. Hasil menunjukkan bahwa pada group dengan perlakuan bioresonansi didapatkan perbedaan. IL 4 dan IL13 yang diberi antigen tungau terhadap kontrol negatif lebih rendah dibandingkan dengan group yang tanpa perlakuan bioresonansi. Kadar IL10 sebagai mediator anti inflamasi lebih meningkat dibandingkan dengan group tanpa perlakuan bioresonansi. Hasil analisa respon sel darah lengkap yang menunjukkan kenaikan dari kondisi kontrol RPMI dibandingkan terhadap PHA Phytohemagglutinin menggambarkan bahwa kelompok dengan perlakuan bioresonansi dan yang tidak diberi perlakuan bioresonansi pada hari ke-7 masih hidup dan menghasilkan produksi sitokin. Produksi meditor akibat perlakuan bioresonansi gelombang elektromagnetik tidak mengubah fungsi biologik dari peran anti inflamasi yang secara fungsional dapat menghambat laju produksi inflamasi. Kata kunci: alergi tungau debu rumah, bioresonansi, interleukin

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ABSTRACTHouse dust mites are a source of allergens that can cause allergies. In the view of biophysics the occurrence of allergies in the human body is the result of the emergence of allergy imprinting which comes from human body contact with substances and evolves from biophysical printing with the substance of information. The study was designed with an experimental design to assess the impact of bioresonance of electromagnetic waves on changes in pro inflammatory mediator profiles IL 4 and IL 13 and anti inflammatory mediators IL 10 produced by complete blood cell cultures drawn from subjects of rhinitis allergy due to dust mites home. The result shows that the group given the bioresonance treatment, the difference between IL 4 and IL 13 given the mite antigen to negative control is lower than that of group without treatment. Level of IL 10 as an anti inflammatory mediator is increased compared to the group without bioresonance treatment. Result of a complete blood cell response analysis which shows an increase in control condition RPMI compared to PHA Phytohemagglutinin illustrates that the exposed group and non exposed to bioresonance on the 7th day are viable and produce cytokine production. The production of meditators due to the exposure of programmed bioresonance treatment does not alter the biological functioning of the anti inflammatory role that functionally is able to inhibit the rate of inflammatory production. "