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"Marine-derived fungi have proven to be a rich source of cytotoxic compounds for the development of new anti cancer drugs. The aims of this research were to: 1) screen cytotoxic activity of marine fungi from Indonesian waters, 2) indentify marine fungus that produced thatproduced the most active cytotoxic compound and 3) investigate inhibition concentration 50 (IC50) value of cytotoxic compound. The fungi were isolated from marine organism collected fromWakatobi Marine National Park-South East Sulawesi, Binuangeun Beach-Banten, Manado watersNorth Sulawesi and Kepulauan Seribu Marine National Park-Jakarta. Liquid cultures of the fungiwere carried out in Malt Extract Broth and Soluble Starch Soytone medium for 4 weeks at 27?28oC without shaking. Molecular identification of fungus was conducted through PCR amplificatin usingprimers of ITS1 and ITS4 primer. Cytotoxic activity of the extract was tested by using MTT (3-(4.4-dimethylthiazol-2-yl)-2.5-diphenyl-tetrazolium bromide) method. The MTT test showed that MFW39strain exhibited the strongest cytotoxic activity. Molecular identification revealed that MFW39 marine fungus was similar to Emericella nidulans with precent identity of 99%. Mycelium and broth extract of MFW39 fungus inhibited the growth of T47D cell with IC 50 values of 21.9 and 169.3 µg/mL, respectively. Further research will be focus on to the strain of MFW39 marine fungi."

"Kanker paru-paru merupakan salah satu jenis kanker yang paling banyak diderita, terutama di negara dengan jumlah perokok dan tingkat polusi yang tinggi seperti Indonesia. Dalam beberapa tahun belakangan, perkembangan obat kanker paru jenis karsinoma bukan sel kecil diarahkan pada pengobatan multitarget karena pada pengobatan target tunggal sering kali proliferasi sel tumor dapat diaktifkan kembali melalui jalur yang lain seperti angiogenesis. Eksplorasi senyawa bioaktif dari bahan laut, termasuk fungi laut, telah mendapat perhatian khusus akhir-akhir ini sebagai pengobatan antikanker. Pada penelitian ini dilakukan pembuatan pangkalan data dan penapisan secara in silico untuk memperoleh senyawa aktif fungi laut yang berpotensi sebagai inhibitor EGFR-TK dan VEGFR2 kinase, yang masing-masing berperan sebagai agen antiproliferatif dan antiangiogenesis, menggunakan AutoDock dan Vina. Berdasarkan hasil penapisan, didapatkan tiga senyawa aktif sebagai inhibitor EGFR-TK (FU0015, FU0051, dan FU0202), satu senyawa aktif sebagai inhibitor VEGFR-2 kinase (FU0033), serta 17 senyawa potensi inhibitor multitarget (FU0018, FU0019, FU0028, FU0034, FU0037, FU0038, FU0029, FU0043, FU0079, FU0105, FU0127, FU0155, FU0156, FU0158, FU0248, FU0254, FU0261). Seluruh senyawa aktif tersebut berasal dari filum Ascomycota sehingga diharapkan dapat dilakukan eksplorasi lebih lanjut fungi laut dari filum Ascomycota.

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Lung cancer is one of the most common type of cancer, especially in countries with high numbers of smokers and high levels of pollution such as Indonesia. In the past few years, drug development of non-small cell lung cancer has been directed to a multitarget treatment as oftentimes on a single-targeted treatment proliferation of tumor cells can be reactivated via other pathways such as angiogenesis. Exploration of bioactive compounds from marine materials, including marine fungi, for anticancer treatment has become a major concern lately. In this research, database was created and in silico screening was conducted to obtain potential marine fungi active compounds as EGFR-TK and VEGFR2 kinase inhibitor, for each act as antiproliferative and antiangiogenesis agents, by using AutoDock and Vina. Based on these screening results have been identified three active compounds as the inhibitor of EGFR-TK (FU0015, FU0051, and FU0202), one active compound as the inhibitor of VEGFR-2 kinase (FU0033), and 17 compounds that potentially become a multitarget inhibitor (FU0018, FU0019, FU0028, FU0034, FU0037, FU0038, FU0029, FU0043, FU0079, FU0105, FU0127, FU0155, FU0156, FU0158, FU0248, FU0254, FU0261). All of these active compounds are from Ascomycota phylum so that further marine fungi exploration can be conducted from Ascomycota phylum."

"Menurut International Agency for Research on Cancer IARC, penderita kanker paru telah mencapai 1,8 juta jiwa dan 85 berkontribusi pada kanker paru jenis karsinoma bukan sel kecil. Dalam beberapa tahun belakangan, penelitian terhadap terapi tertarget sedang dikembangkan karena terbukti memiliki hasil terapi yang lebih efektif dan kemungkinan efek samping yang lebih sedikit. Perkembangan penelitian terhadap biota fungi laut belum banyak dieksplorasi dalam pengobatan kanker paru bukan sel kecil. Pada penelitian ini dilakukan simulasi dinamika molekuler pada senyawa biota fungi laut hasil penapisan virtual terhadap makromolekul EGFR FU0015, FU0051, dan FU0202 dan VEGFR-2 FU0033 untuk melihat aktivitas inhibisi sebagai agen antiproliferatif dan antiangiogenesis menggunakan AutoDock dan AMBER dalam suhu 300K dan 310K yang dibandingkan dengan kontrol positif EGFR Gefitinib, Erlotinib, and Imatinib dan VEGFR-2 Nikotinamid dan Vatalanib. Hasil simulasi dinamika molekuler terhadap inhibitor EGFR pada suhu 310K menunjukkan energi bebas MMGBSA dan okupansi ikatan hidrogen tertinggi dimiliki oleh FU0051 -43,72 kkal/mol; 98,80 diikuti oleh FU0202 -31.64 kkal/mol; 49,35, dan FU0015 -15,55 kkal/mol; 3,35. Fungi FU0033 sebagai bahan uji inhibitor VEGFR-2 pada suhu 310K memiliki nilai energi bebas MMGBSA yang lebih besar dibandingkan kontrol positifnya dan okupansi ikatan hidrogen yang rendah 0,15. Data penelitian menunjukkan senyawa FU0051 dan FU0202 memiliki potensi untuk menjadi calon agen antiproliferasi sehingga layak diuji in vitro.

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According to International Agency for Research on Cancer IARC, the number of lung cancer patients has reached 1,8 million lives and 85 of the number contribute to non small cell lung cancer. In the past years, research on targeted therapy has been developed due to its efficacy and a small number of side effects. Research on marine fungi compounds has not been explored to non small cell lung cancer therapy. This research uses molecular dynamic simulation method to marine fungi compounds that have been docked to EGFR FU0015, FU0051, FU0202 and VEGFR 2 FU0033 as antiproliferative and antiangiogenetic agent by inhibition activity using AutoDock and AMBER at 300K and 310K temperature using EGFR Gefitinib, Erlotinib, and Imatinib and VEGFR 2 Nicotinamide and Vatalanib as reference standards. Molecular dynamics results for EGFR inhibitors at 310K shows the best MMGBSA free energy and hydrogen occupancy in FU0051 43,72 kcal mol 98,80 followed by FU0202 31.64 kcal mol 49,35 , and FU0015 15,55 kcal mol 3,35. FU0033 fungi as a material for VEGFR 2 inhibitor shows higher MMGBSA free energy in compare to its reference standards and low hydrogen occupancy 0,15 at 310K. This research shows that FU0051 and FU0202 have potential to be an antiproliferative agent candidate, hence in vitro test should be obtained."

"[Telah dilakukan penelitian deteksi gen alkana monooksigenase (alkB) pada bakteri laut di Perairan Pulau Pari Kepulauan Seribu, Jakarta. Penelitian bertujuan untuk memperoleh isolat bakteri yang membawa gen alkB dari perairan Pulau Pari Kepulauan Seribu, Jakarta. Penelitian dilakukan selama 5 bulan sejak bulan Februari 2015 sampai bulan Mei 2015 dengan metode Polymerase Chain Reaction (PCR) pada 81 isolat yang telah diremajakan. Isolat bakteri diremajakan menggunakan medium marine agar (MA) dengan metode kuadran streak. Hasil deteksi mendapatkan satu isolat yang membawa gen alkB yaitu isolat nomor 71. Hasil amplifikasi isolat 71 menghasilkan pita DNA dengan ukuran 550 pb. Pita DNA dengan panjang 550 pb merupakan gen alkB. Hasil dari sekuensing menunjukkan bahwa Isolat 71 adalah dari spesies Bordetella sp.;Detection gene alkane monooxygenases (alkB) from marine bacteria in Pari Island Kepulauan Seribu, Jakartahas been researched. The research aims to obtain bacterial isolates that carry the gene alkBin Pari Island Kepulauan Seribu, Jakarta. The study was conducted during the five months from February 2015 to May 2015 with a method of Polymerase Chain Reaction (PCR) from 81 isolates that have been rejuvenated. Bacterial isolates rejuvenated using marine medium agar (MA) with the quadrant streak method. Obtain detection results of the isolates that carry the gene which isolates number 71. alkB amplification results of 71 isolates produce ribbon DNA with size 550 bp. DNA tape with a length of 550 bp is alkB gene.The results of sequencing showed that the isolate 71 is Bordetella sp.;Detection gene alkane monooxygenases (alkB) from marine bacteria in Pari Island Kepulauan Seribu, Jakartahas been researched. The research aims to obtain bacterial isolates that carry the gene alkBin Pari Island Kepulauan Seribu, Jakarta. The study was conducted during the five months from February 2015 to May 2015 with a method of Polymerase Chain Reaction (PCR) from 81 isolates that have been rejuvenated. Bacterial isolates rejuvenated using marine medium agar (MA) with the quadrant streak method. Obtain detection results of the isolates that carry the gene which isolates number 71. alkB amplification results of 71 isolates produce ribbon DNA with size 550 bp. DNA tape with a length of 550 bp is alkB gene.The results of sequencing showed that the isolate 71 is Bordetella sp., Detection gene alkane monooxygenases (alkB) from marine bacteria in Pari Island Kepulauan Seribu, Jakartahas been researched. The research aims to obtain bacterial isolates that carry the gene alkBin Pari Island Kepulauan Seribu, Jakarta. The study was conducted during the five months from February 2015 to May 2015 with a method of Polymerase Chain Reaction (PCR) from 81 isolates that have been rejuvenated. Bacterial isolates rejuvenated using marine medium agar (MA) with the quadrant streak method. Obtain detection results of the isolates that carry the gene which isolates number 71. alkB amplification results of 71 isolates produce ribbon DNA with size 550 bp. DNA tape with a length of 550 bp is alkB gene.The results of sequencing showed that the isolate 71 is Bordetella sp.]"

"Tumpahan minyak di perairan telah menimbulkan pencemaran di lingkungan laut maupun di daerah pesisir pantai, sehingga perlu dilakukan penelusuran kejadian tumpahan minyak dapat digunakan untuk mitigasi bencana. Penelitian ini bertujuan untuk mengetahui sebaran dan pergerakan tumpahan minyak di perairan Cilacap, Jawa Tengah tahun 2008, serta hubungannya dengan angin, arus, dan pasang surut. Identifikasi tumpahan minyak dilakukan dengan interpretasi citra Modis surface reflectance dengan melihat penurunan nilai spektral dan diikuti oleh peningkatan nilai fluorescence index. Tumpahan minyak menyebar di sekitar pesisir pantai Teluk Penyu Kecamatan Cilacap Selatan, Kecamatan Cilacap Utara, Kecamatan Kesugihan dan Kecamatan Adipala, dengan luas total area yang tercemar sebesar 1.378 ha. Angin merupakan faktor dominan yang berpengaruh terhadap pergerakan minyak dibandingkan dengan arus dan pasang surut. Pergerakan tumpahan minyak menyebar menjauhi lokasi sumber tumpahan dan bergerak searah dengan arah angin menuju ke arah timur. Dari kondisi oseanografis menunjukan bahwa jenis penyebarannya termasuk kedalam jenis difusi gabungan ekspansi dan relokasi.

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The oil spill in the waters has occurred pollution in the marine environment as well as in coastal areas, so needs to do investigation of occurrence oil spill which can use for disaster mitigation. This research is going to describe the distribution and movement of oil spill in Cilacap coastal, Central Java in 2008, and its relation with the wind, currents, and tides. Identification of oil spill conducted by Modis image surface reflectance interpretation by looking at the impairment of spectral and was followed increase in fluorescence index value. Distribution of oil spill spreading around at Teluk Penyu coast of south Cilacap, North Cilacap, Kesugihan, and Adipala, with a total area of 1.378 ha contaminated. Wind is the dominant factor influencing the movement of oil compared with currents and tides. The movement away from the spreading oil spill and the spill source location moves in the direction of the wind toward the east. Of oceanographic conditions indicate that the type of distribution, including the type of diffusion into the combined expansion and relocation."