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"ABSTRAKKarbonat hidroksiapatit merupakan suatu jenis biomaterial yang memiliki kompatibilitas tinggi dengan tulang manusia. Tujuan penelitian ini adalah untuk mempelajari pengaruh suhu dan waktu sintering untuk mendapatkan karbonat hidroksiapatit yang disintesis dengan metode hidrotermal menggunakan CaCO3 sebagai salah satu komponen. Sampel hasil sintesis dikarakterisasi dengan Fourier-transform infrared spectroscopy, x-ray diffraction, dan scanning electron microscope. Hasil FTIR menunjukkan bahwa kandungan gugus CO3 pada sampel yang dihasilkan sebanding dengan kuantitas CaCO3 yang digunakan dalam sintesis. Hasil XRD menunjukkan bahwa kandungan karbonat apatit meningkat dan kandungan kalsit menurun dengan meningkatnya suhu dan waktu sintering. Kalsit tidak terlihat pada suhu sintering 900 ̊C dan waktu sintering 2 jam.

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ABSTRACTCarbonated hydroxyapatite is a biomaterial with high biocompatibility with human bone, moreso than regular hydroxyapatite, making it an acceptable synthetic bone graft material. The purpose of this research is to study the effect of sintering temperature and time on carbonated hydroxyapatite samples synthesized using the hydrothermal method with CaCO3 as one of its components. The samples are then characterized using Fourier-transform infrared spectroscopy, x-ray diffraction, and a scanning electron microscope. IR spectra show that the CO3 content in each sample is proportional to the amount of CaCO3 used in the synthesis of said samples. Diffraction patterns from XRD show an increase in apatite content and a decrease in calcite content as sintering temperature and time increases, with temperature increases having a stronger effect on the samples than time increases. Calcite disappears completely after sintering at 900 ̊C for 2 hours."

"ABSTRAKLatar Belakang:. Scaffold komposit kitosan-hidroksiapatit-kolagen yang berasal dari cangkang kepiting serta tulang dan tendon sapi berpotensi sebagai material rekonstruksi maksilofasial yang perlu di uji Tujuan: Mengevaluasi potensi scaffold komposit kitosan-hidroksiapatit-kolagen yang ditinjau dari perubahan morfologi permukaan dan komposisinya. Metode: Spesimen scaffold direndam di dalam simulated body fluid SBF dengan suhu 37 C dalam periode 2, 4, 6, dan 8 hari. Morfologi permukaan dan komposisi scaffold dievaluasi dengan menggunakan Scanning Electron Microscope SEM dan Energy Dispersive Spectroscopy EDS . Hasil: Setelah 8 hari perendaman, terlihat partikel globular dengan diameter berkisar antara 947,6 nm 20 ?m yang membentuk lapisan pada scaffold. Porus yang terbentuk oleh partikel tersebut berdiameter 100 ?m. Persentase kalsium dan fosfor meningkat dan rasio Ca/P yang tinggi 3,82 pada scaffold yang telah direndam mengindikasikan terbentuknya lapisan hidroksiapatit. Kesimpulan: Scaffold komposit kitosan-hidroksiapatit-kolagen berpotensi sebagai material rekonstruksi maksilofasial.

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ABSTRACT
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"Penelitian ini bertujuan untuk mengamati efek implantasi scaffold Injeksi Hidroksiapatit Kitosan IHAK pada regenerasi defek tulang femur kelinci melalui gambaran histologis. Penelitian ini menggunakan kelinci New Zealand dewasa yang dibagi menjadi dua kelompok yaitu kelompok perlakuan dan kontrol, kemudian dibuat defek pada area kondil tulang femur. Defek pada kelompok perlakuan menerima implantasi scaffold IHAK dan kelompok kontrol tidak menerima apapun. Kelinci dikorbankan pada minggu ke-4 lalu dilakukan pengamatan histologis menggunakan skoring Salkeld yang telah dimodifikasi. Didapatkan hasil berupa skor histologis kelompok perlakuan adalah 4 yang menandakan tulang sudah termineralisasi dan penyatuan tulang yang sempurna. Sementara itu, skor kelompok kontrol adalah 2 yang menandakan tulang masih berbentuk kalus dengan gambaran sel kondrosit dan fibroblas. Oleh karena itu, Scaffold IHAK efektif dalam mempercepat regenerasi defek tulang femur kelinci.

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This study aimed to evaluate the effect of Injectable Hydroxyapatite Chitosan IHAC Scaffold on rabbit femoral defect regeneration by assessing its histological view. In this study, we used two adult New Zealand rabbit that divided into two groups, treated group and control group. Defect was made in femoral bone of each rabbit. The defect of treated rabbit received implantation of IHAC scaffold, while the defect of control group was left empty. Rabbit was sacrificed in the after four weeks and evaluated histologically using Modified Salkeld Histological Scoring. The results showed score for treated group was 4 which indicated mineralization and complete union of bone defect. While score for control group was 2 which indicated bone defect was still in callus stage with presence of chondrocyte and fibroblast. Therefore, Injectable Hydroxyapatite Chitosan IHAC scaffold was effective for regeneration of rabbit femoral bone defect."

"[ABSTRAKPendahuluan: Tatalaksana osteomielitis dengan debridement, guttering, pemberian antibiotika sistemik sering tidak mencapai hasil yang memuaskan dikarenakan antibiotik tidak dapat mencapai lokasi infeksi dengan baik. Cara lain dengan pemberian antibiotik lokal untuk mencapai lokasi infeksi dalam bentuk beads dinilai tidak efektif karena memerlukan 2 kali operasi. Kombinasi antibiotik lokal dengan scaffolding berupa hidroxyapatite (HA) porous yang dapat diserap dan pembawa antibiotik belum pernah diteliti.Metode penelitian: Dilakukan uji pre test post test kelompok kontrol pada model kelinci osteomielitis. Sepuluh ekor kelinci New Zealand digunakan dengan berat 2.500-3500 gr yang dibagi kedalam 2 kelompok yaitu kelompok kontrol dan kelompok perlakuan (n=5). Dilakukan penyuntikan bakteripada tulang tibia kelinci, setelah terbentuk model osteomielitis kemudian dilakukan perlakuan. Untuk kelompok kontrol dilakukan debridement dan diberikan injeksi antibiotik ceftriaxon selama 4 minggu dan kelompok perlakuan dilakukan debridement, diberikan kombinasi HA dan Gentamisin serta injeksi antibiotik ceftriaxon selama 4 minggu. Setelahnya dilakukan penilaian klinis, x- ray, kultur dan histopatologis.Temuan dan Diskusi: Secara radiologis ditemukan perbaikan skor penebalan kortek tibia pada kelompok perlakuan dibanding kelompok kontrol (p=0.48), begitu juga pada Histopatological osteomyelitis evaluation score (p=0,009). Secara klinis terdapat perbaikan skor pembengkakan pada semua (n=5) kelompok perlakuan dibanding dengan kelompok kontrol, namun secara statistik tidak bermakna (p=0,053). Sementara pada penilaian kultur tidak ditemukan perbedaan bermakna antar kedua kelompok (p=1,00)Kesimpulan: Kombinasi Hydroxyapaptite porous dan gentamisin sebagai antibiotik lokal pada pengobatan model osteomielitis tibia kelinci memberikan perbaikan skor radiologis, histopatologis dan perbaikan klinis dibanding dengan prosedur standar pengobatan oseomielitis kronis yang sudah ada.

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ABSTRACTIntroduction. Treatment of osteomyelitis with debridement, guttering, systemic antibiotics often do not achieve satisfactory results due to the antibiotic can not reach the infection site. Another way of local antibiotic delivery to reach the site of infection in the form of beads is considered ineffective because it requires two separate surgeries. Local antibiotic combined with scaffold in the form of porous hidroxyapatite that can be absorbed and antibiotics cariere have not been studied.Methods. We conducted pretest and posttest control group in a rabbit model of osteomyelitis. Ten rabbits divided in control group and the treatment group (n=5). We injected Staphylococcus aureus in the rabbit tibia, forming the osteomyelitis model, and then performed treatment for osteomyelitis. In the control group, we performed debridement and gave ceftriaxone injection for 4 weeks. Whereas in the treatment group, we add the combination of porous hidroxyapatite and Gentamicin. Afterwards, we did clinical assessment, x-ray, culture, and histopathology.Results and discussion. Radiologically, tibia cortical thickening scores improved in the treatment group compared to the control group (p=0.48) as well as histopatological osteomyelitis evaluation score (p=0,009). Clinically, there were improvements in the swelling scores (n=5) of the treatment group compared to control group, but no significant statistically (p=0.053). In culture, there were no significant difference between the two groups (p=1.00).Conclusion. Combination of porous hydroxyapaptite and gentamycin as a local treatment of osteomyelitis of the rabbit tibial osteomyelitis models improved radiological and histopathological scores and also clinically compared to existing standard treatment procedures for chronic osteomyelitis., Introduction. Treatment of osteomyelitis with debridement, guttering, systemic antibiotics often do not achieve satisfactory results due to the antibiotic can not reach the infection site. Another way of local antibiotic delivery to reach the site of infection in the form of beads is considered ineffective because it requires two separate surgeries. Local antibiotic combined with scaffold in the form of porous hidroxyapatite that can be absorbed and antibiotics cariere have not been studied.Methods. We conducted pretest and posttest control group in a rabbit model of osteomyelitis. Ten rabbits divided in control group and the treatment group (n=5). We injected Staphylococcus aureus in the rabbit tibia, forming the osteomyelitis model, and then performed treatment for osteomyelitis. In the control group, we performed debridement and gave ceftriaxone injection for 4 weeks. Whereas in the treatment group, we add the combination of porous hidroxyapatite and Gentamicin. Afterwards, we did clinical assessment, x-ray, culture, and histopathology.Results and discussion. Radiologically, tibia cortical thickening scores improved in the treatment group compared to the control group (p=0.48) as well as histopatological osteomyelitis evaluation score (p=0,009). Clinically, there were improvements in the swelling scores (n=5) of the treatment group compared to control group, but no significant statistically (p=0.053). In culture, there were no significant difference between the two groups (p=1.00).Conclusion. Combination of porous hydroxyapaptite and gentamycin as a local treatment of osteomyelitis of the rabbit tibial osteomyelitis models improved radiological and histopathological scores and also clinically compared to existing standard treatment procedures for chronic osteomyelitis.]"

"Latar Belakang: Perbaikan defek tulang dapat dilakukan dengan penggunaan material bone graft. Secara klinis, material bone graft dipakai dalam berbagai variasi ukuran granul yang disesuaikan dengan pertimbangan untuk aplikasi spesifik dan tujuan penggunaan bone graft. Di sisi lain, informasi mengenai pengaruh ukuran granul bone graft belum diketahui dan belum ada penelitian mengenai efek perbedaan ukuran granul pada prototipe karbonat hidroksiapatit terhadap kelarutan in vitro dan sitotoksisitas. Tujuan: Mengevaluasi pengaruh ukuran granul terhadap kelarutan in vitro dan sitotoksisitas prototipe karbonat hidroksiapatit. Metode: Uji kelarutan in vitro dengan merendam prototipe karbonat hidroksiapatit dalam larutan asetat buffer dan Tris-HCl buffer selama 7 hari dalam suhu 37°C. Nilai kelarutan in vitro diuji dengan alat Horiba Ion Selective Electrode. Uji sitotoksisitas dengan MTT Assay pengaruh medium ekstrak terhadap sel preosteoblas MC3T3-E1 selama 1 hari. Nilai absorbansi dibaca dengan ELISA Microplate Reader. Analisis data dengan uji statistik One-Way ANOVA. Hasil: Konsentrasi ion kalsium terlarut dalam larutan asetat buffer pada kelompok ukuran granul 250-500 µm yaitu 45,79 ± 3,11 mg/L, 500-1000 µm yaitu 37,41 ± 4,28 mg/L, dan 1000-2000 µm yaitu 35,85 ± 1,28 mg/L. Konsentrasi ion kalsium terlarut dalam larutan Tris-HCl buffer pada kelompok ukuran granul 250-500 µm yaitu 3,88 ± 0,36 mg/L, 500-1000 µm yaitu 2,94 ± 0,19 mg/L, dan 1000-2000 µm yaitu 2,02 ± 0,58 mg/L. Uji statistik menunjukkan perbedaan konsentrasi ion kalsium terlarut yang signifikan antara granul ukuran 250-500 µm dan 1000-2000 µm pada kedua larutan buffer. Persen viabilitas sel menunjukkan hasil diatas 70% pada semua kelompok ukuran granul dan konsentrasi ekstrak. Uji statistik menunjukkan perbedaan nilai absorbansi dan persen viabilitas sel pada konsentrasi ekstrak 50 mg/mL prototipe karbonat hidroksiapatit ukuran 250-500 µm terhadap 1000-2000 µm, 100 mg/mL prototipe karbonat hidroksiapatit ukuran 250-500 µm terhadap 500-1000 µm, dan konsentrasi ekstrak 200 mg/mL prototipe karbonat hidroksiapatit ukuran 250-500 µm terhadap 1000-2000 µm dan 500-1000 µm terhadap 1000-2000 µm. Kesimpulan: Semakin besar ukuran granul prototipe karbonat hidroksiapatit yang direndam dalam larutan asetat buffer dan Tris-HCl buffer, hasil uji kelarutan in vitro menunjukkan.rata-rata konsentrasi ion kalsium terlarut yang semakin kecil. Uji sitotoksisitas prototipe karbonat hidroksiapatit menunjukkan hasil nontoksik dengan viabilitas sel ukuran granul 1000-2000 µm>500-1000 µm>250-500 µm.

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Background: Repair of bone defects can be done using bone graft material. Clinically, bone graft material is used in a variety of granule sizes that are adjusted to consider the specific application and intended use of the bone graft. On the other hand, information regarding the influence of bone graft granule size is not yet known and there has been no research regarding the effect of differences in granule size in hydroxyapatite carbonate prototypes on in vitro solubility and cytotoxicity. Objective: To evaluate the effect of granule size on the in vitro solubility and cytotoxicity of the carbonate hydroxyapatite prototype. Methods: The in vitro solubility test was conducted by immersing the carbonate hydroxyapatite prototype in a solution of acetate buffer and Tris-HCl buffer for 7 days at a temperature of 37°C. The in vitro solubility value was tested using the Horiba Ion Selective Electrode. The cell cytotoxicity test was carried out using MTT assay for the effect of medium extracts on MC3T3-E1 preosteoblast cells. Absorption values were read with an ELISA Microplate Reader. Data analysis using the One-Way ANOVA statistical test. Results: The concentration of dissolved calcium ions in the acetate buffer solution in the granule size group 250-500 µm was 45.79 ± 3.11 mg/L, 500-1000 µm was 37.41 ± 4.28 mg/L, and 1000-2000 µm was 35, 85 ± 1.28mg/L. The concentration of dissolved calcium ions in the Tris-HCl buffer solution in the granule size group 250-500 µm was 3.88 ± 0,36 mg/L, 500-1000 µm was 2.94 ± 0.19 mg/L, and 1000-2000 µm was 2, 02 ± 0.58mg/L. Statistical tests showed significant differences in dissolved calcium ion concentrations between granules measuring 250-500 µm and 1000-2000 µm in both buffer solutions. The percentage of cell viability showed results above 70% in all granule size groups and extract concentrations. Statistical tests show differences in absorption values and percent cell viability at extract concentrations of 50 mg/mL of carbonate hydroxyapatite prototype with a size of 250-500 µm versus 1000-2000 µm, 100 mg/mL hydroxyapatite carbonate prototype with a size of 250-500 µm versus 500-1000 µm, and extract concentration 200 mg/mL carbonate hydroxyapatite prototype size 250-500 µm against 1000-2000 µm and 500-1000 µm against 1000-2000 µm. Conclusion: The larger the size of the carbonate hydroxyapatite prototype granules soaked in acetate buffer and Tris-HCl buffer, the results of the in vitro solubility test show a smaller average concentration of dissolved calcium ions. The cytotoxicity test of the carbonate hydroxyapatite prototype showed nontoxic results with cell viability of granule size 1000-2000 µm > 500-1000 µm > 250-500 µm."

"[ABSTRAKHidroksiapatit (HA) mempunyai peran penting dalam bidang medis karena komposisi kimia dan strukturnya yang mirip dengan tulang manusia. Material ini disintesis melalui metode hidrotermal dengan prekusor Ca(NO3)2, CaO dan NH3PO4. Variasi temperatur hidrotermal pada 150 ̊C dan 300 ̊C, temperatur sintering pada 900 ̊C selama 3 jam. Endapan yang diperoleh diuji dengan XRD dan SEM-EDX. Morfologi partikel hasil karakterisasi SEM berbentuk batangan memanjang dan melingkar teraglomerasi dan hasil uji EDX menunjukkan rasio Ca/P yang lebih besar dari 1.67. Uji XRD menunjukkan adanya fasa kalsium difosfat, fluorapatit dan apatit karbonat tipe- A di dalam endapan yang meningkatkan rasio Ca/P.ABSTRACTHydroxyapatite (HA) posseses significant role in medical application due to its similarity in chemical and structure to human bones. This material was synthesized through hydrothermal method using Ca(NO3)2, CaO and NH3PO4. Hydrothermal temperature varied on 150 ̊C and 300 ̊C, sintering temperature on 900 ̊C for 3 hours. Sample was characterization by XRD and SEM-EDX. Morphology observed by SEM is agglomerated round- spherical- shape particle with Ca/P ratio more than 1.67 measured by EDX. Calcium diphospate, fluorapatite and carbonated type- A presence is observed by XRD.;Hydroxyapatite (HA) posseses significant role in medical application due to its similarity in chemical and structure to human bones. This material was synthesized through hydrothermal method using Ca(NO3)2, CaO and NH3PO4. Hydrothermal temperature varied on 150 ̊C and 300 ̊C, sintering temperature on 900 ̊C for 3 hours. Sample was characterization by XRD and SEM-EDX. Morphology observed by SEM is agglomerated round- spherical- shape particle with Ca/P ratio more than 1.67 measured by EDX. Calcium diphospate, fluorapatite and carbonated type- A presence is observed by XRD., Hydroxyapatite (HA) posseses significant role in medical application due to its similarity in chemical and structure to human bones. This material was synthesized through hydrothermal method using Ca(NO3)2, CaO and NH3PO4. Hydrothermal temperature varied on 150 ̊C and 300 ̊C, sintering temperature on 900 ̊C for 3 hours. Sample was characterization by XRD and SEM-EDX. Morphology observed by SEM is agglomerated round- spherical- shape particle with Ca/P ratio more than 1.67 measured by EDX. Calcium diphospate, fluorapatite and carbonated type- A presence is observed by XRD.]"

"[ABSTRAKHroksiapatit merupakan jenis biomaterial sintetis yang mempunyai fasa yang paling stabil dibandingkan dengan senyawa kalsiun fosfat lainnya, selain itu hidroksiapatit memiliki tingkat kemiripan yang tinggi dengan tulang, sehingga hidroksiapatik banyak diaplikasikan sebagai bone graft sintetis. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh waktu sintering terhadap kemurnian kristal hidroksiapatit yang dihasilkan. Hidroksiapatit dari prekursor kimia CaCO3 dan (NH4)2HPO4 disintesis dengan menggunakan metode hidrotermal pada temperatur 150oC dan 300oC. Hasil dari sintesis hidroksiapatik dikarakterisasi dengan menggunakan X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), dan Energy Dispersive Analysis X-Ray (EDX). Hasil karakterisasi XRD memperlihatkan intensitas tertinggi rata-rata berada pada sudut 2θ yaitu, 25.898o, 31.789o, 32.216o, 32.922o, 46.729o, dan 49.524o. Hasil dari SEM memperlihatkan morfologi dari sampel bebrbetuk nanoroot dan hasil dari EDX menunjukan rasio Ca/P sebesar 1.8.ABSTRACTHydroxyapatite is the most phase-stable syntetic biomaterial compared to another calcium phospate material. Hydroxyapatite also has high similarity with bone which make it has wide application as syntetic bone graft. Purpose of this research is to study the effect of sintering time towards hydroxyapatite crystal?s purity. Hydroxyapatite made from chemical precursor CaCO3 and (NH4)2HPO4 was synthesized using hydrothermal method on 150oC and 300oC. Synthesized hydroxyapatite was characterized using X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Energy Dispersive Analysis X-Ray (EDX). The result of characterization showed that highest average intensity on 2θ were: 25.898o, 31.789o, 32.216o, 32.922o, 46.729o, and 49.524o.;Hydroxyapatite is the most phase-stable syntetic biomaterial compared to another calcium phospate material. Hydroxyapatite also has high similarity with bone which make it has wide application as syntetic bone graft. Purpose of this research is to study the effect of sintering time towards hydroxyapatite crystal?s purity. Hydroxyapatite made from chemical precursor CaCO3 and (NH4)2HPO4 was synthesized using hydrothermal method on 150oC and 300oC. Synthesized hydroxyapatite was characterized using X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Energy Dispersive Analysis X-Ray (EDX). The result of characterization showed that highest average intensity on 2θ were: 25.898o, 31.789o, 32.216o, 32.922o, 46.729o, and 49.524o.;Hydroxyapatite is the most phase-stable syntetic biomaterial compared to another calcium phospate material. Hydroxyapatite also has high similarity with bone which make it has wide application as syntetic bone graft. Purpose of this research is to study the effect of sintering time towards hydroxyapatite crystal?s purity. Hydroxyapatite made from chemical precursor CaCO3 and (NH4)2HPO4 was synthesized using hydrothermal method on 150oC and 300oC. Synthesized hydroxyapatite was characterized using X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Energy Dispersive Analysis X-Ray (EDX). The result of characterization showed that highest average intensity on 2θ were: 25.898o, 31.789o, 32.216o, 32.922o, 46.729o, and 49.524o., Hydroxyapatite is the most phase-stable syntetic biomaterial compared to another calcium phospate material. Hydroxyapatite also has high similarity with bone which make it has wide application as syntetic bone graft. Purpose of this research is to study the effect of sintering time towards hydroxyapatite crystal’s purity. Hydroxyapatite made from chemical precursor CaCO3 and (NH4)2HPO4 was synthesized using hydrothermal method on 150oC and 300oC. Synthesized hydroxyapatite was characterized using X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Energy Dispersive Analysis X-Ray (EDX). The result of characterization showed that highest average intensity on 2θ were: 25.898o, 31.789o, 32.216o, 32.922o, 46.729o, and 49.524o.]"

"Sumber daya litium di Indonesia salah satunya ditemukan dari brine water Gunung Panjang, Ciseeng, Bogor yang memiliki kandungan unsur litium (Li) sebesar 134,137 ppm dengan kandungan unsur kalsium (Ca) dan magnesium (Mg) yang rendah. Pada penelitian ini, brine water diberikan perlakuan distilasi dengan rasio evaporasi 80% untuk meningkatkan kandungan unsur litiumnya. Kemudian dilakukan penambahan Li2CO3 agar terjadi penghilangan unsur Ca dan Mg dengan membentuk presipitat CaCO3 dan MgCO3. Perlakuan karbonatasi kemudian diberikan pada brine menggunakan gas karbon dioksida (CO2) dengan variasi waktu karbonatasi selama 10 menit, 20 menit, 30 menit, 1 jam, dan 2 jam serta variasi temperatur karbonatasi sebesar 30oC, 40oC, dan 50oC. Karakterisasi pada penelitian ini dilakukan menggunakan ICP-OES untuk mengetahui kandungan unsur pada filtrat, XRD untuk mengetahui kandungan senyawa yang terdapat dalam residu yang dihasilkan, dan SEM-EDS untuk mengetahui komposisi elemen pada residu yang dihasilkan. Dari proses karbonatasi diketahui terdapat penurunan kandungan unsur litium serta peningkatan berat residu yang dihasilkan seiring dengan bertambahnya waktu dan meningkatnya temperatur karbonatasi. Konsentrasi unsur litium terendah didapat pada temperatur 40oC dan waktu 2 jam yaitu sebesar 102,343 ppm. Dari hasil analisis XRD diketahui bahwa endapan yang terbentuk dari proses karbonatasi adalah Li2CO3, CaCO3, Na2CO3, MgCO3, dan NaCl.

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One of the lithium resources in Indonesia can be found from the brine water of Gunung Panjang, Ciseeng, Bogor which contains 134.137 ppm lithium (Li) with low calcium (Ca) and magnesium (Mg) concentrations. In this study, brine water was treated with a distillation process with an 80% evaporation ratio to increase its lithium content. Then Li2CO3 was added to remove the Ca and Mg elements by the formation of CaCO3 and MgCO3 precipitates. Carbonation was carried out using carbon dioxide gas (CO2) with the variations of times 10 minutes, 20 minutes, 30 minutes, 1 hour, and 2 hours and variations of temperatures 30oC, 40oC, and 50oC. The characterization in this study was carried out using ICP-OES to determine the content in the filtrate, XRD to determine the compounds contained in the residue, and SEM-EDS to determine the elemental composition of the residue. The results of the carbonation process shown that there is a decrease in the lithium content and an increase in the weight of the residue produced with the increasing time and temperature in the carbonation process. The lowest elemental lithium concentration was obtained at a temperature of 40oC and a time of 2 hours, which was 102,343 ppm. From the XRD analysis, it is known that the compound formed from the carbonation process are Li2CO3, CaCO3, Na2CO3, MgCO3, and NaCl."

"Metalurgi serbuk merupakan salah satu proses produksi logam busa yang banyak digunakan untuk memperoleh struktur pori yang seragam. Dengan menggunakan variasi temperatur sinter 825°C, 850°C, 900°C dan 950°C serta variasi rata-rata ukuran partikel garam karbonat sebusar 841 m, 542 m, dan 420 m, memberikan pengaruh terhadap berbedanya struktur pori yang terbentuk pada tembaga busa pada masing-masing variabel. Penelitian ini bertujuan agar struktur pori yang terbentuk pada tembaga busa yang diproduksi dengan metoda ini dapat dikontrol sesuai dengan variabel yang digunakan. Penelitian ini menunjukkan bahwa dengan semakin tingginya temperatur sinter, maka tingkat porositas tembaga busa akan semakin menurun hingga mencapai 71,748% dan densitasnya akan semakin meningkat hingga mencapai 2,531 gr/cm3 pada temperatur 950_C selama 2 jam. Hasil pengamatan makro dan mikro yang dilakukan juga menunjukkan bahwa semakin tinggi temperatur sinter dan semakin kecil ukuran butir garam karbonat, maka semakin banyak terbentuk sel pori yang bersifat terbuka.

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Powder metallurgy is one kind of process to produce metal foam that is commonly used to achieve uniformity of pore structure. By using the temperatures 825°C, 850°C, 900°C, 950°C in sintering process and 841 m, 542 m, 420 m particle size of carbonate, have affected the differences in pore structures that are formed in the copper foam at each variabel. The purpose of this research is to control the pore structure in the copper foam that were made by this process by using the variation of sintering temperatures and particle sizes of carbonate.

The results show that the higher the temperature is used in sintering, the copper foam will have lower porosity as 71,748% and higher density as 2,531 gr/cm3 at 950_C for 2 hours. In addition, macrostructure and microstructure observation show that the higher the temperature is used in sintering and the smaller the particle size is used as the filler, the more opened cell pores are formed."

"Latar Belakang: Trauma dentoalveolar merupakan trauma umum terjadi di seluruh dunia yang melibatkan area gigi, tulang alveolar dan jaringan pendukung gigi. Tatalaksana alternatif untuk meregenerasi tulang secara cepat dan aman sehingga dapat berintegrasi baik dengan tubuh adalah dengan prosedur bone grafting. Beberapa jenis bone graft yang tersedia adalah Bio-Oss® dan prototipe karbonat hidroksiapatit. Kedua jenis bone graft memiliki perbedaan pada sumber material. Tujuan: Mengevaluasi perbandingan sitotoksisitas dan mineralisasi sel MC3T3-E1 pada bone graft prototipe karbonat hidroksiapatit dan Bio-Oss®. Metode: Jumlah spesimen yang digunakan dalam penelitian sebanyak 21 spesimen yang terdiri dari uji sitotoksisitas dengan MTT Assay sebanyak 12 spesimen pada 96 well-plate dan uji mineralisasi dengan pewarnaan Alizarin Red sebanyak 9 spesimen pada 24 well-plate. Uji sitotoksisitas dimulai dengan sel MC3T3-E1 dalam medium ekstrak bone graft selama 24 jam. Kemudian, dilanjutkan dengan inkubasi sel dengan larutan MTT selama 2 jam. Uji mineralisasi dilakukan dengan inkubasi sel MC3T3-E1 dalam medium diferensiasi bone graft selama 21 hari. Uji pewarnaan Alizarin Red dilakukan selama 20 menit. Hasil: Berdasarkan uji MTT assay, nilai viabilitas sel pada kedua bone graft yang diuji berada di atas 70% yang dikategorikan tidak sitotoksik berdasarkan acuan SNI ISO 10993-5:2015. Kedua bone bone graft tersebut memiliki perbedaan viabilitas sel secara signifikan (p<0,05). Bone graft prototipe karbonat hidroksiapatit memiliki persentase viabilitas sel lebih tinggi dibandingkan dengan Bio-Oss®. Berdasarkan uji pewarnaan Alizarin Red, bercak kecoklatan yang diperoleh pada spesimen yang tidak terkontaminasi dapat diduga bukan hasil mineralisasi tulang. Kesimpulan: Berdasarkan uji MTT assay, Bone graft prototipe karbonat hidroksiapatit dan Bio-Oss® bersifat tidak sitotoksik. Viabilitas sel pada bone graft prototipe karbonat hidroksiapatit lebih tinggi dari Bio-Oss®. Berdasarkan uji pewarnaan Alizarin Red, nodul mineralisasi tulang melalui pewarnaan Alizarin Red belum berhasil dilakukan sempurna karena adanya kontaminasi. Berdasarkan data spesimen yang tidak terkontaminasi, diperoleh bahwa prototipe karbonat hidroksiapatit dan Bio-Oss® tidak menghasilkan bercak nodul mineralisasi.

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Background: Dentoalveolar trauma is a common global trauma involving the teeth, alveolar bone and supporting tissues of the teeth. An alternative treatment to regenerate bone quickly and safely so that it can integrate well with the body is the bone grafting procedure. Some types of bone graft available are Bio-Oss® and the carbonate hydroxyapatite prototype. The two types of bone graft have differences in material sources. Objective: To evaluate the comparison of cytotoxicity and mineralization of MC3T3-E1 pre-osteoblast cells in carbonate hydroxyapatite prototype and Bio-Oss®. Methods: The number of specimens used in the research was 21 specimens, consisting of 12 specimens cytotoxicity tests with MTT Assay on 96-well plates and 9 specimens mineralization tests with Alizarin Red staining on 24-well plates. The cytotoxicity test was initiated with MC3T3-E1 cells in bone graft extract medium for 24 hours. The cells were then incubated with MTT solution for 2 hours. The mineralization test was carried out by incubating MC3T3-E1 cells in bone graft diferentiation medium for 21 days. The Alizarin Red staining test was performed for 20 minutes. Results: Based on the MTT assay test, the cell viability value in the two bone grafts tested was above 70% which was categorized as non-cytotoxic based on the SNI ISO 10993-5:2015. The two bone grafts showed significant differences in cell viability (p<0.05). The carbonate hydroxyapatite prototype bone graft has a higher percentage of cell viability compared to Bio-Oss®. Based on the Alizarin Red staining test, the brownish spots obtained in the uncontaminated specimens are not considered to be the result of bone mineralization. Conclusion: Based on the MTT assay test, carbonate hydroxyapatite prototype and Bio-Oss® bone grafts are not cytotoxic. Cell viability in the hydroxyapatite carbonate prototype bone graft was higher than Bio-Oss®. Based on the Alizarin Red staining test, bone mineralization nodules using Alizarin Red staining have not been completely successful due to contamination. Based on uncontaminated specimen data, it was found that the hydroxyapatite carbonate prototype and Bio-Oss® did not produce mineralized nodules."