Hasil Pencarian  ::  Simpan CSV :: Kembali

"Lactoferrin is an iron-binding glycoprotein belonging to the transferrin family. It acts as a defense in host animals against microbes and viruses, since it has a broad spectrum of antimicrobial and antiviral activities. This title explores research in the field."

"Penyembuhan luka merupakan sebuah proses transisi yang merupakan salah satu proses paling kompleks dalam fisiologi manusia yang melibatkan serangkaian reaksi dan interaksi kompleks antara sel dan mediator. Fase peradangan bertujuan untuk membuang jaringan mati dan mencegah infeksi. Fase proliferasi bercirikan terbentuknya jaringan granulasi yang disertai kekayaan jaringan pembuluh darah baru, fibroblast, dan makrofag dalam jaringan penyangga yang longgar. Fase kedua yang berlangsung sejak hari ke-8 hingga ke-21 pascaluka merupakan fase terjadinya epitelisasi dan sekaligus memberikan refleksi dalam perawatan luka untuk dapat mencapai kondisi luka yang telah tertutup dengan epitel.Fase terakhir adalah fase maturasi yang bercirikan keseimbangan antara proses pembentukan dan degradasi kolagen. Setidaknya terdapat 3 prasyarat kondisi lokal agar proses penyembuhan luka dapat berlangsung dengan normal, yaitu: 1) semua jaringan di area luka dan sekitarnya harus vital, 2) tidak terdapat benda asing, 3) tidak disertai kontaminasi eksesif atau infeksi. Penulis mengusulkan formulasi pola hirarkis dalam intensi penyembuhan luka yang mengikuti urutan intensi primer sebagai intensi ideal, diikuti intensi tersier, dan yang Vsekunder. Key words: inflammatory mediator, epithelialisation, growth factor, wound healing.

---

Wound healing is a transition of processes which is also recognized as one of the most complex processes in human physiology. Complex series of reactions and interactions among cells and mediators take place in the healing process of wound involving cellular and molecular events. The inflammatory phase is naturally intended to remove devitalized tissue and prevent invasive infection. The proliferative phase is characterized by the formation of granulation tissue within the wound bed, composed of new capillary network, fibroblast, and macrophages in a loose arrangement of supporting structure. This second phase lasts from day 8 to 21 after the injury is also the phase for epithelialisation. The natural period of proliferative phase is a reflection for us in treating wound to reach the goal which ultimately defines as closed wound.

The final maturation phase is also characterized by the balancing between deposition of collagen and its degradation. There are at least three prerequisites which are ideal local conditions for the nature of wound to go on a normal process of healing i.e. 1) all tissue involved in the wound and surrounding should be vital, 2) no foreign bodies in the wound, and 3) free from excessive contamination/infection. The author formulated a step ladder of thinking in regards of healing intentions covering all acute and chronic wounds. Regarding the ?hierarchy? of healing intention, the first and ideal choice to heal wounds is by primary intention followed by tertiary intention and lastly the secondary intention."

"Summary:* In-depth discussion of effective clinical management of oral wound healing * Detailed discussion on processes such as inflammation, reepithelialization and angiogenesis * Written by experts in the field "

"Skripsi ini menjelaskan tentang cerita hidup seorang penyanyi solo yang bernama Kartika Jahja. Dalam cerita hidup nya ia mengalami beberapa peristiwa traumatis yang menciptakan teror dalam dirinya. Musik memiliki fungsi untuk menyampaikan pesan sebagai bentuk penggambaran pengalaman teror yang dialaminya. Selain itu dalam karyanya yang bertransformasi ia juga mengisyaratkan kebangkitan dari teror. Musik dijadikan sebagai sebuah katarsis untuk mengobati luka dalam jiwa dan tubuhnya yang diakibatkan oleh peristiwa traumatis yang menciptakan teror. Teknik partisipan observasi dan wawancara mendalam dilakukan dalam penelitian untuk melihat peristiwa-peristiwa yang terjadi dalam kehidupannya. Melihat narasi-narasi pengalaman hidup berguna untuk mengetahui bagaimana kejadian-kejadian dalam kehidupan musisi mempengaruhi pembentukan karyanya begitu pula sebaliknya.

---

This undergraduate thesis explain about life of a soloist named Kartika Jahja. Along Her life story, she had several traumatic moment that create the “terror”. Music had function to deliver message as a methaphor for her experience of terror that she had. Then, on her transformed music, she tried to tell about her struggle to stand back against terror. Music was made as a catarsist to heal the wound on her body and mind that was created by trauma which in turn shape the terror. Participant observation and in-depth interview was done in this research for viewing events that happens on her life. Viewing this series of events is used for knowing how in her life as a musician some events affects her pieces of an art and vice versa."

"Depresi pasien ulkus diabetikum dapat menurunkan respon imun dan inflamasi yang dibutuhkan pada proses penyembuhan luja. Penelitian kuasi eksperimen ini bertujuan untuk mengidentifikasi pengaruh pengelolaan depresi dengan pranayama terhadap perkembangan proses penyembuhan ulkus diabetikum di RS pemerintah Aceh. Hasil penelitian menunjukkan latihan pranayama dapat mempengaruhi perkembangan proses penyembuhan ulkus dan penurunan skor depresi, namun tidak ditemukan pengaruh pengelolaan depresi dengan pranayama terhadap perkembangan proses penyembuhan ulkus diabetikum.

---

Depression on patient with diabetic ulcer impair immune and inflammation response that are needed in wound healing process. The urpose of this quasi experiment research was to identified the effect of pranayama on patient diabetic ulcer in Aceh government hospital. The result showed that pranayama has positive effect to wound healing progress and to decrease the depression score. But there was no effect of controlling depression by pranayama to wound healing progress."

"Tujuan: Penelitian ini menganalisis hubungan faktor determinan terhadap densitas mandibula dan pengaruhnya terhadap penyembuhan luka ekstraksi. Metode: Penelitian pertama potong-lintang dengan wawancara, pemeriksaan klinis, radiologis dan laboratoris. Penelitian kedua kohort prospektif dengan pemeriksaan klinis dan radiologis. Hasil: Faktor determinan pada densitas mandibula adalah: lama menopause, kadar estradiol, kadar osteoprotegerin, dan polimorfisme gen OPG G1181C. Terdapat perbedaan bermakna penurunan tinggi soket antar densitas mandibula saat awal sampai delapan minggu pasca ekstraksi. Kesimpulan: Model dapat menjelaskan densitas mandibula sebesar 46,90%. Densitas mandibula berpengaruh terhadap penurunan tinggi soket pada penyembuhan luka ekstraksi saat awal sampai delapan minggu pasca ekstraksi.

---

Objective: To analyze the correlation of mandible density determinant factors and its effect in extraction wound healing. Methods: First study was cross-sectional study by interview, clinical, radiology, and laboratory assessment. Second study was prospective cohort by clinical and radiology assessment.

Results: Deteminant factors in mandible density are menopause length, estradiol, osteoprotegerin, and OPG (G1181C) gene polymorphism. There is significant difference between socket height levels in mandible density from the beginning until eight weeks after extraction.

Conclusion: Model described mandible density 46.90%. Mandible density has effect on socket height levels in extraction wound healing from the beginning until eight weeks after extraction."

"

Latar belakang: Autologus growth factor (AGF) merupakan sitokin yang menarik perhatian para ilmuwan di bidang kedokteran dikarenakan memiliki fungsi yang penting dalam memperbaiki dan mempercepat  proses penyembuhan luka. Platelet rich fibrin matrix (PRFM) merupakan generasi terbaru konsentrat trombosit dengan tahapan persiapan yang praktis dan sederhana. Berbagai macam operasi di bidang THT-KL, salah satunya Laringektomi Total (LT). Komplikasi yang sering terjadi adalah terdapatnya fistula faringokutaneus, sehingga diperlukan perhatian dengan seksama terhadap proses penyembuhan luka pasca-LT. Tujuan penelitian: Membuktikan bahwa pemberian PRFM dapat memperbaiki tatalaksana untuk mempercepat penyembuhan pada luka operasi pasca-LT dibandingkan Kelompok kontrol. Metode: Penelitian ini dilakukan pada Divisi Laring Faring THT-KL/ FKUI – RSCM selama Juni – Desember 2019, merupakan penelitian pendahuluan dengan  desain Randomized Control Trial (RCT). Penelitian ini melibatkan 20 pasien dengan karsinoma sel skuamosa (KSS) Laring yang ditatalaksana dengan LT dan dibagi menjadi 10 pasien yang menjalani LT dengan augmentasi menggunakan autologus PRFM intra operasi dan 10 pasien sebagai kontrol. Proses penyembuhan luka diobservasi hingga 2 minggu pascaoperasi. Hasil: Telah dilakukan analisis bivariat dengan uji chi-square, didapatkan perbedaan yang signifikan pada ambang nyeri, edema dan dehisence pada luka stoma (p<0.001), keberhasilan tes minum yang dilakukan pada hari kelima (p<0.001) dan terbentuknya early fistula faringokutan (p=0.03) pada luka pascaoperasi kelompok subjek dengan PRFM dibandingkan tanpa PRFM. Kesimpulan: PRFM terbukti dapat mempercepat penyembuhan luka pasca-LT. Tes minum dapat dilakukan pada hari kelima pada seluruh kelompok subjek dengan PRFM dan menjadikan masa perawatan menjadi lebih singkat. Angka kejadian fistula juga ditemukan sangat berkurang sehingga tatalaksana kemoradiasi tidak tertunda.

Kata kunci: PRFM, Laringektomi total, Fistula faringkutaneus

---

Background: Autologous growth factor (AGF) is a cytokine that attracts the attention of scientists, because of its beneficial to improve and accelerate process of wound healing. Platelet rich fibrin matrix (PRFM) is the latest generation of thrombocyte concentrate with simple preparation. Various kinds of operations in Otolaryngology, for example Total Laryngectomy (TL), a common complication is the presence of pharyngocutaneus fistula, so needed truly attention for wound healing process after TL.  Objective: Proving that administration of PRFM can improve management to accelerate surgical wound healing after TL compared without PRFM. Method: This research was performed in Larynx Pharynx Division of ENT Department FKUI-RSCM from June – Desember 2019. This study is preliminary study using Randomized Control Trial (RCT). There were 20 patients with Laryngeal squamous cell carcinoma treated with TL. Subjecst divided into 10 patientsunderwent TL with autologus PRFM augmentation intra operation and 10 more patients as a control group, then observed two weeks after surgery. Results: Bivariate analysis was performed with chi-square test, showed significant differences in the pain threshold, edema, presence of dehisence in stoma wounds (p<0.001), success of the drinking test conducted on the fifth day (p<0.001) and formation of pharyngocutaneous early fistule (P:0.03) in postoperative wounds between groups of patients that given PRFM and without PRFM. Conclusion: PRFM is proven to accelerate post-operative wound healing after TL. Drinking test can be performed on the fifth day in all subjects of PRFM groups so that time of hospitalized becomes shorter. Incidence rate of fistule is more decreased so that no delayed of chemoradiation.

"

"ABSTRAKLatar Belakang: Produk biologi yang merupakan rekayasa jaringan dapat membantu mempercepat proses penyembuhan luka. Salah satunya yang saat ini banyak digunakan dalam proses penyembuhan luka adalah Platelet Rich Plasma (PRP). Meskipun demikian PRP tidak memberikan hasil optimal, karena berbentuk cairan dan proses pembuatannya membutuhkan bovine thrombin yang bersifat xenologus. Selain itu sebagian besar faktor pertumbuhan telah dilepaskan saat awal aplikasi di luka. Produk biologi lainnya adalah Platelet Rich Fibrin Matrix (PRFM), yang merupakan generasi terbaru konsentrat trombosit yang menghasilkan fibrin alami. Pada operasi THT-KL khususnya plastik rekonstruksi, tandur kulit banyak digunakan untuk defek yang tidak dapat ditutup primer dengan jabir lokal. Dengan cara tersebut penyembuhan luka tandur kulit berlangsung lama, tandur kulit kontraktur dan hasilnya tidak optimal. Aplikasi PRFM pada implantasi tandur kulit diharapkan dapat meningkatkan mutu kesintasan tandur. Walaupun mekanisme kerja PRFM dalam mempercepat proses penyembuhan luka tandur kulit belum diketahui, secara in vitro mengarah pada adanya peran faktor pertumbuhan.Tujuan: Mendapatkan PRFM yang lebih baik dari PRP dalam mempercepat penyembuhan luka tandur kulit, tanpa menggunakan perangkat komersial, serta mengetahui peran faktor pertumbuhan dalam mempercepat penyembuhan luka tandur in vivo.Metode: Sebanyak 150 jaringan biopsi tandur kulit full thickness skin graft (FTSG) dan split thickness skin graft (STSG) yang diperoleh dari 5 ekor babi, dibagi menjadi tiga perlakuan yaitu tandur-kontrol, tandur-PRP, dan tandur-PRFM. Biopsi jaringan tandur dilakukan pada hari ke-1, -3, -7, -14 dan ke-30 sesuai fase penyembuhan luka. Jaringan biopsi dievaluasi secara histologi dengan pewarnaan hematoksilin eosin, trichrome Masson dan Picrosirius red; juga kadar TGFβ1 dan PDGF. Pemeriksaan makroskopik terhadap luka tandur kulit dilakukan dengan mengevaluasi hasil dokumentasi fotografi menggunakan program ImageJ. Pada awal penelitian diukur kadar faktor pertumbuhan TGFβ1 dan PDGF di PRP dan PRFM, serta dinilai karakteristik struktur mikroskopik, ukuran serat fibrin dan diameter trombosit PRFM menggunakan SEM. PRFM dibuat dari PRP tanpa menggunakan perangkat komersial.Hasil: Kadar faktor pertumbuhan, kepadatan sel PMN, sel makrofag, fibroblas, kepadatan kolagen tipe 1 dan kesintasan tandur-PRFM lebih baik dibandingkan tandur-PRP dan tandurkontrol. Diperoleh PRFM babi dan manusia yang memiliki struktur mikrokopik serat fibrin seperti jala dengan trombosit tersebar di serat fibrin. Karakter tersebut menyerupai PRFM yang diperoleh menggunakan perangkat komersial.Simpulan: Pemberian PRFM sebagai preparat trombosit autologus meningkatkan percepatan penyembuhan luka tandur kulit karena mengandung faktor pertumbuhan yang diperlukan pada penyembuhan luka. PRFM dapat dibuat tanpa perangkat komersial.

---

ABSTRACT

Background: Biological products that are tissue engineered can help accelerate the wound healing process. One of the mostly used biological products for wound healing process is Platelet Rich Plasma (PRP). However, it has not provided optimal results, because of its liquid form, the development process that use xenologous bovine thrombin, and most of the growth factors will be released prior to its application on a wound. Another biological product is Platelet Rich Fibrin Matrix (PRFM), a new generation of concentrated blood platelets that produce natural fibrin. For facial plastic and reconstructive surgery, skin graft is often used on defects that cannot be covered primarily by local flap. By this method the wound healing of skin graft is slow, skin graft contracture occured and the results were not optimal. Application of PRFM in the skin graft implants is expected to increase the survival of the graft. Furthermore the mechanism of PRFM in accelerating wound healing process of skin graft is still unknown; reported in vitro studies showed the important role of growth factor.

Objective: To obtain PRFM that is better then PRP in accelerating the healing process of skin graft wound, without using comersial devices. Also to investigate the role of growth factors in accelerating the healing process of skin graft wound by in vivo study.

Methods:. 150 tissue biopsies of full thickness (FTSG) and split thickness (STSG) skin grafts were obtained from 5 porcines with three different treatments, control-graft, PRP-graft and PRFM-graft. Biopsy of each tissue-graft was done on day-1, -3, -7, -14 and -30 according to the phases of wound healing. Each tissue biopsy was evaluated by histopathology using hematoxyilin eosin, trichrome Masson and Picrosirius red stainings; measurement of the TGFβ1 and the PDGF levels was done by ELISA. Macroscopic examination towards skin graft wound was done by evaluating photographic documentation results using ImageJ program. The amount of TGFβ1 and PDGF in PRP and PRFM was determined at the beginning of this research, as well as evaluation of the microscopic structure characteristic, fibrin fiber size and platelets diameter in PRFM by using SEM.

Results: TGFβ1 and PDGF levels, PMN cell, macrophage, fibroblast, and collagen type 1 density, as well as survival graft of PRFM addition were better compared to PRP and control. Porcine and human PRFM has microscopic fibrin fiber structure like nets with the platelets spread on fibrin fibers. This character of the PRFM is similar to the PRFM prepared by using a commercial device.

Conclusion: Application of PRFM as an autologous thrombocyte preparation increase the acceleration of skin graft wound healing because it contains growth factors that are needed in wound healing."

"ABSTRAKLuka kaki diabetik (LKD) merupakan komplikasi kronik diabetes yang meningkatkan mortalitas danmorbiditas, serta menurunkan kualitas hidup. Komplikasi makro dan mikrovaskular/mikrosirkulasimempunyai pengaruh besar terhadap kejadian LKD dan proses penyembuhannya. Kondisimikrosirkulasi dapat dinilai melalui pemeriksaan transcutaneous perfusion oxygen (TcPO2). Kondisimikrosirkulasi dipengaruhi oleh HbA1c, glukosa darah sewaktu, neuropati, fibrinogen, PAI-1,hsCRP, indeks MMP-9, indeks TcPO2, dan indeks TcPCO2, yang akan memengaruhi terbentuknyajaringan granulasi.Penelitian ini bertujuan untuk mengetahui peran HbA1c, GDS, neuropati, fibrinogen, PAI-1, hsCRP,indeks MMP-9, terhadap indeks TcPO2, indeks TcPCO2, dan indeks granulasi, serta mengetahuiperan serta indeks TcPO2 dan indeks TcPCO2 terhadap indeks granulasi pada luka kaki diabetik.Sebanyak 68 subjek LKD tanpa penyakit arteri perifer di RS dr. Cipto Mangukusumo dan beberaparumah sakit jejaring, pada Desember 2015?Desember 2016, diberikan perawatan standar dandipantau setiap minggu sebanyak 4 kali. Pada pemantauan ke-1, ke-2, dan ke-3, dilakukandokumentasi LKD, pengambilan darah vena sebanyak 7,7 mL untuk pemeriksaan fibrinogen, PAI-1,hsCRP, MMP-9, dan TIMP-1, darah arteri sebanyak 2 mL untuk pemeriksaan analisis gas darah,serta pemeriksaan TcPO2 dan TcPCO2 dengan menggunakan TCM TOSCA/CombiM monitoringsystems buatan Radiometer. Pada pemantauan ke-4, hanya dilakukan dokumentasi LKD.Pengukuran luas luka dan jaringan granulasi dinilai berdasarkan hasil dokumentasi fotografi denganmenggunakan program ImageJ. Penilaian neuropati menggunakan pemeriksaan interval RR dankecepatan hantar saraf. Data laboratorium lainnya diperoleh dari data sekunder rekam medis.Kemudian dilakukan analisis data dengan menggunakan path analysis (analisis lajur) pada datarepetitif dan SPSS pada data nonrepetitif.Berdasarkan analisis didapatkan hubungan antara peningkatan glukosa darah sewaktu, fibrinogen,dan PAI-1 dengan penurunan indeks TcPO2. Didapatkan juga hubungan antara beratnya neuropatimotorik dan sensorik, peningkatan glukosa darah sewaktu, fibrinogen, PAI-1, dan hsCRP denganpenurunan indeks granulasi. Tetapi, indeks granulasi tidak dipengaruhi oleh indeks TcPO2. IndeksTcPCO2 tidak memiliki hubungan terhadap semua variabel tersebut, kecuali hsCRP dan indeksTcPCO2 tidak memengaruhi indeks granulasi.Indeks TcPO2 pada LKD dipengaruhi oleh kadar glukosa darah sewaktu, fibrinogen, dan PAI-1,tetapi tidak memengaruhi tumbuhnya jaringan granulasi. Tumbuhnya jaringan granulasi dipengaruhioleh glukosa darah sewaktu, neuropati motorik dan sensorik, peningkatan kadar fibrinogen, PAI-1,dan hsCRP. Selain itu, indeks TcPCO2 tidak memengaruhi indeks granulasi

---

ABSTRACTDiabetic foot wounds/ulcer (DFU) is chronic complication of diabetes, which increasesmortality and morbidity, and lower quality of life. Macro and microvascular/microcirculationcomplications has a great influence on DFU and healing process. Microcirculation condition canbe seen from transcutaneous perfusion oxygen (TcPO2). The growth of granulation tissue in thehealing process is determined by microcirculation condition, among others influenced byHbA1c, random blood glucose, neuropathy, fibrinogen, PAI-1, hsCRP, MMP-9 index, TcPO2index, and TcPCO2 index.This study aimed to investigatethe role of HbA1c, random blood glucose, sensory, motoric, andautonomy neuropathy, fibrinogen, PAI-1, hsCRP, MMP-9 index, TcPO2 index, TcPCO2 index,and granulation index, as well as the relationship between TcPO2 index, TcPCO2 index andgranulation index in diabetic foot wounds.As much as 68 subjects DFU without peripheral arterial disease, in Cipto MangunkusumoReferral National Hospital, on December 2015?December 2016, were given standardmanagementof diabetic foot ulcer and monitored once a week for four times. In the 1st, 2nd, and3rd monitoring, DFU was documented, then 7.7 mL of venous blood was taken for fibrinogen,PAI-1, hsCRP, MMP-9, and TIMP-1 examination, also 2 mL arterial blood for blood gasanalysis, and then examination of TcPO2 and TcPCO2was performed using TCM4TOSCA/CombiM monitoring systems made by Radiometer. In the 4th monitoring, only DFUwas documented. Wound and granulation size was measured through photographicdocumentation using ImageJ program. Neuropathy was diagnosed based on RR interval andnerve conduction velocity study. Other laboratory data were obtained from medical records. Thedata were analysed by path analysis for repetititive data and SPSS for nonrepetitive data.From analysis, there is a significant correlation between the increasing random blood glucose(RBG), fibrinogen, and PAI-1 with the decreasing of TcPO2, also found a significantrelationship between the severity of sensory and motoric neuropathy, the increasing levels ofRBG, fibrinogen, PAI-1, and hsCRP with the decreasing of granulation index. But, TcPO2 indexdoes not influence granulation index. TcPCO2 index does not have significant correlation withall these variables, except hsCRP. Moreover, TcPCO2 index also does not influence granulationindex.TcPO2 index of DFU is affected by RBG, fibrinogen, PAI-1, but does not affect the growth ofgranulation tissue. Granulation tissue?s growing is influenced by the sensory and motoricneuropathy, increased levels of fibrinogen, PAI-1, and hsCRP. Furthermore, TcPCO2 index doesnot influence granulation?s growth."

"Wound healing is a biological response that occurs following a trauma or pathologic condition of the oral mucosa. Wounds require proper management so they may heal faster and without complication. The number of fibroblast cells seen in the oral mucosa is a strong indicator of wound healing. In the remodelling phase of wound healing, fibroblast cell proliferation decreases as collagen fibres are synthesized. Noni leaf (Morinda citrifolia L.), a part of the noni plant, is traditionally used to heal soft tissue wounds in Indonesia. The leaves have potential chemical compounds that may be useful in the wound repair process. Objective: This paper aims to examine the effect of ethanol based pastes of the Indonesian noni leaf on oral mucosa wound healing by investigating visual wound closure and fibroblast cell counts in Wistar rats. Methods: Rats were divided into 2 control groups and 4 treatment groups. The pastes were formulated in concentrations of 2.5%, 5%, 10%, and 20% and were applied onto wounds of the oral mucosa of the rats. One control group was given no medication and the other control group had an ethanol gel with a concentration of 10% noni leaf extract applied to their intraoral wounds. Result: Result revealed that all groups treated with noni leaf paste experienced better wound closure (p<0.05) when compared to the control groups. Fibroblast cell counts showed little significance amongst all groups (p=0.143), however fibroblast cell counts of groups treated with noni leaf paste, of all concentrations, were lower than both control groups. Conclusion: These results suggest that topical application of noni leaf paste promotes better oral mucosa wound healing than gel formulas but shows no significant difference in the amounts of fibroblasts during the remodelling phase."