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"[ABSTRAKLatar belakang: Neoplasma sel plasma (NSP) adalah proliferasi sel plasma neoplastik yang tumbuh soliter menjadi plasmasitoma tulang soliter (PTS) dan plasmasitoma ekstrameduler (PEM) serta multipel (MM)). Saat ini perjalanan penyakit dari plasmasitoma menjadi MM sulit diprediksi. Bartl mengklasifikasikan derajat keganasan berdasarkan histomorfologik menjadi rendah, sedang dan tinggi. Penelitian ini bertujuan menggunakan klasifikasi Bartl untuk menilai perjalanan PTS dan PEM menjadi MM dihubungkan dengan ekspresi TP53 dan Ki-67.Bahan dan cara: Pada 32 kasus NSP yang berasal dari PTS 14 kasus, PEM 5 kasus, maupun MM sebanyak 13 kasus, diklasifikasikan menjadi 3 kelompok derajat keganasan menurut Bartl yaitu derajat keganasan ringan, sedang dan tinggi. Selanjutnya dilakukan pulasan IHK TP53 dan Ki-67 pada seluruh kasus dan dihitung persentase positifitas.Hasil: Berdasarkan derajat keganasan, derajat rendah ditemukan pada 10 (31,2%) MM, derajat sedang pada 5 (15,6%) PTS dan derajat tinggi pada 2 (6,2%) PTS dan PEM. Peningkatan ekspresi TP53 ditemukan pada derajat Bartl yaitu median derajat rendah 4%, derajat sedang 16%, dan derajat tinggi 10%. Terdapat perbedaan ekspresi TP53 yang bermakna antara derajat keganasan rendah dan sedang (p=0,004). Rerata indeks proliferasi Ki-67 pada derajat keganasan rendah 57%, derajat sedang 44,6%, dan derajat tinggi 32,6%. Tidak ditemukan perbedaan antara indeks proliferasi Ki-67 dengan derajat keganasan menurut Bartl (p=0,339). Tidak terdapat hubungan antara ekspresi TP53, Ki-67 dengan usia. Kesimpulan: Peningkatan ekspresi TP53 pada NSP sejalan dengan peningkatan derajat keganasan Bartl, terutama pada derajat rendah dan sedang. Klasifikasi Bartl ditambah dengan pulasan TP53 saja tidak cukup untuk memprediksi perkembangan PTS dan PEM menuju MM.

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ABSTRACTBackground: Plasma cell neoplasm (PCN) is a neoplastic plasma cells proliferation including solitary bone plasmacytoma (SBP) and extramedullary plasmacytoma (EMP) and multiple myeloma (MM). Until now the development of disease to MM is unpredictable. Bartl classifies the degrees of malignancy histomorphologically as low, intermediate and high. This research aims using Bartl's classification and expression of TP53 and Ki-67 to assess the development of SBP and EMP to MM.Materials and methods: In 32 cases of PCN derived from 14 cases of SBP, 5 cases of EMP, and 13 MM case, then classified into 3 groups based on Bartl's degrees of malignancy as low, intermediate, and high. Furthermore all cases stained by IHC TP53 and Ki-67 and evaluated the percentage of positivity. Results: Bartl's low degree was found in 10 (31,2%) MM case, intermediate in 5 (15,6%) SBP and high in 2 (6,2%) SBP and EMP. TP53 expression, obtainable at 4% of low, 16% of intermediate, and 10% of high degree. There is significant difference between TP53 expression in low and intermediate degree (p = 0,004). Mean proliferation index of Ki-67 is 57% in low, 44,6% in intermediate and 32,6% in high degree. There is no significant difference of Ki-67 proliferation indexes among the group (p = 0,339). There is no correlation between expressions TP53, Ki-67 and age.Conclusion: Increasing expression TP53 is in line with Bartl's degrees of malignancy, especially on low and inter.;Background: Plasma cell neoplasm (PCN) is a neoplastic plasma cells proliferation including solitary bone plasmacytoma (SBP) and extramedullary plasmacytoma (EMP) and multiple myeloma (MM). Until now the development of disease to MM is unpredictable. Bartl classifies the degrees of malignancy histomorphologically as low, intermediate and high. This research aims using Bartl?s classification and expression of TP53 and Ki-67 to assess the development of SBP and EMP to MM.Materials and methods: In 32 cases of PCN derived from 14 cases of SBP, 5 cases of EMP, and 13 MM case, then classified into 3 groups based on Bartl?s degrees of malignancy as low, intermediate, and high. Furthermore all cases stained by IHC TP53 and Ki-67 and evaluated the percentage of positivity. Results: Bartl?s low degree was found in 10 (31,2%) MM case, intermediate in 5 (15,6%) SBP and high in 2 (6,2%) SBP and EMP. TP53 expression, obtainable at 4% of low, 16% of intermediate, and 10% of high degree. There is significant difference between TP53 expression in low and intermediate degree (p = 0,004). Mean proliferation index of Ki-67 is 57% in low, 44,6% in intermediate and 32,6% in high degree. There is no significant difference of Ki-67 proliferation indexes among the group (p = 0,339). There is no correlation between expressions TP53, Ki-67 and age.Conclusion: Increasing expression TP53 is in line with Bartl?s degrees of malignancy, especially on low and inter, Background: Plasma cell neoplasm (PCN) is a neoplastic plasma cells proliferation including solitary bone plasmacytoma (SBP) and extramedullary plasmacytoma (EMP) and multiple myeloma (MM). Until now the development of disease to MM is unpredictable. Bartl classifies the degrees of malignancy histomorphologically as low, intermediate and high. This research aims using Bartl’s classification and expression of TP53 and Ki-67 to assess the development of SBP and EMP to MM.Materials and methods: In 32 cases of PCN derived from 14 cases of SBP, 5 cases of EMP, and 13 MM case, then classified into 3 groups based on Bartl’s degrees of malignancy as low, intermediate, and high. Furthermore all cases stained by IHC TP53 and Ki-67 and evaluated the percentage of positivity. Results: Bartl’s low degree was found in 10 (31,2%) MM case, intermediate in 5 (15,6%) SBP and high in 2 (6,2%) SBP and EMP. TP53 expression, obtainable at 4% of low, 16% of intermediate, and 10% of high degree. There is significant difference between TP53 expression in low and intermediate degree (p = 0,004). Mean proliferation index of Ki-67 is 57% in low, 44,6% in intermediate and 32,6% in high degree. There is no significant difference of Ki-67 proliferation indexes among the group (p = 0,339). There is no correlation between expressions TP53, Ki-67 and age.Conclusion: Increasing expression TP53 is in line with Bartl’s degrees of malignancy, especially on low and inter]"

"Latar belakang: Platelet rich plasma (PRP) merupakan faktor pertumbuhan yang mendukung proliferasi, diferensiasi sel punca in vitro. PRP diyakini dapat digunakan sebagai alternatif pengganti dari fetal bovine serum (FBS) karena bersifat xenofree. Tujuan dari penelitian ini adalah untuk mengetahui efisiensi PRP dalam mendukung proliferasi dan diferensiasi SSCs dan menganalisis korelasi antara tingkat spermatogenesis melalui penilaian Johnson dengan ekspresi gen potensi proliferasi (PLZF, OCT4) dan diferensiasi (CKIT) SSCs.Metode: SSCs diisolasi dari tiga sisa jaringan biopsi testis hasil ektraksi spermatozoa (TESA/TESE) dari pasien azoospermia. Hasil isolasi sel dikultur pada medium DMEM-F12 dengan faktor pertumbuhan spesifik (GDNF, bFGF, EGF) yang selanjutnya dibedakan menjadi dua kelompok medium kultur berdasarkan penambahan PRP atau FBS. Hasil sel kultur dianalisis ekspresinya terhadap gen PLZF, OCT4, dan CKIT dengan qRT-PCR. Tingkat spermatogenesis dianalisis dengan penilaian Johnson melalui pemeriksaan histologi.Hasil: PLZF, OCT4, dan CKIT diekspresikan oleh hasil sel kultur pada kelompok PRP dan FBS, namun tidak bermakna signifikan. Tidak terdapat korelasi antara tingkat spermatogenesis dengan ekspresi gen potensi proliferasi (PLZF dan OCT4) dan diferensiasi (CKIT) SSCs pada kelompok PRP dan FBS.Kesimpulan: PRP mampu mendukung potensi proliferasi dan diferensiasi SSCs in vitro serta dapat menjadi alternatif pengganti FBS.

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Background: Platelet rich plasma (PRP), performing as an alternative candidate to fetal bovine serum (FBS), is a concentrate containing growth factors, support proliferation and differentiation of stem cells in vitro. The objective of this work was to determine the efficiency of PRP in supporting SSCs proliferation and differentiation and assessed the correlation between the level of spermatogenesis through scoring Johnson toward the proliferation and differentiation of SSCs in vitro.

Methods: SSCs were isolated from three of surplus testicular tissue by sperm extraction (TESA/TESE) from azoospermic patients, then SSCs were cultured into DMEM-F12 with growth factors (GDNF, bFGF, EGF), further categorized into PRP and FBS groups. The resulting cell was quantitative analyzed by qRT-PCR towards the expression of PLZF, OCT4 and CKIT. The level of spermatogenesis was observed by scoring Johnson from histology measurement.

Results: The qRT-PCR analysis revealed the expression of PLZF, OCT4 and CKIT in the resulting cell culture. The difference was statistically insignificant among PRP and FBS. There was no correlation between the potency of proliferation (PLZF and OCT4) and differentiation (CKIT) of SSCs toward the level of spermatogenesis in both groups.

Conclusion: PRP could support the maintenance of proliferation and differentiation SSCs in vitro and could be developed as an alternative supplementation of FBS."

"Latar Belakang: pulpa memiliki sifat low-compliance yang memengaruhi proses regenerasinya. Tujuan: menganalisis potensi Platelet- Rich Plasma (PRP) Eksosom terhadap regenerasi pulpa gigi secara in-vitro viabilitas sel, aktivitas migrasi, dan ekspresi Vascular Endothelial Growth Factor-A (VEGF-A) hDPSCs. Metodologi: hDPSC sembilan gigi molar tiga dikultur dengan metode enzyme digestion (ED) yang dipanen pada P3 dan P4. Kemudian dikultur di dalam enam media, yaitu: Dulbecco's Modified Eagle Medium (DMEM) dan 10% PRP sebagai kelompok kontrol, dan 0,5%, 1%, dan 5% eksosom dari PRP. Semua kelompok memiliki tiga rangkap biologis (Triplo). Uji viabilitas sel dievaluasi dengan MTT assay, aktivitas migrasi sel dengan Scratch Assay dan Transwell Migration Assay, dan ekspresi VEGF-A dengan Enzyme-Linked Lmmunosorbent Assay (ELISA). Analisis data dilakukan dengan uji One Way ANOVA (p <0,05) serta uji Kruskal-Wallis dan post hoc Mann-Whitney (p <0,05). Hasil: viabilitas hDPSCs tertinggi pada 24, 48 dan 72 jam observasi pada kelompok Eksosom dari PRP 5% (p <0,05). Eksosom dari PRP 5% menunjukkan aktivitas migrasi yang lebih tinggi dibandingkan dengan kelompok lain, meskipun terdapat perbedaan tidak bermakna dengan kontrol PRP 10% (p >0,05). Ekspresi VEGF-A hDPSCs tertinggi terdapat pada kelompok PRP Eksosom 5% pada 72 jam observasi. Kesimpulan: eksosom dari PRP 5% berpotensi menginduksi regenerasi pupa gigi manusia.

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Background: pulp has low-compliance properties that affect its regeneration process. Objective: to analyze the potential of Platelet-Rich Plasma (PRP) exosomes on the regeneration of dental pulp by in-vitro evaluation of cell viability, migration activity, and expression of Vascular Endothelial Growth Factor-A (VEGF-A) hDPSCs. Methodology: hDPSCs of nine third molars cultured by enzyme digestion (ED) method were harvested at P3 and P4. Then cultured in six media, Dulbecco's Modified Eagle Medium (DMEM) and 10% PRP as a control group, and 0.5%, 1%, and 5% exosomes of PRP. All groups had a biological triple (Triplo). Cell viability assay was evaluated by MTT assay, cell migration activity by Scratch Assay and Transwell Migration Assay, and VEGF-A expression by Enzyme-Linked Lmmunosorbent Assay (ELISA). Data analysis was performed using One Way ANOVA (p < 0.05) and Kruskal-Wallis and post hoc Mann-Whitney tests (p < 0.05). Results: The viability of hDPSCs was highest at 24, 48 and 72 hours of observation in the Exosomes group of 5% PRP (p < 0.05). Exosomes from 5% PRP showed higher migratory activity compared to other groups, although there was no significant difference with 10% PRP control (p > 0.05). The highest expression of VEGF-A hDPSCs was found in the 5% PRP Exosomes group at 72 hours of observation. Conclusion: exosomes of 5% PRP have the potential to induce the regeneration of human dental pulp."

"This study was designed to optimalize the use of opididymal or ejaculate sperm and plasma for in vitro fertilization,that sperm agglutination was found at preparation. The rate of sperm agglutination was calculated the head-to-head sperm agglutination that were incubated in KR-HEPES medium in 38.5 oC with 5% CO2 at 1,3,5 and 7 hours culture in vitro....."

"In this paper ,the growth of GaN:Mn thin films by plasma-assisted metalorganic chemical vapor deposition (PAMOCVD) method is reported ..."

"Pendahuluan : Giant Cell Tumor tulang (GCT) merupakan tumor tulang jinak yang dapat secara lokal bersifat agresif dengan tingkat rekurensi mencapai 20%. Antigen Ki-67 dan p53 adalah penanda imunohistokimia pada GCT yang menandakan proliferasi sel dan supresi tumor. Penelitian ini menganalisis hubungan antara penanda Ki-67 dan p53 dengan rekurensi pada kasus GCT.Metode : Penelitian adalah suatu studi Cross-sectional kategorikal. Data yang dikumpulkan adalah data demografis pasien, keterangan terkait diagnosis dan tindakan serta hasil pemeriksaan Ki-67 dan p53. Data pasien Ekspresi Ki-67 dan p53 dievaluasi dengan teknik pewarnaan imunohistokimia menggunakan metode avidin-biotin complex perioxidase dengan menggunakan kit LSAB2.Hasil : Terdapat 26 laki-laki dan 37 perempuan dengan usia rata-rata adalah 34,77 tahun berkisar antara 16 sampai 61 tahun. 13 kasus dengan rekurensi lokal. Tidak terdapat hubungan antara rekurensi dengan karakteristik tumor (jenis kelamin, usia, ukuran tumor, lokasi tumor, stadium tumor dan tindakan operasi). Tidak ada hubungan antara Ki-67 (p=0.524) dan rekurensi lokal serta terdapat hubungan yang signifikan antara p53 dengan rekurensi lokal (p=0.048).Kesimpulan : Ekspresi Ki-67 tidak berhubungan dengan rekurensi, sedangkan ekspresi p53 berhubungan dengan rekurensi giant cell tumor tulang. Tidak terdapat hubungan antara rekurensi lokal dengan karakteristik tumor (jenis kelamin, usia, lokasi tumor, ukuran tumor, stadium tumor dan tindakan operasi).

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Introduction : Giant cell tumor of bone (GCTB) is a benign neoplasm that may be locally aggressive with recurrence rate reaching 20%. Ki-67 and p53 are immunochemistry markers that marked cell proliferations and tumor suppression. This research analyze the association between Ki-67 and p53 with recurrence of GCT.Method :This study is a Cross-sectional categorical study. Demography of the patients, diagnosis and treatment related to the GCT, and Ki-67 and p53 results were taken. The expression of Ki-67 and p53 were evaluated using a immunochemistry staining with avidin-biotin complex peroxidase by using KSAB2 kit.Result : There are 26 men and 37 women with an average age is 34.77 years ranged from 16 to 61 years. 13 cases with local recurrence. There is no association between recurrence and tumor characteristics (sex, age, tumor size, tumor location, stage and operation). There is no association between Ki-67 with local recurrence (p=0,524) and a significant association between p53 and local recurrence (p=0,048).Conclusion : Ki-67 was not associated with recurrence, mean while p53 was associated with recurrence of GCT. There is no association between recurrence and tumor characteristics (sex, age, tumor size, tumor location, stage, and operation)."

"Xenoprotein yang terkandung dalam medium ekspansi standar yang digunakan untuk kultur sel punca hematopoietik (SPH) CD34+ berisiko menyebabkan graft-versus-host disease pada pasien penerima cangkok SPH CD34+. Diperlukan suplementasi medium ekspansi xeno-free untuk menurunkan risiko graft-versus-host disease pada pasien penerima cangkok. Suplementasi medium kultur ekspansi menggunakan platelet-rich plasma (PRP) dan human serum albumin (HSA) yang keduanya berasal dari manusia diharapkan dapat menggantikan suplementasi xenoprotein dalam kultur. Platelet-rich plasma diketahui mampu meningkatkan laju proliferasi sel punca, sementara human serum albumin mampu mempertahankan kepuncaan sel punca lebih baik dari fetal bovine serum. Kombinasi PRP dan HSA sebagai suplementasi medium ekspansi diharapkan mampu meningkatkan proliferasi dan mempertahankan kepuncaan SPH CD34+. Pengaruh kombinasi PRP dan HSA, rasio optimal persentase gradien suplementasi PRP dan HSA, serta durasi optimal kultur yang mampu mendukung proliferasi dan mempertahankan sifat kepuncaan SPH CD34+ perlu diketahui. Jumlah sel hidup dihitung menggunakan metode eksklusi trypan blue untuk melihat kemampuan medium uji dalam mendukung proliferasi. Fenotipe SPH CD34+ dianalisis menggunakan flow cytometry untuk mengetahui kemampuan medium uji dalam mempertahankan kepuncaan. Kombinasi suplementasi PRP dan HSA mampu meningkatkan proliferasi dan mempertahankan kepuncaan hingga hari ke-7. Persentase gradien PRP : HSA terbaik merupakan 3 : 2 berdasarkan kemampuannya dalam meningkatkan proliferasi dan mempertahankan sifat kepuncaan SPH CD34+. Kombinasi PRP dan HSA memiliki efek positif terhadap kultur SPH CD34+

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Xenoprotein contained in CD34+ hematopoietic stem cell standard culture expansion medium has the risk of causing graft-versus-host disease (GVHD) in recipient of CD34+ HSC graft. Xeno-free supplementation in expansion medium is required to reduce the risk of GVHD in graft recipient. Supplementation of expansion medium using platelet-rich plasma (PRP) and human serum albumin (HSA), both originate from humans, hopefully has the ability to replace xenoprotein supplementation in culture. Platelet-rich plasma is known to increase the rate of stem cell proliferation, while human serum albumin is able to maintain stem cell’s stemness better than fetal bovine serum. The combination of PRP and HSA as expansion medium supplementation is expected to increase proliferation and maintain the stemness of CD34+ HSC. The effect of PRP and HAS combination, the optimal ratio of the percentage gradient of PRP and HSA supplementation, as well as the optimal duration of culture that can support proliferation and maintain CD34+ HSC stemness are to be studied. Live cells were counted using the trypan blue exclusion method to see the ability of the test medium to support proliferation. CD34+ HSC phenotype was analyzed using flow cytometry to determine the ability of test medium to maintain stemness. Combination of PRP and HSA supplementation are able to increase proliferation and maintain peaks until the 7th day. The best PRP : HSA gradient percentage is 3 : 2 based on its ability to increase proliferation and maintain SPH CD34+ stem properties. PRP and HSA combination has positive effects on CD34+ HSC culture."

"Diabetes melitus tipe 2 (DMT2) adalah penyakit yang ditandai dengan hiperglikemia dan dikaitkan dengan keadaan inflamasi kronis, Sel punca merupakan terapi sel yang menjanjikan untuk DMT2 namun, efek parakrin dari hasil sekresi sel punca atau sekretom lebih potensial. Sekretom dan Autologous Activated Platelet Rich Plasma (aaPRP) berpotensi sebagai terapi bebas sel dan memiliki kandungan biomolekul yang sama untuk terapi DMT2. Penelitian ini bertujuan untuk mengevaluasi sitokin dan faktor pertumbuhan pada aaPRP dan sekretom sebagai strategi awal pengembangan aaPRP dan sekretom untuk DMT2. Penelitian ini adalah eksperimental in-vitro dengan total 13 pasien (10 DMT2 dan 3 Non DMT2). Analisis total protein dan Luminex terhadap sitokin TNF-α, IL-6, IL-10, growth factor VEGF, dan FGF dilakukan pada plasma, aaPRP, dan sekretom. Sekretom diisolasi dari sel punca jaringan adiposa (ADSC) dengan suplemtasi media kultur PRP Kontrol dan FBS. Hasil penelitian menunjukkan, sitokin dan growth factor dari aaPRP dan sekretom Non DMT2, mayoritas lebih tinggi dibandingkan DMT2. Terdapat perbedaan sitokin dan growth factor antara sekretom dengan media PRP Kontrol dan FBS. Kondisi DMT2 mempengaruhi sitokin dan growth factor pada aaPRP. Sitokin dan growth factor pada sekretom dipengaruhi oleh kondisi DMT2 dan media kultur.

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Type 2 diabetes mellitus (T2DM) is a disease characterized by hyperglycemia and is associated with a chronic inflammatory state. Stem cells are a promising cell therapy for T2DM however, the paracrine effect of stem cell secretions, or the secretome, has more potential. Secretome and Autologous Activated Platelet Rich Plasma (aaPRP) can be cell-free therapies for T2DM. This study aims to evaluate cytokines and growth factors in aaPRP and the secretome as an initial strategy for developing aaPRP and the secretome for T2DM. This was an in-vitro experimental study with 13 patients (10 T2DM and 3 non-T2DM). Total protein and Luminex assays of the cytokines TNF-α, IL-6, IL-10, growth factor VEGF, and FGF were taken from plasma, aaPRP, and the secretome. The secretome was isolated from adipose tissue stem cells (ADSC) by supplementing control PRP and FBS culture media. The results showed that most cytokines and growth factors from aaPRP and the non-DMT2 secretome were higher than those from DMT2. There are differences in cytokines and growth factors between the secretome and control PRP and FBS media. T2DM conditions affect cytokines and growth factors in aaPRP. Cytokines and growth factors in the secretome are influenced by T2DM conditions and culture mediums."

"ABSTRAKTerapi regeneratif menggunakan sel punca hematopoietik (SPH) CD34+ merupakan potensi modalitas yang dapat diterapkan dalam mengatasi masalah penyakit hematologi yang sulit disembuhkan. Namun, kultur in vitro SPH saat ini belum optimal karena adanya reaksi penolakan dari penerima sel hasil kultur tersebut. Fetal bovine serum (FBS) sebagai suplemen medium yang umum digunakan dalam kultur SPH merupakan xeno-protein yang dapat memicu reaksi imun dari penerima prosedur terapi. Penelitian ini bertujuan untuk membandingkan antara penggunaan FBS dengan platelet rich plasma (PRP) yang berasal dari sumber manusia terhadap proliferasi dan kepuncaan SPH CD34+. Penelitian eksperimental dilakukan dengan mengukur beberapa parameter antara lain, perhitungan jumlah sel dengan metode eksklusi tryphan biru, kepuncaan SPH CD34+ dengan menggunakan flow cytometry, serta diferenisasi sel yang dinilai dengan pengamatan sel pada pewarnaan giemsa. Hasil penelitian menunjukkan bahwa suplementasi PRP 15% dapat meningkatkan proliferasi SPH CD34+. Analisis flow cytometry menunjukkan bahwa suplementasi PRP kurang mempertahankan kepuncaan SPH CD34+ dengan penurunan kemurnian CD34+ sebesar 31,7%; 31,7%; 21,7% pada kadar suplementasi PRP 5%, 10%, dan 15%. Gambaran sel mononuklear yang ditemukan pada pewarnaan Giemsa menunjukkan bahwa terjadi diferensiasi sel hematopoietik menjadi sel yang lebih spesifik. Oleh karena itu, dapat disimpulkan bahwa PRP 15% merupakan suplementasi yang yang terbaik dalam memicu proliferasi SPH diantara berbagai konsentrasi yang diuji dalam penelitian ini.

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ABSTRACTRegenerative therapy using CD34+ hematopoietic stem cells (HSC) is a potential modality to overcome hematological diseases that are difficult to cure. However, the current in vitro CD34+ culture is not optimal because of the immunological rejection from the recipient. Fetal bovine serum (FBS) as a medium supplement, which commonly used in HSC culture, is a xeno-protein that can trigger an immune reaction from the recipient of a therapeutic procedure. This study aimed to compare the use of FBS with PRP, which originating from the human sources on the proliferation and the stemness of CD34+ HSC. Experimental research was carried out by measuring several parameters, namely the calculation of the number of cells with the blue trypan exclusion method, the stemness of CD34+ HSC using cytometric flow, and cell differentiation which was assessed by observing cells in Giemsa staining. The results showed that 15% of PRP supplementation could increase the proliferation of CD34+. Flow cytometry analysis showed that each dose of PRP supplementation did not maintain the CD34+ SPH function with CD34+ purity reduction of 31.7%; 31.7%; 21.7% in sequence of PRP5%, 10%, and 15% supplementation. The mononuclear cells which were found in Giemsa staining showed that HSC differentiation occurs into more specific cells. Therefore, it can be concluded that 15% PRP is the best supplement concentration of in SPH proliferation in this experiment."