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"Penelitian dilakukan untuk mengetahui pengaruh pemberian infus serai wangi (Cymbopogon nardus (L.) Rendle.) terhadap gambaran histologi ginjal mencit (Mus musculus L.) jantan galur DDY. Sebanyak 28 ekor mencit dibagi secara acak dalam 4 kelompok yang terdiri atas satu kelompok kontrol (KK) dan tiga kelompok perlakuan (KP1, KP2, dan KP3). Kelompok kontrol diberi akuades dan kelompok perlakuan diberi infus serai wangi dengan dosis 2%, 4%, dan 8% (b/v). Masing-masing kelompok perlakuan terdiri atas 7 ekor mencit. Pemberian bahan uji dilakukan selama 5 hari berturut-turut secara oral. Mencit dikorbankan 2 jam setelah pemberian infus terakhir. Organ ginjal diamati secara makroskopik dan mikroskopik. Sediaan histologi diwarnai dengan pewarnaan Hematoksilin Eosin (HE) dan diamati dengan menggunakan mikroskop. Hasil uji statistik menunjukkan bahwa pemberian infus serai wangi pada dosis 2% dan 4% (b/v) tidak berpengaruh terhadap rata-rata diameter kapsula Bowman, glomerulus, jarak ruang Bowman, serta berat basah organ ginjal, sedangkan pada dosis 8% (b/v) terlihat pengaruh yang nyata terhadap diameter kapsula Bowman dan glomerulus, namun tidak berpengaruh terhadap jarak ruang Bowman.

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This research was designed to study the effect of citronella grass (Cymbopogon nardus (L.) Rendle.) infusion on renal histology of DDY male mice (Mus musculus L.). The animals were randomly devided into four groups, consisted of one control group (KK) and three treatment groups (KP1, KP2, and KP3). Control group were given distilled water and the other groups were given doses of citronella grass infusion 2%, 4%, and 8% (w/v) . Each group consisted of 7 mice. The test materials were administered for 5 consecutive days orally. The mice were sacrified at two hours after the last infusion. Kidneys were observed in macroscopic and microscopic. Histology slides stained with Haematoxyline Eosin (HE) and observed with microscope. Statistic study showed that there was no effect of citronella grass infusion at dose of 2% and 4% (w/v) against the average diameter of Bowman?s capsula, glomeruli, diamater of Bowman?s space, and kidneys weight, but there was a significant effect on the average diameter of Bowman?s capsula and glomeruli at a dose of 8% (w/v)."

"Penelitian mengenai LD50 serai wangi telah dilakukan, namun pengaruh dosis serai wangi yang biasa digunakan dalam pengobatan belum banyak diketahui. Penelitian bertujuan untuk mengetahui pengaruh pemberian infus serai wangi (Cymbopogon nardus (L.) Rendle) terhadap organ hati mencit. Penelitian dilakukan dengan cara memberikan infus serai wangi secara oral terhadap 28 ekor mencit jantan (Mus musculus L.) yang dibagi ke dalam 1 kelompok kontrol dan 3 kelompok perlakuan dengan masing-masing perlakuan terdiri dari 7 ulangan. Kelompok perlakuan masing-masing diberi infus serai wangi dengan dosis 2% (b/v), 4% (b/v), dan 8% (b/v), sedangkan untuk kelompok kontrol diberi akuades. Pemberian infus serai wangi dilakukan selama 5 hari, dan 2 jam setelah pemberian infus terakhir mencit dikorbankan. Organ hati mencit diisolasi untuk dibuat preparat dengan metode parafin dan pewarnaan Haematoksilin Eosin (HE). Pengamatan organ hati dilakukan secara makroskopik dan mikroskopik. Berdasarkan data makroskopik berupa warna organ hati dan berat basah tidak ditemukan adanya perbedaan nyata antara kelompok perlakuan. Data mikroskopik terdiri dari data kuantitatif dan semikuantitatif. Hasil analisis data kuantitatif berupa diameter vena sentralis menunjukkan bahwa tidak ada pengaruh perlakuan diameter vena sentralis antara kelompok perlakuan. Data semikuantitatif berupa derajat kerusakan organ menunjukkan bahwa pada pemberian dosis 8% ditemukan adanya kerusakan ringan berupa penimbunan limfosit dan pembendungan darah pada vena sentralis.

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The research about LD50 of Citronella has been done, but the effect of citronella doses that commonly used as medical treatment not much known yet. The aims of this research were to know the effect of using citronella (Cymbopogon nardus (L.) Rendle) infuse to mice?s liver organ.The research was conducted by giving citronella infuse orally to 28 male mice (Mus musculus L.) which divided into 1 control group and 3 experimental groups with 7 repeated for each group. Tree of experimental groups were given citronella infusion in doses 2% (w/v), 4% (w/v), and 8% (w/v), while the control group was given akuades.Citronellainfusion were given for 5 days, and 2 hours after the last infusion was given mices were sacrificed. Liver organ of mices were isolated and histopathology slides were made with parrafin method and using Haematoksilin Eosin (HE) for staining.

Observation liver organ were done macroscopic and microscopic. Based on macroscopic analysis with organ color and wet weight data showed that the treatment has no significant impact to organ color and wet weight among the groups. Microscopic data which divided to quantitative and semiquantitative data showed that quantitatively the treatment has no effect tovena central diamater among the groups, but semiquantitative data which is damaged degree showed that at the doses 8% that was found mild damaged including lymphocytes stuck and blood dammed in central vein."

"Telah dilakukan penelitian untuk mengetahui pengaruh pemberian infusa daun kluwih terhadap spermatogenesis mencit jantan galur DDY. Sebanyak 24 ekor mencit terbagi kedalam 4 kelompok, yakni kelompok kontrol KK yang diberikan akuades, kelompok perlakuan KP1, KP2, dan KP3 yang diberikan infusa daun kluwih dengan dosis berturut-turut, yaitu 2,5; 5; dan 10 g/kg BB. Infusa daun kluwih diberikan selama 36 hari. Kemudian dilakukan pengukuran terhadap berat basah testis, pengamatan dengan angka penilaian Johnsen, dan pengukuran diameter tubulus seminiferus. Data rerata berat testis pada KK, KP1, KP2, dan KP3 berturut-turut ialah 0,307 0,030 g, 0,268 0,014 g, 0,223 0,016 g, dan 0,239 0,020 g. Data rerata diameter tubulus seminiferus KK 205,17 3,79 ?m, KP1 200,97 4,82 ?m, KP2 203,78 3,96 ?m, dan KP3 189,79 3,82 ?m. Data rerata angka penilaian metode Johnsen pada KK, KP1, KP2, dan KP3 berturut-turut adalah 9,71 0,12 , 9,63 0,08 , 9,38 0,10 , dan 9,34 0,11 . Hasil penelitian menunjukkan bahwa pemberian infusa daun kluwih Artocarpus camansi Blanco berpengaruh terhadap spermatogenesis mencit jantan pada dosis 2,5; 5; dan 10 g/kg BB.

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The research has been done to determine the effect of Kluwih leaf rsquo s infusion intake on spermatogenesis of male mice DDY strain. 24 males mice have divided into 4 experimental group control group which only given aquades and treament group which given infusion with doses 2,5 5 10 g kg bw. Test material administated for 36 consecutive days. Then measured the weight of testis, observations with numerical of Johnsen scores, and the diameter of the tubules seminiferous. Mean of testes weigth KK 0,307 0,030 g, KP1 0,268 0,014 g, KP2 0,223 0,016 g, and KP3 0,239 0,020 g. Mean of diameter of tubules seminferous KK 205,17 3,79 m, KP1 200,97 4,82 m, KP2 203,78 3,96 m, and KP3 189,79 3,82 m. Mean of numerical of Johnsen score KK 9,71 0,12 , KP1 9,63 0,08 , KP2 9,38 0,10 , and KP3 9,34 0,11 . Based on LSD test."

"ABSTRAKTelah dilakukan penelitian tentang pengaruh pemberian madu PS (pollen substitute) terhadap gambaran histologi pulau Langerhans pankreas pada mencit (Mus musculus L.) jantan galur DDY yang diinduksi aloksan. Sebanyak 24 ekor mencit dibagi dalam 4 kelompok 6 ulangan, yaitu: kelompok kontrol normal (KK1), kelompok kontrol diabetes (KK2), kelompok perlakuan pemberian madu PS 10% (KP1), dan kelompok perlakuan pemberian madu PS 20% (KP2). Pencekokan madu PS dilakukan setiap hari selama 14 hari berturut-turut. Pada hari ke-15, mencit dikorbankan, organ pankreas diisolasi dan dibuat sediaan histologi menggunakan metode parafin dengan pewarnaan Hematoksilin Eosin (HE). Sediaan diamati secara mikroskopik menggunakan mikroskop cahaya dan mikroproyektor. Data rerata diameter pulau Langerhans mencit dan jumlah sel β pankreas adalah sebagai berikut: KKI (115,03 ± 4,94) µm, (59,47 ± 2,08); KK2 (51,09 ± 8,39) µm, (15,24 ± 2,54); KP1 (106,70 ± 4,75) µm, (40,89 ± 2,33); KP2 (114,24 ± 10,85) µm, (46,78 ± 3,2). Hasil uji LSD (P < 0,05) menunjukkan terdapat perbedaan nyata antara kelompok perlakuan dengan kelompok kontrol KK2. Hal ini berarti bahwa pemberian madu PS (pollen substitute) memengaruhi rerata diameter pulau Langerhans dan jumlah sel β pankreas pada dosis 10% dan 20%.

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ABSTRACTThe study was undertaken to assess the effect of PS (pollen substitute) honey intake on recovery diameter islet of Langerhans pancreas of alloxan-induced male-DDY mice (Mus musculus L.). Tweenty-four male mice were randomly devided into four groups, consisting of normal control group (KK1), treatment control group (KK2), two treatment groups (KP1 and KP2) which was administered with alloxan and PS honey in concentration of 10% and 20%, respectively. Treatments were carried out orally within 14 consecutive days. The mice were sacrified at day 15 (T15). Histology slides was made with paraffin method and stained with Haematoxyline Eosin (HE) and observed with microscope and microprojector. Mean of diameter of islet of Langerhans and pancreatic cell β number : KKI (115,03 ± 4,94) µm, (59,47 ± 2,08); KK2 (51,09 ±8,39) µm, (15,24 ± 2,54); KP1 (106,70 ± 4,75) µm, (40,89 ± 2,33); KP2 (114,24 ±10,85) µm, (46,78 ± 3,2). Least significant difference (LSD) (P < 0,05%) test showed a significant effect of treatment. The result demonstrated the potential beneficiary effect of PS (pollen substitute) Honey for recovery diameter of islet of Langerhans and pancreatic cell β number in concentration of 10% and 20%."

"Penelitian bertujuan untuk mengetahui pengaruh pemberian ekstrak dimer isoeugenol secara oral terhadap kualitas spermatozoa mencit jantan galur DDY. Kelompok kontrol diberi minyak zaitun. Kelompok perlakuan diberi ekstrak dimer isoeugenol dengan dosis 2,4 mg/kg bb; 4,8 mg/kg bb; 9,6 mg/kg bb; dan 19,2 mg/kg bb. Masing-masing kelompok kontrol dan kelompok perlakuan terdiri atas 5 ekor mencit. Bahan uji diberikan setiap hari selama 36 hari berturut-turut.Hasil penelitian menunjukkan adanya penurunan motilitas spermatozoa dan viabilitas spermatozoa pada dosis 4,8 mg/kg bb; 9,6 mg/kg bb; da 19,2 mg/kg bb, juga konsentrasi spermatozoa pada dosis 9,6 mg/kg bb dan 19,2 mg/kg bb. Kenaikan abnormalitas ditemukan pada dosis 4,8 mg/kg bb; 9,6 mg/kg bb; dan 19,2 mg/kg bb. Hasil tersebut menunjukkan bahwa pemberian ekstrak dimer isoeugenol dapat menurunkan kualitas spermatozoa mencit jantan mulai dosis 4,8 mg/kg bb dan memiliki batas aman penggunaan sampai 2,4 mg/kg bb.

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The research aims to determined the effect of isoeugenol dimer extract administrated orally on the quality of spermatozoa of male mice DDY strain. The control group was given olive oil. The treatment group were given isoeugenol dimer with doses of 2.4 mg/kg bw; 4.8 mg/kg bw; 9.6 mg/kg bw; and 19.2 mg/kg bw. Each control group and treatment group consisted of 5 mices. Test material administrated daily for 14 consecutive days.The results showed that a decreased in sperm motility and viability of spermatozoa at doses 4.8 mg/kg bw; 9.6 mg/kg bw; and 19.2 mg/kg bw, as well as the concetration of spermatozoa at doses 9.6 mg/kg bw and 19.2 mg/kg bw. The increased in spermatozoa abnormality were found at doses 4.8 mg/kg bw; 9.6 mg/kg bw; and 19.2 mg/kg bw. The result indicated that administration of extract dimer isoeugenol can reduce the quality of spermatozoa from male mice stated from doses 4.8 mg/kg bw and had usage threshold up to 2.4 mg/kg bw."

"Penelitian telah dilakukan untuk mengetahui potensi hepatoprotektif madu PS (Pollen Substitute) terhadap gambaran histologis hati mencit (Mus musculus) jantan galur DDY. Dua puluh empat ekor mencit jantan dibagi ke dalam 4 kelompok, yaitu kelompok kontrol normal (KK1) yang diberikan akuades dan minyak kelapa; kelompok kontrol perlakuan (KK2) yang diberikan akuades dan CCl4; serta 2 kelompok perlakuan (KP1 dan KP2) yang diberikan madu PS dosis 0,04 ml/20 g bb dan 0,08 ml/20 g bb selama 14 hari berturut-turut, kemudian diinjeksikan CCl4 2 jam setelah pemberian madu PS terakhir. Organ hati diisolasi 24 jam setelah injeksi CCl4. Hasil uji statistik menunjukkan tidak ada pengaruh pemberian madu PS terhadap berat basah organ hati (KK1 [1,57±0,34] gram, KK2 [1,92±0,21] gram, KP1[1,73±0,34] gram, KP2 [1,75±0,30] gram) dan diameter vena sentralis (KK1 [37,91±2,44] μm, KK2 [73,39±3,06] μm, KP1 [70,03±9,65] μm, KP2 [67,61±6,33] μm), serta terdapat pengaruh pemberian madu PS terhadap warna organ hati, persentase derajat kerusakan lobulus hati (derajat 0, 1, 2, dan 3 KK1 [55,70%; 40,00%; 4,33%; dan 0,00%], KK2 [4,56%; 29,00%; 18,67%; dan 47,78%], KP1 [14,22%; 43,11%; 15,22%; dan 27,44%], KP2 [6,34%; 41,33%; 24,67%; dan 27,67%]), dan gambaran histologis hati. Hasil pengamatan menunjukkan pemberian madu PS dosis 0,04 ml/20 g bb memberikan efek hepatoprotektif yang lebih baik dibandingkan madu PS dosis 0,08 ml/20 g bb terhadap gambaran histologis hati.

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The aim of this study was to know hepatoprotective potency of PS (Pollen Substitute) honey to liver histology of DDY strain male mice (Mus musculus). Twenty four male mice were divided into 4 groups, which were normal control group (KK1) which was given aquadest and palm oil, treatment control group (KK2) which was given aquadest and CCl4, as well as 2 treatment groups (KP1 and KP2) which were given PS honey dose 0,04 ml/20 g bb and 0,08 ml/20 g bb in 14 days. Then KP1 and KP2 were induced by CCl4 2 hours after the last administration of honey PS. The liver then was isolated 24 hours after CCl4 injection. The result of statistic test showed that there were no effects of PS honey administration to liver wet weight (KK1 [1,57±0,34] gram, KK2 [1,92±0,21]gram, KP1[1,73±0,34] gram; KP2 [1,75±0,30] gram) and central vein diameter (KK1 [37,91±2,44] μm, KK2 [73,39±3,06] μm, KP1 [70,03±9,65] μm, KP2 [67,61±6,33] μm), and there were effects of PS honey admisnistration to liver color, percentage of liver lobulus damage level (level 0, 1, 2, and 3 of KK1 [55,70%; 40,00%; 4,33%; and 0,00%], KK2 [4,56%; 29,00%; 18,67%; and 47,78%], KP1 [14,22%; 43,11%; 15,22%; and 27,44%], KP2 [6,34%; 41,33%; 24,67%; and 27,67%]), and liver histology. The result of the observation showed that the hepatoprotective effect of administration of PS honey dose 0,04 ml/20 g bw was better than dose 0,08 ml/20 g to liver histology.;"

"Telah dilakukan penelitian tentang pengaruh pemberian infus simplisia rosella (Hibiscus sabdariffa L.) secara oral terhadap kualitas spermatozoa mencit (Mus musculus L.) jantan galur DDY di Laboratorium Biologi Reproduksi dan Perkembangan Departemen Biologi FMIPA UI pada bulan Juni 2010--Maret 2011. Mencit dikelompokkan menjadi lima kelompok. Kelompok kontrol normal (KK1) diberi akuades selama 14 hari berturut-turut. Kelompok kontrol perlakuan (KK2) diberi akuades secara oral selama 14 hari berturut-turut, serta induksi etanol (dosis 2,8 g/kg bb) pada hari ke 8--14 secara intraperitoneal (i.p). Kelompok perlakuan (KP1, KP2, dan KP3) diberi infus simplisia H. sabdariffa L. secara oral dengan dosis 1,5%; 3%; dan 6% selama 14 hari berturut-turut serta induksi etanol pada hari ke 8--14 secara i.p. Hasil uji anava 1-faktor (P < 0,05) menunjukkan bahwa pemberian infus simplisia H. sabdariffa L. dapat meningkatkan motilitas dan menurunkan abnormalitas spermatozoa pada semua kelompok perlakuan. Peningkatan motilitas dan penurunan abnormalitas spermatozoa terbaik dicapai oleh kelompok perlakuan dosis 3% dengan nilai mendekati kelompok kontrol normal.

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The research on the influence of crude roselle (Hibiscus sabdariffa L.) infusion orally on the quality of spermatozoa DDY strain male mice (Mus musculus L.) was conducted in the Laboratory of Reproductive and Developmental Biology, Department of Biology, Faculty of Mathematics and Sciences University of Indonesia on June 2010-March 2011. Mice were divided into 5 groups. The normal control group (KK1) was given distilled water for 14 consecutive days.

The treatment control group (KK2) were given distilled water orally for 14 consecutive days and induced ethanol (dose 2.8 g/kg bw) on day 8-14 by intraperitoneal (i.p). The treatment groups (KP1, KP2, and KP3) were given a crude H. sabdariffa L. infusion orally with doses of 1.5%, 3% and 6% for 14 consecutive days and induced ethanol on day 8-14 by i.p.

The results of one-way anova (P < 0.05) showed that crude H. sabdariffa L. infusion significantly increases spermatozoa motility and decreases abnormalities in all treatment groups. The best enhancement of increased motility and decreased abnormalities of spermatozoa was achieved by treatment group with dose of 3% H. sabdariffa L., with a value approaching the normal control group."

"Penelitian dilakukan untuk mengetahui potensi nefroprotektif dimer isoeugenol terhadap histologi ginjal mencit jantan galur DDY. Tiga puluh ekor mencit jantan dibagi secara acak dalam enam kelompok, yang terdiri atas kelompok kontrol normal, kelompok kontrol kerusakan, dan kelompok perlakuan. Kelompok kontrol dicekok dengan minyak zaitun selama 7 hari berturut-turut. Kelompok perlakuan dicekok dengan larutan dimer isoeugenol dosis 2, 4, 6, dan 8 mg/kg bb selama 7 hari berturut-turut. Kelompok kontrol kerusakan dan kelompok perlakuan diinjeksi intraperitoneal dengan karbon tetraklorida dua jam setelah pemberian dosis hari ke-7. Ginjal diamati secara makroskopik dan mikroskopik. Uji statistik menunjukkan terdapat pengaruh nefroprotektif dimer isoeugenol terhadap rata-rata diameter kapsula Bowman, diameter glomerulus, dan jumlah tubulus proksimal yang menutup, namun tidak terdapat pengaruh terhadap ratarata diameter ruang Bowman dan berat basah ginjal. Hasil penelitian menunjukkan setiap dosis perlakuan dimer isoeugenol memiliki pengaruh nefroprotektif. Dimer isoeugenol dosis 4 mg/kg bb menunjukkan pengaruh paling baik.

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This research was designed to investigate the nephroprotective potential of isoeugenol dimer on DDY strain male mice kidney histology. The animals were divided randomly into six groups, consisted of normal control group, injury control group, and treatment groups. Control groups were administered olive oil orally for 7 consecutive days. Treatment groups were administered doses of isoeugenol dimer solution 2, 4, 6, and 8 mg/kg w/v orally for 7 consecutive days. Injury control group and treatment groups was induced by intraperitoneal administration of carbon tetrachloride 2 hours after the 7th dose. Kidneys were observed in macroscopic and microscopic.

Statistic study showed that there was a nephroprotective effect of isoeugenol dimer against the average diameter of Bowman's capsule, diameter of glomerulus, and total complete obliteration proximal tubule lumen, but no effect was found on the average diameter Bowman's space and kidney weight. The result showed that each dose of isoeugenol dimer has a nephroprotective effect. Isoeugenol dimer dose 4 mg/kg w/v has the best effect."