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"Essential oil mampu membunuh bakteri penghasil VSCs penyebab bau mulut sehingga penelitian ini ingin menguji efektivitas strip dari edible-film mengandung lima macam essential oil terhadap bau mulut. Penelitian ini adalah uji klinis menggunakan desain cross-over, randomized-double-blind, dan placebo-controlled-trial dengan 15 partisipan laki-laki. Parameter yang diukur adalah tongue coating, skor organoleptik, dan VSCs. Berdasarkan hasil penelitian, strip essential oil tidak efektif menurunkan tongue coating, namun, terbukti efektif menurunkan skor organoleptik hingga 60 menit dan menurunkan VSCs hingga 30 menit. Placebo tidak efektif menurunkan ketiga parameter bau mulut. Oleh karena itu, strip essential oil lebih efektif menurunkan bau mulut dibandingkan dengan placebo.

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Essential oil could kill the VSCs-producing bacteria, so, this research was to determine the efficacy of strip made from edible-film containing five types of essential oil on oral malodor. This research was clinical trial using cross-over, randomized-double-blind, and placebo-controlled-trial design with 15 male participants. The measured parameters were tongue coating, organoleptic score, and VSCs. From the result, essential oil strip couldn`t reduce tongue coating, however, it was significantly reduce organoleptic score until 60 minutes and VSCs until 30 minutes. Placebo couldn`t reduce all three oral malodor parameters. So, essential oil strip is more effective than placebo in reducing oral malodor."

"ABSTRAK Latar Belakang: MTA bersifat biokompatibel dan dapat digunakan untuk perawatan kaping pulpa. Saat ini dikembangkan semen berbasis kalsium silikat sama seperti MTA dengan penambahan steroid, yaitu Odontocem. Tujuan:Membandingkan efek toksisitas odontocem dan MTA-Angelus terhadap viabilitas sel fibroblas. Metode:Sel fibroblast embrio ayam direndam dalam larutan odontocemdan dan MTA-Angeluspada 24 dan 72 jam. Viabilitas sel dihitung menggunakanMTT Assay. Hasil:Pada kelompok odontcemdan MTA-Angelus, terdapat perbedaan bermakna (p≤0,05 ) dibandingkan dengan kontrol. Pada paparan 24 jam, tidak terdapat perbedaan bermakna antara odontocem dengan MTA-Angelus. Kesimpulan:Odontocem dan dan MTA-Angelus menurunkan viabilitas sel pada 24 jam dan meningkatkan pada 72 jam.ABSTRACT Background:MTA is proved tobe biocompatible and can be used for pulp capping treatment.Currently, calcium silicate based cement similar to MTA with steroid,called Odontocem has been developed.Objective:To compare effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability.Method:Fibroblast cells of chicken embryonic were immersed separately in odontocem and MTA-Angelus solution for 24 and 72 hours.Cells viability was analyzed with MTT Assay.Result:There was a significant difference (p>0.05) in Odontocem and MTA-Angelus group compared to control.At the24-hour immersion, there was nosignificant difference between odontocem and MTA-Angelus.Conclusion:Odontocem and MTA-Angelus decreased the viability of fibroblast at 24 hours and increased them at 72 hours.;Background:MTA is proved tobe biocompatible and can be used for pulp capping treatment.Currently, calcium silicate based cement similar to MTA with steroid,called Odontocem has been developed.Objective:To compare effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability.Method:Fibroblast cells of chicken embryonic were immersed separately in odontocem and MTA-Angelus solution for 24 and 72 hours.Cells viability was analyzed with MTT Assay.Result:There was a significant difference (p>0.05) in Odontocem and MTA-Angelus group compared to control.At the24-hour immersion, there was nosignificant difference between odontocem and MTA-Angelus.Conclusion:Odontocem and MTA-Angelus decreased the viability of fibroblast at 24 hours and increased them at 72 hours.;Background:MTA is proved tobe biocompatible and can be used for pulp capping treatment.Currently, calcium silicate based cement similar to MTA with steroid,called Odontocem has been developed.Objective:To compare effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability.Method:Fibroblast cells of chicken embryonic were immersed separately in odontocem and MTA-Angelus solution for 24 and 72 hours.Cells viability was analyzed with MTT Assay.Result:There was a significant difference (p>0.05) in Odontocem and MTA-Angelus group compared to control.At the24-hour immersion, there was nosignificant difference between odontocem and MTA-Angelus.Conclusion:Odontocem and MTA-Angelus decreased the viability of fibroblast at 24 hours and increased them at 72 hours.;Background:MTA is proved tobe biocompatible and can be used for pulp capping treatment.Currently, calcium silicate based cement similar to MTA with steroid,called Odontocem has been developed.Objective:To compare effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability.Method:Fibroblast cells of chicken embryonic were immersed separately in odontocem and MTA-Angelus solution for 24 and 72 hours.Cells viability was analyzed with MTT Assay.Result:There was a significant difference (p>0.05) in Odontocem and MTA-Angelus group compared to control.At the24-hour immersion, there was nosignificant difference between odontocem and MTA-Angelus.Conclusion:Odontocem and MTA-Angelus decreased the viability of fibroblast at 24 hours and increased them at 72 hours."

"ABSTRAK Latar Belakang: Berbagai material kaping pulpa berbahan dasar kalsium silikatterus dikembangkan, diantaranya semen berbasis kalsium silikat denganpenambahan steroid. Tujuan: Menganalisis efek penambahan steroid pada semenberbasis kalsium silikat terhadap viabilitas sel fibroblas. Metode: Sel fibroblasembrio ayam direndam dalam ekstrak larutan semen berbasis kalsium silikatdengan penambahan steroid dan MTA. Viabilitas sel dihitung dengan menggunakan uji MTT. Hasil: Terdapat perbedaan bermakna (p ≤ 0,05)viabilitas sel pada kelompok semen berbasis kalsium silikat dengan penambahansteroid dibandingkan kelompok kontrol dan MTA. Kesimpulan: Penambahansteroid menurunkan viabilitas sel. Terdapat peningkatan pada 72 jam, yang menandakan terjadinya proliferasi sel.ABSTRACT Background: Calcium silicate based materials are being developed continuously,one of them is calcium silicate based cement containing steroid. Objective: Toanalyze the effect of steroid addition in calcium silicate based cement onfibroblast cells viability. Methods: Chicken embryonic fibroblast cells wereimmersed in extract solution of calcium silicate based cement containing steroidand MTA. Viability was analyzed by MTT Assay. Results: Significant difference (p ≤ 0,05) of viability on calcium silicate based cement containing steroid groupwas found, compared to control and MTA group. Conclusion: Steroid additiondecrease viability. There was an increase in 72 hours, marking cells proliferation.;Background: Calcium silicate based materials are being developed continuously,one of them is calcium silicate based cement containing steroid. Objective: Toanalyze the effect of steroid addition in calcium silicate based cement onfibroblast cells viability. Methods: Chicken embryonic fibroblast cells wereimmersed in extract solution of calcium silicate based cement containing steroidand MTA. Viability was analyzed by MTT Assay. Results: Significant difference (p ≤ 0,05) of viability on calcium silicate based cement containing steroid groupwas found, compared to control and MTA group. Conclusion: Steroid additiondecrease viability. There was an increase in 72 hours, marking cells proliferation.;Background: Calcium silicate based materials are being developed continuously,one of them is calcium silicate based cement containing steroid. Objective: Toanalyze the effect of steroid addition in calcium silicate based cement onfibroblast cells viability. Methods: Chicken embryonic fibroblast cells wereimmersed in extract solution of calcium silicate based cement containing steroidand MTA. Viability was analyzed by MTT Assay. Results: Significant difference (p ≤ 0,05) of viability on calcium silicate based cement containing steroid groupwas found, compared to control and MTA group. Conclusion: Steroid additiondecrease viability. There was an increase in 72 hours, marking cells proliferation."

"Latar Belakang: Perawatan untuk perubahan warna gigi yang banyak dipilih masyarakat saat ini ialah tooth bleaching atau pemutihan gigi. Teknik home bleaching kerap menjadi pilihan masyarakat karena lebih murah serta tidak menimbulkan efek hipersensitivitas yang tinggi. Penggunaan bahan alami seperti buah-buahan dapat dimanfaatkan pada bidang kesehatan dan kecantikan termasuk untuk tooth bleaching. Buah tomat dapat dimanfaatkan sebagai agen pemutih tambahan untuk produk home bleaching karena mengandung agen pengoksidasi yang dapat mempercepat proses pemutihan gigi. Tujuan: Membuat material home bleaching hidrogen peroksida 3% dengan penambahan jus buah tomat dan mengetahui perbedaan warna gigi setelah aplikasi bahan bleaching. Metode: Dua puluh empat gigi premolar pasca ekstrasi diberi paparan bahan home bleaching. Sampel dibagi menjadi 4 kelompok dengan masing-masing terdiri dari 6 sampel. Kelompok A dipaparkan bahan bleaching hidrogen peroksida 3%, kelompok B hidrogen peroksida 3% dengan tambahan jus tomat 30%, kelompok C hidrogen peroksida 3% dengan tambahan jus tomat 75%, dan kelompok D dipaparkan bahan home bleaching komersial opalescence whitening gel PF 10%. Setiap kelompok dipaparkan 8 jam/hari selama 7 hari. Perubahan warna diukur sebelum dan sesudah paparan menggunakan kolorimeter dengan metode CIEL*a*b. Analisis data dengan uji statistik One-Way ANOVA dan Post Hoc Bonferroni. Hasil: Penelitian menunjukkan bahwa keempat kelompok dapat memutihkan gigi. Hasil perubahan warna ∆E*ab kelompok dengan hidrogen peroksida 3% 5,89, hasil ∆E*ab kelompok hidrogen peroksida 3% dengan tambahan jus tomat 30% 27,93, hasil ∆E*ab kelompok hidrogen peroksida 3% dengan tambahan jus tomat 75% 23,27, hasil ∆E*ab kelompok opalescence whitening gel PF 10% 10,67. Hasil ∆E*ab penambahan jus tomat lebih tinggi dibandingkan bahan hidrogen peroksida 3% dan bahan home bleaching komersial opalescence whitening gel PF 10%. Hasil ∆E*ab setiap kelompok terdapat perbedaan bermakna (p<0,05). Kesimpulan: Terdapat pengaruh penambahan jus buah tomat pada bahan bleaching hidrogen peroksida 3% terhadap perubahan warna gigi yang lebih cerah.

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Background: Tooth bleaching is one of the treatment that many people choose in the management for tooth discoloration. Home bleaching technique often chosen because cheaper and do not cause high hypersensitivity effects. The use of natural ingredients such as fruits can be utilized in the health and beauty sector, including for tooth bleaching. Fruits, such as tomato can be used as an additional whitening agent for home bleaching products because it contains an oxidizing agent which can speed up the teeth whitening process. Objective: To make 3% hydrogen peroxide bleaching at home with the addition of tomato juice and find the difference in tooth colour after application of the bleaching agent. Methods: Twenty four post-extraction premolars were exposed to home bleaching agents. The sample was divided into 4 groups with 6 samples each. Group A was exposed to 3% hydrogen peroxide bleaching agent, group B was exposed to 3% hydrogen peroxide with the addition of 30% tomato juice, group C was exposed to 3% hydrogen peroxide with the addition of 75% tomato juice, and group D was exposed to commercial home bleaching agent opalescence whitening gel PF 10% . Each group was exposed 8 hours/day for 7 days. Colour changes were measured before and after exposure using a colorimeter with the CIEL*a*b method. Data analysis with One-Way ANOVA and Post Hoc Bonferroni statistical tests. Results: Research shows that all four groups can whiten teeth. Color change results in hydrogen peroksida 3% ∆E*ab 5,89, ∆E*ab % hydrogen peroxide with the addition of 30% tomato juice 27,93, ∆E*ab % hydrogen peroxide with the addition of 75% tomato juice 23,27, and ∆E*ab opalescence whitening gel PF 10% 10,67. The results of the discoloration of the ∆E*ab group with 3% hydrogen peroxide and the addition of tomato juice were higher than those of 3% hydrogen peroxide without addition of tomato juice and exposed to commercial home bleaching agents, opalescence whitening gel PF 10%. Color change results between groups significantly different (p<0,05). Conclusion: There is an effect of adding tomato juice to 3% hydrogen peroxide bleaching agent on teeth discoloration that is brighter."

"ABSTRAKPenelitian ini ditujukan untuk mengetahui dan membandingkan pengaruh pastagigi yang mengandung theobromine dan hydroxyapatite terhadap kekerasan mikroemail gigi setelah direndam dalam minuman berkarbonasi. Penelitian inimenggunakan 21 spesimen mahkota gigi premolar satu rahang atas. Spesimendirendam dalam minuman berkarbonasi kemudian disikat dengan pasta gigi yangmengandung theobromine dan hydroxyapatite. Uji Kruskal-Wallis dan Mann-Whitney menunjukkan perubahan kekerasan yang signifikan setelah penyikatanselama 9 menit 20 detik menggunakan kedua pasta gigi pasca direndam dalamminuman berkarbonasi selama 10 menit. Peningkatan nilai kekerasan mikro emailgigi pada kelompok hydroxyapatite lebih besar dibandingkan kelompoktheobromine (p<0,05).

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ABSTRACTThis study is aimed to analyze the effect of theobromine and hydroxyapatitetoothpaste to enamel microhardess after immersion in carbonated drink. Thisstudy uses 21 upper first premolar crown specimen which is immersed incarbonated drink then brushed with theobromine and hydroxyapatite containingtoothpaste. Kruskal-Wallis and Mann-Whitney test shows significant difference ofmicrohardness after brushing for 9 minutes and 20 seconds using both toothpastesafter immersion in carbonated drink for 10 minutes with higher number inhydroxyapatite group than theobromine group (p<0,05)."

"ABSTRAK Latar Belakang : Enterococcus faecalis merupakan bakteri yang mampu membentuk biofilm dan banyak ditemukan pada kasus kelainan periapeks.Tujuan : Melihat daya antibakteri campuran triantibiotik dibandingkan denganklorheksidin 2% terhadap E. faecalis dalam biofilm. Metode : Menilai kekeruhanlarutan E. faecalis dalam biofilm pasca pemaparan bahan uji, dengan ELISAreader. Hasil : Terdapat daya antibakteri campuran triantibiotik terhadap biofilmbakteri E. faecalis tetapi tidak terdapat perbedaan bermakna dengan klorheksidin2% (p>0.05). Kesimpulan : Daya antibakteri campuran triantibiotik terhadapbiofilm E. faecalis sebanding dengan klorheksidin 2%. ABSTRACT Background : Enterococcus faecalis has the ability to form biofilm and is oftenfound in cases of periapical lesions. Aim: To analyze the effectivity of triantibioticmixture compared to 2% chlorhexidine against biofilm of E. faecalis. Method :Score the turbidity of E. faecalis in biofilm after immersion in antibacterialagent, with ELISA reader. Result : Triantibiotic mixture has antibacterialeffectivity against E. faecalis biofilm but has no significant difference compared to2% chlorhexidine (p>0.05). Conclusion : Antibacterial effectivity of triantibioticmixture against E. faecalis biofilm is equal to2% chlorhexidine. ;Background : Enterococcus faecalis has the ability to form biofilm and is oftenfound in cases of periapical lesions. Aim: To analyze the effectivity of triantibioticmixture compared to 2% chlorhexidine against biofilm of E. faecalis. Method :Score the turbidity of E. faecalis in biofilm after immersion in antibacterialagent, with ELISA reader. Result : Triantibiotic mixture has antibacterialeffectivity against E. faecalis biofilm but has no significant difference compared to2% chlorhexidine (p>0.05). Conclusion : Antibacterial effectivity of triantibioticmixture against E. faecalis biofilm is equal to2% chlorhexidine. ;Background : Enterococcus faecalis has the ability to form biofilm and is oftenfound in cases of periapical lesions. Aim: To analyze the effectivity of triantibioticmixture compared to 2% chlorhexidine against biofilm of E. faecalis. Method :Score the turbidity of E. faecalis in biofilm after immersion in antibacterialagent, with ELISA reader. Result : Triantibiotic mixture has antibacterialeffectivity against E. faecalis biofilm but has no significant difference compared to2% chlorhexidine (p>0.05). Conclusion : Antibacterial effectivity of triantibioticmixture against E. faecalis biofilm is equal to2% chlorhexidine. ;Background : Enterococcus faecalis has the ability to form biofilm and is oftenfound in cases of periapical lesions. Aim: To analyze the effectivity of triantibioticmixture compared to 2% chlorhexidine against biofilm of E. faecalis. Method :Score the turbidity of E. faecalis in biofilm after immersion in antibacterialagent, with ELISA reader. Result : Triantibiotic mixture has antibacterialeffectivity against E. faecalis biofilm but has no significant difference compared to2% chlorhexidine (p>0.05). Conclusion : Antibacterial effectivity of triantibioticmixture against E. faecalis biofilm is equal to2% chlorhexidine. "

"Latar Belakang: Ekstrak etanol temulawak (Curcuma xanthorrhiza Roxb.) telah terbukti secara in vitro memiliki khasiat sebagai anti Candida albicans (C.albicans). Dalam upaya pengembangan tanaman obat tersebut sebagai obat herbal terstandar anti C.albicans, ekstrak etanol temulawak telah diformulasikan menjadi obat tetes oromukosa. Temulawak mengandung kurkumin yang merupakan senyawa polifenolik berwarna kuning yang dapat menyebabkan diskolorasi gigi. Tujuan: Mengetahui pengaruh paparan obat tetes ekstrak etanol temulawak terhadap warna email gigi. Metode: Gigi premolar tanpa karies dan defek struktural dicelupkan dalam obat tetes ekstrak etanol temulawak, CHX 0,2%, dan akuades selama 1 menit kemudian dibilas dan direndam dalam akuades selama 10 menit pada suhu 37oC. Tahapan dilakukan sebanyak 42 siklus (simulasi penggunaan 2 minggu) dan 63 siklus (simulasi penggunaan 3 minggu). Analisis warna dilakukan menggunakan colorimeter pada 3 tahap waktu yaitu sebelum paparan, setelah paparan, dan setelah penyikatan gigi. Nilai yang didapatkan berupa ΔE yang menunjukkan selisih nilai pengukuran warna email sebelum dan setelah paparan obat serta sebelum dan setelah penyikatan. Hasil: Pada tahap waktu T1-T3 simulasi penggunaan 2 minggu dan 3 minggu, nilai ΔE>3.3 pada ketiga kelompok sehingga terlihat adanya perubahan warna yang signifikan antara warna gigi awal dan setelah penyikatan gigi. Terdapat perubahan warna gigi yang signifikan setelah dilakukan penyikatan dengan pasta gigi. Kesimpulan: Obat tetes ekstrak etanol temulawak mengakibatkan perubahan warna email gigi yang signifikan. Penyikatan gigi dapat mengurangi efek perubahan warna pada email gigi.

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Background: Javanese Turmeric (Curcuma xanthorrhiza Roxb.) ethanol extract is known to have antifungal properties against Candida albicans (C.albicans) based on in vitro studies. The next step in developing a standardised herbal medicine is by formulating Javanese Turmeric Ethanol Extract into oromucosal drops. Curcumin found in javanese turmeric is a yellowish polyphenolic compound that has the potential to cause staining on the enamel.

Objective: This study is aimed to evaluate the effect Javanese Turmeric ethanol extraxt oromucosal drops on discoloration of the dental enamel.

Method: Premolars with no caries and structural defects are immersed in the Javanese Turmeric ethanol extract oromucosal drops, a 0,2% CHX mouthwash, and distilled water for 1 minute. After rinsing, they are then immersed in distilled water for 10 minutes at 37oC. The method mentioned is repeated for 42 cycles (2-week simulation) and 63 cycles (3-week simulation). Color assessment is done using a colorimeter at three different time points: before immersion, after immersion, and after brushing. Results will be shown as ΔE which is the color difference of enamel before and after immersion, as well as before and after toothbrushing.

Result: At time point T1-T3 for the 2-week and 3-week simulation, the ΔE score is greater than 3.3 on all three groups indicating a significant color difference before immersion and after toothbrushing. A significant color difference is observed after toothbrushing with toothpaste.

Conclusion: Javanese Turmeric ethanol extract oromucosal drops cause a significant tooth discoloration. Brushing had significant effect on removal of induced stains."