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"Dengan semakin banyak beredarnya campuran asam asetil salisilat dan asam askorbat dalam sediaan tablet efervesen, maka perlu dikembangkan suatu metode yang sensitif dan mudah didalam menganalisis kadar sediaan tersebut. Metode yang digunakan adalah Kromatografi CairKinerja Tinggi (KCKT) dengan menggunakan kolom C-18 ODS (3,9 mm x 300 mm); detektor UV-Vis (panjang gelombang 280 nm); fase gerak asetonitrildapar fosfat pH 3,5 (30:70, v/v) dengan laju alir 1,2 mL/menit.Parameterparameter analitik menunjukkan bahwa metode ini dapat digunakan untuk menetapkan kadar asam asetil salisilat dan asam askorbat secara bersamaan. Pada penetapan kadar sampel diperoleh hasil kadar asam asetil salisilat 94,59 ± 1,33% dan kadar asam askorbat 104,58 ± 1,01%."

"As the food and beverages industry grows in Indonesia, there also has been an increase in the soft-drinks production in the society. There are elements often added into the drinks; such as caffeine, artifical sweetener and preservatives,which the content should be monitored. Because, if they are over-used, they will be hazardous to health. The purpose of this research is to obtain the optimum analysis condition for determining the content of saccharin, aspartame, benzoic acid, sorbic acid and caffeine, which are in the soft-drinks, using the reversed phase High-Performance-Liquid-Chromatography (HPLC). In this study, the condition used are Latek 18 column (15 cm x 4.0 mm), mobile phase as a mixture of acetonitrile and acetat buffer pH 5(5:95), flow rate 1,0 ml/minutes and detected by a 254 nm length-wave. The detection limit discovered by this method are for saccharin, benzoic acid, sorbic acid, caffeine and aspartame, respectively, are 0,2 ppm; 0,2 ppm; 0,007 ppm; 0,142 ppm; and 6,5 ppm. Whereas, the quantitative limit for saccharin, benzoic acid, sorbic acid, caffeine and aspartame, respectively, are 0,689 ppm; 0,852 ppm; 0,027 ppm; 0,452 ppm; 25,2 ppm. The calibration curve ranged between 1-60 ppm for saccharin and benzoid acid, 1-40 ppm for caffeine, 0.05-2 ppm for sorbic acid, and 30-100 ppm for aspartame. The investigation has been done for five (5) brands od soft-drinks. The analysis results are sample A contains caffeine 96,66 ppm, sample B contains saccharin 112,13 ppm, benzoic acid 206,81 ppm, and caffeine 130,63 ppm. Sample C contains benzoic acid 10,83 ppm and caffeine 97,66 ppm. Sample D contains benzoic acid 163,78 ppm, caffeine 101,52 ppm, and aspartame 231,20 ppm. The amounts of saccharin, benzoic acid, caffeine, and aspartame which has been found in the sample, do not exceed the tolerance limit of usage, whereas the amount of benzoic acid which has been found in sample B exceed the tolerance limit of usage."

"Sediaan sirup multivitamin banyak beredar di pasaran dewasa mi; dan digunakan untuk inengobati penyakit yang disebabkan oleh kekurangan vitamin,, mencegah defisiensi vitamin dan untuk merangsang perturnbuhan.Komponen-komponen yang umumnya terdapat dalam sirup multivitamin adalab : vitamin Bi, vitamin B2, vitamin B6, nikotinarnid, vitamin A, vitamin B12, vitamin C, Vitamin D, Ca-panthotenat dan asarn amino. Sulit untuk nienetapkan ka dar beberapa koinponen tersebut serempaR tanpa melakukan pemisahan terlebih dahulu. Metoda krointografi cair kinerja tinggi digunakan untuk rnemisahkan komponen-kornponen yang terdapat dalam sirup multivitarnin.Tujuan penelitian mi adalah untuk memperoleh kondisi analisa yang optimum untuk penetapan kadar vitamin Bi, vitamin 32, vitamin 36 dan nikbtinamid secara serepac, yang terdapat dalam sirup multivitamin.6 sampel sirup multivitamin (A,B,C) Droduk PMDN dan (D',.E,.F) produk PMA, telah ditetapkan kadar vitamin Bi, vi tamin B2, vitamin B6 dan nikotinamid-nya; dengan kondisi analisa : fasa gerak campuran metanol-air yang mengandung 5 mN Na-oktansulfonat, 1,36% YB2PO4: pH 3,5 ( 3:7 ); kecepatan aliran. 0,5 ml per menit; dan deteksi UV (270 nm.).Dari 6 sampel tersebut, kadar vitamin B2 cderung rendah ( 20,41-47,44% ) cian pada tiga sampel (L, .E F) ka dar nikotinamid juga cenderung rendab ( 86,29-88,30% ).

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There are a lot of multivitamin syrup preparations On the market to day, they are used in therapy of avitaminosis diseases, preventing vitamin deficiency and stimulatinggrowth.

The common componentst of multivitamin syrup are vitamin Bi, vitamin B2, vitamin B6, niacinamide, vitamin A, vitamin B12, vitamin C, vitamin D, Ca-panthotenate and amino acid. The simultaneous determination of--the components without preceeding separation are complicated. The method of high performance liquid chromatography is used to separa te the components of multivitamin syrup.

The purpose of this study is to find an optimal condition, for simultaneous determination of vitamin Bi, vitamin B2, vitamin B6 and niacinamide in multivitamin syrup.

6 sample of multivitamin syrups .( A,B,C from PJ4DN 1 s ma nufacture and D,L,F from PMA's manufacture); vitamin Bl, vi tamin B2, vitamin B6 and nia,cinamide concentration have been- determinated. The conditions mobile phase are 30% inetha nol-70% 5:mN sodium octanesulfonate in 1,36% KH 2PO4 pE 3,5; flow rate 0,5 ml per minute and detection by UV at 270 rim.

Concentration of vitamin Bi in all sample are low (20,41-47,44%) and concentration of niacinamide in three sample (L',E & F) are low as well (86,29-88,90%)."

"ABSTRAKGlukosamin hidroklorida dan kondroitin sulfat merupakan senyawa glikosaminoglikan (GAGs) yang merupakan komponen struktural utama dari tulang yang akan membentuk proteoglikan. Kedua senyawa ini dapat merawat kesehatan tulang dengan menstimulasi sintesis cairan sinovial dan menghambat degradasi kartilage persendian, sehingga dapat digunakan untuk terapi osteoartritis. Penelitian ini bertujuan untuk memperoleh metode analisis yang selektif untuk penetapan kadar glukosamin hidroklorida dan kondroitin sulfat dalam sediaan tablet dan krim. Setelah diderivatisasi menggunakan pereaksi ortoftalaldehida dan 2-merkaptoetanol (OPA/2-ME), sampel dianalisis menggunakan kromatografi cair kinerja tinggi (KCKT) dengan detektor fluoresensi pada panjang gelombang eksitasi 335 nm dan panjang gelombang emisi 445 nm. Glukosamin mempunyai gugus amin primer sehingga dapat diderivatisasi dengan OPA/2-ME, sedangkan kondroitin mempunyai gugus asetil pada gugus amin, sehingga perlu dilakukan deasetilasi menggunakan natrium hidroksida untuk memutus gugus asetil. Fase gerak yang digunakan tetrahidrofuran 0,25% dalam air-asetonitril (87:13) dengan laju alir 1,5 mL/menit. Kondisi analisis yang telah dioptimasi kemudian divalidasi mencakup akurasi, presisi, linieritas, selektivitas, batas deteksi, dan batas kuantitasi. Hasil menunjukkan kadar rata-rata glukosamin hidroklorida dan kondroitin sulfat pada sediaan tablet dan krim adalah 92,76%; 96,11% dan 101,15%; 100,33% memenuhi syarat keberterimaan.

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ABSTRAKGlucosamine hydrochloride and chondroitin sulphate are glycosaminoglycans (GAGs) compound which is a major structural component of bone that form proteoglycans. Both of these compounds can take care of bone health by stimulating the synthesis of synovial fluid and inhibit the degradation of joint cartilage, so it can be used for the treatment of osteoarthritis. The aimed of this study were obtain selective analytical method for the determination of glucosamine hydrochloride and chondroitin sulphate levels in tablet and cream dosage forms. After derivatization using orthophtalaldehyde and 2-mercaptoethanol (OPA/2-ME), samples were analyzed using high performance liquid chromatography (HPLC) with fluorescence detector at excitation wavelength of 335 nm and emission wavelength of 445 nm.. Glucosamine has a primary amine group that can be derivatized with OPA/2-ME, while chondroitin having an acetyl group at the amine group, so we needed deacetylation using natrium hydroxide to break the acetyl group. The mobile phase used tetrahydrofuran 0.25% in water-acetonitrile (87:13) with a flow rate 1.5 mL/min. Analysis conditions have been optimized, validated in terms of accuracy, precision, linearity, selectivity, limit of detection, and limit of quantitation. The results showed average levels of glucosamine hydrochloride and chondroitin sulphate in tablet and cream dosage forms were 92.76%; 96.11% and 101.15%; 100.33% and fulfilled the acceptance criteria."