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"[Ebi merupakan udang kering berukuran kecil yang dapat dikonsumsi secarakeseluruhan. Ebi diduga memiliki kadar kalsium tinggi meski belum ada penelitianspesifik pada genus tertentu. Penelitian ini bertujuan mengetahui kadar kalsium padaMetapenaeus sp. secara akurat serta menganalisis pengaruh metode preparasi (ovendan non oven) dan metode isolasi (pengocokan, digesti asam, dan pengabuan)terhadap pengukuran dengan ISE dan AAS. Hasil pengukuran didapatkan kadarkalsium tertinggi 6.769 ppm (oven) dan 7.785 ppm (non oven) dengan isolasi terbaikadalah digesti asam. Terdapat perbedaan serta korelasi kadar kalsium antar metodepreparasi dan isolasi, sehingga disimpulkan kedua metode berpengaruh terhadappengukuran kadar kalsium ebi.;Ebi is dried small sized shrimp consumed as a whole. Ebi is assumed to have highcalcium level even there has been no research on specific genus yet. This researchaimed to know exact calcium level in Metapenaeus sp., analyze the influence ofpreparation (oven and non oven) and isolation (dilution, acid digestion, and dryashing) methods toward ISE and AAS measurement. Results showed highest calciumlevel 6,769ppm (oven) and 7,785ppm (non oven) with acid digestion as best isolationmethod. There were differences and correlation among preparation and isolationmethods; and concluded both methods influenced calcium level measurement in ebi., Ebi is dried small sized shrimp consumed as a whole. Ebi is assumed to have highcalcium level even there has been no research on specific genus yet. This researchaimed to know exact calcium level in Metapenaeus sp., analyze the influence ofpreparation (oven and non oven) and isolation (dilution, acid digestion, and dryashing) methods toward ISE and AAS measurement. Results showed highest calciumlevel 6,769ppm (oven) and 7,785ppm (non oven) with acid digestion as best isolationmethod. There were differences and correlation among preparation and isolationmethods; and concluded both methods influenced calcium level measurement in ebi.]"

"Ha Lam usaha mencari sumber sterol yang potensialsebagai bahan dasar untuk memproduksi hormon steroid,telah diteliti kepaia udang putih (Penaeus merguiensis).Panelitian dilakukan dengan tujuan menilaikemungkinan penerapan metoda enziinatik CHOD-PAP terhadapudang dengan pereaksi siap pakai C reagen Kit ) yangdigunakan untuk penetapan kadar Kolesterol dalam darah.sebagai pembanding digunakan metode Kromatograf i Gasdengan fase diam SE-30 5 % pada Chromosorb W-HP dantemperatur kolom 260°C. Juga untuk menentukan bentukKolesterol dalam udang, dalam bentuk yang larut dalaiaair atau tidaJ^ serta untuk mengetahui apakah pereaksienzimatik CHOD-PAP dapat bereaksi dengan Fitosterol.Dengan metode enzimatik CHOD-PAP dan KromatografiGas diperoleh kadar Kolesterol dalam ekstrak eter2,08 ± 0,55 dan 2,07 ± 0,41 mg/g sampel. Dengan metodaenzimatik CHOD-PAP dan Kromatografi Gas diperoleh kadarKolesterol dalam ekstrak air 1,98 ± 0,34 dan 1,82 ±0,31 mg/g sampel. nPada ekstraksi Kolesterol dengan eter didugaada zat yang mengganggu penetapan kadarnya secara enzimatik CHOD-PAP sehingga disimpulkan bahwa metoda ini belumdapat digunakan untuk ekstrak eter.Kolesterol dalam udang berada dalam bentuk yanglarut dalam air ± 90 %. Fitosterol ternyata dapat bereaksi dengan pereaksi enzimatik CHOD-PAP sehingga reagenini tidak spesifik untuk penetapan kadar Kolesterol.

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To seek potential sources of sterols as raw

material to produce steroid hormones, head of white

shrimp ( Penaeus merguiensis ) has been investigated.

The investigation is done for evaluating the

possibility of application of CHOD-PAP enzyifiatic method

on shrimp using ready for use x'eagent available for

the determination of Cholesterol in sera. As a standard

Gas Chromatogreiphic method is used with SE-30 5 % on

Chromosorb W-HP, column temperature 250°C. To know

whether CHOD-PAP enzymatic reagent could react with

Phytosterols.

By enzimatic CHOD-PAP and Gas Chromatographic

methods on the ether extract Cholesterol contents of

2,08 ± 0,55 and 2,07 ± 0,41 mg/g sample were obtained.

By enzymatic CHOD-PAP and Gas Chromatograpic methods

on the water extract Cholesterol contents of 1,98 ±

0,34 and 1,82 ± 0,31 mg/g sample were obtained.

When extracted with ether, a substance

interfering with CHOD-PAP enzymatic determination

seemed to be present so that it' s concluded that this

method couldn't be used yet for the ether extract.

Cholesterol in shrimp exists in soluble form for about

90 %. Infact, Fhytosterol could react with enzymatic

CHOD-F•? reagent so that this reagent isn ^ t spesific

for detexmsination of Cholesterol."

"Isolation of total sterol from waste product of soy bean cake has been conducted, followed by biotransformation to 1,4-androstadien-3,17-dione (ADD). The waste product consist of; β-sitosterol, stigmasterol, kaemfesterol, which are isolated by column chromatography technique using silica gel as stationary phase and chloroform as mobile phase. Biotransformation was conducted by using Mycobacterium phlei DSM 43286 in the present of α, α?- dipiridil as an inhibitor with concentration of 0,5; 1; 1,5; 2,0 mM. The main product of biotransformation were ADD and pregnenolon. The optimum yield of ADD 0,48% is achieved by adding 1,5 mM α, α?- dipiridil are two hours after addition of substrate and 72 hours of incubation time."