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"Telah dilakukan uji sitotoksik metabolit sekunder kapang endofit 1.2.11 tanaman Brucea javanica (L. ) Merr. Sampel tanaman diambil dari Cianjur, bagian tanaman yang digunakan adalah buah. Uji sitotoksik dilakukan terhadap sel Ruji, NS-l, sel HeLa dan sel Vero. Pengamatan dilakukan selama 24 jam dan 48 jam dengan menghitung sel ludup menggunakan metode tripan biru. Penghitungan l CM dilakukan secara aritmatikal dengan rumus Ricd and Muench. Untuk melihat mekanisme kerja pada proses sitotoksik dilakukan teknik pengecatan DNA menggunakan etidium bromida dan acridine orange. Dari penelitian ini diperoleh lCft) terhadap sel Raji 58,35 fjg/ml, 88,39yg/ml; IC50sel NS-1 162,09 pg/ml, 66,24 p. g/ml; IC^ sel HeLa 361,21 pg/ml, 219,97 pg/ml. IC^sel Vero 1075.18 ug/ml, 656,82 ng/ml. Pengamatan dilakukan dalam waktu 24 jam dan 48 jam. Mekanisme kerja dari metabolit sekunder kapang endofit 1.2. 11 terhadap sel NS-1 cenderung melalui mekanisme apoptosis. (Med J Indones 2006; 15:137-44)

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Cytotoxic assay of secondary metabolite endophytic fungus 1.2.11 from Brucea javanica (L.) Merr has been carried out. Brucea Javanica fruit collected from Cianjur was used in this experiment. Cytotoxic assay was done on Raji, NS-1, HeLa and Vero cells. The observation was done for 24 hours and also for 48 hours. I CM was calculated using the Rich and Muench theory. To observe the working mechanism ofcytotoxic process, DNA staining with etidium bromide and acridine orange was conducted. The cytotoxic assay of endophytic fungi 1.2.11 showed an ICft) of 58.35p.g/ml, 88.39 pg/ml on Raji ceil; 162.09 pg/ml, 66.24 pg/ml on NS cell; 361.21 fjg/ml, 219.97 fjg/ml on HelM cell; and lastly 1075.18 fjg/ml, 656.82 /jg/ml on Vero cell after 24 and 48 hour incubation respectively. The results of this study showed that secondary metabolite of endophytic fungus 1.2.11 has selective cytotoxic effect towards cancer cell and also showed that it might cause apoptosis in NS-1 cell. (Med J Indones 2006; 15:137-44)"

";Gastrodia crispa sebagai anggrek holomycotrophic endemik pegunungan Jawa Barat memiliki habitat pada tanah dengan serasah bambu dan pertumbuhannya sangat dipengaruhi oleh mikoriza. Kebutuhan kondisi lingkungan yang spesifik dan keberadaannya yang endemik diduga memiliki simbiosis dengan fungi tertentu. Isolasi, identifikasi, dan uji pertumbuhan perlu dilakukan untuk mengetahui  mikoriza simbion G. crispa dan pertumbuhan optimalnya. Penelitian telah berhasil mengisolasi dan mengidentifikasi mikoriza simbion G. crispa yang diperoleh dari Taman Nasional Gunung Halimun-Salak dan Taman Nasional Gunung Gede-Pangrango. Isolasi dilakukan berdasarkan teknik tanam langsung dan diinkubasi pada suhu 25 ^oC. Empat isolat berhasil diisolasi dari tiga sampel umbi semu G. crispa. Identifikasi fenotipik yang dibandingkan dengan kunci identifikasi menduga isolat merupakan genus Acremonium, Penicillium, Trichoderma, dan mycelia sterilia. Uji pertumbuhan menggunakan variasi medium dan suhu inkubasi dilakukan pada medium PDA dan PDB modifikasi di suhu 9 ^oC, 25 ^oC, dan 30 ^oC selama 25 hari. Medium PDA dan PDB modifikasi 1 menggunakan pelarut filtrat tanah, sementara PDA dan PDB modifikasi 2 menggunakan pelarut filtrat tanah dengan serasah bambu. Hasil uji keempat isolat mikoriza mendapatkan pertumbuhan optimal pada PDA dan PDB modifikasi di suhu 25 ^oC. Pertumbuhan Acremonium sp., Penicillium sp., dan Trichoderma sp. di medium PDA dan PDB modifikasi 1 dengan PDA dan PDB modifikasi 2 tidak berbeda signifikan, sementara pertumbuhan mycelia sterilia lebih optimal pada medium PDA dan PDB modifikasi 2.

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Gastrodia crispa is an endemic holomycotrophic orchid of West Java mountain area found on soil with bamboo leaf litter and its growth is greatly affected by mycorrhiza. The specific requirement of environmental condition and its endemic presence, might have a symbiosis with specific fungi. Isolation, identification, and growth assay were carried out to identify the mycorrhizae and their optimal growth condition. The research has succeeded in isolation and identification of the symbiont mycorrhizae G. crispa from Gunung Halimun-Salak National Park and Gunung Gede-Pangrango National Park. Isolation was carried out using readily cultured isolation technique and incubated at 25 oC. Four mycorrhizae were isolated from three G. crispa pseudotuber samples. Phenotypic identification compared to the key identification assumed that the isolate belongs to the genus Acremonium, Penicillium, Trichoderma, and mycelia sterilia. Variation of medium and incubation temperature were assayed using the modified PDA and PDB medium with three variations of incubation temperature 9 oC, 25 oC, and 30 oC for 25 days. Soil filtrate was used to dissolve modified PDA and PDB 1, while bamboo leaf litter with soil filtrate was used to dissolve modified PDA and PDB 2. The results show that four isolates mycorrhizae had optimal growth on modified PDA and PDB at the temperature of 25 oC. The growth results of Acremonium sp., Penicillium sp., Trichoderma sp. on modified PDA and PDB 1 with modified PDA and PDB 2 did not show a significant distinction, while mycelia sterilia had optimal growth on modified PDA and PDB 2."

"This study aims to examine the factors influence of IPO performance to IPO returns. This study tries to define an appropriate mechanism to determine the factors that can influence IPO return in Indonesia, as represented by the companies in Indonesia Stock exchange (IDX). Factors influencing IPO performance are representative by six variables, namely firm size, age of the firm, total share ofter to public type of investor, debt level of the firm and price ratio of offeringp price and earning per share. IPO returns factors are represented by one variable, which is short term return (initial return) . Data for this study were taken from compaines listed in IDX year 1996 -2006. Based on the research finding and analysis, it is found that the factors scrutinzed by the researcher (firm size, age of the firm, Total share offter to public, type of investor, debt level and price ratio) cannot influence the IPO return in Indonesia market: This possibly occurs because the invesment condition cannot can be influenced by the internal condition of the company. Furthermore, the investor especially outsider one, probably still has doubts on external conditions such unstable, economic condition, political condition, government regulation and security conditions in Indonesia thus, it is possible that external condition can influence the IPO return in Indonesia."

"ABSTRAKPenelitian ini bertujuan untuk meneliti hubungan kepuasan berpacaran dengan kebahagiaan subyektif dimoderatori oleh keinginan untuk menikah pada individu dewasa muda yang berpacaran berbeda agama. Partisipan dalam penelitian ini sebanyak 145 orang. Penelitian ini menggunakan alat ukur yaitu Relationship Assessment Scale RAS , Scale of Positive and Negative Experience SPANE , dan Satisfaction with Life Scale SWLS . Analisis data dilakukan menggunakan moderasi model satu yang dikemukakan oleh Hayes. Hasil dalam penelitian ini menunjukkan bahwa terdapat interaksi antara keinginan untuk menikah dalam hubungan kepuasan berpacaran dan kebahagiaan subyektif pada individu dewasa muda yang menjalani hubungan berpacaran berbeda agama c = 0,047; t = 2,674; sig. = 0,008 .

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ABSTRACT

This study aims to examine the correlation between relationship satisfaction and subjective well being moderated by willingness to marry among young adults in deferent religion relationship. Partisipants in this study is 145 persons. Measurement in this study using Relationship Assessment Scale RAS , Scale of Positive and Negative Experience SPANE , and Satisfaction with Life Scale SWLS . Data analysis using moderated model one which proposed by Hayes. The result of this study indicates that there is an interaction between the willingness to marry in the relationship of relationship satisfaction and subjective well being in young adults in deferent religion relationship c 0,047 t 2,674 sig. 0,008 ."

"Perkembangan teknologi setiap tahunnya semakin berkembang lebih maju dan cepat, salah satunya adalah dalam hal penggunaan internet. Dengan semakin banyaknya pengguna internet dan pelaku industri telekomunikasi di Indonesia sehingga menimbulkan persaingan ketat antar perusahaan telekomunikasi. Oleh karena itu, perusahaan telekomunikasi harus meningkatkan daya saing mereka dengan cara mempertahankan pelanggan dan pemanfaatan teknologi, serta melakukan pengukuran tingkat kematangan dalam skala aktivitas atau proses bisnis. Penelitian ini bertujuan untuk mengetahui perilaku pelanggan melalui segmentasi pelanggan berdasarkan Customer Lifetime Value (CLV) dengan pendekatan model Recency, Frequency, dan Monetary (RFM), melakukan pengukuran tingkat kematangan proses bisnis terkait Customer Care berdasarkan framework eTOM, dan merancang rekomendasi peningkatan loyalitas pelanggan untuk setiap segmen. Metode K-Means Clustering digunakan untuk melakukan segmentasi pelanggan yang menghasilkan 4 klaster dan nilai CLV dipakai untuk menentukan nilai dari setiap klaster pelanggan dengan variabel RFM. Kemudian pemetaan klaster menggunakan Customer Value Matrix (CVM) dilakukan untuk memastikan karakteristik klaster pelanggan. Kemudian nilai CLV digunakan sebagai bobot pengukuran untuk melakukan pemeringkatan dengan metode TOPSIS (Technique for Order Preference by Similarity to Ideal Solution) pada 10 alternatif yang diusulkan kepada para ahli untuk dapat diterapkan oleh perusahaan telekomunikasi sehingga meningkatkan loyalitas pelanggan dan meningkat daya saing perusahaan.

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The development of technology is growing more advanced and faster every year, including in terms of internet usage. The number of internet users and telecommunication industries in Indonesia increasing continuously, it has created high competition between telecommunication companies. Therefore, telecommunication company need to improve their competitiveness by retain loyalty customers and utilizing technology and also have a maturity level measurement in activities or business processes. The purpose of this study are to determine customer behavior through customer segmentation based on Customer Lifetime Value (CLV) using the Recency, Frequency, and Monetary (RFM) model, measure the maturity level of business process related to Customer Care based on the eTOM framework, and design recommendations to increase customer loyalty for each segment. Customers are segmented using the K-Means Clustering method, which yields four clusters, and the CLV value is used to determine the value of each customer cluster with the RFM variable. The Customer Value Matrix (CVM) is used to determine the characteristics of the customer clusters through cluster mapping. Then the CLV value is used as a measurement weight to rank with TOPSIS (Technique for Order Preference by Similarity to Ideal Solution) method on the 10 alternatives proposed to the experts to be implemented by telecommunication company so they can increase customer loyalty and increase company competitiveness."

"Most sales professionals make the mistake of using the same sales patterns over and over. But since all customers are different, true pros know they must tailor their methods to the buyer if they want to make their numbers every year. ProActive Selling gives readers the tools they need to adapt their approach and maintain control at every stage of the sale. Thoroughly revised and updated, the second edition shows salespeople how to: • Qualify and disqualify prospects sooner to focus on the most promising accounts • Examine buyers’ motivations from every angle • Quantify the value proposition early • Double the number of calls returned from prospective customers • Appeal to the real decision-makers • Use technology (e.g. cloud, video, social media, and more) to generate leads and shorten sales cycles • Increase the effectiveness of every interaction Featuring dozens of enlightening examples and the author’s 17 exclusive, practical selling tools, ProActive Selling gives sales professionals the edge they need to exceed their goals—with any company, in any industry."

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