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"Many microorganisms capable of degrading petroleum components have been isolated and few of the seem to be important for petroleum biodegradation in natural environments...."

"The ability of native bacteria to utilize diesel fuel as the sole carbon and energy source was investigated in this research...."

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Latar Belakang : Infeksi intraabdomen (IIA) merupakan respons inflamasi peritoneum oleh mikroorganisme penyebab sepsis dan kematian kedua terbanyak di ruang intensive care unit (ICU).^1,2Complicated intra-abdominal infection worldwide observational study (CIAOW) menunjukkan angka kematian infeksi intraabdomen komplikata sebesar 10,5%. Terapi antimikroba atau antibiotik merupakan hal penting dalam penanganan IIA. Dalam mendukung keberhasilan penanganan kasus IIA dibutuhkan informasi akurat mengenai karakteristik dan pola kepekaan terhadap antibiotik yang dapat digunakan sebagai acuan penggunaan antibiotik pada kasus IIA. Tujuan: Mendapatkan karateristik mikrobiologis dan klinis pada kasus IIA dan mengetahui hubungan antara faktor klinis dan mikrobiologi dengan luaran klinis pasien.

Metode: Data diambil secara prospektif  dengan desain pontong lintang. Sampel penelitian berupa jaringan intraabdomen dan darah yang diambil saat pembedahan. Uji identifikasi dan uji kepekaan dilakukan untuk deteksi bakteri aerob dan bakteri anaerob. Hasil:. Infeksi intraabominal komplikata lebih banyak dibandingkan IIA non-komplikata (63,63%), kasus sepsis (63,63%) dan peritonitis (45,45%). Infeksi intraabominal terkait rumah sakit lebih banyak dibanding IIA komunitas yaitu 56,36%). Patogen yang paling sering ditemukan adalah Eschericia coli (34,78%), Klebsiella pneumoniae (10,86%) dan Enterococcus faecalis ((8,69%). Pola kepekaan terhadap amikasin, meropenem, ertapenem dan tigesiklin adalah 100% pada isolat E. coli, sementara piperasilin/tazobaktam lebih rendah (90,62%), seftazidim dan sefepim (68,75%). Ditemukan E. coli resisten multiobat  (62,5%), K. pneumoniae resisten multiobat (50%) dan E. faecalis resisten multi obat (50%). Terdapat hubungan antara faktor klinis sepsis dengan luaran klinis. Pemberian terapi antimikroba sebaiknya mengacu kepada rekomendasi yang dibuat berdasarkan pola kuman dan pola kepekaan setempat.

Kesimpulan: Bakteri Gram negatif masih merupakan bakteri yang paling sering ditemukan pada kasus IIA. Dengan tingginya temuan bakteri resisten multiobat makan pemberian antimikroba harus mempertimbangkan cakupan antimikroba terhadap patogen penyebab.

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Background: Intraabdominal infection (IAI) is the peritoneum inflammatory process due to microorganisms, the leading cause of sepsis, and the second cause of death in the intensive care unit (ICU). Complicated intra-abdominal infection worldwide observational study (CIAOW) showed the mortality rate of complicated IAI of 10.5%. Antimicrobial therapy or antibiotics is important in managements of IAI. Accurate information is needed to improve IAI management; regarding the characteristics and patterns of sensitivity of antibiotics as a reference for antibiotics use in IAI.

Aim: This study aims to obtain microbiological and clinical pictures in IAI and the correlation between clinical and microbiological factors with the patient’s clinical outcomes.

Method: Data were collected prospectively using a cross-sectional design. The sampel in this study is intraabdomen tissue and blood that was taken during surgery. Identification and antimicrobial sensitivity testing was carried out to detect aerob and anaerob bacteria.

Result: Complicated cases is larger in number more than non-complicated IAI (63.63%), sepsis (63.63%) and peritonitis (45.45%). Hospital-related IAI much more than community IAI (56.36%). The most common pathogens are Eschericia coli 34.78%), Klebsiella pneumoniae (10.86%) and Enterococcus faecalis (8.69%). The sensitivity of amikacin, meropenem, ertapenem and tigesycline was 100% in E. coli, while piperacillin/ tazobactam was lower (90.62%), ceftazidime and cefepime (68.75%). It was found that multi-drug E. coli was (62.5%), multi-drug resistant K. pneumoniae (50%) and multi-drug resistant E. faecalis (50%). There is correlation between sepsis clinical factors with patient’s outcome. The administration of antimicrobial therapy should refer to recommendations made based on local microba and sensitivity patterns. 

Conclusion: Gram-negative bacteria are still the most common bacteria found in patient intraabdominal infections. With the high findings of multi-drug resistant bacteria, antimicrobial administration must consider the antimicrobial coverage of the causative pathogen.

 

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"DEnaturing gradient get electrophoresis was used to identify the bacterial community at the Gedongsongo (WGS-2) hot spring....."

"Penelitian isolasi bertahap telah dilakukan untuk mendapatkan bakteri pendegradasi fraksi jenuh, aromatik, resin, dan aspal. Isolasi dilakukan terhadap lima sampel tanah terkontaminasi minyak dari Sumatera Selatan. Medium isolasi menggunakan soil extract diperkaya oil recovery atau oil recovery sisa degradasi (OSD) sebagai satu-satunya sumber karbon dan energi sesuai tahapan isolasi. OSD setiap akhir tahap isolasi difraksinasi menggunakan analisis SARA untuk mengetahui fraksi jenuh, aromatik, resin dan aspal. Hasil penelitian mendapatkan enam isolat bakteri terpilih berdasarkan kecepatan degradasi tertinggi pada setiap tahap, satu isolat bakteri pendegradasi fraksi jenuh yaitu Mycobacterium sp. T1H2D4-7 dengan laju degradasi 0,0199 mg/jam dan kepadatan 8,4x10 6cfu/g dari tahap I. Isolat T2H1D2-4 teridentifikasi sebagai Pseudomonas sp. merupakan bakteri pendegradasi fraksi aromatik dengan laju degradasi 0,0141 mg/jam dan kepadatan 5,1x10 6cfu/g diperoleh pada tahap II. Dua isolat yaitu Micrococcus sp. T3H2D4-2 dan Pseudomonas sp. T1H1D5-5 merupakan bakteri pendegradasi fraksi resin yang masing-masing mempunyai laju degradasi 0,0088 mg/jam dengan kepadatan 5,6x10 6cfu/g, dan 0,0089 mg/jam dengan kepadatan 5,7x10 6cfu/g diperoleh dari tahap III. Isolasi tahap IV diperoleh dua isolat yaitu Pseudomonas sp. T4H1D3-1 dan Pseudomonas sp. T4H3D5-4 yang merupakan bakteri pendegradasi fraksi aspal, masing-masing mempunyai kecepatan degradasi 0,0057 mg /jam dengan kerapatan 5,6x10 6cfu/g, dan 0,0058 mg/jam dengan kerapatan 5,7x10 6cfu/g.

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Sequential isolation has been conducted to obtain isolates of saturated, aromatic, resin, and asphaltene fractions degrading bacteria from oil contaminated sites. Five soil samples were collected from South Sumatera. These bacterial isolates were obtained using soil extract medium enriched with oil recovery or remaining-oil recovery degradated (ROD) as sole carbon and energy sources according to the isolation stage as the isolation medium. ROD at the end of every isolation stage analyzed oil fractions by use of the SARA analysis method. Six isolates of bacteria have been selected, one isolate was fraction satu rates degrading bacteria that are Mycobacterium sp. T1H2D4-7 at degradation rate 0.0199 mgs/h with density 8.4x10 6cfu/g from stage I. The isolate T2H1D2-4, identified as Pseudomonas sp. was fraction aromatics degrading bacteria at accelerate 0.0141 mgs/h with density 5.1x10 6cfu/g are obtained at stage II. Two isolates namely Micrococcus sp. T3H2D4-2 and Pseudomonas sp. T1H1D5-5 were fraction resins degrading bacteria by accelerate 0.00 88 mgs/h at density 5.6x10 6cfu/g and 0.0089 mgs/h at density 5.7x10 6 cfu/g are obtained at stage III. Isolation of stage IV has been obtained two isolates Pseudomonas sp. T4H1D3-1 and Pseudomonas sp. T4H3D5-4 were fraction asphaltenes degrading bacteria by accelerate 0.0057 mgs/h at density 5.6x10 6cfu/g and accelerate 0.0058 mgs/h at density 5.7x10 6cfu/g."

"Among twenty one isolates, obtained from "aren" (Aretga Rinnata) vinegar, 10 isolates were identified as acetic acid bacteria, belong to genus Acetobacter. Isolates no. 12 was used as inoculum for vinegar fermentation. Saccharomyces cerevisiae (Y-17) was provided by University of Indonesia Culture Collection.Two hundred fifty grams of pineapple (Ananas comosus) peel was boiled for 1.5 hours and then filtered to obtain the extract. Aquadest was added into substrate to obtain 1 litre of extract and then added with 15% or 20% castor sugar. Substrate was sterilised at 121°C for 10 minutes.Fermentation was carried out in syrup bottle containing 540 ml substrate. Approximately 60 ml of starter containing mix-culture with diffrent ratio of 1 day old S. cer visiae (106 cfu/ml) and 5 days old Acetobacter sp. no.12 {10 cfu/ml) was inoculated into the substrate. The ratio of yeast cells to bacteria were follow: (1:1); (2:1); (3:1} or (4:1). Fermentation was set up in room temperature (3O -- 32°C for 1 month. The concentration of acetic acid was titrated with standarised NaOH.Result of this study showed that substrate with 15% sugar yielded (1.1 - 1.4)% acetic acid. The average acetic acid concentration from substrate with 20% sugar were (0.44 - 0.89%). It was concluded that substrate with 15% sugar gave higher concentration and the best ratio of starter was (1 : 1)."

"Sugar is a very important carbon and energy source for human. Thelocal production of sugar in indonesia is not adequate and alternativesources should be found. Microorganisms (Bacillus amyfoiiquefaciens, B. Iicheniformis, B. cereus, B. circulans, B. megaterium, B. polymyxa, B. stearothermophilus, Pyrococcus woeseg P. furiosus, Clostndium thermosulfurogenes, C. thermohydrosulfuricum, Aspergillus awamorL A. nigen A. oryzae, A. saitoil Mucor rouxianus, Penicillium oxalicum, Rhizopus deleman Aerobacter aerogenes, and Streptomyces) are known as producer ot on-amylase, glucoamylase, and pullulanase enzymes through of starch fermentation which may be converted into a sugar compound. A preliminary study on endophytic bacteria proved their ability to grow on soluble starch, glutinous rice, and pullulan. Pullulanase convert pullulan to maltotriosa. This enzyme may work synergistically with on-amylase and with glucoamylase for a better conversion of starch to glucose. An endophytic bacteria lCMe3 obtained from the Research and Development Centre for Biotechnology LIP! at Cibinong, Bogor was examined on its ability to produce pullulanse _ For this purpose, soluble starch 1%, cassava starch 1%, and pullulan 1% (all wlv), were used as carbon and energy source in Bakshi medium (Bakshi etal., 1993). The concentration of the inoculum_was 1.25 x 10° cells/ml. Incubation was carried out at : 30°C (room temperature) and 37°C (Mapiliandari, 1999), at pH 7.0 (Bakshi et al., 1993) and pH 5.0 (Mapiliandari, 1999). The fermentation process was terminated after 24 - 26 hours. The growth of lCNle3 varied depending on carbon source, temperature, and pH. The best growth was found on pullulan at pH 7.0 and incubation temperature of of 30°C . However, when the pH of the medium was lowered to 5.0 (Mapiliandari, 1999) and the incubation temperature 30°C a higher cell number (79.5) x 108 cells/ml was obtained on pullclan as carbon source. The bacteri was grown on cassava starch medium and the pullulanase activity studied. The synergism of pullulanase with amylase and with glucoamylase to degrade cassava starch was also studied. To obtain the crude enzyme extract, the cell mass was centrifuged with a Sorval RC - 26 Plus centrifuge. The Hltrate was then concentrated with UHF, sedimented with (NH4)2SO4, and dialized with buffer Na-acetat (pH 4.8). Activity of the crude enzyme was examined on cassava starch and onpullulan. The unit activity of enzyme was 1.374 U/ml on cassava starch,1.290 U/ml on pullulan, and the protein content was 0.039 mglml. The activity of the crude enzyme, after treatment with UHF, was 2.225 U/ml for pullulan, 2.527 U/mt for cassava starch, and the protein content was 0.014 mg/ml. The activity of the crude enzyme obtained after sedimentation with 60% saturation of (NH4)2SO4, was 1.156 U/ml for pullulan, 1.162 U/mi for cassava starch, the protein content 0.579 mg/ml. After dialysed with buffer Na-acetate (pH 4.8) the activity was 6.25 U/ml for pullulan, 6.45 U/ml for cassava starch with the protein content of 2.997 mg/ml. To study the optimum pH and temperaturefor the enzyme production, the isolate iCMe3 was grown on Bakshi medium with various pHs, : 4.0, 4.5, 4.8, 5.0, 5.5, 6.0, 6.5, 7.0 and incubated at various temperatures 30°C, 40°C 50°C, 60°C, 70°C, 80°C, 90°C. The optimum pH for enzyme sinthesis on puliulan was 5.0 (4.81 U/ml) and on cassava starch 4.8 (13.27 U/ml). The optimum temperature for enzyme synthesis on pullulan was 40°C (26416 U/ml) and on cassava starch 50°C (22.34 U/ml). The best synergism of pullulanase with on-amylase for both C sources was 25% (dilution of enzyme), while the synergism with glucoamylase was 100% for pulluian and 50% for cassava starch to convere the starch (pullulanand cassava starch) glucose."