Latar belakang: Penuaan merupakan proses biologis yang ditandai dengan prosesdegenerasi pada sel yang menyebabkan perubahan struktur dan penurunan fungsijaringan dan organ. Prevalensi sejumlah penyakit degeneratif meningkat padakelompok lansia, salah satunya adalah penyakit organ ginjal. Biaya penangananpenyakit ginjal saat ini masih sangat mahal, sehingga dapat membebani ekonominegara dan masih menjadi suatu masalah di dunia kedokteran. Salah satumekanisme proses penuaan adalah adanya stres oksidatif. Tanaman Centellaasiatica (CA) dan Acalypha indica (AI) diketahui memiliki kandungan antioksidantinggi. Sejumlah penelitian menunjukkan kemampuan CA dan AI dalammemperbaiki kondisi stres oksidatif.Tujuan: Mengetahui pengaruh pemberian ekstrak CA dan AI terhadap gambaranhistologis glomerulus pada ginjal tikus Sprague-Dawley tua.Metode: Penelitian ini menggunakan sampel bahan biologis tersimpan berupa blokparafin jaringan ginjal dari penelitian sebelumnya. Terdapat 5 kelompok perlakuan:tikus usia muda (8-12 minggu), tikus usia tua (20-24 bulan), tikus tua + AI, tikustua + CA, dan tikus tua + Vitamin E. Perlakuan diberikan selama 28 hari sebelumterminasi tikus. Organ ginjal diproses menjadi preparat histologis denganpewarnaan hematoksilin-eosin, dan dilakukan pengukuran 3 variabel yaitu jumlahglomerulus, diameter glomerulus, dan persentase luas ruang Bowman.Hasil: Tikus usia tua mempunyai kecenderungan untuk mempunyai jumlahglomerulus yang lebih sedikit dan persentase luas ruang Bowman yang lebih kecildibanding kelompok lainnya, namun secara statistik tidak bermakna (ANOVAp=0,260 dan p=0,141). Pengukuran diameter glomerulus juga menunjukkankecenderungan tikus tua mempunyai diameter yang lebih besar (ANOVA p=0,000),tetapi tidak menunjukkan adanya perbedaan bermakna pada pemberian AI (p=0,697) maupun CA (p=0,158).Simpulan: Ekstrak Acalypha indica dan Centella asiatica tidak menyebabkanperubahan bermakna pada gambaran histologis glomerulus tikus tua. Diperlukanpenelitian lebih lanjut dengan menggunakan dosis yang lebih besar dan durasiperlakuan lebih lama untuk mengetahui efek anti-aging pada ginjal dari AI dan CA.

---

Background: Aging is a biological process marked by cellular degeneration that

causes structural changes and declining organ function. The prevalence of

degenerative diseases has increased in the elderly population, one of which is

kidney disease. The current cost for kidney diseases management is very expensive,

therefore it could burden the country’s economy, and it is still a major health

problem. One of the main underlying mechanism in the aging process is oxidative

stress. Herbal plants such as Centella asiatica (CA) and Acalypha indica (AI) are

known to have high antioxidant levels. Previous studies have shown that CA and

AI have the capability to inhibit the oxidative stress.

Purpose: To investigate the effect of Acalypha indica and Centella asiatica extract

on glomerular histology of the old Sprague-Dawley rats.

Method: We used stored biological samples of the kidney paraffin blocks taken

from the previous study. There were 5 treatment groups: younger age rats (8-12

weeks), older age rats (20-24 months), old rats + AI, old rats + CA, and old rats +

Vitamin E. Treatments were given in 28 days and then the rats were terminated.

The kidneys were processed into histological slides with hematoxylin-eosin

staining. There were 3 variables measured from the samples, which were

glomerular number, glomerular diameter, and Bowman’s space area percentage.

Result: The old rats tend to have fewer glomerular number and narrowing

Bowman’s space area percentage compared to other groups, however they are not

statistically significant (ANOVA p=0,260 dan p=0,141). The old rats also have the

tendency of bigger glomerular diameter (ANOVA p=0,000), however it is not

significantly different in AI (p=0,697) and CA treatment (p=0,158).

Conclusion: Our study showed that the extract of Acalypha indica and Centella did

not cause any histological changes in the glomerulus of the older age rats. Further

studies are needed using higher dosage and longer duration of treatment to

investigate the antiaging effect of AI and CA in the aging kidney