ABSTRAK
Latar belakang: C. albicans merupakan jamur yang dominan pada infeksi endodontikpersisten yang berperan dalam kegagalan perawatan saluran akar. Pembentukan biofilmmerupakan salah satu faktor virulensi C. albicans yang dapat meningkatkan resistensiterhadap agen antijamur. Klorheksidin 2% sebagai larutan irigasi yang efektif dalammengeliminasi biofilm C. albicans telah terbukti bersifat toksik terhadap sel-sel sehatsehingga diperlukan alternatif larutan irigasi yang efektif dan aman, yaitu berasal daribahan alami. Temulawak (Curcuma xanthorrhiza Robx.) mengandung xanthorrhizolyang terbukti bersifat antijamur. Namun, belum terdapat penelitian yang menganalisiskonsentrasi optimum xanthorrhizol dalam mengeliminasi biofilm C. albicans ATCC10231. Tujuan: Menganalisis efek antijamur xanthorrhizol 0,25%, xanthorrhizol 0,5%,xanthorrhizol 0,75%, xanthorrhizol 1%, xanthorrhizol 1,25%, dan klorheksidin 2%terhadap biofilm C. albicans ATCC 10231. Metode: Pemaparan xanthorrhizol terhadapbiofilm C. albicans ATCC 10231 dilakukan selama 15 menit, kemudian diuji denganmetode MTT assay dan hitung koloni. Hasil: Tidak terdapat perbedaan bermaknasecara statistik antara persentase eradikasi dan hasil hitung jumlah koloni pascapemaparan xanthorrhizol 1%, xanthorrhizol 1,25%, dan CHX 2% pada biofilm C.albicans ATCC 10231. Simpulan: Xanthorrhizol 1% dan xanthorrhizol 1,25%memiliki efek antijamur terhadap biofilm C. albicans ATCC 10231 yang setara denganklorheksidin 2%.

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ABSTRACT
Background: C. albicans is the most dominant fungal species in persistent endodonticinfection that has been associated with failure of endodontic treatment. An ability toform biofilm is one of the C. albicans virulence factor that increase resistance towardsantifungal agent. In endodontic, 2% chlorhexidine as an effective irrigation solutionagainst C. albicans biofilm has been proven to be toxic to healthly cells so that aneffective and safe alternative irrigation solution is needed, which is derived from naturalingredients. Java turmeric (Curcuma xanthorrhiza Robx.) contains xanthorrhizol whichhas an antifungal effect. However, no studies have analyzed the optimum concentrationof xanthorrhizol in eradicating bioflm of C. albicans ATCC 10231. Aims: To analyzeantifungal effect of 0,25% xanthorrhizol, 0,5% xanthorrhizol, 0,75% xanthorrhizol, 1%xanthorrhizol, 1,25% xanthorrhizol and 2% chlorhexidine against biofilm of C. albicansATCC 10231. Methods: fifteen minutes exposure of xanthorrhizol to biofilm of C.albicans ATCC 10231, then antifungal effect tested by MTT assay and total plate countmethod. Results: There was no statistically significant difference between percentage ofbiofilm eradication and TPC results after exposure to 1% xanthorrhizol, 1,25%xanthorrhizol, and 2% chlorhexidine in biofilm of C. albicans ATCC 10231.Conclusion: 1% xanthorrhizol and 1,25% xanthorrhizol have an antifungal effectagainst biofilm of C. albicans ATCC 10231 which is equivalent to 2% chlorhexidine.