[
ABSTRAKDoksorubisin merupakan salah satu antikanker golongan antrasiklin yang efektif,
untuk keganasan di darah. Akan tetapi, seperti antikanker konvensional pada
umumnya, penggunaan doksorubisin dapat menyebabkan berbagai efek samping pada
organ lain, misalnya pada testis sehingga penggunaannya di klinis menjadi terbatas.
Hal ini disebabkan karena mekanisme antikanker doksorubisin dapat juga
menimbulkan toksisitas pada testis. Peningkatan stress oksidatif adalah salah satu
mekanisme dapat menyebabkan kerusakan pada organ tersebut. Mangiferin sebagai
zat antioksidan alami, terkandung dalam Mangifera Indica L. diperkirakan dapat
digunakan untuk mengurangi toksisitas testis. Namun sampai saat ini, belum ada
penelitian yang mengeksplor efek proteksi mangiferin terhadap kerusakan oksidatif
testis yang diinduksi doksorubisin.
Penelitian ini menggunakan tikus jantan Sprague Dawley, yang dibagi menjadi empat
kelompok. Masing-masing kelompok terdiri dari enam ekor tikus. Tikus pada
kelompok kontrol negatif diberikan doksorubisin secara intraperitoneal (dosis total 15
mg/kgBB) dan kelompok normal diberikan NaCl 0,9%. Mangiferin (dosis 30 dan 60
mg/kg BB) diberikan oral selama tujuh minggu. Setelah, tujuh minggu tikus
dimatikan dan testis dikumpulkan untuk analisis parameter stress oksidatif biokimia
kadar MDA (malonedyaldehide), aktivitas SOD (Superoxide Dysmutase), perubahan
histologi dan apoptosis kaspase-9 dan kaspase-12. Hasil penelitian menunjukkan
bahwa pemberian doksorubisin selama dua minggu dapat meningkatkan kadar MDA,
menyebabkan kerusakan sel spermatogenik, sel Sertoli dan penciutan diameter
tubulus seminiferus testis, peningkatan ekspresi kaspase-9 di sisi luminal yang
diberikan doksorubisin. Pemberian mangiferin dosis 30 dan 60 mg/kg BB selama
tujuh minggu dapat mengurangi kerusakan sel spermatogenik dan sel Sertoli tubulus
seminiferus testis, penurunan kadar MDA dan penurunan ekspresi kaspase-9 pada
kelompok perlakuan diberikan doksorubisin dan mangiferin. Perbaikan parameterparameter
ini mengindikasikan bahwa mangiferin mempunyai efek proteksi terhadap
kerusakan sel spematogenik dan sel sertoli tubulus seminiferus testis tikus yang
diberikan doksorubisin.
ABSTRACTDoxorubicin, one of the anthracycline anticancer class, is effective especially in blood
malignancy. However, as in the general use of the conventional anticancer-drugs.
Doxorubicin can cause various side effects in other organs, such as the testes so that
its use in clinical become limited. This is because of the anticancer mechanism can
cause cytotoxicity on testes. The increased oxidative stress is the main mechanism
that can be the causal. Mangiferin as a natural antioxidant substance, contained in
Mangifera Indica L., is expected to reduce the toxicity. The Antioxidants are
expected to reduce the toxicity of the testes. But until now, no studies have explored
the effects of mangiferin protection against oxidative damage induced testicular
doxorubicin.
This study used male Sprague Dawley rats, which were divided into four groups.
Each group consisted of six mice. Rats in the negative control group was given
intraperitoneal doxorubicin (total dose 15 mg/kg) and the normal group was given
normal saline 0.9%. Mangiferin (doses of 30 and 60 mg/kg) was administered orally
for seven weeks to the treatment gtoups (both DOX and MAG were given). After
seven weeks-off, testes of mice were collected for analysis of biochemical parameters
i.e. oxidative stress levels of MDA and SOD activity, histology and apoptosis of the
caspase-9 and of the caspase-12. The results showed that administration of
doxorubicin for two-weeks can cause damage to Sertoli, spermatogenic cells and
shrinking of diameter of testicular seminiferous tubules, increasing the levels of
MDA, increasing in the expression of caspase-9 on the luminal side in the treatment
group was given doxorubicin. This possibility of the doxorubicin dose given is too
toxic to the testes in this study. Mangiferin dose administration of 30 and 60 mg / kg
for seven-weeks can reduce the damage of Sertoli and spermatogenic cells of the
testicular seminiferous tubules, decrease levels of MDA, reduce Sertoli,
spermatogenic cell and diameter of the testicular seminiferous tubulus damage,
decrease caspase-9 expression only on luminal side of the seminiferus tubulus in the
groups given both of doxorubicin and mangiferin. these parameters indicate that
mangiferin, which has antioxidant?s activity, provides protective effects against
oxidative damage in spematogenic and Sertoli cell testicular seminiferous tubules of
mice given doxorubicin, Doxorubicin, one of the anthracycline anticancer class, is effective especially in blood
malignancy. However, as in the general use of the conventional anticancer-drugs.
Doxorubicin can cause various side effects in other organs, such as the testes so that
its use in clinical become limited. This is because of the anticancer mechanism can
cause cytotoxicity on testes. The increased oxidative stress is the main mechanism
that can be the causal. Mangiferin as a natural antioxidant substance, contained in
Mangifera Indica L., is expected to reduce the toxicity. The Antioxidants are
expected to reduce the toxicity of the testes. But until now, no studies have explored
the effects of mangiferin protection against oxidative damage induced testicular
doxorubicin.
This study used male Sprague Dawley rats, which were divided into four groups.
Each group consisted of six mice. Rats in the negative control group was given
intraperitoneal doxorubicin (total dose 15 mg/kg) and the normal group was given
normal saline 0.9%. Mangiferin (doses of 30 and 60 mg/kg) was administered orally
for seven weeks to the treatment gtoups (both DOX and MAG were given). After
seven weeks-off, testes of mice were collected for analysis of biochemical parameters
i.e. oxidative stress levels of MDA and SOD activity, histology and apoptosis of the
caspase-9 and of the caspase-12. The results showed that administration of
doxorubicin for two-weeks can cause damage to Sertoli, spermatogenic cells and
shrinking of diameter of testicular seminiferous tubules, increasing the levels of
MDA, increasing in the expression of caspase-9 on the luminal side in the treatment
group was given doxorubicin. This possibility of the doxorubicin dose given is too
toxic to the testes in this study. Mangiferin dose administration of 30 and 60 mg / kg
for seven-weeks can reduce the damage of Sertoli and spermatogenic cells of the
testicular seminiferous tubules, decrease levels of MDA, reduce Sertoli,
spermatogenic cell and diameter of the testicular seminiferous tubulus damage,
decrease caspase-9 expression only on luminal side of the seminiferus tubulus in the
groups given both of doxorubicin and mangiferin. these parameters indicate that
mangiferin, which has antioxidant’s activity, provides protective effects against
oxidative damage in spematogenic and Sertoli cell testicular seminiferous tubules of
mice given doxorubicin]
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