Sintesis ramah lingkungan pada senyawa imina telah dilakukan dari turunan vanilin dan 2-hidroksi asetofenon dengan variasi kecepatan stirer dalam pelarut air. Selanjutnya, dilakukan kolom dan karakterisasi hasil produk serta uji toksisitas, antibakteri, dan antioksidan. Produk A [((E)-4-(1-(2-hydroxyphenyl) ethylidene amino)-1,5-dimethyl-2-phenyl-1H-pyrazol-3(2H)-one)] diperoleh dari turunan 2-hidroksi asetofenon pada kecepatan 250 rpm selama 24 jam. Sedangkan, vanilin menghasilkan produk B pada kecepatan 450 rpm selama 15 menit. hasil menunjukan bahwa % yield untuk produk A dan B bertutur-turut sebesar 40.68% dan 19.76%. Uji aktivitas antioksidan dan aktivitas biologi seperti: toksisitas dan antibakteri menunjukan hasil bahwa produk B lebih bersifat toksik dan aktif sebagai antioksidan dibandingkan produk A. Sementara uji aktivitas antibakteri menunjukan bahwa kedua produk tersebut memiliki respon positif terhadap bakteri S.aureus dan E.coli.

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The green synthesis of imine compound was determined from vanillin and 2-hydroxy acetophenone. It had been synthesized under varying stiring speed in water solution. After that, the process was followed by kolom and chatacterization the reaction product, which were further examinated for its Tocicity, antibacterial and antioxidant activities test. The Product A, [((E) -4- (1- (2-hydroxyphenyl) ethylideneamino) -1,5-dimethyl-2-phenyl-1H-pyrazol -3 (2H) -one)] was synthesized from 2-hidroxy acetophenon under stiring 250 rpm and 24 hour reaction time. Meanwhilevanilin was determined product B ([((E) -4- (4-hydroxy- 3- (vinyloxy) benzylideneamino) -1,5-dimethyl-2-phenyl-1H- pyrazol-3 (2H) - one)]) under stiring 450 rpm and 15 minute reaction time. The % yield of product A and B were respectively 40.68% and 19.76%. The antioxidant and biological activities like toxicity and antibacterial activity showed that the product B are toxic and more active as an antioxidant than product A. While the antibacterial activity test showed that both products have a positive response to S. aureus bacteria and E. coli bacteria.