Telah dilakukan peneiltian aktifitas antibakteriin vitro dari ekstrak kulit buah Punica granatum L. tenhadappelbagai kuman standard dan kurnan liar yang diasin.gkan dari pendenita, Penentuan aktifitas ekstrak tersebutmenggunakan 2 cara, yaitu dengan penen;tuan konsentrasihambatan minimum (MIC) dan pemerik,saan lebar zonahambatan yang dibentuk .disekelilinq cakram yang mengan -dung ekstrak kulit buah Punica granatum L.Penentuan aktifitas antibateni ekstrak kulit buahPunica gran atum L. yang, didasarkan atas besarnya NICmenggunakan metoda " Agar dilution " mernbenikan hasilKonsentrasi hambatan minimum terhadap kuman-kuman standardEschenichia coil ATCC 25922, Kiebsiella pneumoniaeATCC 13883, Pseudomonas aerugiriosa ATCC 27853, Salmonellatyphimuriurn ATCC 14028, Shigelia flexneni ATCC 12022,Shigella sonnel ATCC 25931, dan Stapylococcus aureusATCC 25923 berturut-turut adalah 625,00 mcg/ml, 312950mcg/ml, 312,50 mcg/ml, 625,00 mcg/ml, 16,2 mcg/ml,312,50 mcg/ml, dan 78 1,13 mcg/ml.Bendasarkan lebar zona hambat.an yang terbentuk disekeliling . cakrani yang mengandung. ekstrak kulit buahnica granatum L. dapat diambil kesimpulan : Aktifitas a n.tibakteni dari ekst.rak kulit. buah Pun ica granatum L. terbaik terhadap kuman Staphylococcus aureus, menyusul benturu.t-turut terhadap Shige1l flexneni, Pseudomonas aeru-Qinosa, Kiebsiella pneumoniae, Shigel.la sonnei, Escherichia coil, dan Salmonella typhimurium.

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A study' of the in'., vitro antibacterial activity of

Punica granatum L. rind extracts has been carried out

against, several standard bacterial strains and wild

strains isolated from patients applying two methods, i.e.

the determination, of the minimum inhibitory concentration.

(N1c) and the determination of the inhibition zone formed

around the disc containing extracts of Punica granatum L.

ri:nd.

The determination of the antibacterial activity of

extracts of Punica granatum L. rind using the agar dilu -

t'.ion method show the following' results (MIC/mi), respec -

t.ively : Minimum inhibitory concentration (L!c) on Es'cherichia

coil ATCC 25922 9 Kiebsiella pn;eumoniae ATCC 138.83,

Pseudomonas aerug'i'nosa ATCC 27853 9 Salmonella ty'phimurium

ATCC 14028 9 Shigella f'lexnerl A1TCC 12022 9 Shigella sonnel

ATCC 25931, and Staphylococcus aureus ATCC 25923 were

625,00 mcg/ml, 312 9 50 rncg/mi, 312,50 mcg/ml, 625,00 mcg/

ml, 156 9 25 mcg/ml, 312,50 m'cg/ml, an.d 78.,13 mcg/ml.

Based' on the size of' the inhibition zone formed

around the disc. containing, extracts of Punica Qranatum L.

rind, the following,-conclusion-could b:e drawn : The antibacterial

activity of Punica granatum L. rind extracts on

Staphy-lococcus aureus is' considered to be the best follow

ed respectively' by, Sh.lqella flexneri, Pseudomonas aeruginosa,

Klebsiella pneumoniae, Shigella sonnei, Escheric.hia

coli, and Salmonella typhimurium.